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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31926
標題: | 以動物模式評估
液態靈芝產品對免疫功能的調節 The Immune Regulatory Effects of Liquid Ganoderma Products in Mice |
作者: | Chi-Hsuan Huang 黃繼萱 |
指導教授: | 林璧鳳 |
關鍵字: | 靈芝,免疫調節,動物模式, Ganoderma,immune modulation,murine model, |
出版年 : | 2006 |
學位: | 碩士 |
摘要: | 中文摘要
靈芝自古以來,即常被用於預防和治療許多的疾病,視其為滋補強壯、固本扶正的珍貴中草藥,在中國最早的藥物學經典「神農本草經」中,即將靈芝列為上品,明朝李時珍所著的「本草綱目」中亦提到靈芝,稱其「主胸中結,益心氣、補中、增智慧、不忘,久食輕身不老,延年神仙」。在現代的科學研究當中,已陸續發現其各種生理活性,而關於免疫調節的相關研究中,多為in vitro的實驗,因此在本研究中,即以動物模式評估靈芝產品的免疫調節功效,期望能在生物體中看到有全面性調節免疫反應的結果;本研究中使用的兩種靈芝產品分別為Ganoderma tsugae (A) 和Ganoderma lucidum (B) 的菌絲體發酵液產品。在非特異性模式中,將鼠齡八週大的BALB/c小鼠隨機分為七組,以管餵方式給予各組不同劑量之液態靈芝產品,劑量分別為每天每隻小鼠給予6 mg (A1/B1組)、12 mg (A2/B2組) 和60 mg (A10/B10組),餵食六週後進行犧牲;而特異性的模式中,Ganoderma lucidum產品管餵的劑量不變,Ganoderma tsugae產品的劑量則提高為每天每隻小鼠給予12 mg (A2組) 、60 mg (A10組) 和120 mg (A20組),餵食期間同時以OVA進行致免,同樣在餵食六週後進行犧牲。實驗結果顯示,G. tsugae產品可以降低體內氧化壓力,活化吞噬細胞活性,增加血清中IgA、IgM的含量,促進脾臟細胞分泌細胞激素的能力,並具有抗原特異性之調節作用,且能活化Peyer’s patch;而在G. lucidum產品的結果中,同樣可以看到其對於體內氧化壓力、吞噬細胞活性、血清中抗體、脾臟細胞分泌細胞激素能力及活化Peyer’s patch的功效,且除此之外,還可以促進自然殺手細胞的活性,對於腹腔細胞也有免疫調節之作用。由以上結果顯示,經由口服液態靈芝產品的方式,在動物模式中的確看到其具有免疫調節之功效;然而,其中的大分子活性物質,如多醣體,如何藉由口服的方式,經過腸道進而調節全身免疫反應的機制,仍需要更多研究進一步的探討。 ABSTRACT In the oldest Chinese medical text, “Shen-Nong Ben Cao Jin,” Ganoderma species (a group of medical fungus) is recorded as the king of herbs, and has been used for a long time in China to prevent and treat human diseases. Various properties of Ganoderma, including those regarding immune modulation, have been revealed and studied by modern science. However, many studies were restricted to the range of in vitro experiments. In this study, a BALB/c murine model was used and the systemic immune-modulation effect of Ganoderma products in vivo was expected. The samples used in the study were liquid products of fermented mycelia from Ganoderma tsugae (A) and Ganoderma lucidum (B). In the nonspecific model, eight-week-old female BALB/c mice were randomly divided into seven groups, and sacrificed after a 6-week period of tube-feeding with various doses of the Ganoderma product A or B. The doses were 6 mg (group A1/B1), 12 mg (group A2/B2), and 60 mg (group A10/B10) per day per mouse. In the specific model, BALB/c mice were immunized with OVA during the tube-feeding period, and then sacrificed as in the nonspecific model. Meanwhile, doses of the G. tsugae product were changed to 12 mg (group A2), 60 mg (group A10), and 120 mg (group A20) per day per mouse. The results showed that the G. tsugae product could reduce oxidative stress, improve phagocyte activities, increase serum IgA and IgM levels, enhance secretions of nonspecific and OVA-specific cytokines by splenocytes, and activate Peyer’s patch. The similar results were shown in the G. lucidum product in addition to NK cell activity and peritoneal cell regulations. These results support that orally administrated Ganoderma products posses systemic immune-modulation effects in BALB/c murine model. Still, how the orally administrated physiological active components, such as polysaccharides, in Ganoderma products could modulate systemic immune response through digestive gut tract remains unsolved question that requires further studies for elucidation. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31926 |
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顯示於系所單位: | 微生物學科所 |
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