請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31673
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蘇南維(Nan-Wei Su) | |
dc.contributor.author | Tzung-Yuan Chen | en |
dc.contributor.author | 陳琮元 | zh_TW |
dc.date.accessioned | 2021-06-13T03:17:14Z | - |
dc.date.available | 2008-08-09 | |
dc.date.copyright | 2006-08-09 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-28 | |
dc.identifier.citation | [文獻編排方式以字母為順序]
AOAC, 1984. Official methods of analysis of association of official analytical chemists. 14th ed. Washington D.C., USA Aaby, K., G. Skrede and R. E. Wrolstad. Phenolic composition and antioxidant activities in flesh and achenes of strawberries (Fragaria ananassa). J. Agric. Food Chem., 53:4032-4040, 2005. Ahn, D., D. Putt, L. Kresty, G. D. Stoner, D. Fromm and P. F. Hollenberg. The effects of dietary ellagic acid on rat hepatic and esophageal mucosal cytochromes P450 and phase II enzymes. Carcinogenesis, 17:821-828, 1996. Ayrton, A. D., D. F. Lewis, R. Walker and C. Ioannides. Antimutagenicity of ellagic acid towards the food mutagen IQ: investigation into possible mechanisms of action. Food Chem Toxicol, 30:289-295, 1992. Bala, I., V. Bhardwaj, S. Hariharan and M. N. Kumar. Analytical methods for assay of ellagic acid and its solubility studies. J. Pharm. Biomed. Anal., 40:206-210, 2006. Barch, D. H. and C. C. Fox. Selective inhibition of methylbenzylnitrosamine-induced formation of esophageal O6-methylguanine by dietary ellagic acid in rats. Cancer Res., 48: 7088-7092, 1988. Barch, D. H. and L. M. Rundhaugen. Ellagic acid induces glutathione S-transferase Ya through activation of the antioxidant responsive element of the 5 regulatory region of the glutathione S-transferase Ya gene. Hepatology, 16:561, 1992. Barch, D. H. and L. M. Rundhaugen. Ellagic acid induces NAD(P)H:quinone reductase through activation of the antioxidant responsive element of the ratNAD(P)H:quinone reductase gene. Carcinogenesis, 15:2065-2068, 1994. Cerdaá, B., F. A. Tomas-Barberan and J. C. Espin. Metabolism of antioxidant and chemopreventive ellagitannins from strawberries, raspberries, walnuts, and oak-aged wine in humans: identification of biomarkers and individual variability. J. Agric. Food Chem., 53:227-235, 2005. Chang, R. L., M. T. Huang, A. W. Wood, C. Q. Wong, H. L. Newmark, H. Yagi, J. M. Sayer, D. M. Jerina and A. H. Conney. Effect of ellagic acid and hydroxylated flavonoids on the tumorigenicity of benzo[a]pyrene and (±)-7β,8α-dihydroxy-9α,10α-epoxy-7,8,9,10-tetrahydrobenzo(a)-pyrene on mouse skin and in the newborn mouse. Carcinogenesis, 6:1127-1133, 1985. Clifford, M. N. and A. Scalbert. Ellagitannins—nature, occurence and dietary burden. J. Sci. Food Agric., 80:1118-1125, 2000. Constantinou, A., G. D. Stoner, R. Mehta, K. Rao, C. Runyan and R. Moon. The dietary anticancer agent ellagic acid is a potent inhibitor of DNA topoisomerases in vitro. Nutr. and Cancer, 23:121-130, 1995. Daniel, E. M., A. S. Krupnick, Y.-H. Heur, J. A. Blinzler, R. W. Nims and G. D. Stoner. Extraction, stability and quantitation of ellagic acid in various fruits and nuts. J. Food Comp. Anal., 2:339-349, 1989. Feldman, K. S. and S. M. Ensel. Ellagitannin chemistry, preparative and mechanistic studies of the biomimetic oxidative coupling of galloyl easters. J. Am. Chem. Soc., 116:3357-3366, 1994. Gil, M. I., D. M. Holdcroft and A. A. Kader. Changes in strawberry anthocyanins and other polyphenols in response to carbon dioxide treatments. J. Agric. Food Chem., 45:1662-1667, 1997. Guo, C. J., J. J. Yang, J. Y. Wei, Y. F. Li, J. Xu and Y. G. Jiang. Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr. Res., 23:1719-1726, 2003. Hakkinen, S., S. Karenlampi, H. Mykkanen, I. Heinonen and A. Torronen. Ellagic acid content in berries: Influence of domestic processing and storage. Eur. Food Res. Tech., 212:75-80, 2000. Halliwell, B. and J. M. Gutteridge. Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol, 186:1-85, 1990. Hasegawa, M., M. Terauchil, Y. Kikuchi, A. Nakao, J. Okubo, T. Yoshinaga, H. Hiratsuka, M. Kobayashi and T. Hoshi. Deprotonation processes of ellagic acid in solution and solid states. Monatshefte Fur Chemie, 134:811-821, 2003. Hathway, D. E. The transformation of gallates into ellagate. Biochem. J., 67:445-450, 1957. Hertog, M. G., E. J. Feskens, P. C. Hollman, M. B. Katan and D. Kromhout. Dietary antioxidant flavonoids and risk of coronary heart disease: the Zutphen Elderly Study. Lancet, 342:1007-1011, 1993. Jurd, L. The Polyphenolic Constituents of the Pellicle of the Walnut. J. Am. Chem. Soc., 78:3445, 1956. Kamel, M. Y., N. A. Saleh and G. M. Gallic Acid Oxidation by Turnip Peroxidase. Phytochemistry, 16:521-524, 1977. Labrecque, L., S. Lamy, A. Chapus, S. Mihoubi, Y. Durocher, B. Cass, M. Bojanowski, D. Gingras and R. Beliveau. Combined inhibition of PDGF and VEGF receptors by ellagic acid, a dietary-derived phenolic compound. Carcinogenesis, 26:821-826, 2005. Li, T. M., G. W. Chen, C. C. Su, J. G. Lin, C. C. Yeh, K. C. Cheng and J. G. Chung. Ellagic acid induced p53/p21 expression, G1 arrest and apoptosis in human bladder cancer T24 cells. Anticancer Res., 25:971-979, 2005. Losso, J. N., R. R. Bansode, A. Trappey, 2nd, H. A. Bawadi and R. Truax. In vitro anti-proliferative activities of ellagic acid. J. Nutr. Biochem., 15: 672-678. 2004. Maas, J. L., S. Y. Wang and G. J. Gallette. Evaluation of strawberry cultivars for ellagic acid content. Hort. Sci., 26:66-68, 1991. Masatoshi, A., N. Hiroaki, T. Keiji, D. Hiroshi and I. Keiichiro. Agent containing an ellagic acid series compound for external application and use thereof. Patent No.:US05073545, 1991. Mayer, W., E. H. Hoffmann, N. Losch, H. Wolf, B. Wolter and G. Schilling. Dehydration reactions with gallic acid-easters. Liebigs Ann. Chem., 5:929-938, 1984. Mertens-Talcott, S. U., S. T. Talcott and S. S. Percival. Low concentrations of quercetin and ellagic acid synergistically influence proliferation, cytotoxicity and apoptosis in MOLT-4 human leukemia cells. J Nutr, 133:2669-2674, 2003. Mizusawa, K., Y. Imai, K. Yuasa, H. Koyama, N. Yamaji, S. Kataoka and T. Oguma. Process for producing ellagic acid. Patent No.:JP1989000075099, EP1990000105912, US05231193, 1989. Mullen, W., T. Yokota, M. E. Lean and A. Crozier. Analysis of ellagitannins and conjugates of ellagic acid and quercetin in raspberry fruits by LC-MSn. Phytochemistry, 64:617-624, 2003. Narayanan, B., O. Geoffroy, M. Willingham, G. Re and D. Nixon. p53/p21(WAF1/CIP1) expression and its possible role in G1 arrest and apoptosis in ellagic acid treated cancer cells. Cancer Lett., 136:215-221, 1999. Perchellet, J. P., H. U. Gali, E. M. Perchellet, D. S. Klish and A. D. Armbrust. Antitumor-promoting activities of tannic acid, ellagic acid, and several gallic acid derivatives in mouse skin. Basic Life Sci., 59:783-801, 1992. Seeram, N. P., R. Lee and D. Heber. Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice. Clin. Chim. Acta., 348:63-68, 2004. Shi, B., Q. He, K. Yao, W. Huang and Q. Li. Production of ellagic acid from degradation of valonea tannins by Aspergillus niger and Candida utilis. J. Chem. Tech. Biotech., 80:1154-1159, 2005. Shimogaki, H., Y. Tanaka, H. Tamai and M. Masuda. In vitro and in vivo evaluation of ellagic acid on melanogenesis inhibition. Int. J. Cosmet. Sci., 22:291-303, 2000. Smart, R. C., M. T. Huang, R. L. Chang, J. M. Sayer, D. M. Jerina and A. H. Conney. Disposition of the naturally-occurrung antimutagenic plant phenol, ellagic acid, and its synthetic derivatives, 3-O-decylellagic acid and 3,3'-di-O-methylellagic acid in mice. Carcinogenesis, 7:1663-1667, 1986. Stoner, G. D. and H. Mukhtar. polyphenols as cancer chemopreventive agents. J. Cell. Biochem., 22:169-180, 1995. Sun, J., Y. F. Chu, X. Z. Wu and R. H. Liu. Antioxidant and antiproliferative activities of common fruits. J. Agric. Food Chem., 50:7449-7454, 2002. Tanaka, T., H. Iwata, K. Niwa, Y. Mori and H. Mori. Inhibitory effect of ellagic acid on N-2-fluorenylacetamide-induced liver carcinogenesis in male ACI/N rats. Jpn J. Cancer Res., 79:1297-1303, 1988. Teel, R. W. Ellagic acid binding to DNA as a possible mechanism for its antimutagenic and anticarcinogenic action. Cancer Lett., 30:329-336, 1986. Teel, R. W. distribution and metabolism of ellagic acid in the mouse following intraperitoneal administration. Cancer Lett., 34:165-171, 1987. Thresiamma, K. C., J. George and R. Kuttan. Protective effect of curcumin, ellagic acid and bixin on radiation induced genotoxicity. J. Exp. Clin. Cancer Res., 17:431-434, 1998. Wang, H., G. Cao and R. Prior. Total antioxidant capacity of fruits. J. Agric. Food Chem., 44:701-705, 1996. Whitley, A. C., G. D. Stoner, M. V. Darby and T. Walle. Intestinal epithelial cell accumulation of the cancer preventive polyphenol ellagic acid--extensive binding to protein and DNA. Biochem. Pharmacol., 66:907-915, 2003. Wood, A. W., M. T. Huang, R. L. Chang, H. L. Newmark, R. E. Lehr, H. Yagi, J. M. Sayer, D. M. Jerina and A. H. Conney. Inhibition of the mutagenicity of bay-region diol epoxides of polycyclic aromatic-hydrocarbons by naturally-occuring plant phenols - exceptional activity of ellagic acid. Proc. Natl. Acad. Sci. USA, 79:5513-5517, 1982. Yoshimura, M., Y. Watanabe, K. Kasai, J. Yamakoshi and T. Koga. Inhibitory effect of an ellagic acid-rich pomegranate extract on tyrosinase activity and ultraviolet-induced pigmentation. Biosci. Biotechnol. Biochem., 69:2368-2373, 2005. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31673 | - |
dc.description.abstract | 鞣花酸(ellagic acid)是一種廣泛存在於許多漿果類水果中的多酚類化合物,已有許多研究證實鞣花酸之生理活性,包括抗氧化力、抑制癌細胞增生、誘導癌細胞凋亡等。本研究以釀製草莓酒的廢棄物---草莓粕為原料,發展簡便回收鞣花酸的製作程序,製得高濃度的鞣花酸濃縮物。在試驗中將草莓粕以1N HCl在85℃下水解3小時,原料中的鞣花單寧(ellagitannins)可水解釋出鞣花酸,此步驟使原樣品中的鞣花酸的含量由每克乾重2.3 mg增加到14.9 mg,酸水解後的樣品再經過乙酸乙酯處理除去疏水性的成分,之後將樣品以25倍體積的甲醇在室溫下震盪萃取1小時,所得萃取液加蒸餾水調成25%的甲醇水溶液,直接通入疏水性層析管柱HP-20,在流洗液中逐步增加甲醇濃度,並檢測流出液中鞣花酸的分佈情形。此製程的終產物鞣花酸純度可達80%以上,整體純化過程鞣花酸的回收率約47%。 | zh_TW |
dc.description.abstract | Ellagic acid, a phenolic compound extensively existing in many kinds of berry, has been reported to possess some physiological activities such as antioxidative, antiproliferative activities, and apoptosis inducing ability. The aim of this research was to develop a simple and rapid procedure to recover ellagic acid from the strawberry pomace of strawberry liquor manufacturing. First of all, strawberry pomace was hydrolyzed to release the free form of ellagic acid by 1N HCl at 85℃ for 3 h. This process hydrolyzed ellagitannins effectively and increased ellagic acid content from 2.3 mg/g to 14.9 mg/g. After hydrolysis, the sample was washed with ethyl acetate to remove the hydrophobic components of sample and then extracted occasionally shaking with 25-fold volume of methanol at room temperature for 1h. Three-fold volume of distilled water was added into the extract to make a 25% Methanol solution, which was then subjected into a hydrophobic HP-20 column. The separation was conducted by eluting the column with increasing the content of methanol in the mobile phase. The eluates were collected by a fraction collector. The fraction with most abundant of ellagic acid were pooled and the recovery was calculated. According to the present, concentrated ellagic acid final product could be obtained easily, and the content of ellagic acid was more than 80%, and overall, this separated process could recover 47% of ellagic acid from the raw strawberry pomace. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:17:14Z (GMT). No. of bitstreams: 1 ntu-95-R93623024-1.pdf: 1172971 bytes, checksum: 7b663f293f7d494f40904aa79b57a1c3 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 目次………………………………………………………………………1
圖次………………………………………………………………………4 表次………………………………………………………………………6 中文摘要…………………………………………………………………7 英文摘要……………………….…….........………………………8 第一章、研究動機…………………………..……………………………9 第二章、文獻整理………………………………………………………11 一、存在於自然界中的鞣花酸……………………………………11 二、鞣花酸的基本物化性質………………………………………17 三、鞣花酸的製備方法……………………………………………21 四、鞣花酸的生理活性……………………………………………23 1. 鞣花酸的抗氧化活性與抗癌活性………..…….....…………23 2. 鞣花酸的安全性、生物可利用率及分佈性…………………29 3. 鞣花酸在化妝品上做為美白成份的應用………...…………33 第三章、材料與方法……………………………………………………35 一、實驗架構………………………………………………………35 二、材料……………………………………………………………36 1.草莓粕……………………………………………….…………36 2.試藥…………………………………………….………………36 3.儀器設備……………………………………….………………37 三、實驗方法………………………………………………………38 (一)、鞣花酸測定之HPLC條件……………….………………38 (二)、酸處理對鞣花酸含量之影響…………….………………38 (三)、酸處理最適化試驗……………………….………………39 (四)、乙酸乙酯前處理………………………….………………41 (五)、鞣花酸的溶解度………………………….………………41 (六)、溶劑的萃取……………………………….………………42 (七)、萃取條件試驗…………………………….………………42 (八)、樹脂吸附試驗…………………………….………………43 (九)、疏水性管柱吸附純化試驗……………….………………44 (十)、疏水性管柱最適條件試驗……………….………………45 第四章、結果與討論……………………………………………………46 一、HPLC單一移動相之建立……………………………………46 二、酸水解的差異…………………………………………………47 三、酸水解的最適條件……………………………………………51 四、溶解度的試驗…………………………………………………51 五、溶劑選取與萃取條件…………………………………………56 六、樹脂吸附試驗…………………………………………………60 七、疏水性管柱(HP-20)吸附純化試驗…………………………63 八、疏水性管柱最適條件試驗……………………………………65 第五章、結論……………………………………………………………76 第六章、參考文獻………………………………………………………77 附錄:草莓粕樣品農藥殘留分析結果…………………………………85 | |
dc.language.iso | zh-TW | |
dc.title | 由草莓粕製備鞣花酸濃縮物方法之探討 | zh_TW |
dc.title | Development of an effective process for preparing the ellagic acid oncentrate from strawberry pomace | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 鍾玉明(Youk-Meng Choung),古國隆(Kuo-Long Ku),黃健雄(Jan-Hsiung Huang),李敏雄(Min-Hsiung Lee) | |
dc.subject.keyword | 鞣花酸,草莓,疏水性層析,多酚類化合物, | zh_TW |
dc.subject.keyword | Elagic acid,Strawberry,Hydrophobic chromatography,Polyphenolic compounds, | en |
dc.relation.page | 88 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-30 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
顯示於系所單位: | 農業化學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-95-1.pdf 目前未授權公開取用 | 1.15 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。