乳牛孕酮檢測用生物晶片之研發

Abstract

本研究成功的利用各種技術，設計並製作出一可檢測乳牛孕酮的生物晶片。將台大動物科學技術系所用之孕酮檢測套組之實驗步驟重現於晶片上，縮短實驗時間縮短並節省實驗藥劑用量。實驗藥劑進量後，製作出的晶片能夠利用差時性的進流方式，經由蠕動幫浦驅動依序進流是反應區，並利用分光光度計讀取其濃度，達到一般免疫分析法的實驗結果。 晶片製作部份，本研究經過實驗後決定以疏水性矽膠類的聚二甲基矽氧烷(Poly-dimethylsiloxane, PDMS)為製作晶片之材料，晶片中有五個同時進流的孔道，分別載有不同的緩衝液，利用流道的長度差距做出順序反應的效果，並在確定其抗體吸附狀況後，在晶片上發生酵素型免疫分析反應。 最後，本研究成功地整合生物晶片製作技術與酵素型免疫分析法，製作一差時性進流檢測晶片以取代傳統耗時的實驗。此生物晶片具有省時、低成本、高靈敏度及高鑑別度(Selectivity)之功能，對未來酪農戶掌握乳牛發情狀況提供了一種可攜帶式、便利且穩定的工具。

This research has successfully used all the technology to design and produce a biological chip. It reconstructs the experiment procedure of the Progesterone test kit used by Department of Animal Science and Techonolgy, NTU, which reduces the experiment time and dose. After the experiment dose is given, the chip can achieve the same result in general Enzyme-immunoassay by using a designed way, which allows the dose to enter the reaction zone under the control of the peristalsis pump, and by using the Spectrophotometer to figure out the concentration of the dose. After the experiment, we decided to use Polydimethylsiloxane (PDMS) as the material to make the chip. Each chip has five passages, respectively carrying different buffer solution. With different lengths of the passages, the Enzyme-immunoassay can occur in the chip after the absorption of the antibodies has been verified.

Finally, by successfully integrating biological chip technology with Enzyme-immunoassay, this research has produced a biochip to replace the traditional, time-consuming experiment. This chip is time-saving, low-cost, highly sensitive and selective. It provides the future dairy farmers who wish to monitor their cows during the rut with a portable, convenient and stable tool.