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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 董成淵(Chen-Yuan Dong) | |
dc.contributor.author | Chi-Hsiu Huang | en |
dc.contributor.author | 黃啟修 | zh_TW |
dc.date.accessioned | 2021-06-13T02:04:36Z | - |
dc.date.available | 2007-07-20 | |
dc.date.copyright | 2007-07-20 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-03 | |
dc.identifier.citation | 1. 李宣書.淺談組織工程.物理雙月刊 24,430(2002.6)
2. 巧奪天工的人類智慧──組織工程�科學發展 2002年8月,356期 3. 幫細胞蓋一個家──組織工程用支架�科學發展 2002年8月,356期 4. 關節軟骨再生-化不可能為實現/科學發展 2002年8月,356期 5. 組織工程.林峰輝 著 6. http://www.chemtech.com.tw/Creative.php?mode=detail&id=22 7. 組織學.藝軒出版社 8. Cartilage tissue engineering :its potential and uses Curr Opin Rheumatol 18 :64-73 9. Advancing cartilage tissue engineering :the application of stem cell technology Current Opinion in Biotechnology 2005,16:503-509 10. History of tissue engineering and a glimpse into its future.Tissue Engineering Volume 12, Number5,2006 11. Imaging human bone marrow stem cell morphogenesis in polyglycolic acid scaffold by multiphoton microscopy.Tissue Engineering Volume 12,Number 10,2006 12. Biomaterials in cartilage and bone tissue engineering.Current Opinion in Orthopaedics 2004,15:347-354 13. Nonlinear Optics 2rd edition,Robert W.Boyd 14. Masksymilian Pluta,”Advanced Light Microscopy:1.Principles and Basic Properties”Elsevier(1988) 15. Masksymilian Pluta,”Advanced Light Microscopy:2.Specilized Methods”, Elsevier(1989) 16. Dong, CY, Koenig, K, So, P, Characterizing point spread functions of two-photon fluorescence microscopy in turbid medium, J BIOMED OPT 8 (3): 450-459 JUL 2003 17. So PTC, Dong CY, Masters BR, et al ,Two-photon excitation fluorescence microscopy ANNUAL REVIEW OF BIOMEDICAL ENGINEERING 2: 399-429 2000 18. J.D Jackson “Classical Electrodynamics 3rd edition “ Wiley(1998) 19. 王俊欽“運用非線性光學顯微術於診斷人類膀胱癌與活體裸鼠早期癌症影像技術之發展-國立台灣大學物理學研究所碩士論文 “(2005) 20. Max Born and Emil Wolf“Principles of Optics 7rd edition“Cambridge 21. 雙光子顯微術於生物組織的應用.李宜真(台大物理學系)、蘇子正(麻省理工機械) 22. 雷射原理與應用.林三寶 編著 23. 林子于“應用非線性光學顯微術於生物可降解材料及癌症診斷“-國立台灣大學物理學研究所碩士論文 24. Multi-Material Scaffolds For Tissue Engineering.Macromol.Symp. 2005,227,345-355 25. Scaffolds in tissue engineering bone and cartilage.Biomaterials 21 (2000) 2529-2543 26. Biodegradable Polymer Scaffolds Cartilage Tissue Engineering.Clinical Orthopaedics and Related Research Number 391S,pp,S251-S270 27. Chitosan scaffolds:Interconnective pore size and cartilage engineering. Acta Biomaterialia 2 (2006) 313-320 28. Recent advances and current developments in tissue scaffolding. Bio-Medical Materials and Engineering 15 (2005) 159-177 29. Performance of collagen sponge as a 3-D scaffold for tooth-tissue engineering.Biomaterials 27 (2006) 3238-3248 30. A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells.Biomaterials 26 (2005) 599-609 31. Tissue Engineering of Cartilage in Space.Proceedings of the National Academy of Sciences of the United States of America,Vol.94 No.25,pp. 13885-13890 32. Experimental Model for Cartilage Tissue Engineering to Regenerate the Zonal Organization of Articular Cartilage.OsteoArthritis and Cartilage (2003) 11,653-664 33. ChemTech 化工產業知識網-生技專欄 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30468 | - |
dc.description.abstract | 雙光子顯微術由於使用波長較長的近紅外光作為激發光源,所以具有穿透深度深、較少的光破壞,且可以激發所有可見光波段的自發螢光和二倍頻訊號,使得我們可以直接透過自發螢光和二倍頻訊號來觀測樣品,而不需要將樣品切片和染色。因此,我希望能將這種非線性光學顯微術技術來應用於組織工程上的觀測與分析。
在組織工程裡,培養體外軟骨組織用來移植一直是組織工程極欲努力研究的目標。最常用的方式就是將間葉幹細胞種入三維可分解和可降解的生物相容性鷹架材料,然後再加入適當的訊息因子-TGF-β3來誘導細胞產生膠原蛋白,進而長成軟骨組織。在這個研究裡,我們將間葉幹細胞種入幾丁聚醣鷹架中,加入訊息因子TGF-β3來誘發軟骨組織的產生,並且利用雙光子螢光和二倍頻顯微術來取得這個過程中的影像。 過程中會發現,藉由加入TGF-β3刺激所產生的膠原蛋白,在四個禮拜左右的期間就可以被觀察到。膠原蛋白的產生與時間並非成線性的關係,大約到了第二十一天的時候就達到飽和了,且膠原蛋白產生會依據鷹架孔洞的形狀而生長。 | zh_TW |
dc.description.abstract | Multiphoton microscopy ,using longer wavelength of near infrared light as excitation light source has deeper penetration and less photodamage. Besides,it can capable all visible autofluorescence and second harmonic generation,making us observe specimens through autofluorescence and second harmonic generation directly without slicing and dying. Hence,I hope to apply this technology of nonlinear optical microscopy to observe and analysize in tissue engineering.
One important goal in tissue engineering is cultiviating cartilage tissue for transplantation.The most common method to cultivate cartilage is to seed human bone marrow mesenchymal stem cells on three dimensional biocompatiable and biodegradable scaffold materials,then add the signal factor TGF-β3 to induce the cells to produce collagen that would grow into cartilage tissue.In this study,we seeded mesenchymal stem cells on a chitosan scaffold and added TGF-β3 to induce cartilage tissue producing and image the process using two-photon fluorescence and second harmonic generation microscopy. In the course of imaging,we found that the production of collagen by the addition of TGF-β3 can be followed over a four-week period. The growth of collagen is not linear with time,and it approaches saturation at around 21th day.And the collagen fibers have a tendency to grow following the shape of scaffold pores. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T02:04:36Z (GMT). No. of bitstreams: 1 ntu-96-R94222052-1.pdf: 21849695 bytes, checksum: a8b5fd8d6fffe03e350357bd7fa33ce6 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 致謝
目錄 1 圖目錄 2 英文摘要 4 中文摘要 5 第一章 緒論 1.1 研究動機 6 1.2組織工程簡介 6 1.3 軟骨組織工程 13 第二章 實驗原理 2.1 雙光子螢光原理 16 2.2 二倍頻原理 21 2.3 其他基本原理 23 第三章 實驗儀器裝置 3.1 多光子顯微鏡系統 26 3.2 激發光源 28 3.3 光學元件 28 3.4 顯微鏡系統與訊號接收 29 第四章 實驗內容和討論 4.1 樣品介紹 30 4.2樣品固定 32 4.3實驗方法 34 4.4實驗結果與討論 35 第五章 總結與未來展望 52 第六章 參考資料 53 | |
dc.language.iso | zh-TW | |
dc.title | 運用雙光子和二倍頻顯微術來研究人類骨髓間葉幹細胞在幾丁聚醣鷹架上的組織形成 | zh_TW |
dc.title | Utilizing two-photon fluorescence and second harmonic generation microscopy to study human bone marrow mesenchymal stem cell morphogenesis in Chitosan scaffold | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李宣書(Hsuan-Shu Lee),曹培熙,林頌然 | |
dc.subject.keyword | 雙光子,二倍頻,組織工程,間葉幹細胞,幾丁聚醣, | zh_TW |
dc.subject.keyword | Two photon,Second harmonic generation,Tissue Engineering,Mesenchymal stem cell,Chitosan, | en |
dc.relation.page | 55 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-04 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 物理研究所 | zh_TW |
顯示於系所單位: | 物理學系 |
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