請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29801
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 吳應寧副教授(Ying-Ling Wu) | |
dc.contributor.author | Wen-Chi Chang | en |
dc.contributor.author | 張文綺 | zh_TW |
dc.date.accessioned | 2021-06-13T01:19:26Z | - |
dc.date.available | 2009-07-02 | |
dc.date.copyright | 2007-07-24 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-17 | |
dc.identifier.citation | 1. Akita K, Oduno M, Enya M, Imai S, Moriwaki H, Kaeada N and Suzuki Y. Impaired liver regeneration in mice by lipopolysaccharide via TNF-α/Kallikrein-mediated activation of latent TGF-β. Gastroenterology 2002;123:352-64.
2. Akira S, Takeda K and Kaisho T. Toll-like receptors: critical proteins linking innate and acquired immunity. Nat Immunol 2001;2:675-80. 3. Arbuthnot P and Kew M. Hepatitis B virus and hepatocellular carcinoma. Int J Exp Pathol 2001;82:77-100. 4. Been J and Schneider RJ. Hepatitis B virus HBx protein activates RAS-GTP complex formation and establishes a RAS, RAF, MAP kinase signaling cascade. Proc Natl Acad Sci 1994; 91:10350-4. 5. Beg AA. Review: Endogenous ligands of Toll-like receptors: implications for regulating inflammatory and immune responses. Trends Immunol 2002;23:509-12. 6. Bilzer M, Roggel F and Gerbes AL. Review: Role of Kupffer cells in host defense and liver disease. Liver International 2006;26:1175–86. 7. Black D, Bird MA, Hayden M, Schrum LW, Lange P, Samson C, Hatano E, Rippe RA, Brenner DA and Behrns KE. TNF-alpha-induced hepatocyte apoptosis is associated with alterations of the cell cycle and decreased stem loop binding protein. Surgery 2004;135:619-28. 8. Blaszczyk T, Krzyzanowska J and Lamer-Zarawska E. Screening for antimycotic properties of 56 traditional Chinese drugs. Phytotherapy Research 2000;14:210-2. 9. Boulton R, Woodman A, Calnan D, Selden C, Tam F and Hodgson H. Nonparenchymal cells from regenerating rat liver generate interleukin-1 alpha and1 beta: a mechanism of negative regulation of hepatocytes proliferation. Hepatology 1997;26:49-58. 10. Butenko IG, Gladtchenko SV and Galushko SV. Anti-inflammatory properties and inhibition of leukotriene C4 biosynthesis in vitro by flavonoid baicalein from Scutellaria baicalensis Georgy roots. Agents & Actions 1993;39:49-51. 11. Chang WH, Chen CH and Lu FJ. Different effects of baicalein, baicalin and wogonin on mitochondrial function, glutathione content and cell cycle progression in human hepatoma cell lines. Planta Med 2002;68:128-32. 12. Chen YC, Shen SC, Chen LG, Lee TJ and Yang LL. Wogonin, baicalin and baicalein inhibition of inducible nitric oxide synthase and cyclooxygenase-2 gene expressions induced by nitric oxide synthase inhibitors and lipopolysaccharide. Biochem Pharmaco 2001;61:1417-27. 13. Chen X, Nishida H and Konishi T. Baicalin promoted the repair of DNA single strand breakage caused by H2O2 in cultured NIH3T3 fibroblasts. Biol Pharm Bull 2003;26:282-4. 14. Chiu JH, Lay IS, Su MY, Chiu HL, Chiu AC, Lui WY and Wu CW. Tumor necrosis factor-producing activity of wogonin in RAW 264.7 murine macrophage cell line. Planta Med 2002;68:1036-9. 15. Cho J and Lee HK. Wogonin inhibits excitotoxic and oxidative neuronal damage in primary cultured rat cortical cells. Eur J Pharmacol 2004;485:105-10. 16. Chuang HN, Wang JY, Chiu JH, Tsai TH, Yeh SF, Fu SL, Lui WY and Wu CW. Enhancing effects of Scutellaria baicalensis and some of its constituents on TGF-beta1 gene expression in RAW 264.7 murine macrophage cell line. Planta Med 2005;71:440-5. 17. Critchfield JW, Coligan JE, Folks TM and Butera ST. Casein kinase II is a selective target of HIV-1 transcriptional inhibitors. Proc Natl Acad Sci 1997;94:6110-5. 18. Debiaggi M, Tateo F, Pagani L, Luini M and Romero E. Effects of propolis flavonoids on virus infectivity and replication. Microbiologica 1990;13:207-13. 19. Diehl AM and Rai R. Review: Regulation of liver regeneration by pro-inflammatory cytokines. J Gastroenterol Hepatol 1996;11:466-70. 20. Fausto N. Liver regeneration and repair: Hepatocytes, progenitor cells and stem cells. J Hepatol 2000;32:19-31. 21. Fenton MJ and Golenbock DT. LPS-binding proteins and receptors. J Leukoc Biol 1998;64:25-32. 22. Fishkin RJ and Winslow JT. Endotoxin-induced reduction of social investigation by mice: interaction with amphetamine and anti-inflammatory drugs. Psychopharmacology 1997;132: 335-41. 23. Gao Z, Huang K and Xu H. Protective effects of flavonoids in the roots of Scutellaria baicalensis Georgi against hydrogen peroxide-induced oxidative stress in HS-SY5Y cells. Pharmacol Res 2001;43:173-8. 24. Gao Z, Huang K, Yang X and Xu H. Free radical scavenging and antioxidant activities of flavonoids extracted from the radix of Scutellaria baicalensis Georgi. Biochimica et Biophysica Acta 1999;1472:643-50. 25. Grace LS. Review: Lipopolysaccharides in liver injury: molecular mechanisms of Kupffer cell activation. Am J Physiol Gastrointest Liver Physiol 2002;283:256-65. 26. Grace LS, Wang SC, Aminlari A, Tipoe GL, Steinstraesser L and Nanji A. Impaired hepatocyte regeneration in Toll-like receptor 4 mutant mice. Dig Dis Sci 2004;49:843-9. 27. Hajjar AM, O'Mahony DS, Ozinsky A, Underhill DM, Aderem A, Klebanoff SJ and Wilson CB. Cutting edge: functional interactions between toll-like receptor (TLR) 2 and TLR1 or TLR6 in response to phenol-soluble modulin. J Immunol 2001; 166:15-9. 28. Hayashi F, Smith KD, Ozinsky A, Hawn TR, Yi EC, Goodlett DR, Eng JK, Akira S, Underhill DM and Aderem A. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 2001;410:1099-103. 29. Hecker M, Preiss C, Schini-Kerth VB and Busse R. Antioxidants differentially affect nuclear factor kappa B-mediated nitric oxide synthase expression in vascular smooth muscle cells. FEBS Lett 1996;380:224-8. 30. Hideki O, Shuichi K, Kenichi K and Seishi M. Human hepatitis B virus enhancer 1 is responsive to human interleukin-6. J Med Virol 1997;52:413-8. 31. Hsu SL, Hsieh YC, Hsieh WC and Chou CJ. Baicalein induces a dual growth arrest by modulating multiple cell cycle regulatory molecules. Eur J Pharmacol 2001;425:165-71. 32. Hsu YH and Fu SL. Detection of endotoxin contamination in Chinese herbs by NF-κB activity-based reporter assays. Journal of Food & Drug Analysis. Journal of Food & Drug Analysis 2004;12:34-9. 33. Huang HC, Wang HR and Hsieh LM. Antiproliferative effect of baicalein, a flavonoid from a Chinese herb, on vascular smooth muscle cell. Eur J Pharmacol 1994;251:91-3. 34. Huang RL, Chen CC, Huang HL, Chang CG, Chen CF, Chang C and Hsieh MT. Anti-hepatitis B virus effects of wogonin isolated from Scutellaria baicalensis. Planta Med 2000;66:694-8. 35. Huang WH, Lee AR and Yang CH. Antioxidative and anti-inflammatory activities of polyhydroxyflavonoids of Scutellaria baicalensis Geogri. Biosci Biotechnol Biochem 2006;70:2371–80. 36. Hwang GY, Lin CY, Huang LM, Wang YH, Wang JC, Hsu CT, Yang SS and Wu CC. Detection of the hepatitis B virus X protein (HBx) antigen and anti-HBx antibodies in cases of human hepatocellular carcinoma. J Clin Microbiol 2003;41: 5598-603. 37. Isis M and Loon ND. The Golden Root: Clinical applications of Scutellaria baicalensis Georig.flavonoids as modulators of the inflammatory response. Alt Med Rev 1997;2:472-80. 38. Jesse CC, Donna WY, Douglas TG, William JC and Fabian G. Toll-like receptor-4 mediates lipopolysaccharide-induced signal transduction. J Biol Chem 1999;274:10689-93. 39. Kakumu S, Fukatsu A, Shinagawa T, Kurokawa S and Kusakabe A. Localisation of intrahepatic interleukin 6 in patients with acute and chronic liver disease. J Clin Pathol 1992;45:408–11. 40. Kakumu S, Shinagawa T, Ishikawa T, Yoshioka K, Wakita T, Ito Y, Takayanagi M and Ida N. Serum interleukin 6 levels in patients with chronic hepatitis B. Am J Gastroenterol 1991;86:1804-8. 41. Kim HM, Moon EJ, Li E, Kim KM, Nam SY and Chung CK. The nitric oxide-producing activities of Scutellaria baicalensis. Toxicology 1999;135:109-15. 42. Kim WH, Hong F, Jarugal B, Zhang Z, Fan S, Liang TJ and Gao B. Hepatitis B virus X protein sensitizes primary mouse hepatocytes to ethanol- and TNF-α-induced apoptosis by a caspase-3-dependent mechanism. Cellular & Molecular Immunology 2005;2:40-8. 43. Kimura Y, Yokoi K, Matsushita N and Okuda H. Effects of flavonoids isolated from scutellariae radix on the production of tissue-type plasminogen activator and plasminogen activator inhibitor-1 induced by thrombin and thrombin receptor agonist peptide in cultured human umbilical vein endothelial cells. J Pharm Sci 1997;49:816-22. 44. Koike K. Hepatitis B virus HBx gene and hepatocarcinogenesis. Intervirology 1995;38:134-42. 45. Krakauer T, Li BQ and Young HA. The flavonoid baicalin inhibits superantigen-induced inflammatory cytokines and chemokines. FEBS Lett 2001;500:52-5. 46. Kubo M, Kimura Y, Odani T, Tani T and Namba K. Studies on Scutellaria radix. II: The antibacterial substance. Planta Med 1981;43:194-201. 47. Kyprianou N and Isaacs JT. Expression of transforming growth factor-beta in the rat ventral prostate during castration-induced programmed cell death. Mol Endocrinol 1989;3:1515-22. 48. Lam TL, Lam ML, Au TK, Ip DT, Ng TB, Fong WP and Wan DC. A comparison of human immunodeficiency virus type-1 protease inhibition activities by the aqueous and methanol extracts of Chinese medicinal herbs. Life Sci 2000;67:2889-96. 49. Lapierre P, Beland K, Djilali-Saiah I and Alvarez F. Type 2 autoimmune hepatitis murine model:the influence of genetic background in disease development. J Autoimmun 2006;26:82-9. 50. Lee BH, Lee SJ, Kang TH, Kim DH, Sohn DH, Ko GI and Kim YC. Baicalein: an in vitro antigenotoxic compound from Scutellaria baicalensis. Planta Med 2000;66:70-1. 51. Lee GH, Nomura K and Kitagawa. Comparative study of diethylnitrosamine-initiated two-stage hepatocarcinogenesis in C3H, C57BL and BALB mice promoted by various hepatopromoters. Carcinogenesis 1989;10:2227-30. 52. Lee YH and Yun Y. HBx protein of hepatitis B virus activates JAK1-STAT signaling. J Biol Chem 1998;273:25510-5. 53. Lemaitre B, Nicolas E, Michaut L, Reichhart JM and Hoffmann JA. The dorsoventral regulatory gene cassette spatzle/Toll/cactus controls the potent antifungal response in Drosophila adults. Cell 1996;86:973-83. 54. Li BQ, Fu T, Dongyan Y, Mikovits JA, Ruscetti FW and Wang JM. Flavonoid baicalin inhibits HIV-1 infection at the level of viral entry. Biochem Biophys Res Commun 2000;276:534-8. 55. Lin CC and Shieh DE. The anti-inflammatory activity of Scutellaria rivularis extracts and its active compounds, baicalin, baicalein and wogonin. Am J Chinese Med 1996;24:31-6. 56. Lin CC, Shieh DE and Yen MH. Hepatoprotective effect of the fractions of Ban-zhi-lian on experimental liver injuries in rats. J Ethnopharmacol 1997;56:193-200. 57. (a) Lin CM, Chang H, Chen YH, Wu IH and Chiu JH. Protective role of wogonin against lipopolysaccharide-induced angiogenesis via VEGFR-2, not VEGFR-1. Int Immunopharmacol 2006;6:1690-8. 58. (b) Lin CM, Chang H, Chen YH, Wu IH and Chiu JH. Wogonin inhibits IL-6-induced angiogenesis via down-regulation of VEGF and VEGFR-1, not VEGFR-2. Planta Med 2006;72:1305-10. 59. Liu IX, Durham DG and Richards RM. Baicalin synergy with beta-lactam antibiotics against methicillin-resistant Staphylococcus aureus and other beta-lactam-resistant strains of S. aureus. J Pharm Pharmacol 2000;52:361-6. 60. Liu JJ, Huang TS, Cheng WF and Lu FJ. Baicalein and baicalin are potent inhibitors of angiogenesis: Inhibition of endothelial cell proliferation, migration and differentiation. Int J Cancer 2003;106:559-65. 61. Luedde T and Trautwein C. Intracellular survival pathways in the liver. Liver International 2006;26:1163–74. 62. Lynn WA and Golenbock DT. Lipopolysaccharide antagonists. Immunol Today 1992;13:271-6. 63. Ma SC, Du J, But PP, Deng XL, Zhang YW, Ooi VE, Xu HX, Lee SH and Lee SF. Antiviral Chinese medicinal herbs against respiratory syncytial virus. J Ethnopharmacol 2002;79:205-11. 64. Masui T, Nakanishi H, Inada K, Imai T, Mizoguchi Y, Yada H, Futakuchi M, Shirai T and Tatematsu M. Highly metastatic hepatocellular carcinomas induced in male F344 rats treated with N-nitrosomorpholine in combination with other hepatocarcinogens show a high incidence of p53 gene mutations along with altered mRNA expression of tumor-related genes. Cancer Lett 1997;112:33–45. 65. Matsumoto M, Kikkawa S, Kohase M, Miyake K and Seya T. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double-stranded RNA-mediated signaling. Biochem Biophys Res Commun 2002; 293:1364-9. 66. Matsumura T. Hayashi H. Takii T. Thorn CF. Whitehead AS. Inoue J and Onozaki K. TGF-beta down-regulates IL-1alpha-induced TLR2 expression in murine hepatocytes. J Leukoc Biol 2004;75:1056-61. 67. Medzhitov R, Preston-Hurlburt P and Janeway CA. A human homologue of the Drosophila Toll protein signals activation of adaptive immunity. Nature 1997;388:394-7. 68. Mehal WZ, Juedes AE and Crispe IN. Selective retention of actived CD8+ T cells by the normal liver. J Immunol 1999;163:3202-10. 69. Meijer C, Wiezer MJ, Diehl AM, Schouten HJ, Meijer S, van Rooijen N, van Lambalgen AA, Dijkstra CD and Van Leeuwen PA. Kepffer cell depletion by CI2MDP-liposomes alters hepatic cytokine expression and delays liver regeneration after partial hepatectomy. Liver 2000;20:66-77. 70. Nagai T, Miyaichi Y, Tomimori T, Suzuki Y and Yamada H. In vivo anti-influenza virus activity of plant flavonoids possessing inhibitory activity for influenza virus sialidase. Antiviral Research 1992;19:207-17. 71. Nagai T, Miyaichi Y, Tomimori T, Suzuki Y and Yamada H. Inhibition of influenza virus sialidase and anti-influenza virus activity by plant flavonoids. Chemical & Pharmaceutical Bulletin 1990;38:1329-32. 72. (a) Nagai T, Moriguchi R, Suzuki Y, Tomimori T and Yamada H. Mode of action of the anti-influenza virus activity of plant flavonoid, 5,7,4'-trihydroxy-8-methoxyflavone, from the roots of Scutellaria baicalensis. Antiviral Research 1995;26:11-25. 73. (b) Nagai T, Suzuki Y, Tomimori T and Yamada H. Antiviral activity of plant flavonoid, 5,7,4'-trihydroxy-8-methoxyflavone, from the roots of Scutellaria baicalensis against influenza A (H3N2) and B viruses. Biological & Pharmaceutical Bulletin 1995;18: 295-9. 74. Nan JX, Park EJ, Kim YC, Ko G and Sohn DH. Scutellaria baicalensis inhibits liver fibrosis induced by bile duct ligation or carbon tetrachloride in rats. J Pharm Pharmacol 2002;54:555-63. 75. Neuman MG. Apoptosis in diseases of the liver. Crit Rev Clin Lab Sci 2001;38:109–66. 76. Ng TB, Ling JM, Wang ZT, Cai JN and Xu GJ. Examination of coumarins, flavonoids and polysaccharopeptide for antibacterial activity. General Pharmacology 1996;27: 1237-40. 77. Olynyk JK and Clarke SL. Iron overload impairs pro-inflammatory cytokine responses by Kupffer cells. J Gastroenterol Hepatol 2001;16:438-44. 78. Ono K, Nakane H, Fukushima M, Chermann JC and Barre-Sinoussi F. Differential inhibitory effects of various flavonoids on the activities of reverse transcriptase and cellular DNA and RNA polymerases. Eur J Biochem 1990;190:469-76. 79. Onozawa M, Fukuda K, Ohtani M, Akaza H, Sugimura T and Wakabayashi K. Effects of soybean isoflavones on cell growth and apoptosis of the human prostatic cancer cell line LNCaP. Japanese Journal of Clinical Oncology 1998;28:360-3. 80. Park BK, Heo MY, Park H and Kim HP. Inhibition of TPA-induced cyclooxygenase-2 expression and skin inflammation in mice by wogonin, a plant flavone from Scutellaria radix. Eur J Pharmacol 2001;425:153-7. 81. Poltorak A, He X, Smirnova I, Liu MY, van Huffel C, Du X, Birdwell D, Alejos E, Silva M, Galanos C, Freudenberg M, Ricciardi-Castagnoli P, Layton B and Beutler B. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science 1998;282:2085-8. 82. Prost S, Ford JM, Taylor C, Doig J and Harrison DJ. Hepatitis B x protein inhibits p53-dependent DNA repair in primary mouse hepatocytes. J Biol Chem 1998;273(50):33327-32. 83. Rhee JK, Woo KJ, Baek BK and Ahn BJ. Screening of the wormicidal Chinese raw drugs on Clonorchis sinensis. Am J Chinese Med 1981;9:277-84. 84. Salgueiro JB, Ardenghi P, Dias M, Ferreira MB, Izquierdo I and Medina JH. Anxiolytic natural and synthetic flavonoid ligands of the central benzodiazepine receptor have no effect on memory tasks in rats. Pharmacol Biochem Behav 1997;58:887-91. 85. Sanchez A, Alvarez AM, Benito M and Fabregat. Apoptosis induced by transforming growth factor-β in fetal hepatocyte primary culture. J Biol Chem 1996;271:7416-22. 86. Streetz K, Leifeld L, Grundmann D, Ramakers J, Eckert K, Spengler U, Brenner D, Manns M and Trautwein C. Tumor necrosis factor α in the pathogenesis of human and murine fulminant hepatic failure. Gastroenterology 2000;119:446-60. 87. Su F and Schneider RJ. Hepatitis B virus HBx protein sensitizes cells to apoptotic killing by tumor necrosis factor α. Proc Natl Acad Sci 1997;94:8744-9. 88. Takeishi T, Hirano K, Kobayashi T, Hasegawa G, Hatakeyama K and Naito M. The role of Kupffer cells in liver regeneration. Arch Histol Cytol 1999;62:413-22. 89. Takizawa H, DelliPizzi AM and Nasjletti A. Prostaglandin I2 contributes to the vasodepressor effect of baicalein in hypertensive rats. Hypertension 1998;31:866-71. 90. Tarla MR, Ramalho FS, Ramalho LN, Silva TC, Brandao DF, Ferreira J ,Silva OC and Zucoloto S. A molecular view of liver regeneration. Acta Cirurgica Brasileira 2006;21:58-62. 91. Teramoto K, Nakamoto Y, Kunitomo T, Shoji H, Tani T, Hanazawa K and Kodama M. Removal of endotoxin in blood by polymyxin B immobilized polystyrene-derivative fiber. Ther Apher 2002;6:103-8. 92. Tralhao JG, Roudier J, Morosan S, Giannini C, Tu H, Goulenok C, Carnot F, Zavala F, Joulin V, Kremsdorf D and Brechot C. Paracrine in vivo inhibitory effects of hepatitis B virus X protein (HBx) on liver cell proliferation: An alternative mechanism of HBx-related pathogenesis. PNAS 2002;99:6991-6. 93. Tomita K, Tamiya G, Ando S, Ohsumi K, Chiyo T, Mizutani A, Kitamura N, Toda K, Kaneko T, Horie Y, Han JY, Kato S, Shimoda M, Oike Y, Tomizawa M, Makino S, Ohkura T, Saito H, Kumagai N, Nagata H, Ishii H and Hibi T. Tumor necrosis factor α signalling through activation of Kupffer cells plays an essential role in liver fibrosis of non-alcoholic steatohepatitis in mice. Gut 2006;55:415–24. 94. Uesugi T, Froh M, Arteel GE, Bradford BU and Thurman RG. Toll-like receptor 4 is involved in the mechanism of early alcohol-induced liver injury in mice. Hepatology 2001;34:101-8. 95. Ullrich SJ, Zeng ZZ and Jay G. Transgenic mouse models of human gastric and hepatic carcinomas. Semin Cancer Biol 1994;5:61-8. 96. Villar IC, Jimenez R, Galisteo M, Garcia-Saura MF, Zarzuelo A and Duarte J. Effects of chronic chrysin treatment in spontaneously hypertensive rats. Planta Med 2002;68:847-50. 97. Wakabayashi I. Inhibitory effects of baicalein and wogonin on lipopolysaccharide-induced nitric oxide production in macrophages. Pharmacology & Toxicology 1999;84:288-91. 98. Wang HK, Xia Y, Yang ZY, Natschke SL and Lee KH. Recent advances in the discovery and development of flavonoids and their analogues as antitumor and anti-HIV agents. Adv Exp Med Biol 1998;439:191-225. 99. Wang JY, Chiu JH, Tsai TH, Tsou AP, Hu CP, Chi CW, Yeh SF, Lui WY, Wu CW and Chou CK. Gene expression profiling predicts liver responses to a herbal remedy after partial hepatectomy in mice. Int J Mol Med 2005;16:221-31. 100. Wang JY, Chuang HN, Chiu JH, Fu SL, Tasi TH, Tsou AP, Hu CP, Chi CW, Yeh SF, Lui WY, Wu CW and Chou CK. Effect of Scutellaria baicalensis Georgi on macrophage-hepatocyte interaction through cytokines related to growth control of murine hepatocytes. Exp Biol Med 2006;231:444-55. 101. Wang XW, Gibson MK, Vermeulen W, Yeh H, Forrester K, Sturzbecher HW, Hoeijmarkers JHJ and Harris CC. Abrogation of p53-induced apoptosis by the hepatitis B virus X gene. Cancer Res 1995;55:6012-6. 102. Wang XW, Hussain SP, Huo TI, Wu CG, Forgues M, Hofseth LJ, Brechot C and Harris CC. Molecular pathogenesis of human hepatocellular carcinoma. Toxicology 2002;181:43-7. 103. Weil R, Sirma H, Giannini C, Kremsdorf D, Bessia C, Dargemont C, Brechot C and Israel A. Direct association and nuclear import of the hepatitis B virus X protein with the NF-κB inhibitor IκBα. Mol Cell Biol 1999;19:6345-54. 104. Wu JA, Attele AS, Zhang L and Yuan CS. Anti-HIV activity of medicinal herbs usage and potential development. Am J Chin Med 2001;29:69-81. 105. Xu KJ, Wang YH, Hu JR, Wu LH and Sun KX. Pharmacodynamics and clinical therapeutic effects of aerosol and injection of shuanghuanglian. J Chinese Materia Med 1994;19:689-92. 106. Yabu Y, Nose M, Koide T, Ohta N and Ogihara Y. Antitrypanosomal effects of traditional Chinese herbal medicines on bloodstream forms of Trypanosoma brucei rhodesiense in vitro. Southeast Asian J Trop Med Public Health 1998;29:599-604. 107. Yue J and Mulder KM. Transforming growth factor-beta signal transduction in epithelial cells. Pharmacol Ther 2001;91:1-34. 108. Zhang DY, Wu J, Ye F, Xue L, Jiang S, Yi J, Zhang W, Wei H, Sung M, Wang W and Li X. Inhibition of cancer cell proliferation and prostaglandin E2 synthesis by Scutellaria baicalensis. Cancer Res 2003;63:4037-43. 109. 呂俠卿,中藥鑑別大全,中華人民共和國湖南科學技術出版社,2002。 110. 行政院衛生署統計處,民國94年衛生統計系列死因統計,行政院衛生署出版,2006;上冊:p7-41。 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29801 | - |
dc.description.abstract | 中草藥黃芩 (Scutellaria baicalensis Georgi) 在傳統醫療的應用上常被使用於肝病治療的藥方之一。過去研究發現黃芩萃取物會影響肝臟細胞的生長調控。因此,本研究目的在探討黃芩萃取物對不同品系如C57BL/6J與C3H/HeN小鼠肝細胞之作用,且建立了一個肝細胞與庫弗氏細胞交互作用的模型,來探討Toll-like receptor 4 (TLR4)突變與HBx基因轉殖小鼠中,黃芩及不同的細胞激素在此交互作用模型中的影響。
研究方法以黃芩萃取物分別處理肝細胞與庫弗氏細胞並利用MTT與trypan blue exclusion assay觀察細胞生長情形。也藉由免疫染色來了解其作用可能的路徑;在肝細胞與庫弗氏細胞共同培養模式中,使用ELISA分析細胞激素的表現。另外,我們利用肝細胞與庫弗氏細胞交叉培養模式,在不同小鼠品系中,加入黃芩萃取物與細胞激素來了解其相互作用關係。實驗結果發現,黃芩萃取物在3 mg/ml~30 mg/ml的劑量下對C57BL/6J與C3H/HeN品系小鼠之肝細胞有時間及劑量效應地促進生長作用;而在TLR4 mutant小鼠肝細胞則有降低生長之現象。當肝細胞與庫弗氏細胞共同培養於30 mg/ml的黃芩萃取物時,初代庫弗氏細胞會抑制經由黃芩引起的肝細胞生長,其機轉可能是透過TGF-β1的分泌。在5 ng/ml的TNF-α和5 ng/ml 的IL-6協同作用下會促進wild-type品系小鼠初代肝細胞的生長;而在Hbx 基因轉殖小鼠肝細胞中,無法看到促進肝細胞生長之現象。 因此,本研究結論認為黃芩萃取物對不同品系如C57BL/6J與C3H/HeN之促進肝細胞生長沒有差異。但是對於TLR4 wild-type與mutant小鼠之肝細胞生長有差異,TLR4在黃芩促進肝細胞生長中扮演了部分的角色。在肝細胞與庫弗氏細胞共同培養方面,黃芩萃取物30 mg/ml濃度下會促使庫弗氏細胞生長,並分泌TGF-β1來抑制肝細胞的生長。而庫弗氏細胞對肝細胞的抑制生長作用與TLR4無相關。在細胞激素的影響方面,細胞激素TNF-α和IL-6可以促進肝細胞生長,但並不透過TLR4的路徑。 | zh_TW |
dc.description.abstract | Scutellaria baicalensis Georgi (SbG) is a well-known herbal medicine commonly used for the treatment of human liver disease. Previous investigation showed that SbG affected the proliferation of the liver cell. The aim of this study was to elucidate the effects of SbG on hepatocyte growth in different strains mice, such as C57BL/6J and C3H/HeN, and to establish a hepatocyte-Kupffer cell interaction model for analyzing the influence of SbG ,TNF-αand IL-6 in the Toll-like receptor 4 (TLR4)-mutant and HBx-transgenic mice.
The effects of SbG, TNF-α and IL-6 on hepatocyte proliferation in different strains mice were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide and trypan blue exclusion assay. Cytokine production and antibody-neutralization studies were used on SbG-treated hepatocyte-Kupffer cell interaction model. The results showed that SbG stimulated the proliferation of the primary hepatocytes in a dose-dependent 3 mg/ml~30 mg/ml manner. However, the TLR4 mutant mice have less cell growth-stimulation effects. The SbG-stimulated proliferation was inhibited by TGF-β1 secretion from primary Kupffer cells. The synergistic effect of TNF-α and IL-6 induced the hepatocyte growth in the wild-type mice;whereas such effect was negative in the HBx-transgenic mice. It was concluded that there was no significant difference in terms of SbG-induced hepatocyte proliferation between different strains mice, such as C57BL/6J and C3H/HeN. However, it has significant difference between TLR4 wild-type and TLR4 mutant mice. Hence, TLR4 receptor might play a partial role in the SbG-induced hepatocyte proliferation. In the coculture model, TGF-β1 inhibit the SbG-induced hepatocyte proliferation at the dose of 30 mg/ml. TLR4 pathway was not involved in TNF-α- and IL-6-stimulated hepatocyte proliferation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T01:19:26Z (GMT). No. of bitstreams: 1 ntu-96-R91629018-1.pdf: 981948 bytes, checksum: 568066b2a3bc78e4be1ffd0839cc1b74 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 目次
口試委員會審定書..................................Ⅰ 誌謝............................................. Ⅱ 中文摘要..........................................Ⅲ 英文摘要..........................................Ⅳ 目次..............................................Ⅴ 表次. ............................................Ⅵ 圖次..............................................Ⅶ 第一章、緒言.......................................1 第二章、文獻探討 第一節、肝臟生理與肝細胞生長之調控 一、肝臟的解剖與生理...............................5 二、肝細胞生長之調控...............................6 三、庫弗氏細胞在肝細胞生長調控中所扮演的角色.......7 第二節、黃芩 一、黃芩簡介.......................................9 二、黃芩的現代藥理作用........................... 10 三、過去研究黃芩對小鼠肝細胞與庫弗氏細胞交互作用之影響................................................15 第三節、實驗動物 一、C3H小鼠品系...................................16 二、Hbx基因轉殖小鼠.............................. 17 三、不同品系對疾病發生之影響......................18 第三章、研究目的................................. 19 第四章、材料與方法 第一節、實驗材料 一、黃芩..........................................22 二、實驗動物......................................22 三、儀器設備......................................23 四、試劑................................... ......24 第二節、初代細胞之分離與培養 一、分離與培養..................................29 (一) 初代肝細胞 (二) 初代庫弗氏細胞 二、分流式細胞分析................................30 三、初代肝細胞與庫弗氏細胞共同培養模式之建立..... 30 第三節、黃芩對初代肝細胞與庫弗氏細胞生長之測量 一、Trypan blue exclusion assay.................31 二、MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay)................32 第四節、探討黃芩對初代肝細胞與庫弗氏細胞作用機制之研究法 一、細胞免疫螢光染色法.. .......................32 二、細胞介質分析........ .......................33 三、抗體中和反應...... .........................34 第五節、不同品系小鼠TLR-4基因之確認 一、Gemonic DNA之萃取...........................34 二、聚合酵素鏈鎖反應(PCR;polymerase chain reaction)..35 三、DNA定序.....................................36 第六節、黃芩對不同品系小鼠肝細胞與庫弗氏細胞交叉培養試驗................................................37 第七節、TNF-α與IL-6對不同品系小鼠肝細胞與庫弗氏細胞交叉培養試驗. ............................................38 第八節、實驗設計. ................................39 第九節、統計方法. ................................39 第五章、結果 第一節、黃芩、細胞與動物之確認................41 一、高效液相層析技術(HPLC)分析..............41 二、初代庫弗氏細胞之確認....................41 三、C3H/HeN與C3H/HeJ小鼠TLR4基因之聚合酵素連鎖反應與定序................................................41 第二節、黃芩對初代培養之作用 一、黃芩對初代肝細胞生長之劑量與時間效應............42 二、黃芩對初代庫弗氏細胞之作用................42 三、黃芩誘發初代肝細胞中NF-κB之表現...........43 第三節、肝細胞與庫弗氏細胞共同培養模式 一、黃芩對共同培養模式中肝細胞生長之劑量效應........43 二、黃芩誘導共同培養模式中TGF-β1之生成與影響..44 第四節、黃芩對不同品系小鼠之影響 一、黃芩對不同品系小鼠初代肝細胞生長之作用 ...45 二、黃芩對不同品系小鼠肝細胞與庫弗氏細胞交叉培養試驗. ................................................45 第五節、TNF-α與IL-6對不同品系小鼠之影響 一、不同濃度之TNF-α與IL-6對初代肝細胞生長之作用.....46 二、TNF-α與IL-6透過TLR4路徑在肝細胞與庫弗氏細胞交 叉培養試驗中對肝細胞生長之作用. .................47 三、TNF-α與IL-6在Hbx基因轉殖小鼠中對肝細胞與庫弗氏 細胞交叉培養試驗中肝細胞生長之作用. ................47 第六章、討論 第一節、實驗方法與模式建立之討論 一、黃芩中LPS的污染問題.......................50 二、初代細胞培養之必要性.... .......................50 三、肝細胞與庫弗氏細胞共同培養模式之建立......51 四、不同品系細胞交叉試驗中細胞間排斥情形之探討..51 第二節、實驗結果之討論 一、黃芩對初代肝細胞之作用與機制..............53 二、黃芩對肝細胞與庫弗氏細胞共同培養模式的作用機制..53 三、黃芩透過TLR4路徑對肝細胞之作用. ...........54 四、TLR4在黃芩對肝細胞與庫弗氏細胞交叉培養中之角色.55 五、TNF-α與IL-6對不同品系小鼠肝細胞與庫弗氏細胞交叉培養試驗..............................................56 第三節、未來展望..................................58 第七章、結論........................................59 參考文獻. ..........................................61 圖表.... ...........................................76 附錄.. ............................................100 | |
dc.language.iso | zh-TW | |
dc.title | 黃芩對不同品系小鼠肝細胞與庫弗氏細胞交互作用之影響 | zh_TW |
dc.title | Effects of Scutellaria baicalensis Georgi on hepatocyte and Kupffer cell in different strains mice | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 邱仁輝教授(Jen-Hwey Chiu) | |
dc.contributor.oralexamcommittee | 戚謹文教授 | |
dc.subject.keyword | 黃芩,肝細胞,庫弗氏細胞,小鼠品系, | zh_TW |
dc.subject.keyword | SbG,hepatocyte,kupffer cell,mice strain, | en |
dc.relation.page | 99 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-19 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-96-1.pdf 目前未授權公開取用 | 958.93 kB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。