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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 詹東榮 | |
| dc.contributor.author | Chio-Chin Kuan | en |
| dc.contributor.author | 官照晴 | zh_TW |
| dc.date.accessioned | 2021-06-13T03:16:36Z | - |
| dc.date.available | 2011-07-31 | |
| dc.date.copyright | 2006-07-31 | |
| dc.date.issued | 2006 | |
| dc.date.submitted | 2006-07-31 | |
| dc.identifier.citation | References
No authors listed 2004, Final report of the amended safety assessment of Dioscorea Villosa (Wild Yam) root extract. Int J Toxicol 23 Suppl 2, 49-54. Bhandari, M.R., Kawabata, J., 2005, Bitterness and toxicity in wild yam (Dioscorea spp.) tubers of Nepal. Plant Foods Hum Nutr 60, 129-135. Cai, J., Liu, M., Wang, Z., Ju, Y., 2002, Apoptosis induced by dioscin in Hela cells. Biol Pharm Bull 25, 193-196. Chen, F.C., 1997, Active ingredientsand identification in common Chinese herbs. People Health Publ, China, 872 p. Choi, E.M., Hwang, J.K., 2002, Enhancement of oxidative response and cytokine production by yam mucopolysaccharide in murine peritoneal macrophage. Fitoterapia 73, 629-637. Choi, E.M., Koo, S.J., Hwang, J.K., 2004, Immune cell stimulating activity of mucopolysaccharide isolated from yam (Dioscorea batatas). J Ethnopharmacol 91, 1-6. Dermarderosian, A., Beutler, J.A., 2000, The review of natural products, 3rd ed Edition. Facts and comparsions, Missouri, 752 p. Dewick, P.M., 1997, Medicinal natural products: a biosynthetic approach. West Susses, England, 222 p. Duke, J.A., Ayensu, E.S., 1985, Medicinal plants of China. Reference Publ Inc, Michigan. Egwuagu, C.E., Yu, C.R., Zhang, M., Mahdi, R.M., Kim, S.J., Gery, I., 2002, Suppressors of cytokine signaling proteins are differentially expressed in Th1 and Th2 cells: implications for Th cell lineage commitment and maintenance. J Immunol 168, 3181-3187. Francis, G., Kerem, Z., Makkar, H.P., Becker, K., 2002, The biological action of saponins in animal systems: a review. Br J Nutr 88, 587-605. Higdon, K., Scott, A., Tucci, M., Benghuzzi, H., Tsao, A., Puckett, A., Cason, Z., Hughes, J., 2001, The use of estrogen, DHEA, and diosgenin in a sustained delivery setting as a novel treatment approach for osteoporosis in the ovariectomized adult rat model. Biomed Sci Instrum 37, 281-286. Hogan, M.B., Piktel, D., Landreth, K.S., 2000, IL-5 production by bone marrow stromal cells: implications for eosinophilia associated with asthma. J Allergy Clin Immunol 106, 329-336. Hou, R., Zhou, Q.L., Wang, B.X., Tashiro, S., Onodera, S., Ikejima, T., 2004, Diosgenin induces apoptosis in HeLa cells via activation of caspase pathway. Acta Pharmacol Sin 25, 1077-1082. Huang, K.C., 1999, The pharmacology of Chinese herbs., 2nd ed. Edition. CRC Press, New York, 512 p. Inoue, H., Kubo, M., 2004, SOCS proteins in T helper cell differentiation: implications for allergic disorders? Expert Rev Mol Med 6, 1-11. Juarez-Oropeza, M.A., Diaz-Zagoya, J.C., Rabinowitz, J.L., 1987, In vivo and in vitro studies of hypocholesterolemic effects of diosgenin in rats. Int J Biochem 19, 679-683. Kamisako, T., Ogawa, H., 2003, Regulation of biliary cholesterol secretion is associated with abcg5 and abcg8 expressions in the rats: effects of diosgenin and ethinyl estradiol. Hepatol Res 26, 348-352. Kato, A., Miura, T., Fukunaga, T., 1995, Effects of steroidal glycosides on blood glucose in normal and diabetic mice. Biol Pharm Bull 18, 167-168. Kim, M.J., Kim, H.N., Kang, K.S., Baek, N.I., Kim, D.K., Kim, Y.S., Jeon, B.H., Kim, S.H., 2004, Methanol extract of Dioscoreae Rhizoma inhibits pro-inflammatory cytokines and mediators in the synoviocytes of rheumatoid arthritis. Int Immunopharmacol 4, 1489-1497. Kurata, H., Lee, H.J., O'Garra, A., Arai, N., 1999, Ectopic expression of activated Stat6 induces the expression of Th2-specific cytokines and transcription factors in developing Th1 cells. Immunity 11, 677-688. Lee, S.C., Tsai, C.C., Chen, J.C., Lin, C.C., Hu, M.L., Lu, S., 2002a, The evaluation of reno- and hepatoprotective effects of huai-shan-yao (Rhizome Dioscoreae). Am J Chin Med 30, 609-616. Lee, S.C., Tsai, C.C., Chen, J.C., Lin, J.G., Lin, C.C., Hu, M.L., Lu, S., 2002b, Effects of 'Chinese yam' on hepato-nephrotoxicity of acetaminophen in rats. Acta Pharmacol Sin 23, 503-508. Leger, D.Y., Liagre, B., Corbiere, C., Cook-Moreau, J., Beneytout, J.L., 2004, Diosgenin induces cell cycle arrest and apoptosis in HEL cells with increase in intracellular calcium level, activation of cPLA2 and COX-2 overexpression. Int J Oncol 25, 555-562. Li, T.S., 2002, Chinese and related North American herbs: phytopharmacology and therapeutic values. CRC Press, New York., 58 p. Liagre, B., Bertrand, J., Leger, D.Y., Beneytout, J.L., 2005, Diosgenin, a plant steroid, induces apoptosis in COX-2 deficient K562 cells with activation of the p38 MAP kinase signalling and inhibition of NF-kappaB binding. Int J Mol Med 16, 1095-1101. Liagre, B., Vergne-Salle, P., Corbiere, C., Charissoux, J.L., Beneytout, J.L., 2004, Diosgenin, a plant steroid, induces apoptosis in human rheumatoid arthritis synoviocytes with cyclooxygenase-2 overexpression. Arthritis Res Ther 6, R373-383. Lin, J.Y., Lu, S., Liou, Y.L., Liou, H.L., 2006, Increased IgA and IgG serum levels using a novel yam-boxthorn noodle in a BALB/c mouse model. Food Chem Toxicol 44, 170-178. McAnuff, M.A., Harding, W.W., Omoruyi, F.O., Jacobs, H., Morrison, E.Y., Asemota, H.N., 2005a, Hypoglycemic effects of steroidal sapogenins isolated from Jamaican bitter yam, Dioscorea polygonoides. Food Chem Toxicol 43, 1667-1672. McAnuff, M.A., Omoruyi, F.O., Sotelo-Lopez, A., Asemota, H.N., 2005b, Proximate analysis and some antinutritional factor constituents in selected varieties of Jamaican yams (Dioscorea and Rajana spp.). Plant Foods Hum Nutr 60, 93-98. Moalic, S., Liagre, B., Corbiere, C., Bianchi, A., Dauca, M., Bordji, K., Beneytout, J.L., 2001, A plant steroid, diosgenin, induces apoptosis, cell cycle arrest and COX activity in osteosarcoma cells. FEBS Lett 506, 225-230. Mosmann, T.R., Sad, S., 1996, The expanding universe of T-cell subsets: Th1, Th2 and more. Immunol Today 17, 138-146. Ngoc, P.L., Gold, D.R., Tzianabos, A.O., Weiss, S.T., Celedon, J.C., 2005, Cytokines, allergy, and asthma. Curr Opin Allergy Clin Immunol 5, 161-166. Paul, W.E., Seder, R.A., 1994, Lymphocyte responses and cytokines. Cell 76, 241-251. Raju, J., Patlolla, J.M., Swamy, M.V., Rao, C.V., 2004, Diosgenin, a steroid saponin of Trigonella foenum graecum (Fenugreek), inhibits azoxymethane-induced aberrant crypt foci formation in F344 rats and induces apoptosis in HT-29 human colon cancer cells. Cancer Epidemiol Biomarkers Prev 13, 1392-1398. Rengarajan, J., Szabo, S.J., Glimcher, L.H., 2000, Transcriptional regulation of Th1/Th2 polarization. Immunol Today 21, 479-483. Rha, Y.H., Taube, C., Haczku, A., Joetham, A., Takeda, K., Duez, C., Siegel, M., Aydintug, M.K., Born, W.K., Dakhama, A., Gelfand, E.W., 2002, Effect of microbial heat shock proteins on airway inflammation and hyperresponsiveness. J Immunol 169, 5300-5307. Seki, Y., Hayashi, K., Matsumoto, A., Seki, N., Tsukada, J., Ransom, J., Naka, T., Kishimoto, T., Yoshimura, A., Kubo, M., 2002, Expression of the suppressor of cytokine signaling-5 (SOCS5) negatively regulates IL-4-dependent STAT6 activation and Th2 differentiation. Proc Natl Acad Sci U S A 99, 13003-13008. Shi, H.Q., Li, M.J., Song, A.X., Liu, X.H. 2004. RP-HPLC Determination of Diosgenin in Dioscorea opposita Thunb and D. alata L. In Chin J Pharm Anal, pp. 465-467. Shishodia, S., Aggarwal, B.B., 2006, Diosgenin inhibits osteoclastogenesis, invasion, and proliferation through the downregulation of Akt, I kappa B kinase activation and NF-kappa B-regulated gene expression. Oncogene 25, 1463-1473. Szabo, S.J., Kim, S.T., Costa, G.L., Zhang, X., Fathman, C.G., Glimcher, L.H., 2000, A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 100, 655-669. Uchida, K., Takase, H., Nomura, Y., Takeda, K., Takeuchi, N., Ishikawa, Y., 1984, Changes in biliary and fecal bile acids in mice after treatments with diosgenin and beta-sitosterol. J Lipid Res 25, 236-245. Usui, T., Nishikomori, R., Kitani, A., Strober, W., 2003, GATA-3 suppresses Th1 development by downregulation of Stat4 and not through effects on IL-12Rbeta2 chain or T-bet. Immunity 18, 415-428. Walter, D.M., McIntire, J.J., Berry, G., McKenzie, A.N., Donaldson, D.D., DeKruyff, R.H., Umetsu, D.T., 2001, Critical role for IL-13 in the development of allergen-induced airway hyperreactivity. J Immunol 167, 4668-4675. Wanasundera, J.P., Ravindran, G., 1994, Nutritional assessment of yam (Dioscorea alata) tubers. Plant Foods Hum Nutr 46, 33-39. Wang, L.J., Wang, Y., Chen, S.W., Ma, J.S., Fu, Q., Wang, B.X., 2002, [The antitumor activity of Diosgenin in vivo and in vitro]. Zhongguo Zhong Yao Za Zhi 27, 777-779. Yamada, T., Hoshino, M., Hayakawa, T., Ohhara, H., Yamada, H., Nakazawa, T., Inagaki, T., Iida, M., Ogasawara, T., Uchida, A., Hasegawa, C., Murasaki, G., Miyaji, M., Hirata, A., Takeuchi, T., 1997, Dietary diosgenin attenuates subacute intestinal inflammation associated with indomethacin in rats. Am J Physiol 273, G355-364. Yamaguchi, A., Tazuma, S., Ochi, H., Chayama, K., 2003, Choleretic action of diosgenin is based upon the increases in canalicular membrane fluidity and transporter activity mediating bile acid independent bile flow. Hepatol Res 25, 287-295. Yamamoto, K., Yamaguchi, M., Miyasaka, N., Miura, O., 2003, SOCS-3 inhibits IL-12-induced STAT4 activation by binding through its SH2 domain to the STAT4 docking site in the IL-12 receptor beta2 subunit. Biochem Biophys Res Commun 310, 1188-1193. Yen, M.L., Su, J.L., Chien, C.L., Tseng, K.W., Yang, C.Y., Chen, W.F., Chang, C.C., Kuo, M.L., 2005, Diosgenin induces hypoxia-inducible factor-1 activation and angiogenesis through estrogen receptor-related phosphatidylinositol 3-kinase/Akt and p38 mitogen-activated protein kinase pathways in osteoblasts. Mol Pharmacol 68, 1061-1073. Yin, J., Tezuka, Y., Kouda, K., Le Tran, Q., Miyahara, T., Chen, Y., Kadota, S., 2004, In vivo antiosteoporotic activity of a fraction of Dioscorea spongiosa and its constituent, 22-O-methylprotodioscin. Planta Med 70, 220-226. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31646 | - |
| dc.description.abstract | 山藥在傳統中醫學上被認為具有減輕疲勞、安定神經、止咳、化痰、抗發炎、抗氣喘、治療腹瀉等功效，但大多缺乏科學證實。在亞洲國家，山藥被視為滋養強身增進免疫力的健康食品，廣被一般民眾食用。Diosgenin（DIO）為山藥含量最豐富的皂苷元，並且具有多種的生物活性，如降血糖、抗腫瘤、增進膽汁排放等，本研究主要目的在探討DIO對卵白蛋白敏感化及氣霧刺激之BALB/c鼠之全身系統性抗體生成反應及脾臟細胞表現細胞激素的作用，並進一步探討其對Th細胞分化的可能影響。DIO的給予為每日一次管餵200或400 毫克/公斤體重（DIO 200和DIO 400），共給予34天，餵藥期間以卵白蛋白(ovalbumin)腹腔注射敏感化兩次、氣霧刺激三次，血清抗體以ELISA檢測共兩次，第一次是在小鼠第一次卵白蛋白敏感化兩週後，第二次則在最後ㄧ次卵白蛋白氣霧刺激48小時之後。第一次的血清結果顯示，DIO 200降低卵白蛋白專一性抗體IgE含量，DIO 200及DIO 400降低整體IgE的生成；而第二次的血清結果顯示，DIO 200及DIO 400對卵白蛋白專一性的抗體IgG2a生成有明顯的促進作用。分析脾臟細胞的細胞激素表現，Th1細胞激素IFN-γ的蛋白質分泌和mRNA表現在DIO餵食的小鼠皆明顯高於對照組，進一步以RT-PCR去偵測調控Th1分化的轉錄因子T-bet的mRNA表現，也有上升的趨勢。然而DIO對Th2細胞激素IL-4的分泌和mRNA的表現並無作用，偵測上游Th2的轉錄因子GATA-3及細胞激素抑制蛋白SOCS3的mRNA表現，也未受到DIO的影響。在肺臟組織中的IL-4 mRNA的表現、病理組織切片炎症細胞浸潤情形及黏液的生成也都顯示DIO不具有降低呼吸道局部炎症反應的作用。
综合上述結果，DIO對早期的抗體IgE生成有所調控，但在晚期的卵白蛋白加強免疫的抗體IgE和Th2分化、細胞激素相關的調控上並無活性。然而DIO具有增加抗原專一性抗體IgG2a的生成和IFN-γ表現的作用，DIO並具有促進Th1細胞分化的活性，使Th1/Th2平衡朝向Th1的趨勢，本研究的結果指出山藥的皂苷元成分在活體動物模式具有增進某些免疫反應的活性。 | zh_TW |
| dc.description.abstract | Chinese yam is a commonly used herb in Taiwan and several other Asian countries. The most abundant steroidal sapogenin contained in the root of Chinese yam is diosgenin (DIO), which has been shown to exhibit a number of biological activities. The objective of the present study was to investigate the effect of DIO on antibody production and T-cell reactivity in BALB/c mice sensitized with ovalbumin (OVA) and subsequently challenged with aerosolized OVA. Mice were administered with DIO (200 or 400 mg/kg) by gavage daily for 34 days. Serum samples were obtained 2 weeks after each OVA sensitization, and mice were sacrificed 2 days post the OVA challenge. Diosgenin treatment attenuated the production of OVA-specific IgE and total IgE in serum obtained after the 1st Ova sensitization, but significantly enhanced IgG2a in serum after the 2nd sensitization. In parallel with the enhancement of anti-OVA IgG2a, the expression of the Th1 cytokine IFN-gamma and T-bet transcription factor in splenocyes isolated from DIO-treated mice was markedly elevated, whereas the expression of the Th2 cytokine IL-4 and GATA-3 transcription factor was unaffected. Accordantly, DIO treatment did not affect the steady state mRNA expression of IL-4 in the lungs of mice sensitized and challenged with OVA. Moreover, the production of mucin and infiltration of inflammatory cells in the lungs of OVA-sensitized and challenged mice were not affected by DIO treatment. Taken together, the data suggest that exposure of mice to DIO results in modulation of the immune response toward Th1 direction in response to OVA sensitization, whereas Th2 cell-derived immunity and allergic airway reactions were relatively unaltered. The present study is the first report to show the immunomodulatory activity of DIO in vivo. Our data provide the evidence that intake of DIO, the major sapogenin contained in Chinese yam, may up-regulate certain aspects of immunity due to the enhancement of IFN-gamma expression and Th1 differentiation. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T03:16:36Z (GMT). No. of bitstreams: 1 ntu-95-R93629013-1.pdf: 811843 bytes, checksum: 9a64c1ccdd482b0026bfab3f3a8c3bf1 (MD5) Previous issue date: 2006 | en |
| dc.description.tableofcontents | 中文摘要 i
Abstract iii Contents v List of Tables viii Abbreviations ix Introduction 1 I. Chinese yam background 1 A. Major constituents of yams 1 B. The therapeutic effects of Chinese yam in traditional Chinese medicine 2 C. Saponins 3 II. The biological effects of yam ingredients 8 A. Hypocholesterolemic effect 8 B. Prevention of osteoporosis 8 C. Hypoglycemic effect 9 D. Anti-tumor effect 9 E. Immunomadulation 10 F. Other effects 10 G. The absorption and distribution of diosgenin in vivo 11 III. T helper (Th) cell differentiation 11 A. Regulation of Th cell activation and differentiation by suppressors of cytokine signaling (SOCS) proteins 13 B. Th2 cytokine and allergic airway response 15 IV. Objective of this study 16 Materials and methods 18 I. Animals 18 II. Protocol of DIO administration, and OVA sensitization and challenge 18 III. Measurement of serum OVA-specific IgG1, IgG2a, IgM 19 IV. Measurement of OVA-specific IgE and total IgE 21 V. Culture of splenocytes 22 VI. Measurement of cytokines 22 VII. Necropsy and pulmonary tissue preparation 23 VIII. RNA isolation 24 IX. Reverse transcriptase-polymerase chain reaction (RT-PCR) 25 X. Statistical analysis 27 Experimental results 28 I. No effect on the body weight of BALB/c mice by diosgenin (DIO) treatment 28 II. Effects of DIO on OVA-specific serum immunoglobulins (Igs) and total IgE production in OVA-sensitized mice 28 III. Effects of DIO on IFN-γ and IL-4 secretion by splenocytes isolated from OVA-sensitized mice 29 IV. Effects of DIO on IFN-γ and IL-4 mRNA expression by splenocytes isolated from OVA-sensitized mice 31 V. Effects of DIO on T-bet and GATA-3 mRNA expression by splenocytes isolated from OVA-sensitized mice 31 VI. No effect by DIO treatment on SOCS3 mRNA expression by splenocytes 32 VII. No effect by DIO treatment on OVA-induced IL-4 expression and inflammatory responses in the lungs of OVA-sensitized mice 33 Discussion 49 References 54 | |
| dc.language.iso | en | |
| dc.subject | 基因表現 | zh_TW |
| dc.subject | 山藥 | zh_TW |
| dc.subject | 植物固醇 | zh_TW |
| dc.subject | 免疫調節 | zh_TW |
| dc.subject | T細胞 | zh_TW |
| dc.subject | 細胞激素 | zh_TW |
| dc.subject | Chinese yam | en |
| dc.subject | gene expression | en |
| dc.subject | cytokine | en |
| dc.subject | T cell | en |
| dc.subject | steroidal sapogenin | en |
| dc.subject | diosgenin | en |
| dc.title | 類固醇皂苷元diosgenin促進卵白蛋白免疫小鼠表現抗原專一性IgG2a抗體和伽馬干擾素 | zh_TW |
| dc.title | Diosgenin, a steroidal sapogenin, enhances antigen-specific IgG2a and interferon-γ expression in ovalbumin-sensitized BALB/c mice | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 94-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 龐飛,劉興華,戚謹文 | |
| dc.subject.keyword | 山藥,植物固醇,免疫調節,T細胞,細胞激素,基因表現, | zh_TW |
| dc.subject.keyword | Chinese yam,diosgenin,steroidal sapogenin,T cell,cytokine,gene expression, | en |
| dc.relation.page | 58 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2006-07-31 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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