矽晶圓表面改質對紅血球與細菌的固定影響探討

Abstract

本實驗使用矽晶圓的表面改質分成三大部份，第一個部份是經過Poly-L-lysine和戊二醛（Glutaraldehyde）表面處理，用來固定紅血球，透過原子力顯微鏡來觀測其外貌。第二個部份是將矽晶圓表面直接物理吸附膠原蛋白，將大腸桿菌（Ｅ.coli）放在的膠原蛋白上附著固定，在恆溫生物樣品盒下，透過光學顯微鏡可以觀察其固定和分裂的現象。 第三部份:利用軟微影的Microcontact printing技術將 MHA的硫醇與金（SH-Au）鍵結形成自我排列單分子層（SAM），MOU鈍化後使用Poly-L-lysine鍵結於MHA (NH3+-COO-)，將桿菌固定在表面改質的矽晶圓，透過光學顯微鏡和原子力顯微鏡觀測大腸桿菌的形狀和排列秩序。 結果顯示原子力顯微鏡可以在非真空的大自然環境中去觀測紅血球在矽晶圓表面上隨著時間的形狀變化，藉此方法可以推估死亡時間。 而透過矽晶圓表面的改質處理後，用來固定生物細胞可應用在生物晶片的發展，未來進一步發展控溫控溼生物樣品盒在各種TEM或者SEM等的顯微鏡下，可以用來觀測活體生物細胞分裂，並進行其他生物方面相關研究。

The experiment is about the surface modified silicon wafer which was conducted in three parts: first part is to fix the red blood cells on Poly-L-lysine and Glutaraldehyde modified silicon surface, second part is using optical microscope to observe the fission of E.coli fixed on the collagen modified silicon surface in the temperature controlled chamber. Third part is using Microcontact printing technology to make 16-Mercaptohexadecanoic acid (MHA) and Au linking（SH-Au）together to form self-assembled monolayer（SAM）. Take 11-Mercapto-1-undecanol (MOU) to passivate the other surface. Then put Poly-L-lysine on MHA (NH３+-COO-). Eventually E.coli will immobilize on the modified silicon surface. So we can observe the shape and the arrangement order by optical microscope and Atomic Force Microscope（AFM）. The results show that we can use AFM to image the shape of the red blood cells when they are changing with time on the silicon surface in the natural environment. And this experiment affords a new method to estimate the time of death. The surface modified silicon wafer to immobilize the biological cells can be used in the biochips. To make progress in the environment control chamber in the TEM or SEM in the future can observe the fission of biological cells and other biological research.