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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 朱善德 | |
| dc.contributor.author | Wen-Wei Li | en |
| dc.contributor.author | 李文瑋 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:44:23Z | - |
| dc.date.available | 2007-07-18 | |
| dc.date.copyright | 2007-07-18 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-10 | |
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Orrenius S, Z.B., Nicotera P., REGULATION OF CELL DEATH: THE CALCIUM–APOPTOSIS LINK Nat Rev Mol Cell Biol., 2003. 4(7): p. 552-65. 49. Nakayama, T., et al., The regulatory domain of the inositol 1,4,5-trisphosphate receptor is necessary to keep the channel domain closed: possible physiological significance of specific cleavage by caspase 3. Biochem. J., 2004. 377(2): p. 299-307. 50. Bubici, C., et al., Mutual cross-talk between reactive oxygen species and nuclear factor-kappa B: molecular basis and biological significance. Oncogene, 2006. 25(51): p. 6731-6748. 51. Zhuang, S. and R.G. Schnellmann, A Death-Promoting Role for Extracellular Signal-Regulated Kinase. J Pharmacol Exp Ther, 2006. 319(3): p. 991-997. 52. Wada T, P.J., Mitogen-activated protein kinases in apoptosis regulation. Oncogene. , 2004. 23(16): p. 2838-49. . 53. 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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/30219 | - |
| dc.description.abstract | 24p3蛋白屬於疏水性分子結合蛋白，為小白鼠動情週期中子宮外泌蛋白之一。實驗室近期研究指出小鼠子宮24p3蛋白可以透過cytochrome c的釋放以及活化caspase 3造成人類子宮內膜細胞的凋亡，顯示24p3蛋白於雌性生殖系統中可能扮演重要角色。為了解24p3於生殖系統中誘發細胞凋亡的機制，利用純化自小鼠子宮的24p3蛋白以及人類子宮內膜細胞為模式研究誘發細胞凋亡過程中參與反應之因子，發現24p3蛋白於無血清狀態下可透過NADPH oxidase造成胞內氧化壓力增加並可能藉此調節MAPK蛋白磷酸化狀態及造成胞內鈣離子濃度的改變，暗示MAPK蛋白的磷酸化狀態與鈣離子濃度的增加可能與細胞的死亡有關，此過程中p53、bcl-2、bax等基因表現並未受到調節。推測24p3蛋白透過不同於Bcl-2/Bax之凋亡路徑促成細胞凋亡。 | zh_TW |
| dc.description.abstract | 24p3 protein, a lipocalin, is one of the secretory proteins during mouse estrous cycle. According to the data of our laboratory, reveals that 24p3 protein induces apoptosis of human endometrial carcinoma cell line via releasing cytochrome c from mitochondria and activating caspases. These results suggest that 24p3 protein plays an unique role in female reproduction. To elucidate the mechanism of 24p3 protein induced apoptosis in reproduction system, mice uterine 24p3 protein and human endometrial cell line (RL95-2) were used in this study. After treatment of RL95-2 cells, 24p3 protein creates an oxidative intracellular environment that may trigger the changes of MAPK activity and elevation of intracellular calcium via NAPDH oxidase. These data suggest that MAPK and calcium may be correlated to cell death. Without significant changes in mRNA levels of p53, bcl-2, and bax could be observed in 24p3 protein-treated cells. These findings revealed that the eliciting of cell physiological response by 24p3 protein in a novel pathway. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:44:23Z (GMT). No. of bitstreams: 1 ntu-96-R94b46020-1.pdf: 1497168 bytes, checksum: da7c85b2b5b1930cd6aac14309076277 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目錄
摘要 0 Abstract ii 縮寫表 iii 目錄 vi 圖目錄 viii 前言 1 藥品與器材 6 材料 6 藥品 6 儀器 8 試劑與常用溶液 9 方法 11 24p3純化 11 誘發小鼠子宮液分泌以及收集樣本 11 分子篩膠體層析法 11 逆相高效液相層析法 (Reverse phase HPLC) 12 細胞株培養 12 繼代培養 12 細胞冷凍與解凍 12 蛋白質萃取 13 蛋白質定量 13 Sodium dodecyl sulfate polyacrylamide gel electrophoresis, SDS-PAGE 13 西方墨點法 13 胞內ROS (reactive oxygen species)測定 14 GSH (glutathione)測定 14 Caspase 3活性測定 15 RNA萃取 15 DNase digest 15 即時定量反轉錄聚合酶連鎖反應(Realtime RT-PCR) 16 胞內鈣離子濃度測定 16 以螢光分光光度計偵測鈣離子濃度 17 以流式細胞儀偵測鈣離子濃度 17 結果 18 24p3蛋白與RL95-2細胞胞內reactive oxygen species (ROS)的關係 18 24p3蛋白對p53、Bcl-2以及Bax基因表現量的影響 19 24p3蛋白影響細胞內鈣離子濃度變化 19 24p3蛋白細胞內鈣離子濃度變化與內質網(endoplasmic redictium, ER)上inositol 1,4,5-triphosphate (IP3)受器、Ryanodine受器(RyRs)的關係 20 24p3蛋白對Mitogen-activated protein kinase (MAPK)的影響 21 ERK蛋白於24p3蛋白細胞凋亡路徑中扮演的角色 21 討論 22 實驗結果 27 參考文獻 45 附錄 50 附錄一. Tris/Glycine SDS-Polyacrylamide Gel 電泳膠體配方。 50 附錄二. Realtime RT PCR primer序列 51 | |
| dc.language.iso | zh-TW | |
| dc.subject | 鈣 | zh_TW |
| dc.subject | 疏水性分子結合蛋白 | zh_TW |
| dc.subject | 凋亡 | zh_TW |
| dc.subject | 活性氧 | zh_TW |
| dc.subject | 24p3 | en |
| dc.subject | MAPK | en |
| dc.subject | calcium | en |
| dc.subject | ROS | en |
| dc.subject | apoptosis | en |
| dc.subject | lipocalin | en |
| dc.title | 子宮24p3蛋白誘發子宮內膜細胞凋亡之研究 | zh_TW |
| dc.title | Study of uterine 24p3 protein induced apoptosis in endometrial carcinoma cell line | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 曾婉芳,余榮熾,張?仁 | |
| dc.subject.keyword | 疏水性分子結合蛋白,凋亡,活性氧,鈣, | zh_TW |
| dc.subject.keyword | lipocalin,24p3,apoptosis,ROS,calcium,MAPK, | en |
| dc.relation.page | 51 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-11 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科學研究所 | zh_TW |
| 顯示於系所單位： | 生化科學研究所 | |
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