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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳小梨(Show-Li Chen) | |
| dc.contributor.author | Chia-I Lee | en |
| dc.contributor.author | 李佳怡 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:24:05Z | - |
| dc.date.available | 2014-10-07 | |
| dc.date.copyright | 2011-10-07 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-07-29 | |
| dc.identifier.citation | Adams MD, Sekelsky JJ (2002) From sequence to phenotype: reverse genetics in Drosophila melanogaster. Nat Rev Genet 3: 189-198
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31907 | - |
| dc.description.abstract | BCAS2 (Breast cancer amplified sequence 2) 為一核蛋白質，在先前研究指出BCAS2可做為雌激素受體在調控基因表現時的輔助因子。本實驗室發現BCAS2可以直接與抑癌蛋白質p53結合，調控p53的基因轉錄活性與p53蛋白質的穩定性。在p53表現正常的細胞株中默化BCAS2會造成細胞凋亡，而在p53表現異常的細胞株中默化BCAS2則會造成細胞生長遲緩，顯示BCAS2可能是細胞正常生長必須的蛋白質。我們實驗室的研究亦指出BCAS2是參與核醣核酸剪切次體Prp19p/CDC5L splicing complex的核心成員之一。且增加BCAS2蛋白質的表現可以增加核醣核酸剪輯的效率。此外，我們也發現BCAS2的C端具有兩個coiled-coil domain是核醣核酸剪輯的功能性區段，而相反的，BCAS2的N端則無法有助於核醣核酸剪輯的進行。因此我的研究是利用果蠅系統發現BCAS2的發育功能。
我們發現降低果蠅體內BCAS2相似蛋白質CG4980的表現會造成果蠅無法發育為成蟲，顯示CG4980為果蠅發育的必須蛋白質。此外，雖然BCAS2與CG4980只有約59%的胺基酸序列相似度，但是BCAS2的表現可以回復默化CG4980所造成的損傷，顯示BCAS2可能在果蠅體內執行與CG4980相似的功能。然而無論是表現BCAS2 N端或是BCAS2 C端的蛋白質區段都無法完全回復默化CG4980所造成的損害，顯示在生物體中，BCAS2的N端及C端都具有重要性，必須同時存在才能執行完整功能。 | zh_TW |
| dc.description.abstract | Breast Cancer Amplified Sequence 2(BCAS2) is a 26 kDa nuclear protein, which was recently reported as a transcriptional cofactor that enhances estrogen receptor–mediated gene expression. Our lab data shows that BCAS2 can stabilize AR and enhance AR transcriptional activity. We also found that depletion of BCAS2 cause apoptosis in p53 wild-type cell lines and cause growth arrest in p53-null cell lines. It has been reported that BCAS2 is one of the Prp19p/CDC5L splicing complex member. We have provide evidence that BCAS2 can regulate constitutive splicing and alternative splicing. Previous study in our lab investigated that BCAS2 C-terminal domain was a functional domain in pre-mRNA splicing, on the other hand, BCAS2 N-terminal domain acted as a dominant negative domain refers to pre-mRNA splicing. In Drosophila, BCAS2 is an essential gene since we knockdown dBCAS2 ubiquitously cause lethality. Moreover, human BCAS2 (hBCAS2) can rescue the damage phenotype caused by knockdown of Drosophila BCAS2 (dBCAS2) on wings. However, neither hBCAS2 C-terminal domain nor hBCAS2 N-terminal domain can compensate for the damage phenotype caused by knockdown of Drosophila BCAS2 (dBCAS2) on wings. These results indicate that in Drosophila, both two domains of BCAS2 are important for Drosophila development. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:24:05Z (GMT). No. of bitstreams: 1 ntu-100-R98445108-1.pdf: 1135020 bytes, checksum: 62dbb390d4e5ceaafd91ca25598fe44f (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | Table of Contents
Page 口試委員會審訂書 誌謝 中文摘要 Abstract Chapter 1 Introduction 1 1-1 Breast cancer amplified sequence 2 was a conserved protein from yeast to mammals 1 1-2 BCAS2 was a core protein in Prp19p-associated splicing complex 2 1-3 Some members of the Prp19p-associated splicing complex played an important role in Embryonic development 3 1-4 Several splicing-associated complexes formed during spliceosome assembly and disassembly 3 1-5 Domain mapping of BCAS2 in pre-mRNA splicing 4 1-5 Drosophila melanogaster 5 1-6 Specific aims 7 Chapter 2 MATERIALS AND METHODS 8 2-1 Plasmids and constructs 8 2-2 RNA extraction: 8 2-3 RT-PCR 9 2-4 Drosophila melanogaster 13 2-4-1 Fly stock 13 2-4-2 Back-crossing the injected flies 14 Chapter 3 Results 16 3-1 the amino acid sequences between human BCAS2 and Drosophila CG4980 show high similarity. 16 3-2 knockdown CG4980 in Drosophila ubiquitously causes lethality. 16 3-3 knockdown CG4980 ectopically in Drosophila causes abnormal development in wings 17 3-4 human BCAS2 can rescue abnormal development of wings in Drosophila caused by knockdown CG4980 ectopically 18 3-5 expressing human BCAS2 N-terminal domain ectopically in Drosophila shows normal development in wings 19 3-6 expressing human BCAS2 C-terminal coiled-coil domain ectopically in Drosophila shows normal development in wings 20 3-7 human BCAS2 C-terminal coiled-coil domain can slightly rescue abnormal development of wings and scutellum in Drosophila caused by knockdown CG4980 ectopically 21 3-8 human BCAS2 N-terminal domain cannot rescue abnormal development of wings in Drosophila caused by knockdown CG4980 ectopically 23 3-9 knockdown CG4980 ectopically in Drosophila showed no obvious damage on eyes by naked-eye observation. 24 Chapter 4 Discussion 25 References 31 Figures……………………………………………………………………………….35 Appendix…………………………………………………………………………….52 | |
| dc.language.iso | en | |
| dc.subject | BCAS2 | zh_TW |
| dc.subject | 果蠅 | zh_TW |
| dc.subject | 發育 | zh_TW |
| dc.subject | Drosophila | en |
| dc.subject | BCAS2 | en |
| dc.subject | development | en |
| dc.title | BCAS2在果蠅翅膀發育的功能 | zh_TW |
| dc.title | BCAS2 Role in Drosophila Wing Development | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 詹世鵬(shih-Peng chan),吳君泰(June-Tai Wu),譚婉玉(Woan-Yuh Tarn) | |
| dc.subject.keyword | BCAS2,果蠅,發育, | zh_TW |
| dc.subject.keyword | BCAS2,Drosophila,development, | en |
| dc.relation.page | 62 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-07-29 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
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