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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 曲芳華 | |
dc.contributor.author | Chia-Chen Wu | en |
dc.contributor.author | 吳家禎 | zh_TW |
dc.date.accessioned | 2021-06-13T03:45:48Z | - |
dc.date.available | 2008-06-23 | |
dc.date.copyright | 2008-06-23 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-26 | |
dc.identifier.citation | 1. 鍾振德、郭幸榮(2005)台灣肖楠之生殖週期。台灣林業科學20: 315-329
2. 應紹舜 (1999)台灣高等植物彩色圖誌 第一卷(增訂二版) 3. Bodt S. D., Raesl J., Peer Y.V.D. and Theißen G. (2003) And then there were many: MADS goes genomic. Trends in Plant Science 8: 475-483. 4. Brent R., and Ptashne M. (1985) A bracterial repressor protein or a transcriptional terminator can block upstream activation of a yeast gene. Nature 314: 198. 5. Chang, S., J. Puryear and J. Cairney (1993) A simple and efficient method for isolating RNA from Pine Trees. Plant Molecular Biology Reporter 11: 113-116. 6. Carlsbecker A., Karolina T., Urban J., Marie E. and Peter E. (2004) The MADS-box gene DAL1 is a potential mediator of the juvenile-to-adult transition in Norway spruce (Picea abies). The Plant Journal 40: 546–557. 7. Colombo, L., Franken J., Koetje E., vanWent J., Dons H.J., Angenent G.C., and Tunen A.J. (1995) The petunia MADS box gene FBP11 determines ovule identity. Plant cell 7: 1859-1868. 8. Cortines M.E., Saedler H., and So mMer H. (1999) Ternary complex formation between the MADS-box proteins SQUAMOSA, DEFICIENS and GLOBOSA is involved in the control of floral architecture in Antirrhinum majus. The EMBO journal 18: 5370-5379. 9. Ferandiz C.G. Q., Martienssen R. and Yanofsky M.F. (2000) Redundant regulation of meristem identity and plant arachitecture by FRUITFUL, APETALA1 and CAULIFLOWER. Development 127: 725-734. 10. Florin R. (1956) Nomenclatural notes on genera of living gymnosperms. Taxon 5: 188-192 11. Folter S., I mMink R.G.H., Kieffer M., Parenicova L., Henz S.R., Weigel D., Busscher M., Kooiker M., Colombo L., Kater M.M., Davies B., and Angenenta G.C. (2005) Comprehensive Interaction Map of the Arabidopsis MADS-Box Transcription Factors. The Plant Cell 17: 1424–1433. 12. Frangioni, J. V., and Neel, B.G. (1993) Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Analytical Biochemistry 210: 179-187. 13. Fukui M., Futamura N., Mukai Y., Wang Y., Nagao A. and Shinohara K. (2001) Ancestral MADS-box genes in Sugi, Cryptomeria japonica D.Don (Taxodiaceae), homologous to the B function genes in angiosperms. Plant Cell Physiology 42: 566-575. 14. Hartmann U., Susanne H., Klaus N., Ellen W., Saedler H. and Huijser P. (2000) Molecular cloning of SVP: a negative regulator of the floral transition in Arabidopsis. The plant journal 21: 351–360. 15. I mMink R.A.H., Gadella T.W.J., Ferrario S., Busscher M., and Angenent G.C. (2002) Analysis of MADS box protein-protein interactions in living plant cells. PNAS 99: 2416-2421. 16. Irish V.F. (2003) The evolution offloral homeotic gene function. BioEssays 25: 637-646. 17. Kramer E. M., Jaramillo M.A. and Stiiio V.S.D. (2004) Patterns of gene duplication and functional evolution during the diversification of the AGAMOUS subfamily of MADS box gene in angiosperms. Genetics 166: 1011-1023 18. Lim J., Moon Y.H., An G., and Jang S.K. (2000) Two rice MADS domain proteins interact with OsMADS1. Plant molecular biology 44: 513-527. 19. Longman K.A. (1976) Some experimental apptoaches to the problem of phase change in forest trees. Acta Horticulturae 56: 81-90. 20. Ng M. and Yanofsky M.F. (2001) Function and Evolution of the Plant MADS-Box Gene Family. Section of Cell and Developmental Biology 2: 186-195. 21. Pelaz S., Ditta G.S., Baumann E., Wisman E. and Yanofsky M.F. (2000) B and C floral organ identity functions require SEPALLATA MADS-box genes. Nature 405: 200-203. 22. Pinyopich A., Ditta G.S., Savidge B., Lijegren S.J., Baumann E., Wisman E., and Yanofsky M.F. ( 2003) Assessing the redundancy of MADS-box genes during carpel and ovule development. Nature 424: 85-88. 23. Saedler H., Becker A., Winter K.-U., Kirchner C. and Theißen G. (2001) MADS-box genes are involved in floral development and evolution. Acta Biochimica Polonica 48: 351-358. 24. Samach A., Hitoshi O., Scott E.G., Gray S. D., Zsuzsanna S.S., Yanofsky M.F. and George C. (2000) Distinct roles of CONSTANS target genes in reproductive development of Arabidopsis. Secience 288: 1613-1616. 25. Santelli E. and Richmond T. J. (2000) Crystal Structure of MEF2A Core Bound to DNA at 1.5 A Resolution. Journal of molecular biology 297: 437-449. 26. Sundstrom J. and Engstrom P. (2002) Conifer reproductive development involves B-type MADS-box genes with distinct and different activities in male organ primordial. The Plant Journal 31: 161-169. 27. Tandre K., Albert V.A., Sundas A., and Engstrom P. (1995) Conifer homologues to genes that control floral development in angiosperms. Plant molecular biology 27: 69-78. 28. Tandre K., Svenson M., Svensson M.E., and Engstrom P. (1998) Conservation of gene structure and activity in the regulation of reproductive organ development of conifers and angiosperms. The plant journal 15: 615-623. 29. Theißen G. (2001) Development of floral organ identity: stories from the MADS house. Current Option in Plant Biology 4: 75-85. 30. Theissen G., Becker A., Rosa A.D., Kanno A., Kim J.T., Munster T., Winter K.U. and Saedler H. (2000) A short history of MADS-box genes in plants. Plant Molecular Biology 45: 115-149. 31. Tolia N.H., and Leemor J.T. (2006) Strategies for protein coexpression in Escherichia coli. Nature methods 3: 55-64. 32. Tonaco I.A.N., Borst J.W., Vries S.C., Angenent G.C. and I mMink R.G.H. (2006) In vivo imaging of MADS-box transcription factor interactions. Journal of Experiment Botany 57: 33-42. 33. Winter K.K., Becker A., Munster T., Kim J.T., Saedler H. and Theissen G. (1999) MADS-box genes reveal that gnetophytes are more closely related to conifers than th flowering plants. PNAS 96: 7342-7347. 34. Zahn, L. M., Kong H., Mack J.H.L., Kim S., Soltis P.S., Landherr L.L., Soltis D.E., Depamphilis C.W., and Ma H. (2005) The evolution of the SEPALLATA subfamily of MADS-box genes: a preangiosperm origin with multiple duplications throughout angiosperm history. Genetics 169: 2209-2223. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32377 | - |
dc.description.abstract | 在開花植物中,花器的形成是受到A、B、C三種不同功能型之MADS-box基因控制,更也包含了其他功能型之基因參與其中,本研究從台灣特有種台灣肖楠(Calocedrus formosana)中選殖到兩個MADS-box基因:BCFMADS以及CCFMADS。透過親緣關係圖以及胺基酸序列C端區塊之相似性分析,可將BCFMADS以及CCFMADS兩基因分別歸類於B和C功能型之家族(lineages)。更進一步透過序列分析結果得知,發現BCFMADS基因與柳杉中的CjMADS1和CjMADS2基因最相近。雖然許多的研究認為柏科與杉科植物為並源之演化關係,但是由此BCFMADS的基因分析發現,此基因在台灣肖楠與日本柳杉之間有數量之差異。CCFMADS基因則在序列上面和C功能型與D功能型之基因C-terminal之區塊相似,並與其他裸子植物無太大的差異性。本研究透過轉殖阿拉伯芥來觀察其CCFMADS基因的結果進而瞭解其功能性。結果發現,CCFMADS之轉殖和DAL2之轉殖結果相似,在SHP2之轉殖結果中也有部分相似。都會影響其營養生長之狀況,如葉小,或是葉片捲曲等表現性狀。MADS-box基因中,基因並不會單獨的表現,而是透過形成同質二元體(homodimer)、異質二元體(heterodimer)或是蛋白質複合體(complex)來表現其基因的功能性,因此本研究為欲證實BCFMADS以及CCFMADS基因間可否形成同質二元體或是異質二元體,乃利用E. coli表現此二基因之蛋白質,進行胞外之pull down分析。結果顯示,由於蛋白質不能夠順利且大量的表現在可溶性蛋白中,因此本論文之結果並不能完全的證實BCFMADS與CCFMADS基因可否形成同質二元體或是異質二元體。 | zh_TW |
dc.description.abstract | In flowering plants, the formations of floral organs are controlled by different functional types of MADS-box genes, including types A, B, and C as well as other functional type genes. In this research, two MADS-box genes, BCFMADS and CCFMADS genes, were cloned from Calocedrus formosana. Through phylogenetic analyses and the similarities at specific C-terminal motifs, BCFMADS and CCFMADS genes were categorized into B-class and C-class lineages. BCFMADS gene was also confirmed identical to both CjMADS1 and CjMADS2 from Cryptomeria japonica based on sequence analysis. Although Calocedrus and Cryptomeria have evolutionally shared paraphyletic relationship, the copy numbers of BCFMADS in Calocedrus formosana are different from those of CjMADS1 and CjMADS2 in Cryptomeria japonica. However, CCFMADS specific C-terminal motif has high similarity in C and D functional type of genes, and does not show much divergence with other gymnosperms. In addition, transgenic Arabidopsis thaliana was used to observe and further determine the function of CCFMADS gene. We found that the transgene of CCFMADS is similar to transgene DAL2 and partially the same as transgene SHP2. All sense transgenic lines would affect vegetative growth, such as the occurrence of smaller and curling leaves. During the last decade, many studies have hypothesized that the MADS-box proteins could form specific dimers, which would be further assembled into tetrameric complexes to have more functions. In order to prove whether BCFMADS and CCFMADS genes can interact with each together to form either homodimer or heterodimer, we used an in vitro approach (pull down assay) to analyze protein expressions for both genes. However, since BCFMADS and CCFMADS fusion proteins cannot express in soluble from of E. coli, we cannot prove that BCFMADS and CCFMADS genes can produce either homodimer or heterodimer in this study. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:45:48Z (GMT). No. of bitstreams: 1 ntu-95-R93625020-1.pdf: 4216785 bytes, checksum: 94441cd1724a1fd19403316bd506a1d2 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 摘要…………………………………………………………………… 5
Abstract………………………………………………………………6 第一章 前言………………………………………………………8 1. 導論………………………………………………………8 2. MADS-box基因………………………………………8 3. ABC模式…………………………………………………10 4. 台灣肖楠…………………………………………………13 5. 台灣肖楠MADS-box基因之研究序言…………………13 第二章 材料與方法 …………………………………………… 15 1. 植物材料…………………………………………… 15 1.1 台灣肖楠材料……………………………………………15 1.2 基因轉殖用之阿拉伯芥………………………………………15 2. MADS-box基因之選殖與構築質體………………………15 2.1 MADS-box基因之選殖……………………………………… 15 2.2 RACE(Rapid Amplify cDNA Ends)找出UTR區域…………16 3. 核酸與蛋白質之序列分析………………………………17 4. 南方墨點分析………………………………………………17 5. 序列之親緣關係分析…………………………………17 6. 北方墨點分析…………………………………………18 7. MADS-box基因之轉殖與阿拉伯芥種子之篩選……………19 7.1 轉殖質體之構築…………………………………………19 8. 蛋白質表現載體之構築…………………………………20 8.1 GST融合蛋白(funsion protein)之質體構築……………20 8.2 His-tag蛋白之質體構築……………………………………21 8.2.1 BCFMADS之His-tag蛋白其質體構築………………………21 8.2.2 CCFMADS之His-tag蛋白其質體構築………………………22 9. MADS-box基因蛋白質純化…………………………23 10. 西方墨點分析……………………………………23 11. 蛋白質之交互作用之測定……………………………24 第三章 結果………………………………………………………25 1. BCFMADS基因與CCFMADS基因之選殖與序列分析…………25 2. BCFMADS基因與CCFMADS基因之南方墨點分析…………30 3. BCFMADS基因與CCFMADS基因之序列親緣關係分析……31 4. CCFMADS基因在台灣肖楠生殖週期之表現………………33 5. CCFMADS基因轉殖阿拉伯芥之外表型分析………………35 6. BCFMADS與CCFMADS蛋白質之表現與純化……………43 7. BCFMADS與CCFMADS蛋白質之交互作用…………………52 第四章 討論……………………………………………………. …54 1. BCFMADS基因與CCFMADS基因之選殖與序列分析………54 2. CCFMADS基因之南方墨點分析…………………………56 3. BCFMADS基因與CCFMADS基因之序列親緣關係分析……57 4. CCFMADS基因在台灣肖楠生殖週期之表現………………58 5. CCFMADS基因轉殖阿拉伯芥之外表型分析………………58 6. BCFMADS與CCFMADS蛋白質之表現與純化……………60 7. BCFMADS與CCFMADS蛋白質之交互作用………………61 第五章 結論……………………………………………………………63 第六章 參考文獻………………………………………………… …64 第七章 附錄……………………………………………………………68 附錄一、實驗材料與實驗步驟………………………………………68 附錄二、實驗primer引子對之設計………………………………88 附錄三、一般藥品之配製…………………………………………89 附錄四、一般培養基之配製………………………………………89 | |
dc.language.iso | zh-TW | |
dc.title | 台灣肖楠MADS-box基因之選殖、特性分析及其蛋白質交互作用之分析 | zh_TW |
dc.title | Molecular cloning and characterization of MADS-box gene from Calocedrus formosana and protein interaction analysis between BCFMADS and CCFMADS | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 郭幸榮,林讚標,陳振榮,鍾振德 | |
dc.subject.keyword | 台灣肖楠,MADS,開花,生殖週期, | zh_TW |
dc.subject.keyword | Calocedrus formosana,MADS,flower,reproductive cycle, | en |
dc.relation.page | 89 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-26 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
顯示於系所單位: | 森林環境暨資源學系 |
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