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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝旭亮 潘素美 | |
dc.contributor.author | Ing-Chien Chen | en |
dc.contributor.author | 陳英謙 | zh_TW |
dc.date.accessioned | 2021-06-13T01:18:26Z | - |
dc.date.available | 2007-07-26 | |
dc.date.copyright | 2007-07-26 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-17 | |
dc.identifier.citation | I
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29777 | - |
dc.description.abstract | 本論文探討三個參與光線訊息傳遞分子的功能,這三個分子為:綠豆VrGIR1 (gibberellic acid [GA]- and IR [infrared]-induced gene 1)、阿拉伯芥AtGASA4 (gibberellic acid-stimulated transcripts in Arabidopsis) 與 AtFIP1 (FIN219[far-red insensitive 219]-interacting protein 1, AtGSTU20)。
在第一部分研究中,由差異表現法篩選到2-5 | zh_TW |
dc.description.abstract | The functions of three light signaling components, VrGIR1 (gibberellic acid [GA]- and IR [infrared]-induced gene 1) in mungbean (Vigna radiata), AtGASA4 (gibberellic acid-stimulated transcripts in Arabidopsis) and AtFIP1 (FIN219[far-red insensitive 219]-interacting protein 1, AtGSTU20) in Arabidopsis (Arabidopsis thaliana) were characterized in this study.
In part I, VrGIR1 was isolated by differential display from mungbean seedlings irradiated with 2 to 5 | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T01:18:26Z (GMT). No. of bitstreams: 1 ntu-96-D87226003-1.pdf: 3159528 bytes, checksum: 938b2b882f500b56923cf01e86a4c21b (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 誌謝……………………………………………………………………… i
目錄……………………………………………………………………… ii PART I: Both VrGIR1 and AtGASA4, GA- and IR-Stimulated Genes, Participate in Light Signaling in Mungbean and Arabidopsis 中文摘要…………….…………………………………………………… 2 Abstract…………………………………………………………………… 3 Introduction…………………………………………………………….. 4 Materials and methods………………………………………………… 10 Plant Material and Light Treatment………………………………..... 10 RNA Extraction, RNA Gel Blot and RT-PCR Analyses…………….. 10 mRNA Differential Display System………………………………… 11 Complementation Test of the gasa4 Mutant………………………… 12 Response of the gasa4 T-DNA Inserted Mutant to Exogenous Application of GA………………………………………………… 13 Determination of Flowering Time for the gasa4 T-DNA Inserted Line………………………………………………………………… 13 Results………………………………………………………………….. 14 Isolation of IR-Induced Genes VrGIR1 from Mungbean……………. 14 VrGIR1 Is Induced by GA and Displayed Tissue Specific Expression………………………………………………………… 15 VrGIR1 Is an AtGASA-Related Protein……………………………… 16 Expressions of VrGIR1 and AtGASA4 Are Differentially Regulated by Light…………………………………………………………… 17 AtGASA4 Is Regulated by Photoreceptors and Light Signaling Transducers……………………………………………………… 18 The Null Mutant of gasa4 Exhibits a Hyposensitive Phenotype under R and FR Light……………………………………………. 19 The Null Mutant of gasa4 Can Be Rescued by the Ectopic Expressions of AtGASA4……………………………………………. 19 AtGASA4 Represses Several Key Enzyme-Corresponding Genes Involved in GA Metabolism……………………………………… 20 The gasa4 Mutant Exhibits Increased Response to Exogenous GA... 21 The gasa4 Mutant Shows an Earlier Flowering Phenotype under Long-Day Conditions……………………………………………… 22 Discussion……………………………………………………………….. 24 Table………………………………………………………………………. 33 Figures…………………………………………………………………… 34 References………………………………………………………………… 53 Appendix……………………………………………………………….. 60 PART II: Glutathione S-Transferase Interacting with FIN219 Is Involved in Phytochrome A-Mediated Signaling in Arabidopsis 中文摘要………………………………………………………………… 62 Abstract………………………………………………………………….. 63 Introduction……………………………………………………………… 64 Materials and Methods………………………………………………….. 69 Plant Materials and Growth Conditions…………………………….. 69 Yeast Two-Hybrid Assay…………………………………………….. 70 Pull-Down Assay…………………………………………………….. 71 Protein Co-Immunoprecipitation……………………………………… 73 Recombinant Plasmids for Plant Transformation…………………….. 73 Glutathione S-Transferase Activity Assay……………………………. 74 RNA Gel Blot and RT-PCR Analyses……………………………….. 75 Results…………………………………………………………………… 77 Isolation of FIN219-Interacting Partners by Yeast Two-Hybrid Method…………………………………………………………….. 77 FIP1 Encodes a Plant Glutathione S-Transferase with Affinities on the Substrates Glutathione (GSH) and 1-Chloro-2,4- Dinitrobenzene (CDNB)…………………………………………. 79 Transgenic Seedlings Overexpressing or Showing Reduced FIP1 Expression Exhibit a Hyposensitive Phenotype under cFR……… 81 FIP1 Expression Is Regulated by Light Signaling Transducers…….. 83 Transgenic Plants Overexpressing or Reducing FIP1 Expression Displayed a Delayed Flowering Phenotype under Long-Day conditions………………………………………………………… 84 Discussion………………………………………………………………… 86 Figures……………………………………………………………………. 91 Table…………………………………………………………………….. 101 References……………………………………………………………….. 103 | |
dc.language.iso | en | |
dc.title | 綠豆與阿拉伯芥中參與光線訊息傳遞分子VrGIR1、AtGASA4與AtFIP1的功能性研究 | zh_TW |
dc.title | Functional Studies of Light Signaling Components, VrGIR1, AtGASA4 and AtFIP1, in Mungbean and Arabidopsis | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 張孟基 | |
dc.subject.keyword | 阿拉伯芥,遠紅光,FIN219,FIP1,GASA4,Gibberellin,GIR1,Glutathione S-transferase,紅外光,綠豆, | zh_TW |
dc.subject.keyword | Arabidopsis,Far-red,FIN219,FIP1,GASA4,Gibberellin,GIR1,Glutathione S-transferase,Infrared,Mungbean, | en |
dc.relation.page | 107 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-19 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
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ntu-96-1.pdf 目前未授權公開取用 | 3.09 MB | Adobe PDF |
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