請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32263
標題: | 烏心石抗腐朽菌活性成分之研究 Study on Antifungal Compounds of Michelia formosana |
作者: | Chia-Che Wu 吳佳哲 |
指導教授: | 張惠婷(Hui-Ting Chang) |
關鍵字: | 烏心石,抽出物,抗腐朽菌活性,白腐菌,褐腐菌,葉子精油,Liriodenine,Annonbraine,Guaiol,Bulnesol,β-Elemol, Michelia formosana,extracts,antifungal activities,white rot fungi,brown rot fungi,leaf essential oil,liriodenine,annonbraine,guaiol,bulnesol,β-elemol, |
出版年 : | 2006 |
學位: | 碩士 |
摘要: | 烏心石(Michelia formosana (Kanehira) Masamune & Suzuki)為台灣原生樹種,屬於台灣闊葉五木之一。本研究目的為評估抽出成分對於烏心石木材耐腐朽性的影響,分析心材抽出物及葉子精油中的抗腐朽菌活性化合物。試驗結果證實心材的抗腐朽性極佳,心材經乙醇萃取後顯示試材的重量損失率明顯上升,對於腐朽菌L. betulina與L. sulphureus的抗腐朽性明顯降低,表示乙醇抽出成分對心材的抗腐朽性具有重要的影響。評估烏心石各部位乙醇抽出物抑制腐朽菌的活性,其活性依序為心材>葉部>邊材>樹皮,證實心材的乙醇抽出物具有良好的抗腐朽菌活性。
進一步評估心材抽出物中各可溶部的活性,以正己烷可溶部的抗菌活性最佳,其次是正丁醇可溶部、乙酸乙酯可溶部及水可溶部,顯示具抑制腐朽菌活性的心材抽出成分主要存在於正己烷可溶部。進一步由活性次分離部中分離鑑定一種倍半萜類化合物及二種生物鹼,分別是(+)-epi-Cubenol 、Annonbraine及Liriodenine。其中(+)-epi-Cubenol 與Annonbraine為首次由烏心石中分離所得之天然化合物。分析Liriodenine的抗腐朽菌活性,發現Liriodenine對L. betulina與T. versicolor等白腐菌的IC50分別為0.76與3.50 μg/mL;而對L. sulphureus、G. trabeum與F. pinicola褐腐菌的IC50均低於2 μg/mL,顯示Liriodenine具有極佳的抗腐朽菌活性。 烏心石葉精油亦具有抗腐朽菌活性,由活性分離部獲得五種化合物分別是4, 5-Epoxy-β-caryophyllene、Linalool、Guaiol、Bulnesol及β-Elemol,其中以Guaiol、Bulnesol及β-Elemol的活性較佳,其中,Guaiol與β-Elemol對L. betulina白腐菌與L. sulphureus褐腐菌的半數抑制濃度均低於50 μg/mL。 綜合上述試驗結果,本試驗由烏心石心材萃取物及葉子精油中共獲得4種具抗腐朽菌活性的成分―Liriodenine、Guaiol、Bulnesol及β-Elemol,可以有效抑制白腐菌與褐腐菌的生長,值得進一步的開發與利用。 Michelia formosana (Kanehira) Masamune & Suzuki is one of the five precious hardwood trees in Taiwan. The objectives of this study were to evaluate the influence of extractives on the decay resistance of M. formosana wood and analyze the antifungal compounds of heartwood extract and leaf oil. After decay tests with white rot fungus Lenzites betulina and brown rot fungus Laetiporus sulphureus, weight losses of heartwood specimens were less than 2% against 2 kinds of rot fungi. Heartwood demonstrated high decay resistance against wood rots. However, weight losses of extracted heartwood specimens were increased significantly after decay test. It indicated that the ethanolic extract had a strong influence on the decay resistance of heartwood. Antifungal activities of ethanolic extracts obtained from M. formosana were in the following order: heartwood > leaf > sapwood > bark. Result inferred that the heartwood ethanolic extract exhibited the highest antifungal activity. Further more, antifungal activities of n-hexane, EtOAc, n-BuOH and H2O-soluble fractions obtained from heartwood ethanolic extract were assessed, the activities observed were in the order of n-hexane > n-BuOH > EtOAc > H2O-soluble fractions. These results indicated that the antifungal compounds mainly existed in the n-hexane-soluble fraction. One sesquiterpenoid, (+)-epi-cubenol, and two alkaloids, liriodenine and annonbraine, were isolated from active subfractions. (+)-epi-Cubenol and annonbraine were isolated from M. formosana for the first time. According to the antifungal test of liriodenine, IC50 values of liriodenine against white rot fungi L. betulina and T. versicolor were 0.76 and 3.50 μg/mL, respectively. And IC50 values of liriodenine against brown rot fungi L. sulphureus, G. trabeum and F. pinicola were all lower than 2.0 μg/mL. It demonstrated that liriodenine has an excellent antifungal activity. Five compounds, 4, 5-epoxy-β-caryophyllene, linalool, guaiol, bulnesol and β-elemol, were isolated from the active fractions of leaf essential oil, which also possesses antifungal activity. Amoung these compounds, guaiol, bulnesol and β-elemol have better antifungal activities against white rot fungus L. betulina and brown rot fungus L. sulphureus. According to the above results, four antifungal compounds, liriodenine, guaiol, bulnesol and β-elemol, were obtained from heartwood extract and leaf essential oil of M. formosana. These compounds which could effectively inhibit the growth of white rot fungi and brown rot fungi, are of great potential as natural wood preservatives. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32263 |
全文授權: | 有償授權 |
顯示於系所單位: | 森林環境暨資源學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-95-1.pdf 目前未授權公開取用 | 2.65 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。