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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻(Tai-Horng Young) | |
dc.contributor.author | Chih-Huang Hung | en |
dc.contributor.author | 洪智煌 | zh_TW |
dc.date.accessioned | 2021-06-13T03:16:25Z | - |
dc.date.available | 2006-07-31 | |
dc.date.copyright | 2006-07-31 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-31 | |
dc.identifier.citation | chapter 1:
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Assessment and modeling of poly (vinyl alcohol) bioartificial pancreas in vivo. Biomaterials 2002; 23: 3495-3301. 12. Young TH, Yao NK, Chuang WY, Chen LW. Use of a diffusion model for assessing the performance of poly (vinyl alcohol) bioartificial pancreases. J Biomed Mater Res 1998; 40: 385-391. 13. Young TH, Hu WW. Covalent bonding of lysine to EVAL membrane surface to improve survival of cultured cerebellar granule neurons, Biomaterials 2003; 24: 1477-1486. 14. Johe KK, Hazel TG, Muller T Dugich-Djordjevic MM, McKay RDG. Single factors direct the differentiation of stem cells from the fetal and adult central nervous system. Genes Dev. 1996; 10: 3129-3140. 15. Bottenstein JE, Sato GH. Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc Natl Acad Sci USA 1979; 76: 514-517. 16. Tseng YC, Park K. Synthesis of photoreactive poly (ethylene glycol) and its application to the prevention of surface-induced platelet activation. 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Nature 1994;372:263-5. 5. Johe KK, Hazel TG, Muller T, Dugich-Djordjevic MM, McKay RD. Single factors direct the differentiation of stem cells from the fetal and adult central nervous system. Genes Dev 1996;10:3129-40. 6. Tsai RY, McKay RD. Cell contact regulates fate choice by cortical stem cells. J Neurosci 2000;20:3725-35. 7. Ciccolini F, Svendsen CN. Fibroblast growth factor 2 (FGF-2) promotes acquisition of epidermal growth factor (EGF) responsiveness in mouth striatal precursor cells: Identification of neural precursors responding to both EGF and FGF-2. J Neurosci 1998;18:7869-80. 8. Arsenijevic Y, Weiss S, Schneider B, Aebischer P. Insulin-like growth factor-1 is necessary for neural stem cell proliferation and demonstrates distinct actions of epidermal growth factor and fibroblast growth factor-2. J Neurosci 2001;21:7194-202. 9. Shimazaki T, Shingo T, Weiss S. The ciliary neurotrophic factor/leukemia inhibitory factor/gp 130 receptor complex operates in the maintenance of mammalian forebrain neural stem cells. J Neurosci 2001;21:7642-53. 10. Young TH, Hung CH. Behavior of embryonic rat cerebral cortical stem cells on the PVA and EVAL substrates. Biomaterials 2005;26:4291-99. 11. Schmidt CE, Leach JB. Neural tissue engineering: strategies for repair and regeneration. Annu Rev Biomed Eng 1993;5:293-347. 12. Geller HM, Fawcett JW. Building a bridge: engineering spinal cord repair. Exp Neurol 2002;174(2):125-36. 13. Yavin E, Yavin Z. Attachment and culture of dissociated cells from rat embryo hemispheres on poly-D-lysine coated surface. J Cell Biol 1974;62:540-6. 14. Banker GA, Cowan WM. Rat hippocampal neurons in dispersed cell culture. Brain Res 1977;126:397-425. 15. Mattson MP, Dou P, Kater SB. Outgrowth-regulating actions of glutamate in isolated hippocampal pyramidal neurons. J Neurosci 1988;8:2087-2100. 16. Brewer G, Cotman C. 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Microtubule-Associated Protein 2 Appears in Axons of Cultured Dorsal Root Ganglia and Spinal Cord Neurons After Rotavirus Infection. J Neurosci Res 1993;36:173-82. 27. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res 1972;43:429-35. 28. Sommer I, Schachner M. Monoclonal antibody (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system. Dev Biol 1981;83:311-27. 29. Duittoz AH, Hevor T. Primary culture of neural precursors from the ovine central nervous system (CNS). J Neurosci Methods 2001;107:131-40. 30. Diez-Guerra FJ, Avila J. An increase in phosphorylation of microtubule associated protein 2 accompanies dendrite extension during the differentiation of cultured hippocampal neurons. Eur J Biochem 1995;227:68-77. 31. Shetty AK, Turner DA. In vitro survival and differentiation of neurons derived from epidermal growth factor-responsive postnatal hippocampal stem cells: inducing effects of brain-derived neurotrophic factor. J Neurobiol 1998;35:395-425. 32. Reynolds BA, Tetzlaff W, Weiss S. A multipotent EGF-responsive striatal embryonic progenitor cells produces neurons and astrocytes. J Neurosci 1992;12:4565-74. 33. Barakat I, Sensenbrenner M, Vincendon G. The importance of cell contact for the proliferation of neuroblasts in culture and its stimulation by meningeal extract. Neurochem Res 1982;7:287-300. 34. Albrecht-Buehler G. A long-range attraction between aggregating 3T3 cells mediated by bear-infrared light scattering. Proc Natl Acad Sci USA 2005;102:5050-5. 35. Young TH, Huang JH, Hung SH, Hsu JP. The role of cell density in the survival of cultured cerebellar granule neurons. J Biomed Mater Res 2000;52:748-53. 36. Kilpatrick TJ, Bartlett PF. Cloning and growth of multipotential neural precursors: requirements for proliferation and differentiation. Neuron 1993;10:255-65. 37. Hulspas R, Tiarks C, Reilly J, Hsieh CC, Recht L, Quesenberry PJ. In vitro cell density-dependent clonal growth of EGF-responsive murine neural progenitor cells under serum-free conditions. Exp Neurol 1997;148:147-56. 38. Noble M, Fok-Seang J, Cohen J. Glia are a unique substrate for the in vitro growth of central nervous system neurons. J Neurosci 1984;4(7):1892-903. 39. Varon S. Neurons and glial in neuronal cultures. Exp Neurol 1975;48:93-134. 40. Gasser UE, Hatten ME. Neuron-glia interactions of rat hippocampal cells in vitro: glial guided neuronal migration and neuronal regulation of glial differentiation. J Neurosci 1990;10(4):1276-85. 41. Costa S, et al., Astroglial permissivity for neuritic outgrowth in neuron-astrocyte coculture depends on regulation of laminin bioavailability. Glia 2002;37:105-13. chapter 4: 1. Mckay R. Stem cells in the central nervous system. Science 1997;276:66-70. 2. Reynolds BA, Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 1992;255:1707-10. 3. Davis AA, Temple S. A self-renewing multipotential stem cell in embryonic rat cerebral cortex. Nature 1994;372:263-5. 4. Cameron HA, Hazel TG, Mckay RDG. Regulation of neurogenesis by growth factors and neurotransmitters. J Neurobiol 1998;36:287-306. 5. Cattaneo E, McKay R. Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor. Nature 1990;347:762-5. 6. Ciccolini F, Svendsen CN. Fibroblast growth factor 2 (FGF-2) promotes acquisition of epidermal growth factor (EGF) responsiveness in mouth striatal precursor cells: Identification of neural precursors responding to both EGF and FGF-2. J Neurosci 1998;18:7869-80. 7. Arsenijevic Y, Weiss S, Schneider B, Aebischer P. Insulin-like growth factor-1 is necessary for neural stem cell proliferation and demonstrates distinct actions of epidermal growth factor and fibroblast growth factor-2. J Neurosci 2001;21:7194-202. 8. Johe KK, Hazel TG, Muller T, Dugich-Djordjevic MM, McKay RD. Single factors direct the differentiation of stem cells from the fetal and adult central nervous system. Genes Dev 1996;10:3129-40. 9. Tsai RY, McKay RD. Cell contact regulates fate choice by cortical stem cells. J Neurosci 2000;20:3725-35. 10. Young TH, Hung CH. Behavior of embryonic rat cerebral cortical stem cells on the PVA and EVAL substrates. Biomaterials 2005;26:4291-99. 11. Yavin E, Yavin Z. Attachment and culture of dissociated cells from rat embryo hemispheres on poly-D-lysine coated surface. J Cell Biol 1974;62:540-6. 12. Mattson MP, Dou P, Kater SB. Outgrowth-regulating actions of glutamate in isolated hippocampal pyramidal neurons. J Neurosci 1988;8:2087-2100. 13. Young TH, Hu WW. Covalent bonding of lysine to EVAL membrane surface to improve survival of cultured cerebellar granule neurons. Biomaterials 2003;24:1477-86. 14. Varon S. The culture of chick embryo dorsal root ganglionic cells on polylysine-coated plastic. Neurochem Res 1979;4:155-73. 15. Lochter A, Vaughan L, Kaplony A, Prochiabtz A, Schachner M, Faissner A. J1/tenascin-C in substrate-bound and soluble form displays contrary effects on neurite outgrowth. J Cell Biol 1991;113:1159-71. 16. Young TH, Yao CH, Sun JS, Lai CP, Chen LW. The effect of morphology variety of EVAL membranes on the behavior of myoblasts in vitro. Biomaterials 1998;19:717-24. 17. Bottenstein JE, Sato GH. Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc Natl Acad Sci USA 1979;76:514-7. 18. Gritti A, Parati EA, Cova L, Frolichsthal P, Galli R, Wanke E, Faravelli L, Morassutti DJ, Roisen F, Nickel DD, Vescovi AL. 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Mosmann T. Rapid colorimetric assay for cellular growth and survival: application of proliferation and cytotoxicity assays. J Immunol Meth 1983;65:55-63. 25. Deckwerth TL, Johnson Jr EM. Temporal analysis of events associated with programmed cell death (apoptosis) of sympathetic neurons deprived of nerve growth factor. J Cell Biol 1993;123:1207-22. 26. Shetty AK, Turner DA. In vitro survival and differentiation of neurons derived from epidermal growth factor-responsive postnatal hippocampal stem cells: inducing effects of brain-derived neurotrophic factor. J Neurobiol 1998;35:395-425. 27. Reynolds BA, Tetzlaff W, Weiss S. A multipotent EGF-responsive striatal embryonic progenitor cells produces neurons and astrocytes. J Neurosci 1992;12:4565-74. 28. Wang JH, Hung CH, Young TH. Proliferation and differentiation of neural stem cells on lysine-alanine sequential polymer substrates. Biomaterials 2006;27:3441-50. 29. Bixby JL, Harris WA. Molecular mechanisms of axons growth and guidance. 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Johe KK, Hazel TG, Muller T, Dugich-Djordjevic MM, McKay RD. Single factors direct the differentiation of stem cells from the fetal and adult central nervous system. Genes Dev 1996;10:3129-40. 2. Tsai RY, McKay RD. Cell contact regulates fate choice by cortical stem cells. J Neurosci 2000;20:3725-35. 3. Cameron HA, Hazel TG, Mckay RDG. Regulation of neurogenesis by growth factors and neurotransmitters. J Neurobiol 1998;36:287-306. 4. Reynolds BA, Weiss S. Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system. Science 1992;255:1707-10. 5. Ciccolini F, Svendsen CN. Fibroblast growth factor 2 (FGF-2) promotes acquisition of epidermal growth factor (EGF) responsiveness in mouth striatal precursor cells: Identification of neural precursors responding to both EGF and FGF-2. J Neurosci 1998;18:7869-80. 6. Arsenijevic Y, Weiss S, Schneider B, Aebischer P. Insulin-like growth factor-1 is necessary for neural stem cell proliferation and demonstrates distinct actions of epidermal growth factor and fibroblast growth factor-2. J Neurosci 2001;21:7194-202. 7. Shimazaki T, Shingo T, Weiss S. The ciliary neurotrophic factor/leukemia inhibitory factor/gp 130 receptor complex operates in the maintenance of mammalian forebrain neural stem cells. J Neurosci 2001;21:7642-53. 8. Schmidt CE, Leach JB. Neural tissue engineering: strategies for repair and regeneration. Annu Rev Biomed Eng 1993;5:293-347. 9. Young TH, Hung CH. Behavior of embryonic rat cerebral cortical stem cells on the PVA and EVAL substrates. Biomaterials 2005;26:4291-99. 10. Noble M, Fok-Seang J, Cohen J. Glia are a unique substrate for the in vitro growth of central nervous system neurons. J Neurosci 1984;4(7):1892-903. 11. Young TH, Yao CH, Sun JS, Lai CP, Chen LW. The effect of morphology variety of EVAL membranes on the behavior of myoblasts in vitro. Biomaterials 1998; 19:717-724. 12. Bottenstein JE, Sato GH. Growth of a rat neuroblastoma cell line in serum-free supplemented medium. Proc Natl Acad Sci USA 1979;76:514-7 13. Wong G, Goldshmit Y, Turnley AM. Interferon-γ but not TNFα promotes neuronal differentiation and neurite outgrowth of murine adult neural stem cells. Exp Neurol 2004;187:171-7. 14. Chiang YH, Silani V, Zhou FC. Morphological differentiation of astroglial progenitor cells from EGF-responsive neurospheres in response to fetal calf serum, basic fibroblast growth factor, and retinal. Cell transplant 1996;5(2):179-89. 15. Gritti A, Parati EA, Cova L, Frolichsthal P, Galli R, Wanke E, Faravelli L, Morassutti DJ, Roisen F, Nickel DD, Vescovi AL. Multipotential stem cells from the adult mouse brain proliferate and self-renew in response to basic fibroblast growth factor. J Neurosci 1996;16:1091-100. 16. Weclewicz K, Svensson L, Billger M, Holmberg K, Wallin M, Kristensson K. Microtubule-Associated Protein 2 Appears in Axons of Cultured Dorsal Root Ganglia and Spinal Cord Neurons After Rotavirus Infection. J Neurosci Res 1993;36:173-82. 17. Dráberová E, Lukás Z, Ivanyi D, Viklický V, Dráber P. Expression of class III beta-tubulin in normal and neoplastic human tissue. Histochem Cell Biol 1998;109:231-9. 18. Bignami A, Eng LF, Dahl D, Uyeda CT. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res 1972;43:429-35. 19. Sommer I, Schachner M. Monoclonal antibody (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system. Dev Biol 1981;83:311-27. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31638 | - |
dc.description.abstract | 本研究探討大鼠胚胎之大腦皮層神經幹細胞在高分子聚乙烯乙烯醇、聚乙烯醇以及離氨酸-丙氨酸序列共聚物基材上行為之調控。在體外培養時,神經幹細胞的行為受到下列三種因素所調控: (1) 培養基中的可溶性因子,(2) 細胞與細胞之間的交互影響,(3) 培養的基材,因此,藉由改變這三者之間的交互作用來控制神經幹細胞在生醫基材上的行為與生長。
第一章介紹幹細胞、中樞神經系統、中樞神經系統之神經幹細胞、影響神經幹細胞生長行為的因素、神經幹細胞的免疫化學分析、生醫基材、以及神經幹細胞的應用。 第二章探討神經幹細胞在沒有血清的條件下,其在高分子聚乙烯乙烯醇以及聚乙烯醇薄膜基材上行為之調控。在纖維母細胞生長因子的作用下,神經幹細胞會因細胞與細胞之間交互作用的強度,而改變生長方式。當以單顆神經幹細胞培養在聚乙烯乙烯醇基材上時,也就是細胞與細胞之間的交互作用較小時,細胞會貼附於基材上,但不分化,根據染色結果顯示,仍保有高比例的神經幹細胞。另外,當以低密度之神經幹細胞團培養時,神經幹細胞團會貼附至聚乙烯乙烯醇基材上並被誘導分化;假如繼續提高神經幹細胞的密度時,也就是提高細胞與細胞之間的引力,此時神經幹細胞並不會貼附於基材上,反而懸浮於基材上並被誘導增生。另外,結果也顯示在此條件下,神經幹細胞無法存活於聚乙烯醇薄膜基材上。 第三章則探討神經幹細胞團在以丙氨酸-離氨酸序列共聚物為基材上行為之調控,結果顯示在沒有血清的條件下,神經幹細胞團會因為培養時密度的不同,而採用不同的方式與鄰近的細胞聯繫。另外,當血清加入到此系統中後,因為血清對細胞的效應大過於基材對細胞的效應或細胞與細胞之間的效應,此時神經幹細胞已呈現不同的生長方式。 第四章則是探討當血清直接加至培養基中或以塗佈的方式塗佈於聚乙烯乙烯醇與聚乙烯醇薄膜基材對神經幹細胞行為的影響。結果顯示培養基中的血清與塗佈的血清對於神經幹細胞的分化各有不同的影響,然而此時所使用的基材會影響神經幹細胞分化的細胞類型。 第五章則是探討神經生長因子對於培養在聚乙烯乙烯醇基材上之神經幹細胞團的影響,結果顯示,只有在沒有血清條件下,且在神經生長因子的作用下,神經束才會形成束狀生長。 第六章總結本研究的貢獻,藉由調控神經幹細胞生長的微環境,以此控制神經幹細胞在生醫材料上的行為包括誘導增生、分化。 | zh_TW |
dc.description.abstract | In this study, the behavior of embryonic rat cortical neural stem cells on biomedical polymer substrates, poly-(ethylene-co-vinyl alcohol) (EVAL), polyvinyl alcohol (PVA), and lysine-alanine sequential (LAS) were explored. However, the behavior of neural stem cells in vitro are mediated by three major factors: (1) soluble factors in the medium, (2) cell-cell interactions, and (3) culture substrates. Hence, by means of altering the interactions of these three factors, the behavior of cortical neural stem cells was manipulated on these substrates.
Chapter one introduces the backgrounds of stem cells, central nervous system (CNS), neural stem cells from CNS, the factors regulated the behavior of neural stem cells, immunocytochemistry for neural stem cells, biomedical polymer substrates, and application of neural stem cells. Chapter two investigates the behavior of neural stem cells on PVA and EVAL membranes in the presence of the mitogenic effect of basic fibroblast growth factor (bFGF) in the serum-free medium. It was found that EVAL and PVA membranes exerted different influences on the fate of neural stem cells. The behavior of neural stem cells on the EVAL membrane was independent of cell density at the single-cell level. Conversely, the development of cell clusters was in a density-dependent manner on the EVAL membrane. Neurospheres continuously proliferated under high-density culture condition, but differentiated into neurons and astrocytes under low-density culture condition. Therefore, it is reasonable to assume that biomaterials may stimulate or inhibit the proliferation and differentiation of neural stem cells. Chapter three explores the phenotypic potential of cortical neural stem cells by inducing differentiation on LAS polymer substrates at neurosphere-level. The results provided evidences that LAS could improve better process growth of neurospheres than poly-D-lysine could. The results also suggested that the behavior, proliferation, and differentiation of neural stem cells on LAS substrates is responsible for the effects of seeding density and serum proteins. It was found that forming-neurospheres cells cultured on LAS substrates adopted different strategies to communicate with adjacent neurospheres Chapter four investigates the influence of fetal bovine serum (FBS) adsorbed to EVAL and PVA substrates (coated FBS) and fetal bovine serum present in the culture medium (soluble FBS) on the behavior of cortical neural stem cells at neurosphere level. The results of this study suggest that besides substrates, coated and soluble serum proteins have their unique effect on the morphological differentiation and fate determination of neural stem cells. Chapter five explores the effects of cell-cell and cell-substrate interactions on developmental potential of neural stem cells at neurosphere level in the presence of nerve growth factors (NGF) on tissue culture polystyrene (TCPS), poly-D-lysine (PDL), and EVAL substrates. The results also suggested that, in combination with NGF, EVAL substrates could induce process fasciculation of differentiated forming-neurosphere cells under serum free conditions. The results also provided evidence that EVAL can be a potent controller of cell interactions involving cell-cell and cell-substrate contacts. We proposed that, in combination with nerve growth factors, EVAL substrates could induce neurite fasciculation of embryonic rat cortical neural stem cells under serum free conditions. Chapter six concludes the contribution of this study and suggested that the present study provides evidence that the plasticity of multipotential neural stem cells is versatile and is dependent on the complex environmental conditions. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T03:16:25Z (GMT). No. of bitstreams: 1 ntu-95-D91548011-1.pdf: 16870352 bytes, checksum: 36875f33902f70781a39f2fd51be51d7 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | LIST OF FIGURES IV
摘要 IX ABSTRACT XI CHAPTER 1 INTRODUCTION 1 1.1. STEM CELLS 1 1.2. THE CENTRAL NERVOUS SYSTEM 1 1.3. NEURAL STEM CELLS FROM CNS 2 1.4. THE FACTORS REGULATED THE BEHAVIOR OF NEURAL STEM CELLS 5 1.5. IMMUNOCYTOCHEMISTRY FOR NEURAL STEM CELLS 7 1.6. BIOMEDICAL POLYMER SUBSTRATES 7 1.7. APPLICATION OF NEURAL STEM CELLS 8 1.8 REFERENCES 10 CHAPTER 2 THE BEHAVIOR OF EMBRYONIC RAT CEREBRAL CORTICAL NEURAL STEM CELLS ON THE PVA AND EVAL MEMBRANES 13 ABSTRACT 13 2.1. INTRODUCTION 15 2.2. EXPERIMENTAL MATERIALS AND METHODS 17 2.2.1. Preparation of Substrates 17 2.2.2. Culture of Primary Neural Stem Cells 17 2.2.3. Immunocytochemistry 18 2.3. RESULTS 20 2.3.1. Cell Characterization 20 2.3.2. Influence of PVA on the Behaviour of Neural Stem Cells 20 2.3.3. Influence of EVAL on the Behaviour of Neural Stem Cells 21 2.4. DISCUSSION 24 2.5. CONCLUSION 28 2.6 REFERENCES 29 CHAPTER 3 PROLIFERATION AND DIFFERENTIATION OF NEURAL STEM CELLS ON LYSINE-ALANINE SEQUENTIAL SUBSTRATES 32 ABSTRACT 32 3.1. INTRODUCTION 34 3.2. EXPERIMENTAL MATERIALS AND METHODS 36 3.2.1. Preparation of LAS and PDL Substrates 36 3.2.2. Isolation and Culture of Cortical Neural Stem Cells 36 3.2.3. Quantification of Process Growth 37 3.2.4. MTT Assay 38 3.2.5. Immunocytochemistry 38 3.3. RESULTS 40 3.3.1. BEHAVIOR OF NEUROSPHERES ON LAS SUBSTRATES AT LOW DENSITY UNDER SERUM-FREE CONDITIONS 40 3.3.2. Behavior of Neurospheres on LAS Substrates at High Density under Serum-Free Conditions 42 3.3.3. Behavior of Neurospheres on LAS Substrates at Low Density in the Presence of Serum 43 3.3.4. MTT assay 44 3.4. DISCUSSION 46 3.5. CONCLUSION 50 3.6. REFERENCES 51 CHAPTER 4 DIFFERENCES IN THE EFFECT ON NEURAL STEM CELLS OF FETAL BOVINE SERUM IN SUBSTRATE-COATED AND SOLUBLE FORM 56 ABSTRACT 56 4.1. INTRODUCTION 58 4.2. EXPERIMENTAL MATERIALS AND METHODS 60 4.2.1. Preparation of EVAL and PVA Membranes 60 4.2.2. Isolation and Culture of Cortical Neural Stem Cells 60 4.2.3. Preparation of Fetal Bovine Serum-Coated Substrates 61 4.2.4. Immunocytochemistry 61 4.2.5. Quantification of Cell Migration 62 4.2.5. MTT Assay 63 4.3. RESULTS 64 4.3.1. Behavior of Neurospheres on EVAL and PVA Substrates under Serum-Free Conditions 64 4.3.2. Behavior of Neurospheres on FBS-Coated EVAL and PVA Substrates in the Serum-Free Medium 64 4.3.3. Behavior of Neurospheres on EVAL and PVA Substrates in the Medium Containing 10% FBS 66 4.3.4. Behavior of Neurospheres on FBS-Coated EVAL and PVA Substrates in the Medium Containing 10% FBS 67 4.4.5. MTT Assay 68 4.4 DISCUSSION 70 4.5. CONCLUSION 75 4.6. REFERENCES 76 CHAPETER 5 INDUCTION OF NEURONAL DIFFERENTIATION OF NEUROSPHERES BY NERVE GROWTH FACTORS 80 ABSTRACT 80 5.1. INTRODUCTION 82 5.2. EXPERIMENTAL MATERIALS AND METHODS 84 5.2.1. Fabrication of EVAL Membranes 84 5.2.2. Isolation and Culture of Cortical Neural Stem Cells 84 5.2.3. Quantification of Process Length and Breadth 85 5.2.4. Immunocytochemistry 86 5.3. RESULTS AND DISCUSSION 88 5.3.1. The Behavior of Neurospheres on TCPS with or without the Addition of NGF under Serum-Free Conditions 88 5.3.2. The Behavior of Neurospheres on TCPS with or without the Addition of NGF in the Medium Containing 10% FBS 89 5.3.3. The Behavior of Neurospheres on PDL Substrates with or without the Addition of NGF under Serum-Free Conditions 90 5.3.4. The Behavior of Neurospheres on PDL Substrates with or without the Addition of NGF in the Medium Containing 10% FBS 91 5.3.5. The Behavior of Neurospheres on EVAL Substrates with or without the Addition of NGF under Serum-Free Conditions or in the Medium Containing 10% FBS 92 5.4. CONCLUSION 95 5.5. REFERENCES 96 CHAPTER 6 CONCLUSION 99 | |
dc.language.iso | en | |
dc.title | 大鼠胚胎之大腦皮層神經幹細胞在高分子基材上行為之探討 | zh_TW |
dc.title | The behavior of embryonic rat cerebral cortical neural stem cells on the polymer substrates | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 何弘能(Hong-Nerng Ho),薛敬和(GING-HO HSIUE),邱英明(Ing-Ming Chiu),謝松蒼(Sung-Tsang Hsieh),王盈錦(Yng-Jiin Wang) | |
dc.subject.keyword | 神,經幹細胞,聚乙烯乙烯醇,聚乙烯醇,離,氨酸-丙氨酸序列,共聚物,胎牛血清,神,經生長因子, | zh_TW |
dc.subject.keyword | neural stem cells,poly-(ethylene-co-vinyl alcohol),polyvinyl alcohol,lysine-alanine sequential,fetal bovine serum,nerve growth factor, | en |
dc.relation.page | 138 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2006-07-31 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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