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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29795
標題: | 應用多光子螢光及二倍頻顯微術於肝細胞癌診斷 Application of Multiphoton Fluorescence and Second Harmonic Generation Microscopy in Diagnosing Hepatocellular Carcinoma |
作者: | Tzu-Lin Sun 孫梓凌 |
指導教授: | 董成淵(Chen-Yuan Dong) |
關鍵字: | 雙光子螢光,二倍頻,肝細胞癌, two-photon fluorescence microscopy (TPFM),second harmonic generation (SHG),hepatocellular carcinoma (HCC), |
出版年 : | 2007 |
學位: | 碩士 |
摘要: | 肝癌長久以來一直是國人揮之不去夢魘,而國內對於肝癌的研究也是相當重視。但是醫師對於癌症判斷,卻僅止於經驗法則,雖然此法已行之有年,但主觀上判讀仍是存在精確度的疑慮。若有更客觀的方式,去建立一套分析的準則,不僅可以提高癌症級數判讀的準確度,同樣也是輔助醫師的一項利器。
而本實驗室便是利用雙光子螢光與二倍頻顯微術(以下簡稱顯微術)來觀測肝細胞癌(HCC)切片,藉此達到上述之目的。藉著本實驗室顯微術系統,掃描各級數是切片樣本,在擷取其螢光與二倍頻影像,再進一步分析。 將取得之影像對其雙光子螢光或是二倍頻訊號強度,與各種參數分析,試著去找出其中的對應關係與趨勢。而利用這些結果去建立一套較客觀的準則,藉此輔助或是精進癌症程度的判斷。 而目前所得結果顯示,就自發螢光而言,非癌症樣品較癌症樣品高,但仍需較多的樣本數來驗證。並試著藉此結論建立分級標準。 Hepatic disease has always been a major health problem in Taiwan, with hepatocellular carcinoma (HCC) being among the leading causes of death. Since the diagnosis of the degree of liver carcinoma is done according to the doctor’s clinical experience, there is a need for a more objective determination of the extent of this cancer. In this work, we attempt to develop an objective method to help improve the accuracy of HCC classification which will be a useful aid for clinical investigations. In this study, we make use of two-photon fluorescence (TPF) and second harmonic generation (SHG) microscopy in imaging hepatocellular carcinoma (HCC) specimens. By scanning thin sections of different HCC specimens, we attempt to correlate the change in the sample’s autofluorescence with parameters such as tumor size. We hope that this approach can lead to a more accurate classification of HCC. Our preliminary results show that the autoflurescence intensity of non-tumor sample is higher than that of tumor sample. Our approach demonstrates the possibility of using tissue autofluroescence for tumor classification and the potential of this methodology for clinical diagnosis. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29795 |
全文授權: | 有償授權 |
顯示於系所單位: | 物理學系 |
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