請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29734完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 翁秀貞(Shiou-Jeng Ong),戴榮湘(Jung-Hsiang Tai) | |
| dc.contributor.author | Ya-Ting Wang | en |
| dc.contributor.author | 王雅婷 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:16:43Z | - |
| dc.date.available | 2009-08-08 | |
| dc.date.copyright | 2007-08-08 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-19 | |
| dc.identifier.citation | 1. Adams, B., A. Musiyenko, R. Kumar, and S. Barik. 2005. A Novel Class of Dual-family Immunophilins. J. Biol. Chem. 280:24308-24314.
2. Addis, M. F., P. Rappelli, P. Cappuccinelli, and P. L. Fiori. 1997. Extracellular release by Trichomonas vaginalis of a NADP+ dependent malic enzyme involved in pathogenicity. Microb. Pathog. 23:55-61. 3. Aggen, J. B., A. C. Nairn, and R. Chamberlin. 2000. Regulation of protein phosphatase-1. Chem Biol. 7:13-23. 4. Alderete, J. F., D. Provenzano, and M. W. Lehker. 1995. Iron mediates Trichomonas vaginalis resistance to complement lysis. Microb. Pathog. 19:93–103. 5. Alderete, J. F., J. Engbring, C. M. Lauriano, and J. L. O’Brien. 1998. Only two of the Trichomonas vaginalis triplet AP51 adhesins are regulated by iron. Microb. Pathog. 24:1-16. 6. Alderete, J. F. 1999. Iron modulates phenotypic variation and phosphorylation of P270 in double-stranded RNA virus-infected Trichomonas vaginalis. Infect. Immun. 67:4298-4302. 7. Alderete, J. F., K. W. Millsap, M. W. Lehker, and M. Benchimol. 2001. Enzymes on microbial pathogen and Trichomonas vaginalis: molecular mimicry and functional diversity. Cell Microbiol. 3:359-370. 8. Arroyo, R., J. Engbring, and J. F. Alderete. 1992. Molecular basis of host epithelial cell recognition by Trichomonas vaginalis. Mol. Microbiol. 6:853-862. 9. Bastida-Corcuera, F. D., C. Y. Okumura, A. Colocoussi, and P. J. Johnson. 2005. Trichomonas vaginalis lipophosphoglycan mutants have reduced adherence and cytotoxicity to human ectocervical cells. Eukaryot. Cell 4:1951–1958. 10. Bell, A., P. Monaghan, and A. P. Page. 2006. Peptidyl-prolyl cis–trans isomerases (immunophilins) and their roles in parasite biochemistry, host–parasite interaction and antiparasitic drug action. Int. J. Parasitol. 36:261–276. 11. Blisnick, T., L. Vincensini, G. Fall, and C. Braun-Breton. 2006. Protein phosphatase 1, a Plasmodium falciparum essential enzyme, is exported to the host cell and implicated in the release of infectious merozoites. Cell Microbiol. 8:591-601. 12. Bollen, M. 2001. Combinatorial control of protein phosphatase-1. Trends Biochem. Sci. 26:426-431. 13. Boudreau, R. T., and D. W. Hoskin. 2005. The use of okadaic acid to elucidate the intracellular role(s) of protein phosphatase 2A: Lessons from the mast cell model system. Int Immunopharmacol. 5:1507–1518. 14. Chou, C. F., and J. H. Tai. 1996. Simultaneous extraction of DNA and RNA from nuclease-rich pathogenic protozoan Trichomonas vaginalis. BioTechniques. 20:790-791. 15. Dolezal, P., S. Vanacova, J. Tachezy, and I. Hrdy. 2004. Malic enzymes of Trichomonas vaginalis: two enzyme families, two distinct origins. Gene. 329:81-92. 16. Dornan, J., A. P. Page, P. Taylor, S. Y. Wu, A. D. Winter, H. Husi, and M. D. Walkinshaw. 1999. Biochemical and structural characterization of a divergent loop cyclophilin from Caenorhabditis elegans. J. Biol. Chem. 274:34877-34883. 17. Egloff, M. P., D. F. Johnson, G. Moorhead, P. T. Cohen, P. Cohen, and D. Barford. 1997. Structural basis for the recognition of regulatory subunits by the catalytic subunit of protein phosphatase 1. EMBO J. 16:1876-1887. 18. Engbring, J. A., and J. F. Alderete. 1998. Three genes encode distinct AP33 proteins involved in Trichomonas vaginalis cytoadherence. Mol. Microbiol. 28:305-313. 19. Frangioni J. V., and B. G. Neel. 1993. Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion protein. Anal. Biochem. 210:179-187. 20. Garcia, A. F., T. H. Chang, M. Benchimol, D. J. Klumpp, M. W. Lehker, and J. F. Alderete. 2003. Iron and contact with host cells induce expression of adhesions on surface of Trichomonas vaginalis. Mol Microbiol. 47:1207-1224. 21. Gothel, S. F., and M. A. Marahiel. 1999. Peptidyl-prolyl cis-trans isomerase, a superfamily of ubiquitous folding catalysts. Cell. Mol. Life Sci. 55:423-436. 22. Granger B. L., S. J. Warwood, M. Benchimol, and W. De-Souza. 2000. Transient invagination of flagella by Tritrichomonas foetus. Parasitol Res. 86:699–709. 23. Henriksson L. M., P. Johansson, T. Unge, and S. L. Mowbray. 2004. X-ray structure of peptidyl-prolyl cis-trans isomerase A from Mycobacterium tuberculosis. Eur. J. Biochem. 271:4107-4113. 24. Hofmann K., and P. Bucher. 1995. The FHA domain: a putative nuclear signaling domain found in protein kinases and transcription factors. TIBS 20:347-349. 25. Jesus, J. B., M. A. Vannier-Santos, C. Britto, P. Godefroy, F. C. Silva-Filho, A. A. Pinheiro, B. Rocha-Azevedo, A. H. Lopes, and JR Meyer-Fernandes. 2004. Trichomonas vaginalis virulence against epithelial cells and morphological variability: the comparison between a well-established strain and a fresh isolate. Parasitol. Res. 93:369–377. 26. Ke, H., and Q. Huai. 2004. Crystal structure of cyclophilin and its parteners. Front. Biosci. 9:2285-2296. 27. Keckesova Z., L. M. Ylinen, and G. J. Towers. 2006. Cyclophilin A renders human immunodeficiency virus type 1 sensitive to old world monkey but not human TRIM5α antiviral activity. J. Virol. 80: 4683-4690. 28. Keister, D. B. 1983. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trop. Med. and Parastiol. 72:431-432. 29. Kullertz G., S. Luthe, and G. Fischer. 1998. Semiautomated microtiter plate assay for monitoring peptidylprolyl cis/trans isomerase activity in normal and pathological human sera. Clin. Chem. 44:502-508. 30. Lehker, M. W., R. Arroyo, and J. F. Alderete. 1991. The regulation by iron of the synthesis of adhesions and cytoadherence levels in the protozoan Trichomonas vaginalis. J. Exp. Med. 174:311-318. 31. Leverson J. D., and S. A. Ness. 1998. Point Mutations in v-Myb Disrupt a Cyclophilin-Catalyzed Negative Regulatory Mechanism. Mol. Cell. 1:203-211. 32. Li, J., G. I. Lee, S. R. Van Doren, and J. C. Walker. 2000. The FHA domainmediates phosphoprotein interaction. J. Cell Sci. 113:4143-4149. 33. Lu, K. P. 2004. Pinning down cell signaling, cancer and Alzheimer’s disease. Trends Biochem. Sci. 29:200-209. 34. Ma, D., L. S. Nelson, K. LeCoz, C. Poole, and C. K. Carlow. 2002. A novel cyclophilin from parasitic and free-living nematodes with a unique substrate- and drug-binding domain. J. Biol. Chem. 277:14925-14932. 35. Martin, H., M. Flandez, C. Nombela, and M. Molina. 2005. Protein phosphatases in MAPK signalling: we keep learning from yeast. Mol Microbiol. 58:6-16. 36. Mettus, R. V., J. Litvin., A. Wali, A. Toscani, K. Latham, K. Hatton, E. P. Reddy. 1994. Murine A-myb:evidence for differential splicing and tissue-specific expression. Oncogene 9:3077-3086. 37. Meza-Zepeda, L., M. Baudo, E. Palva, and P. Heino. 1998. Gene note. Isolation and characterization of a cDNA corresponding to a stress-activated cyclophilin gene in Solanum commersonii. J. Exp. Bot. 49:1451-1452. 38. Moreno-Brito V., C. Yanez-Gomez, P. Meza-Cervantez, L. Avila-Gonzalez, M. A. Rodriguez, J. Ortega-Lopez, A. Gonzalez-Robles, and R. Arroyo. 2005. A Trichomonas vaginalis 120 kDa protein with identity to hydrogenosome pyruvate:ferredoxin oxidoreductase is a surface adhesin induced by iron. Cell Microbiol. 7:245-258. 39. Mundodi, V., A. S. Kucknoor, D. J. Klumpp, T. H. Chang, and J. F. Alderete. 2004. Silencing the ap65 gene reduces adherence to vaginal epithelial cells by Trichomonas vaginalis. Mol.Microbiol. 53:1099-1108. 40. Ness, S. A., E. Kowenz-Leutz, T. Casini, T. Graf, and A. Leutz. 1993. Myb and NF-M: combinatorial activators of myeloid genes in heterologous cell types. Genes Devel. 7:749-759. 41. Ness, S. A., J. D. Engel. 1994. Vintage reds and whites: Combinatorial transcription factor utilization in hematopoietic differentiation. Curr. Opin. Genet. Devel. 4:718-724. 42. Ness, S. A. 1999. Myb binding proteins: regulators and cohorts in transformation. Oncogene 18:3039-3046. 43. Ness, S. A. 2003. Myb protein specificity: evidence of a context-specific ranscription factor code. Blood Cells Mol Dis. 31:192-200. 44. O’Brien, J. L., C. M. Lauriano, and J. F. Alderete. 1996. Molecular characterization of a third malic enzyme-like AP65 adhesin gene of Trichomonas vaginalis. Microb. pathog. 20:335-349. 45. Oh, I. H., E. P. Reddy. 1999. The myb gene family in cell growth, differentiation and apoptosis. Oncogene 18:3017-3033. 46. Ong, S. J., S. C. Huang, H. W. Liu, and J. H. Tai. 2004. Involvement of multiple DNA elements in iron-inducible transcription of the ap65-1 gene in the protozoan parasite Trichomonas vaginalis. Mol Microbiol. 52:1721-1730. 47. Ong, S. J., H. M. Hsu, H. W. Liu, C. H. Chu, and J. H. Tai. 2006. Multifarious Transcriptional Regulation of Adhesion Protein Gene ap65-1 by a Novel Myb1 Protein in the Protozoan Parasite Trichomonas vaginalis. Eukaryot. Cell 5:391–399. 48. Page, A. P., K. MacNIVEN, and M. O. Hengartner. 1996. Cloning and biochemical characterization of the cyclophilin homologues from the free-living nematode Caenorhabditis elegans. Biochem. J. 317:179-185. 49. Pearson, G., F. Robinson, G. T. Beers, B. E. Xu, M. Karandikar, K. Berman, and M. H. Cobb. 2001. Mitogen-activated protein (MAP) kinase pathways: regulation and physiological functions. Endocr Rev. 22: 153-183. 50. Pereira-Neves, A., K. C. Ribeiro, and M. Benchimol. 2003. Pseudocysts in Trichomonads- New Insight. Protist 154:313-329. 51. Petrin, D., K. Delgaty, R. Bhatt, and G. Garber. 1998. Clinical and Microbiological Aspects of Trichomonas vaginalis. Clin Microbiol Rev. 11:300-317. 52. Romano, P., J. Gray, P. Horton, and S. Luan. 2005. Plant immunophilins: functional versatility beyond protein maturation. New Phytol. 166:753-769. 53. Rosinski, J. A., W. R. Atchley. 1998. Molecular Evolution of the Myb Family of Transcription Factors: Evidence for Polyphyletic Origin. J Mol Evol. 46:74–83. 54. Schultz, J., R. R. Copley, T. Doerks, C. P. Ponting, and P. Bork. 2000. SMART: a web-based tool for the study of genetically mobile domains. Nucl. Acids Res. 28:231-234. 55. Stone J. M., M. A. Collinge, R. D. Smith, M. A. Horn, and J. C. Walker. 1994. Interaction of a protein phosphatase with an Arabidopsis serine-threonine receptor kinase. Sci. 266:793 – 795. 56. Sun, C. H., D. Palm, A. G. McArthur, S. G. Svard, and F. D. Gillin. 2002. A novel Myb-related protein involved in transcriptional activation of encystations genes in Giardia lamblia. Mol. Microbiol. 46:971-984. 57. Tai, J. H., H. M. Su, J. Tsai, M. F. Shaio, and C. C. Wang. 1993. The divergence of Trichomonas vaginalis virus RNAs among various isolates of Trichomonas vaginalis. Exp. Parasitol. 76:278-286. 58. Taylor, P., A. P. Page, G. Kontopidis, H. Husi, and M. D. Walkinshaw. 1998. The X-ray structure of a divergent cyclophilin from the nematode parasite Brugia malayi. FEBS Lett. 425:361-366. 59. Tocher, J. H., and D. I. Edwards. 1994. Evidence for the direct interaction of reduced metronidazole derivatives with DNA bases. Biochem. Pharmacol. 48:1089–1094. 60. Trauth K., B. Mutschler, N. A. Jenkins, D. J. Gilbert, N. G. Copeland, and K. H. Klempnauer. 1994. Mouse A-myb encodes a trans-activator and is expressed in mitotically active cells of the developing central nervous system, adult testis and B lymphocytes. EMBO J. 13:5994-6005. 61. Tsai, C. D., H. W. Liu, J. H. Tai. 2002. Characterization of an iron-responsive promoter in the protozoan pathogen Trichomonas vaginalis. J. Biol. Chem. 277:5153-5162. 62. Wakula, P., M. Beullens, H. Ceulemans, W. Stalmans, and M. Bollen. 2003. Degeneracy and function of the ubiquitous RVXF motif that mediates binding to Protein Phosphatase-1. J. Biol. Chem. 278:18817-18823. 63. Wang, A., C. C. Wang, and J. F. Alderete. 1987. Trichomonas vaginalis phenotypic variation occurs only among trichomonads infected with the double-stranded RNA virus. J. Exp. Med. 166:142-150. 64. Wang, P., and J. Heitman. 2005. The cyclophilins. Genome Biology 6:226. 65. Zydowsky L. D., F. A. Etzkorn, H. Y. Chang, S. B. Ferguson, L. A. Stolz, S. I. Ho, and C. T. Walsh. 1992. Active site mutants of human cyclophilin A separate peptidyl-prolyl isomerase activity from cyclosporin A binding and calcineurin inhibition. Protein Sci. 1:1092-1099. 66. 黃勝祈. 2002. 探討鐵離子級細胞生長對陰道滴蟲ap65-1啟動子的調控表現. 台灣大學微生物學研究所寄生蟲學組碩士論文. 67. 劉怡棻. 2002. 陰道滴蟲 myb4 基因之選殖及生化分析. 台灣大學微生物學研究所寄生蟲學組碩士論文. 68. 李明純. 2003. 陰道滴蟲 tvMyb3 蛋白質之生化特性分析. 台灣大學微生物學研究所寄生蟲學組碩士論文. 69. 許弘明. 2004. 陰道滴蟲tvMyb 家族蛋白質生化特性分析. 台灣大學微生物學研究所寄生蟲學組碩士論文. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29734 | - |
| dc.description.abstract | 陰道滴蟲中附著蛋白質 ap65-1 基因之表現受到鐵離子與生長環境改變而影響。前人發現鐵能促進 Myb1之核輸送，以抑制受鐵誘發之 ap65-1 轉錄活性。
前人以大腸桿菌雙雜交系統(bacterioMatch Two-Hybrid System)篩選陰道滴蟲 cDNA 基因庫，得到一與 Myb1相互作用之蛋白質，CypA-1，此 cyclophilin-like protein 為脯氨酸異構脢(peptidyl prolyl cis-trans isomerase，PPIase)蛋白質家族中 cyclophilin (Cyp)一類。本研究以 GST-pull down 分析進一步證實，Myb1與 CypA-1為專一性作用。CypA-1 mRNA 表現量因高鐵處理而提高。利用酵素活性分析得知，CypA-1酵素活性 kcat/Km 為103 M-1S-1；而突變株 R63A 酵素活性 kcat/Km 為6×102 M-1S-1。由螢光免疫分析及西方轉漬法發現，CypA-1及 R63A 皆表現於陰道滴蟲細胞質中，而 R63A 的過度表現會減低 Myb1進入細胞核。經片段刪減及單點突變得知，Myb1與 CypA-1作用區域可能為104EYGPKWNKI112。配合 GST-pull down 分析發現，突變株 YGP105-107AAA 與 Myb1作用力減弱，指出 Y105-P107參與兩者間的作用。進一步將 Myb1及其突變株轉染於陰道滴蟲細胞中，發現 Myb1表現在細胞核中，突變株 P107A 聚集成顆粒於核膜附近，突變株 YGP105-107AAA 則表現於細胞質中。以 Cyclosporin A 處理轉染株 Myb1，發現 Myb1或聚集成顆粒或均勻散布在細胞質中，顯示 CypA-1與 Myb1之作用與 Myb1核輸送功能有關。 本研究證實 Myb1與 CypA-1間的交互作用，助於了解 Myb1蛋白質之生理功能，同時也提供了 Myb1於陰道滴蟲基因調控上ㄧ新的研究方向。 | zh_TW |
| dc.description.abstract | Expression of the adhesion protein ap65-1 gene in the human pathogen, Trichomonas vaginalis, is critically regulated by iron, which was shown to enhance the nuclear translocation of Myb1 that may inhibit iron-activated transcription of the ap65-1 gene.
In this study, bacterioMatch Two-Hybrid System (Stratagene) was applied to identify potential Myb1 interaction proteins. One of Myb1 interacting partner proteins was identified, and named CypA-1. CypA-1 is a cyclophilin-like protein, a family of highly conserved ubiquitous proteins in all the organisms. Specific interaction between CypA-1 and Myb1 was confirmed by the GST-pull down assay. CypA-1 mRNA level increased with iron treatment. Recombinant CypA-1 was assayed for cis-trans peptidyl-prolyl isomerase (PPIase) activity, and gave a value of kcat/Km 103 M-1S-1. R63A retained 60 % of the catalytic efficiency (kcat/Km) of wild-type recombinant CypA-1. Based on the immunofluorescence staining and Western blot assay, both CypA-1 and R63A were expressed in the cytoplasm. Expression of R63A inhibited the nuclear translocation of Myb1. The region of Myb1 interacting with CypA-1 was found to be E104-I112. YGP105-107AAA also interacted with CypA-1 with decreased activity as reveal by the GST-pull down assay. Based on the immunofluorescence staining, Myb1 expressed in the nucleus, P107A was accumulated close to the nuclear membrane, but YGP105-107AAA was evenly distributed in the cytoplasm. Results indicate the participation by Y105-P107 in the translocation of Myb1. Myb1 was either expressed as accumulated close to nuclear membrane, or in the cytoplasm by cyclosporin A treatment. These results provide a new aspect for investigation on the role of the Myb1 protein in transcription regulation of Trichomonas vaginalis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:16:43Z (GMT). No. of bitstreams: 1 ntu-96-R93445202-1.pdf: 3220854 bytes, checksum: 5581f5ce7c49e386d943689307f73633 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目 錄
縮寫表 1 英文摘要 2 中文摘要 3 前言 4 ㄧ、陰道滴蟲之簡介 4 二、陰道滴蟲之附著機制 4 三、鐵離子與陰道滴蟲之關係 5 四、Myb 轉錄因子 6 五、Cyclophilin 蛋白質 7 材料與方法 10 ㄧ、陰道滴蟲蟲株的處理 10 二、轉殖質體之建構 12 三、大腸桿菌雙雜交系統 19 四、GST-pull down 分析 21 五、蛋白質聚丙烯醯胺膠體電泳 24 六、西方轉漬 24 七、免疫螢光染色 25 八、酵素活性分析 25 九、CypA-1之反轉錄聚合酵素鏈反應 26 結果 27 ㄧ、大腸桿菌雙雜交實驗之篩選結果 27 二、CypA-1之保守性 27 三、CypA-1 之表現與分布位置 29 四、CypA-1及 R63A 之酵素活性 30 五、Myb1與 CypA-1作用專一性 31 六、Myb1與 CypA-1之相互作用 32 討論 35 ㄧ、大腸桿菌雙雜交實驗結果分析 35 二、Myb1與 CypA-1專一性作用之分析 36 三、Myb1與 CypA-1作用區域及作用方式之分析 36 四、CypA-1之保守性及酵素活性 38 附表 40 附圖 45 參考文獻 85 附錄 93 表 目 錄 表一、實驗使用之專一性引子 40 圖 目 錄 圖一、pBTmyb 誘餌載體 45 圖二、以大腸桿菌雙雜交實驗篩選與特定 Myb 作用之蛋白質 47 圖三、不同物種間 cyclophilin 之序列保守性 49 圖四、陰道滴蟲中 cyclophilin之序列保守性 51 圖五、CypA-1 之立體結構圖 53 圖六、不同物種間 FHA domain 之序列保守性 55 圖七、不同物種間 PP1-1 之序列保守性 57 圖八、CypA-1於陰道滴蟲中之分布與表現 59 圖九、CypA-1對 Myb1表現之影響 61 圖十、FHA 在陰道滴蟲中之分布與表現 63 圖十一、His-CypA-1及其突變株 His-R63A 蛋白質表現及純化 65 圖十二、CypA-1及其突變株 R63A 之酵素活性 67 圖十三、以大腸桿菌雙雜交實驗探討 CypA-1或 CypA-2與 Myb 之作用 69 圖十四、GST-CypA-1蛋白質表現及純化 71 圖十五、特定 Myb重組蛋白質與 CypA-1於試管內之相互作用 73 圖十六、以大腸桿菌雙雜交實驗探討 CypA-1內與 Myb1作用區域 75 圖十七、Myb1 上與 CypA-1專一作用區域之鑑定 77 圖十八、Myb1重組蛋白質與 CypA-1結合區域之分析 79 圖十九、HA-Myb1上 CypA-1作用區之功能 81 圖二十、HA-Myb1轉染株經 CsA 作用之結果 83 | |
| dc.language.iso | zh-TW | |
| dc.subject | 陰道滴蟲 | zh_TW |
| dc.subject | Myb1轉錄因子 | zh_TW |
| dc.subject | 脯氨酸異構脢 | zh_TW |
| dc.subject | 大腸桿菌雙雜交系統 | zh_TW |
| dc.subject | 酵素活性分析 | zh_TW |
| dc.subject | Peptidylprolyl cis-trans isomerase | en |
| dc.subject | Myb1 | en |
| dc.subject | Trichomonas vaginalis | en |
| dc.subject | bacterial two hybrid | en |
| dc.subject | Cyclophilin A | en |
| dc.title | 陰道滴蟲內 Myb1 與 Cyp A-1 蛋白質之交互作用 | zh_TW |
| dc.title | The interaction of the Myb1 and CypA-1 proteins in Trichomonas vaginalis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.advisor-orcid | ,戴榮湘(taijh@gate.sinica.edu.tw) | |
| dc.contributor.oralexamcommittee | 許翠瑛(Tsui-Ying Hsu) | |
| dc.subject.keyword | 陰道滴蟲,Myb1轉錄因子,脯氨酸異構脢,大腸桿菌雙雜交系統,酵素活性分析, | zh_TW |
| dc.subject.keyword | Trichomonas vaginalis,Myb1,Cyclophilin A,Peptidylprolyl cis-trans isomerase,bacterial two hybrid, | en |
| dc.relation.page | 109 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-19 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 微生物學研究所 | zh_TW |
| 顯示於系所單位： | 微生物學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 3.15 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。