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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 蘇慧敏 | |
| dc.contributor.author | Hsiang-Ju Huang | en |
| dc.contributor.author | 黃湘茹 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:22:17Z | - |
| dc.date.available | 2012-08-08 | |
| dc.date.copyright | 2007-08-08 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-18 | |
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J Cell Physiol. 125:61-71. Wilson, J.W., A.E. Wakeling, I.D. Morris, J.A. Hickman, and C. Dive. 1995. MCF-7 human mammary adenocarcinoma cell death in vitro in response to hormone-withdrawal and DNA damage. Int J Cancer. 61:502-8. Wynder, E.L., D.P. Rose, and L.A. Cohen. 1986. Diet and breast cancer in causation and therapy. Cancer. 58:1804-13. Zoratti, M., and I. Szabo. 1995. The mitochondrial permeability transition. Biochim Biophys Acta. 1241:139-76. 中華民國公共衛生癌症登記小組網站 行政院衛生署民國九十五年死因統計資料 駱中郁, 民國94年, 探討紅花籽油攝取在促進荷爾蒙依賴型乳癌之作用機制及乳癌生成之關鍵時期生理學研究所,臺灣大學. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29864 | - |
| dc.description.abstract | 目前針對花生四烯酸（arachidonic acid，AA C20:4 ω6）的研究指出，AA在細胞中具有兩種截然不同的功能。一為增加氧化壓而導致細胞凋亡，另一方面則具有促使細胞增生、乃至癌化之現象。然而現今對AA在乳癌細胞上的作用並不清楚。因此本實驗之目的為：以人類乳腺癌細胞，MCF-7，為材料，探討AA是否會引發乳癌細胞進行凋亡。實驗方法為：利用2% FBS DMEM中補充不同濃度之AA處理六小時，檢測其影響。
結果發現：給予MCF-7細胞0、10、25、50、100、200 μM 的AA處理六小時後，可改變MCF-7細胞中各脂肪酸之比例。其中AA佔總脂肪酸的比例可由控制組（AA 0 μM）之7.4%上升至給予50 μM時的15％，但超過50 μM之後，AA在細胞中之比例則不再增加。若分別給予0、10、50、100 μM的AA處理六小時後，相較於控制組，AA的處理並不會改變MCF-7細胞的存活率。以流式細胞儀偵測給予0、10、50、100 μM的AA處理四、六、八、十六小時後的粒線體膜電位，發現MCF-7細胞之粒線體膜電位並未出現去極化現象。同時給予MCF-7細胞處理0、10、50、100 μM 的AA六小時後，與細胞凋亡相關之酵素：caspase-7與caspase-9均無顯著增加。 本實驗結果證明，在人類乳腺癌細胞上，給予AA處理六小時內並不會造成其存活率之下降，也不會影響細胞凋亡之兩項指標：粒線體膜電位去極化、以及procaspase-7、procaspase-9的活化。顯示AA對於人類乳腺癌細胞並無引發細胞凋亡的效果。 | zh_TW |
| dc.description.abstract | Arachidonic acid (20:4ω6, AA) may involve the cell proliferation or apoptosis shown in many studies. However, it is still unknown whether AA could induce apoptosis in MCF-7, a human breast cancer cell lines. The aim of this study was to investigate effect of AA on apoptosis in MCF-7. Cells was cultured in DMEM with 2% FBS for 42 hours, followed by AA supplementation for 6 hours.
It was found that the AA level in MCF-7 was increased from 7.4% of total fatty acid up to 15% with the increasing AA supplementation to 50 μM. However, the viability of MCF-7 was not changed compared with controls with 10, 50, and 100 μM AA supplementation. The mitochondrial membrane potential was not altered in MCF-7 supplemented with 10, 50, and 100 μM AA for 4, 6, 8 or 16 hours. The activation of procaspase-7 and procaspase-9 were not increased with the increasing AA treatment. In conclusion, the increased AA level in MCF-7 did not change its viability, mitochondrial membrane potential, caspase-7 and caspase-9. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:22:17Z (GMT). No. of bitstreams: 1 ntu-96-R94441005-1.pdf: 1008072 bytes, checksum: 451a2649b28ce302aed5f92ea3748e1b (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 中文摘要 I
Abstract II 第壹章、緒論 1 一、研究動機 1 二、實驗假設 2 三、實驗設計 2 四、重要性 2 第貳章、文獻回顧 3 一、女性乳癌簡介 3 二、多元不飽和脂肪酸 4 三、花生四烯酸與其代謝產物 5 四、花生四烯酸與細胞增生、死亡之相關性 7 五、花生四烯酸與細胞凋亡之相關性 8 第參章、材料與方法 11 一、實驗材料 11 二、花生四烯酸儲備品之配製 11 三、脂肪酸萃取與甲基化反應 12 四、脂肪酸成分分析 13 （一）、氣相層析儀（Gas chromatography）簡介 13 （二）、操作流程 13 （三）、數據處理 14 五、細胞存活率檢測 15 六、粒線體膜電位偵測 15 （一）、流式細胞儀（Flow cytometry）簡介 15 （二）、螢光染劑DIOC6(3)與PI之製備 16 （三）、DIOC6(3)與PI雙染之原理 16 （四）、實驗流程 17 （五）、流式細胞儀之應用軟體與操作流程 18 （六）、數據處理 21 七、西方墨點法 21 （一）、實驗條件 21 （二）、蛋白質萃取 22 （三）、製作蛋白質標準曲線、測量樣本蛋白質濃度 23 （四）、SDS膠體電泳（SDS-PAGE）分析 23 （五）、SDS-PAGE流程 25 （六）、蛋白質轉印流程 26 （七）、抗體反應 27 （八）、暗房壓片與定量 29 八、統計方法 30 九、實驗器材、藥品一覽表 30 第肆章、實驗結果 33 一、脂肪酸成分分析 33 二、細胞存活率檢測 36 三、粒線體膜電位偵測 37 四、西方墨點法分析 41 第伍章、討論 43 一、實驗設計部分 43 （一）、實驗材料的選擇 43 （二）、AA的配製與儲存方法 43 （三）、培養條件 44 （四）、處理時間 45 二、實驗結果部分 45 （一）、AA在癌細胞與正常細胞間之比例差異 45 （二）、AA對細胞存活率的影響 47 （三）、AA對粒線體膜電位的影響 48 （四）、AA對caspase-7與caspase-9的影響 50 第陸章、結論 52 第柒章、文獻回顧 53 | |
| dc.language.iso | zh-TW | |
| dc.subject | 花生四烯酸 | zh_TW |
| dc.subject | 細胞凋亡 | zh_TW |
| dc.subject | 粒線體膜電位 | zh_TW |
| dc.subject | caspase-7 | zh_TW |
| dc.subject | caspase-9 | zh_TW |
| dc.subject | apoptosis | en |
| dc.subject | caspase-9 | en |
| dc.subject | caspase-7 | en |
| dc.subject | mitochondrial membrane potential | en |
| dc.subject | Arachidonic acid (20:4ω6) | en |
| dc.title | 探討花生四烯酸對人類乳腺癌細胞進行細胞凋亡之研究 | zh_TW |
| dc.title | The effect of arachidonic acid on apoptosis studied in human breast cancer cell line. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃青真,呂紹俊,胡孟君 | |
| dc.subject.keyword | 花生四烯酸,細胞凋亡,粒線體膜電位,caspase-7,caspase-9, | zh_TW |
| dc.subject.keyword | Arachidonic acid (20:4ω6),apoptosis,mitochondrial membrane potential,caspase-7,caspase-9, | en |
| dc.relation.page | 57 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-18 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 生理學研究所 | zh_TW |
| Appears in Collections: | 生理學科所 | |
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