請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32156
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳益群(Yi-Chun Wu) | |
dc.contributor.author | Chi-Hua Li | en |
dc.contributor.author | 李季樺 | zh_TW |
dc.date.accessioned | 2021-06-13T03:34:16Z | - |
dc.date.available | 2008-07-28 | |
dc.date.copyright | 2006-07-28 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-26 | |
dc.identifier.citation | Aboobaker A., Blaxter M. (2003). Hox gene evolution in nematodes: novelty conserved. Curr. Opin. Gene. Dev. 13, 593-598.
Akakura S., Kar B., Singh S., Cho L., Tibrewal N., Sanokawa-Akakura R., Reichman C., Ravichandran K.S., Birge R. (2005). C-terminal SH3 domain of CrkII regulates the assembly and function of the DOCK180/ELMO Rac-GEF. J. Cell. Phys. 204, 344-351. Böse J., Gruber A.D., Helming L., Schiebe S., Wegener I., Hafner M., Beales M., Köntgen F., Lengeling A. (2004). The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal. J. Biol. 3, 15. Brenner S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71-94. Brugnera E., Haney L., Grimsley C., Lu M., Walk S.F., Tosello-Trampont A-C., Macara I.G., Madhani H., Fink G.R., Ravichandran K.S. (2002). Unconventional Rac-GEF activity is mediated through the Dock180-ELMO complex. Nat. Cell Biol. 4, 574-582. Brunschwig, K., Wittmann C., Schnabel R., Bürglin T.R., Tobler H., Müller F. (1999). Anterior organization of the Caenorhabditis elegans embryo by the labial-like Hox gene ceh-13. Development 126, 1537-1546. Callahan M.K., Williamson P., Schlegel R.A. (2000). Surface expression of phosphatidylserine on macrophages is required for phagocytosis of apoptotic thymocytes. Cell Death Differ. 7, 645-653. Callebaut I., Mignotte V., Souchet M., Mornon J.P. (2003). EMI domains are widespread and reveal the probable orthologs of the Caenorhabditis elegans CED-1 protein. Biochem. Biophys. Res. Commun. 300, 619-623. Chisholm A. (1991). Control of cell fate in the tail region of C. elegans by the gene egl-5. Development 111, 921-932. Cikala M., Alexandrova O., David C.N., Pröschel M., Stiening B., Cramer P., Böttger A. (2004). The phosphatidylserine receptor from Hydra is a nuclear protein with potential Fe(II) dependent oxygenase activity. BMC Cell Biol. 5, 26. Clark S.G., Chisholm A.D., Horvitz H.R. (1993). Control of cell fate in the central body region of C. elegans by the homeobox gene lin-39. Cell 74, 43-55. Clissold P.M., Ponting C.P. (2001). JmjC: cupin metalloenzyme-like domains in jumonji, hairless and phospholipase A2β. Trends Biochem. Sci. 26, 7-9. Cui P., Baoming Q., Liu N., Pan G., Pei D. (2004). Nuclear localization of the phosphatidylserine receptor protein via multiple nuclear localization signals. Exp. Cell. Res. 293, 154-163. Ellis R.E., Jacobson D.M., Horvitz H.R. (1991a). Genes required for the engulfment of cell corpses during programmed cell death in Caenorhabditis elegans. Genetics 129, 79-94. Ellis R.E., Yuan J., Horvits, H.R. (1991b). Mechanisms and functions of cell death. Annu. Rev. Cell Biol. 7, 663-698. Fadok V.A., Voelker D.R., Campbell P.A., Cohen J.J., Bratton D.L., Henson P.M. (1992). Exposure of phosphatidylserine on the surface of apoptotic lymphocytes triggers specific recognition and removal by macrophages. J. Immunol. 148, 2207-2216. Fadok, V.A., Bratton D.L., Courtney Frasch C.L., Warner M.L., Henson P.M. (1998). The role of phosphatidylserine in recognition of apoptotic cells by phagocytes. Cell Death Differ. 5, 551-562. Fadok V.A., Bratton D.L., Rose D.M., Pearson A., Ezekewitz R.A.B., Henson P.M. (2000). A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature 405, 85-90. Fadok V.A., de Cathelineau A., Daleke D.L., Henson P.M., Bratton D.L. ( 2001). Loss of phospholipid asymmetry and surface exposure of phosphatidylserine is required for phagocytosis of apoptotic cells by macrophages and fibroblasts. J. Biol. Chem. 276, 1071-1077. Grimsley C.M., Kinchen J.M., Tosello-Trampont A-C., Brugnera E., Haney L.B., Lu M., Chen Q., Schubert D., Klingele D., Hengartner M.O., Ravichandran K.S. (2004). Dock180 and ELMO1 proteins cooperate to promote evolutionarily conserved Rac-dependent cell migration. J. Biol. Chem. 279, 6087-6097. Gumienny T.L., Lambie E., Hartwieg E., Horvitz H.R., Hengartner M.O. (1999). Genetic control of programmed cell death in the Caenorhabditis elegans hermaphrodite germline. Development 126, 1011-1022. Gumienny T.L., Brugnera E., Tosello-Trampont A-C, Kinchen J.M., Haney L.B., Nishiwaki K., Walk S.F., Nemergut M.E., Macara I.G.. Francis R., Schedl T., Qin Y., Aelst L.A., Hengartner M.O., Ravichandran K.S. (2001). CED-12/ELMO, a novel member of the CrkII/Dock180/Rac pathway, is required for phagocytosis and cell migration. Cell 107, 27-41. Hamon Y., Broccardo C., Chambenoit O., Luciani M.F., Toti F., Chaslin S., Freyssinet J.M., Devaux P.F., McNeish J., Marguet D., Chimini G.( 2000). ABC1 promotes engulfment of apoptotic cells and transbilayer redistribution of phosphatidylserine. Nat. Cell Biol. 2, 399-406. Hedgecock E.M., Sulston, J.E., Thomson J.N. (1983). Mutations affecting programmed cdll deaths in the nematode Caenorhabditis elegans. Science 220, 1277-1279 Hoeppner D.J., Hengartner M.O., Schnabel R. (2001). Engulfment genes cooperate with ced-3 to promote cell death in Caenorhabditis elegans. Nature 412, 202-206. Hong JR., Lin GH., Lin CJF., Wang WP., Lee CC., Lin TL., Wu JL. (2004). Phosphatidylserine receptor is required for the engulfment of dead apoptotic cells and for normal embryonic development in zebrafish. Development 131, 5417-5427. Kamath R.S., Martinez-Campos, M., Zipperlen P., Fraser A.G., Ahringer J. (2000). Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol. 2, RESEARCH 0002. Kamath R.S., and Ahringer J. (2003). Genome-wide RNAi screening in C. elegans. Methods 30, 313-321. Kinchen J.M., Cabello J., Klingele D., Wong K., Feichtinger R., Schnabel H., Schnabel R., Hengartner M.O. (2005). Two pathways converge at CED-10 to mediate actin rearrangement and corpse removal in C. elegans. Nature 343, 93-98. Klemke R.L., Leng J., Molander R., Brooks P.C., Vuori K., Cheresh D.A. (1998). CAS/Crk coupling serves as a “molecular switch” for induction of cell migration. J. Cell Biol. 23, 961-972. Krahling S., Callahan M.K., Williamson P., Schlegel R.A. (1999). Exposure of phosphatidylserine is a general feature in the phagocytosis of apoptotic lymphocytes by macrophages. Cell Death Diff. 6, 183-189. Kunisaki Y., Masuko S., Noda M., Inayoshi A., Sanui T., Harada M., Sasazuki T., Fukui Y. (2004). Defective fetal liver erythropoiesis and T-lymphopoiesis in mice lacking the phosphatidylserine receptor. Blood 103, 3362-3364. Li M.O., Sarkisian M.R., Mehal W.Z., Rakic P., Flavell R.A. (2003). Phosphatidylserine receptor is required for the clearance of apoptotic cells. Science 302, 1560-1563. Li S., Armstrong C.M., Bertin N., Ge H., Milstein S., Boxem M., Vidalain P-O., Han J-D J., Chesneau A., et al., Vidal M. (2004). A map of the interactome network of the metazoan C. elegans. Science 303, 540-543. Liu H., Strauss T.J., Potts M.B., Cameron S. (2005). Direct regulation of egl-1 and of programmed cell death by the Hox protein MAB-5 and by CEH-20, a C. elegans homolog of Pbx1. Development 133, 641-640. Liu Q.A., Hengartner M.O. (1998). Candidate adaptor protein CED-6 promotes the engulfment of apoptotic cells in C. elegans. Cell 93, 961-972. Lu M., Kinchen J.M., Rossman K., Grimsley C., deBakker C., Brugnera E., Tosello-Trampont A-C., Haney L.B., Klingele D., Sondek J., Hengartner M.O., Ravichandran K.S. (2004). PH domain of ELMO functions in trans to regulate Rac activation via Dock180. Nat. Stru. Mol. BIol. 11, 756-762. Luciani M-F, Chimini G.. (1996). The ATP binding cassette transporter ABC1, is required for the engulfment of corpses generated by apoptotic cell death. EMBO J. 15, 226-235. Mann R.S., Affolter M. (1998). Hox proteins meet more partners. Curr. Opin. Gene. Dev. 8, 423-429. Martin S.J., Reutelingsperger C.P.M., McGahon A.J., Rader J.A., van Schie R.C.A.A., LaFace D.M., Green D.R. (1995). Early redistribution of plasma membrame phosphatidylserine is a general feature of apoptosis regardless of the initiation stimulus: inhibiton by overexpression of Bcl-2 and Abl. J. Exp. Med. 182,1545-1556. Massol P., Montcourrier P., Guillemot J.C., Chavrier P. (1998). Fc receptor-mediated phagocytosis requires CDC42 and Rac1. EMBO J. 17, 6219-6229. Mitchell J.E., Cvetanovic M., Tibrewal N., Patel V., Colamonici O.R., Li M.O., Flavell R.A., Levine J.S., Birge R.B., Ucker D.S. (2006). The presumptive phosphatidylserine receptor is dispensable for innate anti-inflammatory recognition and clearance of apoptotic cells. J. Biol. Chem. 281, 5718-5725. Moens C.B., Selleri L. (2006). Hox cofactors in vertebrate development. Dev. Biol. 291, 193-206. Reddien P.W., Camerson S., Horvitz H.R. (2001). Phagocytosis promotes programmed cell death in C. elegans. Nature 412, 198-202. Reddien P.W., Horvitz H.R. (2000). CED-2/CrkII and CED-10/Rac control phagocytosis and cell migration in Caenorhabditis elegans. Nat. Cell Biol. 2, 131-136. Reddien P.W., Horvitz H.R. (2004). The engulfment process of programmed cell death in Caenorhabditis elegans. Annu. Rev. Cell Dev. Biol. 20, 193-221. Ridley A.J., Paterson H.F., Johnston C.L., Diekmann D., Hall A. (1992). The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 70, 401-410. Robertson A.M.G., Thomson J.N. (1982). Morphology of programmed cell death in the ventral nerve cord of C. elegans larvae. J. Embryol. Exp. Morphol. 67, 89-100. Savill J., Fadok V., Henson P., Haslett C. (1993). Phagocyte recognition of cells undergoing apoptosis. Immunol. Today 14, 131-136. Savill J., Fadok V. (2000). Corpse clearance defines the meaning of cell death. Nature 407, 784-788. Schlegel R.A., Callahan M., Krahling S., Pradhan D., Williamson P. (1996). Mechanisms for recognition and phagocytosis of apoptotic lymphocytes by macrophages. Adv. Exp. Med. Biol. 406, 21-28. Sharkey M., Graba Y., Scott M. (1997). Hox genes in evolution: protein surfaces and paralog groups. Trends. Genet. 13, 145-151. Simmer F., Tijsterman M., Parrish S., Koushika S.P., Nonet M.L., Fire.A., Ahringer J., Plasterk R.H.A. (2002). Loss of the putative RNA-directed RNA polymerase RRF-3 makes C. elegans hypersensitive to RNAi. Curr. Biol. 12, 1317-1319. Su H.P., Nakada-Tsukui K., Tosello-Trampont A-C., Li Y., Bu G., Henson P.M., Ravichandran K.S. (2002). Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP). J. Biol. Chem. 277, 11772-11779. Sulston J.E., Horvitz H.R. (1977). Post-embryonic cell lineages of the nematode, Caenorhabditis elegans. Dev. Biol. 56, 110-156. Sulston J.E., Schierenberg E., White J.G., Thomson J.N. (1983). The embryonic cell lineage of the nematode Caenorhabditis elegans. Dev. Biol. 100, 64-119. Van Auken K., Weaver D.C., Edgar L.G., Wood W.B. (2000). Caenorhabditis elegans embryonic axial patterning requires two recently discovered posterior-group Hox genes. Proc. Nat. Aca. Sci. 97, 4499-4503. Wang X., Wu YC., Fadok V.A., Lee MC., Gengyo-Ando K., Cheng LC., Ledwich D., Hsu PK., Chen JY., Chou BK., Henson P., Mitani S., Xue D. (2003). Cell corpse engulfment mediated by C. elegans phosphatidylserine receptor throuth CED-5 and CED-12. Science 302, 1563-1566. Weitzman J.B. (2004). The curious world of apoptotic cell clearance. J. Biol. 3, 13. Williamson P., Schlegel R.A. (2004). Hide and seek: the secret identity of the phosphatidylserine receptor. J. Biol. 3, 14. Wu YC., Horvitz H.R. (1998a). The C. elegans cell corpse engulfment gene ced-7 encodes a protein similar to ABC transporters. Cell 93, 951-960. Wu YC., Horvitz H.R. (1998b). C. elegans phagocytosis and cell-migration protein CED-5 is similar to human DOCK180. Nature 392, 501-504. Wu YC., Tsai MC., Cheng LC., Chou CJ., Weng NY. (2001). C. elegans CED-12 acts in the conserved CrkII/DOCK180/Rac pathway to control cell migration and cell corpse engulfment. Dev. Cell 1, 491-502. Yin J., Haney L., Walk S., Zhou S., Ravichandran K.S., Wang W. (2004). Nuclear localization of the DOCK180/ELMO complex. Arch. Bioc. Biop. 429, 23-29. Yu, X., Odera S., Chuang CH., Lu N., Zhou Z. (2006). C. elegans Dynamin mediates the signaling of phagocytic receptor CED-1 for the engulfment and degration of apoptotic cells. Dev. Cell 10, 743-757. Zhou Z., Caron E., Hartwieg E., Hall A., Horvitz H.R. (2001a). The C. elegans PH domain protein CED-12 regulates cytoskeletal reorganization via a Rho/Rac GTPase signaling pathway. Dev. Cell 1, 477-489. Zhou Z., Hartwieg E., Horvitz H.R. (2001b). CED-1 is a transmembrane receptor that mediates cell corpse engulfment in C. elegans. Cell 104, 43-56. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/32156 | - |
dc.description.abstract | 在多細胞生物的發育過程中,部分細胞會進行計劃性細胞死亡,這些凋亡的細胞必須被迅速地辨識及吞噬分解,以免引起發炎和自體免疫反應。在線蟲,已發現數個基因與吞噬作用有關,它們分成兩條功能部分重疊的路徑:ced-1,ced-6,ced-7和dyn-1路徑以及ced-2,ced-5,ced-12路徑,ced-10的功能則位在這兩條路徑的下游。吞噬作用起始於吞噬細胞上的受器,辨認死細胞表面上的“eat me”signal。在細胞凋亡的過程中,原本只會出現在細胞膜內層的phosphatidylserine (PS),會翻轉到細胞膜外層,作為一個“eat me”signal,被phosphatidylserine receptor (PSR) 辨識。在線蟲,psr-1 已被證實參與在ced-2,ced-5,ced-12這條路徑 (Wang et al., 2003)。在本篇研究,我們發現PHP-3這個posterial Hox 轉錄因子,其與PSR-1的intracellular區域和extracellular區域都會形成interaction。將php-3基因knockout和knockdown都會影響胚胎發育期間死細胞屍體的數目。php-3突變株的細胞屍體持續時間也有增長的現象。php-3和psr-1的雙重突變分析顯示這兩個基因可能位在同一條功能路徑。由php-3和ced-1,ced-2,ced-5,ced-6,ced-7,ced-10,和ced-12雙重突變分析的結果顯示:php-3突變會降低ced-1,ced-2和ced-7突變株的細胞屍體數目,對於ced-5,ced-6,ced-10和ced-12突變株則無影響,所以php-3 可能與ced-1,ced-2,和ced-7有拮抗效應。 | zh_TW |
dc.description.abstract | Programmed cell death (PCD) plays an important role in the development of multicellular organisms. After cells undergo PCD, the cell corpses are recognized and engulfed by engulfing cells. In C. elegans, there are two partially redundant pathways for engulfment: ced-1/ced-6/ced-7/dyn-1, and ced-2/ced-5/ced-12. These two pathways converge at ced-10. During apotosis, phosphatidylserine (PS), which is normally restricted to the inner leaflet of the plasma membrane, is exposed on the surface of apoptotic cells and act as an “eat me” signal to trigger phagocytosis. In C. elegans, the phosphatidylserine receptor PSR-1 binds cells with exposed PS and acts upstream in the ced-2/ced-5/ced-12 pathway (Wang et al., 2003). In this study, we found that PHP-3, a posterior Hox protein, can interacts with both intracellular and extracellular region of PSR-1. Like psr-1 embryos, php-3 mutants have more corpse number and longer cell-corpse-duration time than wild type. Genetic analysis of psr-1; php-3 double mutants suggest that psr-1 and php-3 likely act in the same pathway during programmed cell death. Double mutant analysis with previously identified engulfment genes showed that the php-3 mutation reduced the persistent corpse number of ced-1, ced-2 and ced-7 mutants, but not ced-5, ced-6, ced-10 and ced-12 mutants, suggesting that php-3 may have antagonistic effect with ced-1, ced-2 and ced-7. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:34:16Z (GMT). No. of bitstreams: 1 ntu-95-R93b43009-1.pdf: 1325225 bytes, checksum: 13cde941f6660d2fc2c0d0bf94de14ae (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 中文摘要 2
英文摘要 3 前言 4 材料與方法 12 實驗結果 15 php-3缺失會影響到胚胎發育期間細胞屍體的數目 15 php-3(RNAi) 會影響到胚胎發育期間細胞屍體的數目 15 php-3會影響胚胎發育早期細胞屍體的持續時間 15 在yeast-two hybrid system,PSR-1的intracellular區域和 extracellular區域都可與PHP-3形成interaction 16 php-3可能與psr-1共同作用影響吞噬的進行 17 php-3突變會降低ced-1,ced-2和ced-7突變株的細胞屍體數目 17 討論 19 參考文獻 22 圖表 29 附圖 42 | |
dc.language.iso | zh-TW | |
dc.title | 功能分析線蟲php-3在細胞屍體吞噬過程的角色 | zh_TW |
dc.title | Functional Assay of php-3 in Cell Corpse Engulfment in C. elegans | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃偉邦(Wei-Pang Huang),陳瑞華(Ruei-Hwa Chen) | |
dc.subject.keyword | 計畫性細胞死亡,吞噬, | zh_TW |
dc.subject.keyword | programmed cell death,engulfment,Hox gene,php-3, | en |
dc.relation.page | 42 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-27 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
顯示於系所單位: | 分子與細胞生物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-95-1.pdf 目前未授權公開取用 | 1.29 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。