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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 丘臺生(Tai-Sheng Chiu) | |
dc.contributor.author | Chung-Yu Tang | en |
dc.contributor.author | 湯宗瑜 | zh_TW |
dc.date.accessioned | 2021-06-13T02:13:14Z | - |
dc.date.available | 2008-07-16 | |
dc.date.copyright | 2007-07-16 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-06-05 | |
dc.identifier.citation | Anonymous. 2006. Fisheries Statistical Yearbook Taiwan Area 2005. Taipei: Fisheries Agency, Council of Agriculture, Executive Yuan.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30714 | - |
dc.description.abstract | 鯖屬之花腹鯖(Scomber australasicus)與白腹鯖 (S. japonicus)常出現在台灣附近海域。 兩者皆為大洋性魚類,並且成群洄游,進行季節性的遷徙,以達到攝餌或產卵之目的。 這兩種鯖魚為台灣近海漁業中重要的漁業資源,成為鯖鰺圍網及定置網漁業中之主要漁獲物,尤其以花腹鯖數量較多。 對此二物種之漁業施以管理,並對此資源加以保育,以維持資源利用之永續性為目前重要課題,因此物種的判別以及系群的結構皆為值得探究的問題。 在台灣,白腹鯖過去的研究甚少,而花腹鯖在系群研究上數量較多,但結論不一。
本論文利用形態測量分析(morphometric analysis)及分子特徵分析 (molecular markers analysis)進行物種之判別與系群結構的研究。 物種判別使用粒腺體DNA中之細胞色素b的全長序列,並由RFLP-PCR提供一快速的鑑定方法,而系群結構的問題則利用微隨體基因座進行分析。 由統計分析形態測量資料無法清楚的區分花腹鯖和白腹鯖,但利用RFLP-PCR分析色素細胞b序列的結果,可以明確地將此二近似物種加以分辨。 系群結構的部分,花腹鯖的形態測量分析結果無法達到清楚的辨別能力,但由微隨體基因座的分析結果可得到顯著的差異顯示在台灣附近應為兩個系群,分別是包括東海的北邊群以及東海之外南邊群。 無論形態資料分析抑或微隨體基因座的結果都指出白腹鯖只有單一系群存在台灣附近海域。 | zh_TW |
dc.description.abstract | In Taiwanese neritic waters, blue mackerel (Scomber australasicus) and chub mackerel (S. japanicus) are found pelagic and migrating in shoals for feeding and spawning. These two species are important fishery resources for purse seiners and set-netters. For the purpose of fishery management and resource conservation, it is essential to discriminate two species and study their intra-population structures. There are a few studies related to the population structure of S. australasicus, and much fewer for S. japonicus. As a whole, the stock status of blue and chub mackerels in the hydrographic region of Taiwan is still far from comprehensive for management purpose.
In this study, my objectives are 1) to clarify inter-specific relationship of S. australasicus and S. japonicus, and 2) to explore intra-species structures in different hydrographic regions. Statistical analysis on morphometric measurements and RFLP-PCR analysis on the complete sequence of cytochrome b gene (cyt-b) were used to provide a quick, convenient and precise method to identify S. australasicus and S. japonicus. Morphometric measurements and microsatellite polymorphisms were used to study population differences among hydrographic regions. The findings indicated the lack of clear-cut resolution in morphometric analysis for species discrimination and stock structure identification. Nonetheless, RFLP-PCR analysis based on cyt-b on can be an effective agent for species identification. Analysis of population structure based on microsatellite polymorphism revealed that two stocks of S. australasicus off Taiwan should be considered – one located in the northern water and the other in the southern. On the other hand, one unit stock of S. japonicus is suggested for management purpose. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T02:13:14Z (GMT). No. of bitstreams: 1 ntu-96-R94b41002-1.pdf: 772864 bytes, checksum: ce1512db5b4d9eb30dabad7b010d5f15 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | Contents
Contents …………………………………………………………………. Ⅰ 摘要 ……………………………………...…………………………….... Ⅲ Abstract …………………………………………………………….….… Ⅳ 1. Introduction 1.1 Identification of blue mackerel and chub mackerel ………………… 1 1.2 Geographical distribution …………………………………………… 2 1.3 Ecological and biological features ………………………………….. 2 1.4 Mackerel fisheries in Taiwan …..………………………………….. 3 1.5 Stock structure of mackerels in Taiwanese waters ………………….. 3 1.6 Hydrographic conditions in the vicinity of Taiwan island ………….. 5 1.7 Morphometric analysis ……………………………………………… 5 1.8 Molecular markers ………………………………………………….. 6 1.8.1 Cytochrome b gene …………………………………..………….. 6 1.8.2 Microsatellite …………………………………..………………… 7 1.9 Objectives of study .……………….………………………………… 8 2. Materials and Methods 2.1 Sample collection ……………………………………………….…… 9 2.2 Morphometric analysis ………………………………………….…… 9 2.3 Genetic analysis …………………………………………..…………. 9 2.3.1 DNA extraction ……………………………………..……………. 9 2.3.2 Cytochrome b gene amplification and PCR-RFLP ………………. 10 2.3.3 Microsatellite isolation ……………………………………..…….. 11 2.3.4 Genotyping ………………………………………………..……… 12 2.4 Data analysis ……………………………………………………….... 12 2.4.1 Multivariate analyses ……………………………………..……… 12 2.4.2 Cytochrome b sequences and PCR-RFLP ……………………….. 13 2.4.3 Microsatellite data ………………………….......................……… 14 3. Results 3.1 Scomber species discrimination ……………………………………... 15 3.1.1 Morphometric analysis ………………………………………….... 15 3.1.2 Molecular analysis ……………………………………………….. 15 3.2 Stock structure of Scomber australasicus …………………………… 16 3.2.1 Morphometric analysis ………………………………………….... 16 3.2.2 Microsatellite marker …………………………….………………. 17 3.3 Stock structure of Scomber japonicus ………………………………. 18 3.3.1 Morphometric analysis ………………………………………….... 18 3.3.2 Microsatellite marker …………………………….………………. 18 4. Discussion 4.1 Species identification …………………………………..…..………… 20 4.2 Population structure ………………………………….……………… 21 4.2.1 Scomber australasicus ……………………………….. …..…..… 21 4.2.2 Scomber japonicus ……..…………………………………………. 23 5. References …………………………………………………………... 25 Tables …………………………………………………………………… 32 Figure Legend …………………………………………………………. 50 Figures …………………………………………………………………... 52 | |
dc.language.iso | en | |
dc.title | 利用型態及遺傳變異分析探討台灣週邊水域鯖魚之種間判別與系群關係 | zh_TW |
dc.title | Discrimination of Scomber spp. and their Stock Structures in the Hydrographic Regions around Taiwan by Morphometric and Molecular Analyses | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 詹榮桂(Rong-Quen Jan),于宏燦(Hon-Tsen Yu),廖正信(Cheng-Hsin Liao),陳志忻(Chih-Shin Chen) | |
dc.subject.keyword | 鯖屬,系群,微隨體,型態分析, | zh_TW |
dc.subject.keyword | Scomber,stock,morphometric,microsatellite, | en |
dc.relation.page | 64 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-06-05 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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