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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃銓珍 | |
dc.contributor.author | Yuan-Kai Tu | en |
dc.contributor.author | 杜元凱 | zh_TW |
dc.date.accessioned | 2021-06-13T01:12:59Z | - |
dc.date.available | 2007-07-26 | |
dc.date.copyright | 2007-07-26 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-18 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29635 | - |
dc.description.abstract | 髓鞘鹼性蛋白(Myelin basic protein,MBP)是髓鞘的主要組成蛋白,髓鞘可緊密保裹神經纖維,也因為髓鞘的絕緣性質,使得神經傳導訊息可以進行有效率的傳送。許多組成髓鞘的蛋白質如MAG、OMGP、NOGO等,在許多研究已被指出具有抑制神經纖維生長的能力,因此本研究欲探討MBP是否也具有可使神經纖維外生長程度減弱的能力。首先,利用生物資訊方法與分子生物技術選殖出兩種不同形式的MBP(MBP1、MBP2),並以全胚胎原位雜合法探討其mRNA在斑馬魚胚胎的表現分佈情形,結果顯示MBP1、MBP2表現位置相同,但表現的時期則有差異。隨後構築MBP之表現質體,利用神經膠細胞專一性GFAP啟動子以表達MBP,以顯微注射技術將該MBP表現質體與可促進神經纖維外生長因子,如HBNF、NGF等共同注入斑馬魚單細胞魚卵,結果發現MBP具有導致神經纖維外生長減弱的現象。此外,為探討MBP減弱神經纖維生長的能力是否為EPO所影響,我們將MBP、HBNF和EPO共同注入斑馬魚的胚胎中,研究結果顯示,MBP減弱神經纖維生長的能力會受到 EPO 的影響。此外,為了探討MBP對於斑馬魚胚胎發育之影響,我們將針對 MBP 所設計的反義寡核苷酸(Morpholino oligonucleotide, MO)注射到斑馬魚胚胎中,結果發現,斑馬魚胚胎會出現生長遲緩、脊索發育不正常、魚體不規律顫抖等現象,表示MBP在斑馬魚發育過程中,在神經系統扮演相當重要的角色。最後,我們成功培育出MBP基因轉殖魚,並用以佐證前實驗中所得到MBP影響神經纖維生長的結果。 | zh_TW |
dc.description.abstract | Myelin basic protein(MBP) is the major constitutional component in vertebrate myelin, which wraps axon rigidly and forms multilayered compact myelin sheath. The insulation nature of myelin sheath makes neuronal conduction impulse more efficient and faster. Many proteins which make up myelin including MAG, OMGP and NOGO, have been shown to be able to inhibit neurite outgrowth. In this study, we cloned zebrafish MBP genes and investigate whether fish MBP has similar function or not. First, by using bioinformatics and PCR technology, we cloned two zebrafish MBP isoforms: MBP1 and MBP2, and then analyzed the temporal and spatial expression pattern of MBP1 and MBP2 during zebrafish development by whole-mount in situ hybridization. MBP1 and MBP2 had similar expression pattern, but MBP1 was expressed in earlier stage than MBP2. Furthermore, in order to investigate the roles of MBP1 and MBP2 in HBNF-induced neurite outgrowth in zebrafish embryos, we co-injected MBP1/MBP2 and HBNF into zebrafish embryos. It was found that the degree of neurite outgrowth was decreased, and this decrease effect could be rescued by co-injecting with a neuroprotective factor, EPO. However, the reduction level of neurite outgrowth caused by MBP1 and MBP2 are different. In addition, by virtue of MO knockdown technology, we found some zebrafish embryos manifested developmental delay and died the next day after injecting MBP1-MO, and some MBP1 MO-injected embryos showed abnormal shake behaviors. In the case of MBP2-MO knockdown, we found obvious defect in the notochord formation during embryonic development. Finally, we generated a transgenic zebrafish line expressing MBP-GFP, and we utilized these MBP transgenic fishes to confirm above results of microinjection. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T01:12:59Z (GMT). No. of bitstreams: 1 ntu-96-R94b46040-1.pdf: 2131310 bytes, checksum: 00875b13115938ecd3e90543c2cbd0f5 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 章節目錄
章節目錄 Ⅰ 圖表目錄 Ⅱ 中文摘要 Ⅲ 英文摘要 Ⅳ 英文縮寫 Ⅴ 壹、序言 一、 髓鞘鹼性蛋白(Myelin basic protein, MBP) 1 二、 髓鞘化 8 三、 GFAP啟動子之活性分佈與相關特性 11 四、 以斑馬魚作為功能性基因體分析之實驗動物的優點 12 五、 研究目的與策略 15 貳、材料與方法 一、 斑馬魚基因之選殖 17 二、 MBP與相關基因於斑馬魚系統之表現分析 21 參、結果 一、 斑馬魚髓鞘鹼性蛋白MBP1與MBP2基因之選殖及分析 28 二、 全胚胎原位雜交法分析斑馬魚MBP1以及MBP2基因的表現 29 三、 利用MO弱化(Knockdown)技術研究MBP1與MBP2基因在中樞 神經系統發育過程中的角色 30 四、 斑馬魚MBP1與MBP2影響HBNF或NGF引起之神經纖維外生 長的能力 30 五、 斑馬魚紅血球生成素可減緩MBP1與MBP2抑制神經纖維外生 長的能力.... 31 六、 利用基因轉殖斑馬魚探討MBP1與MBP2基因的功能 32 肆、討論 34 伍、參考文獻 43 圖表目錄 表一、各物種MBP所對應的Accession NO 52 圖一(A)、斑馬魚與各物種MBP胺基酸序列比對 53 圖一(B)、斑馬魚與Murine 21.5 kDa MBP外顯子區域比較 54 圖二、原位雜合分析斑馬魚MBP1與MBP2在發育期間位置與時間的表 現樣式背面觀 55 圖三、原位雜合分析斑馬魚MBP1與MBP2在發育期間位置與時間的表 現樣式側面觀 57 圖四、斑馬魚胚胎注射MBP1反義寡核苷酸 (Morpholino oligonucleotide) (MBP1-MO)後所誘發的形態改變 59 圖五、斑馬魚胚胎注射MBP2反義寡核苷酸 (Morpholino oligonucleotide) (MBP2-MO)後所誘發的形態改變 60 圖六、實驗中所使用之表現質體中啟動子與基因關係圖 61 圖七、GFAP-MBP1-GFP與HuC-GFP/CMV-HBNF和pcDNA3-EPO-HA 共同注射斑馬魚胚胎對神經纖維生長造成的影響 62 圖八、GFAP-MBP2-GFP與HuC-GFP/CMV-HBNF和pcDNA3-EPO-HA 共同注射斑馬魚胚胎對神經纖維生長造成的影響 63 圖九、MBP注射所引起的神經纖維抑制現象以及EPO減緩抑制神經纖維 現象統計圖 64 圖十、MBP transgenic fish螢光樣式表現圖 65 圖十一、HuC-GFP/CMV-HBNF和CMV-GFP/CMV-EPO-S-GFP共同注射 MBP轉殖魚胚胎對神經纖維生長造成的影響 66 | |
dc.language.iso | zh-TW | |
dc.title | 斑馬魚髓鞘鹼性蛋白之基因表現與功能研究 | zh_TW |
dc.title | Gene expression and Functional Identification of Zebrafish Myelin Basic Proteins | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李明亭,陳宏文 | |
dc.subject.keyword | 斑馬魚,髓鞘鹼性蛋白,神經纖維,神經膠細胞, | zh_TW |
dc.subject.keyword | Zebrafish,MBP,Neurite,Glia, | en |
dc.relation.page | 51 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-20 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
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