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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳俊宏(Jiun-Hong Chen) | |
dc.contributor.author | Chia-wei Ku | en |
dc.contributor.author | 顧家瑋 | zh_TW |
dc.date.accessioned | 2021-06-13T02:27:34Z | - |
dc.date.available | 2007-02-02 | |
dc.date.copyright | 2007-02-02 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-01-26 | |
dc.identifier.citation | Arnaout M. A., B. Mahalingam, J. P. Xiong (2005) Integrin structure, allostery, and bidirectional signaling. Annu Rev Cell Dev Biol., 21:381-410.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/31057 | - |
dc.description.abstract | 無脊椎動物血球細胞凝集反應為傷口修補及止血之重要機制。本研究以九孔 (Haliotis diversicolor) 的血液細胞為材料,研究血球在凝集反應中的細胞內訊息傳遞機制以及參與分子。實驗結果顯示,類親半乳醣蛋白galectin-like分子可能參與九孔血球細胞的凝集反應,且九孔血液細胞凝集反應需要細胞外鈣離子的存在。而在細胞內訊息傳遞的部分,使用PMA活化整體蛋白激脢C、或使用U73122抑制磷脂脢C或使用Gö6976抑制典型蛋白激脢C都會促進九孔血球細胞凝集反應;然而,使用細胞內鈣離子載體A23187或使用D-sphingosine抑制整體蛋白激脢C會抑制九孔血球細胞凝集反應,顯示和蛋白激脢C相關的訊息途徑參與凝集反應,並且至少有兩類以上,作用相異的蛋白激脢C參與其中。整體而言,細胞內整體磷酸化情況隨細胞凝集過程而增加。而由蝦得到的類TG蛋白質STG1亦對九孔血球細胞凝集反應有引發作用。由本實驗結果推論,galectin在細胞間辨識以及之後的細胞間連結反應上皆扮演重要角色,而至少兩類PKC在九孔血液細胞凝集反應的細胞內訊息傳遞路徑上則扮演著正負兩方雙面調控的角色。 | zh_TW |
dc.description.abstract | Hemocyte aggregation is the main function for wound repair and stop bleeding. In this study, hemocytes of abalone (Haliotis diversicolor) were used to investigate the mechanisms of intracellular signaling transduction on cell aggregation. Based on the results, galectin, one of galactose-sepecific and divalent cation-dependent binding proteins is involved to the aggregation of abalone hemocytes. About the signaling transduction, PMA(phorbol 12-myristate 13-acetate), a broad spectrum PKC(protein kinase C) activator、U73122, a PLC(phospholipase C) inhibitor, Gö6976, classical PKC inhibitor, significantly promoted the aggregation of abalone hemocytes; Contrarily, calcium ionophore A23187 and D-sphingosine, a broad spectrum PKC inhibitor inhibited the aggregation of abalone hemocytes. It is inferred that there are more than one type of PKCs involved in the aggregation of abalone hemocytes. During the period of aggregation of hemocytes, the total phosphorylation level of the hemocyte proteins is increased. In addition, one of STG(shrimp transglutaminase), STG1, secreted from shrimp(Marsupenaeus japonicus) hemocytes could influence the aggregation of abalone hemocytes. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T02:27:34Z (GMT). No. of bitstreams: 1 ntu-96-R93b41006-1.pdf: 493881 bytes, checksum: 69120b3901d97f86bb42d06097fb2bff (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 口試委員會審定書……………………………………………………….....…..………i
誌謝………………………………………………………………………..……….……ii 目錄…………..………………….…………………………….…………………..……iii 圖目錄…………..………………….………………………….……………….…….…v 中文摘要…………………………………………..…………………………..…….…vi 英文摘要………………………………………………..………….….……..………vii 前言………………………………………………………………….1 無脊椎動物之免疫系統及其止血機制……………………………..……………1 實驗動物以及其血球細胞………………………………………………..………3 九孔血液細胞的凝集反應………………………………………….….…………4 九孔血液細胞凝集反應與encapsulation的差異………………………...……4 和血液凝集反應相關的細胞膜上分子………………………………..…………5 和凝集反應相關的細胞內訊息傳遞路徑……………………………....………..6 蛋白激脢C在細胞辨識與連結上扮演的角色…………………………………..7 細胞內蛋白質磷酸化在細胞生理上扮演的角色………………………………..8 材料與方法…………………………………………………………………………….10 化學材料………………………………………………………..………….…….10 實驗動物以及血球細胞取得………………………………………..…………..10 血液細胞凝集反應量化分析……………………………………...……….……11 PKCα活性測定…………………………………………………...………..……11 SDS蛋白質電泳以及西方式墨點染色…………………………………………12 影像處理量化分析以及數據統計處理………………………...………….……13 結果…………………………………………………………………14 九孔血液細胞的凝集反應中細胞形態…………………………………………14 細胞外鈣離子對九孔血液細胞凝集反應的影響………………...……….……14 醣分子對九孔血液細胞凝集反應的影響…………………………………..…..15 九孔血液細胞的凝集反應中蛋白激脢C變化情形…………………….………15 與Galetin-like分子相關細胞內訊息傳導路徑對九孔血液細胞凝集反應的影響…………………………………………………………………………………15 蛋白質激脢C(protein kinase C)對九孔血液細胞凝集反應的影響………..…16 PLC(phospholipase C)相關細胞內訊息傳導路徑對凝集反應的影響…....…16 九孔血液細胞凝集反應中細胞內磷酸化狀況的改變……………….......….…17 討論…………………………………………………………………18 九孔血液細胞凝集反應與止血機制……………………………………………18 細胞膜上醣蛋白在九孔血液細胞凝集反應中扮演的角色……………………19 蛋白激脢C在九孔血液細胞凝集反應中的可能參與機制……………….……20 九孔血液細胞凝集反應中細胞內serine磷酸化狀況改變的可能原因…….…22 其他可能性的九孔血液細胞凝集反應中參與分子…………..……..…………23 九孔血液細胞凝集反應的推論進行過程及分子間關聯性………………....…25 參考文獻…………………………………………………………………….26 圖…………………..………………….………………………………………….……35 附錄 日本對蝦(Marsupenaeus japonicus)類TG蛋白質STG1(shrimp transglutaminase 1)對九孔血球細胞凝集反應有引發作用…………………………………..…..…49 圖 目 錄 圖一、九孔血液細胞的凝集……………………………………….………..………..35 圖二、九孔血液細胞凝集曲線………………………………….……………………36 圖三、細胞外鈣離子影響九孔血液細胞凝集狀況…………………………….……37 圖四、醣相關分子與九孔血液細胞凝集反應……………………………….………38 圖五、EGTA與EDTA對九孔血液細胞凝集反應的影響…………………..…..…39 圖六、醣相關分子PKC活性…………………………….………….…….………...40 圖七、九孔血液細胞凝集過程中PKC活性曲線……………………..……………41 圖八、PMA促進九孔血液細胞凝集反應……………………………….….….……42 圖九、D-sphingosine抑制九孔血液細胞凝集反應……………….………….……43 圖十、U73122抑制九孔血液細胞凝集反應………………………………..………44 圖十一、U73122與A23187促進九孔血液細胞凝集反應中PKC活性…………45 圖十二、A23187與Gö6976對九孔血液細胞凝集反應的影響………….…….…46 圖十三、九孔血液細胞凝集反應中九孔血液細胞內磷酸化現象………………..…47 圖十四、可能的九孔血液細胞凝集反應細胞內訊息路徑示意圖…………......……48 | |
dc.language.iso | zh-TW | |
dc.title | 九孔血液細胞凝集反應中細胞內訊息傳導路徑之研究 | zh_TW |
dc.title | Study of Intracellular Signaling Transduction on Abalone Hemocyte Aggregation | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李心予(Hsinyu Lee),李士傑(Shyh-Jye Lee) | |
dc.subject.keyword | 九孔,無脊椎動物血球細胞,血球細胞凝集反應,蛋白激脢C,磷脂脢C,親半乳醣蛋白galectin, | zh_TW |
dc.subject.keyword | abalone,hemocyte,cell aggregation,PKC,PLC,galectin, | en |
dc.relation.page | 54 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-01-27 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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