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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 何國傑(Kuo-Chieh Ho) | |
dc.contributor.author | Chih-Kuan Chen | en |
dc.contributor.author | 陳志冠 | zh_TW |
dc.date.accessioned | 2021-06-13T02:22:37Z | - |
dc.date.available | 2009-02-02 | |
dc.date.copyright | 2007-02-02 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-01-29 | |
dc.identifier.citation | 林怡璇 (2005) 1997-2002人類及豬隻豬霍亂沙門氏桿菌分離株之分子流行病學及抗藥性趨勢變化研究。中國醫藥學院環境醫學研究所碩士論文
黃斌 (2006) 低溫逆境下綠豆CYP90A2基因的表現及油菜類固醇促進上胚軸伸長的蛋白質體研究。國立台灣大學植物科學研究所博士論文 鄭昆杰 (2006) 靈芝多醣體於人類單核球細胞之分子調控的探討:從基因表現到網路建構。國立台北科技大學生物科技研究所碩士論文 Agtini MD, Ochiai RL, Soeharno R, Lee HJ, Sundoro J, Hadinegoro SR, Han OP, Tana L, Halim FX, Ghani L, Delima, Lestari W, Sintawati FX, Kusumawardani N, Malik R, Santoso TS, Nadjib M, Soeroso S, Wangsasaputra F, Ali M, Ivanoff B, Galindo CM, Pang T, Clemens JD, Suwandono A, Acosta CJ (2006) Introducing Vi polysaccharide typhoid fever vaccine to primary school children in North Jakarta, Indonesia, via an existent school-based vaccination platform. Public Health 120: 1081-1087. Almiron M, Link AJ, Furlong D, Kolter R (1992) A novel DNA-binding protein with regulatory and protective roles in starved Escherichia coli. Genes Dev 6: 2646-2654. Ames GF, Mimura CS, Holbrook SR, Shyamala V (1992) Traffic ATPases: a superfamily of transport proteins operating from Escherichia coli to humans. Adv Enzymol Relat Areas Mol Biol 65: 1-47. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30955 | - |
dc.description.abstract | 霍亂沙門氏桿菌(Salmonella Choleraesuis)是豬隻主要的致病菌之一,偶爾可傳染給人類,並造成系統性的感染。過去多以抗生素來施予治療,但在近年來的研究卻發現,霍亂沙門氏桿菌在台灣等地區出現越來越高的抗藥性比率,尤其對flouroquinolone(FQ)類藥物的抗性正漸漸產生,且遭受感染的人數也逐漸增加中。目前對於霍亂沙門氏桿菌的研究主要以流行病學的基因分子分型為主,較少有基因或蛋白質表現層次的研究。本實驗自疾病管制局從台灣各地所分離的199隻菌種中,挑選了四種不同的分離株,分別是抗FQ的人體分離株,不抗FQ的人體分離株,以及抗FQ的豬隻分離株,不抗FQ的豬隻分離株。試圖以蛋白質體學的方式,對這些分離株進行交叉比對,以找出高低抗藥性與不同寄主間的差異蛋白。具有差異表現的蛋白經過鑑定後,再以QPCR確認這些蛋白在基因表現層次的差異,總共篩選出14個蛋白質,分別是與抗FQ相關的SC064, sat, dps, groS, groL, pduB, malE;與寄主差異相關的sat, dps, groL, pduB, menB, STY3221;在豬與人分離株對FQ抗性有不同反應的基因uspA, pgk, dnaK, rpsA, tuf A,B。這些基因顯示:霍亂沙門氏桿菌對於逆境可能有廣泛性的適應機制,利用dps與熱休克蛋白的調控來適應FQ以及新寄主(人體)。菌體在人體內遭受的逆境可能較在豬隻體內更為強烈,因此有調降生長發育相關的基因來維持生存的情形。菌體在不同寄主體內,可能對抗藥性出現獨立演化的情形,因此人體分離株的抗藥性有普遍較高的現象。 | zh_TW |
dc.description.abstract | Salmonella Choleraesuis is one of the most important pathogens for pigs. It occasionally infects human and causes serious systemic infection. Although antibiotics are the effective drug to prevent and cure the disease, evidences suggest that more and more isolates of S. Choleraesuis in Taiwan have developed drug resistance, especially to flouroquinolone (FQ), and the cases of human infection are increasing. In the last few years, studies have been devoted to genotyping of S. Choleraesuis for epidemiology, but only few have been made at the expression patterns of genes or proteins of S. Choleraesuis. In this study, the proteomics analysis was performed to determine the differential patterns of proteins. Four different isolates were chosen from 199 isolates isolated from Taiwan by Centers for Disease Control: one FQ resistant isolate and one FQ susceptible isolate from human, and one FQ resistant isolate and one FQ susceptible isolate from pig. After the differential displayed proteins were identified by 2D-electrophoresis, the expression level of individual protein was confirmed by Real Time QPCR. Finally, fourteen candidate proteins were identified. Of which, seven proteins could be associated with FQ resistance: SC064, sat, dps, groS, groL, pduB, malE, six proteins could be related to host specificity: sat, dps, groL, pduB, menB, STY3221, and five proteins could be related to FQ resistance in different hosts:sat, dps, groL, pduB, menB, STY3221. The results suggested that S. Choleraesuis could possess universal mechanisms in adaptation to FQ and host by regulating the expression of dps and HSPs. S. Choleraesuis might suffer more stress within human than in pigs, as the genes related to growth and development were down-regulated. S. Choleraesuis might have different drug resistant within different hosts via isolated evolution, therefore human isolates appear to have higher drug resistance. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T02:22:37Z (GMT). No. of bitstreams: 1 ntu-96-R93b42016-1.pdf: 4218126 bytes, checksum: 0d860eb5a1f91eb0a3bcde3be75417b1 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 目錄 I
1 前言 3 1.1 沙門氏桿菌屬的型態與分類 3 1.1.1 沙門氏桿菌屬分類 3 1.1.2 血清型分類 3 1.2 沙門氏桿菌屬的命名 4 1.3 豬霍亂沙門桿氏菌 4 1.4 霍亂沙門氏桿菌的致病性 5 1.5 霍亂沙門氏桿菌的傳遞 6 1.6 霍亂沙門氏桿菌的抗藥性 7 1.6.1 抗藥性 7 1.6.2 台灣的沙門氏桿菌抗藥性 8 1.6.3 抗藥性機轉 9 1.7 蛋白質體技術 11 1.7.1 二維電泳 11 1.7.2 質譜儀分析 12 1.8 研究動機與目的 14 2 材料與方法 15 2.1 實驗流程 15 2.2 材料 15 2.3 方法 16 2.3.1 蛋白質抽取 16 2.3.2 蛋白質定量 16 2.3.3 二維電泳 17 2.3.4 蛋白質螢光染色 19 2.3.5 In-gel digestion 20 2.3.6 蛋白質定序與鑑定 20 2.3.7 RNA抽取 21 2.3.8 cDNA合成 22 2.3.9 Real time QPCR 22 3 結果 25 3.1 分離株的挑選 25 3.2 二維電泳 25 3.2.1 Imagemaster軟體影像分析 25 3.2.2 蛋白定序 25 3.3 RNA層次分析 26 3.3.1 RNA抽取 26 3.3.2 Real-time QPCR 26 3.3.3 基因表現分析 27 3.3.4 結果整理 29 3.4 二維電泳與Real-time PCR趨勢比較 30 4 討論 31 4.1 蛋白質層次 31 4.2 基因表現層次 31 4.3 抗藥性討論 32 4.4 寄主性討論 34 4.5 不同寄主的抗藥差異性 35 4.6 未來展望 37 4.7 可能的實驗誤差 37 5 參考文獻 39 6 圖表 49 7. 附錄 74 | |
dc.language.iso | zh-TW | |
dc.title | 霍亂沙門氏桿菌分離株之比較蛋白質體分析 | zh_TW |
dc.title | Comparative Proteomics Analysis of Salmonella enterica serovar Choleraesuis Isolates | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 阮雪芬(Hsueh-Fen Juan) | |
dc.contributor.oralexamcommittee | 蔡珊珊(San-San Tsay),邱乾順(Chien-Shun Chiou),張照勤(Chao-chin Chang) | |
dc.subject.keyword | 霍亂沙門氏桿菌,抗藥性,寄主性,二維電泳,即時偵測定量聚合脢連鎖反應,獨立演化, | zh_TW |
dc.subject.keyword | Salmonella Choleraesuis,Drug resistance,Host specificity,Real Time QPCR,Isolate evolution, | en |
dc.relation.page | 77 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-01-30 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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