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  1. NTU Theses and Dissertations Repository
  2. 生命科學院
  3. 植物科學研究所
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30787
完整後設資料紀錄
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dc.contributor.advisor林讚標(Tsan-Piao Lin)
dc.contributor.authorFu-Yu Wuen
dc.contributor.author吳馥宇zh_TW
dc.date.accessioned2021-06-13T02:15:42Z-
dc.date.available2007-03-20
dc.date.copyright2007-03-20
dc.date.issued2007
dc.date.submitted2007-02-26
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30787-
dc.description.abstract摘要
本研究藉由粒線體DNA(mtDNA)多型性的變異,來探討廣泛分佈於台灣海拔兩千八百公尺以上的台灣冷杉(Abies Kawakamii)之親緣地理關係。本研究選取大霸尖山、雪山主東峰、北二段無名山、合歡東峰、奇萊南華、玉山、關山等八個樣區,選取67個單株進行研究,研究中共測試十八對Primer,只有兩對Primer FO303/FO304及nad5/1-2a可以呈現單一Band,序列比對後發現這兩段基因序列毫無變化。除了冷杉推測可能與台灣冷杉正處於冰河時期過後往高海拔退縮,族群正面臨瓶頸現象有關。
zh_TW
dc.description.abstractThe spatial pattern of mitochondrial DNA(mtDNA)variation in Abies kawakamii (Hayata) Ito(Pinaceae)was studied in 67 trees collected in Taiwan Alpine(8 population).By sequencing 2 mtDNA intron fragmens using 2 primers(FO303/FO304 and nad5/1-2a).We revealed a total of 2675bp with no polymorphic site(FO303/FO304:746bp , nad5/1-2a:1929bp).Among them, there is no mutation and deletion.This outcome probably result from demograohic history of rapid contraction that eliminated the diversity.en
dc.description.provenanceMade available in DSpace on 2021-06-13T02:15:42Z (GMT). No. of bitstreams: 1
ntu-96-R91226014-1.pdf: 405167 bytes, checksum: 40c731577b54f5ec5d1aeba6c3f9efab (MD5)
Previous issue date: 2007
en
dc.description.tableofcontents致謝…………………………………………………………………………………I
中文摘要……………………………………………………………………………II
英文摘要……………………………………………………………………………III
目錄…………………………………………………………………………………IV
壹、 前言………………………………………………………………………………1
貳、 材料與方法…………………………………………………………………… 11
一、樣本的採集…………………………………………………………………11
二、實驗方法……………………………………………………………………11
(一)總DNA的萃取……………………………………………………11
(二)DNA定量………………………………………………………….12
(三)聚合酵素連鎖反應(PCR)與定序………………………………12
三、試劑配方……………………………………………………………………15
四、資料分析……………………………………………………………………17
(一)序列分析……………………………………………………………17
(二)遺傳變異分析………………………………………………………17
(1)單套型歧異度(h)及核甘酸歧異度(π)………………17
(2)中性假說測試………………………………………………18
(三)族群次結構分析……………………………………………………19
(四)空間結構分析 ……………………………………………………22
參、結果
一、序列分析……………………………………………………………………24
肆、討論………………………………………………………………………………25
伍、參考文獻…………………………………………………………………………28
附表………………………………………………………………………………… 36
附圖………………………………………………………………………………… 37
dc.language.isozh-TW
dc.title台灣冷杉粒線體DNA多型性之研究zh_TW
dc.titleThe mitochondrial DNA Polymorphism of Abies Kawakamii in Taiwanen
dc.typeThesis
dc.date.schoolyear95-1
dc.description.degree碩士
dc.contributor.oralexamcommittee黃士穎(Shih-Ying Hwang),何國傑(Kuo-Chieh Ho)
dc.subject.keyword台灣冷杉,粒線體,親緣地理,zh_TW
dc.subject.keywordAbies,Fir,mtDNA,en
dc.relation.page38
dc.rights.note有償授權
dc.date.accepted2007-02-27
dc.contributor.author-college生命科學院zh_TW
dc.contributor.author-dept植物科學研究所zh_TW
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