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標題: | 探討魚油補充對於延緩乳癌生成之關鍵時期與空間記憶學習之影響 Studies the critical period of fish oil supplementation on preventing breast cancer incidence and improving spatial learning memory performance. |
作者: | Pei-Hsuan Hsieh 謝佩璇 |
指導教授: | 蘇慧敏(Hui-Min Su) |
關鍵字: | 魚油,乳癌,空間記憶, fish oil,breast cancer,spatial learning memory, |
出版年 : | 2007 |
學位: | 碩士 |
摘要: | 探討魚油補充對於減緩乳癌生成關鍵時期及對空間記憶學習之影響
懷孕飲食中若攝取較多ω6多元不飽和脂肪酸將提高胎兒日後罹患乳癌機率﹔另外胚胎與泌乳時期,正是大腦快速發育,若此時缺乏ω3多元不飽和脂肪酸,將會影響空間記憶形成。本實驗目的主要探討在胚胎泌乳期因母體攝取富含ω6但缺乏ω3多元不飽和脂肪酸之葵花油飼料下,並在不同時期給魚油補充,是否能有效降低子代罹患乳癌之風險並減緩空間記憶退化。 利用懷孕第一天的Sprague-Dawley母鼠,餵食葵花油(sunflower oil)之自製飼料下,或在胚胎泌乳期、青春期與成年期分別管餵魚油42天。出生後第55天,給予7,12-Dimethylbenzanthracene (DMBA)誘發荷爾蒙依賴型乳癌,並收集親代母鼠懷孕泌乳時期與子代雌鼠乳癌生成前後尿液,以高效能液相層析儀分析其中雌激素及其代謝產物。動物195天大時以Morris water maze檢測空間記憶學習能力,223天大時犧牲。 懷孕泌乳時期餵食葵花油之飼料,會增加子代乳癌險,而在胚胎泌乳時期給予魚油補充確實能降低子代乳癌生成,然而青春期補充魚油雖無法有效降低但卻具有延緩乳癌生成之趨勢。補充魚油者,其在空間記憶學習之表現較未補充者佳。 近年國人飲食型態改變,過高的油脂攝取量,增加罹患許多疾病的風險如乳乳癌與神經退化性疾病,故婦女於懷孕或泌乳期間,若能進行魚油的補充,將能有效降低子代日後罹患乳癌與記憶損傷的機率。 The aim of this study is to evaluate the period of fish oil supplement could prevent the breast cancer incidence studied in DMBA (7,12-dimethylbenzanthracene) induced Sprague-Dawley rat. Maternal diet was given with linoleic acid-rich sunflower oil at stage of fetus to lactation without or with fish oil supplement at stage of fetus to lactation, at age of puberty, or at age of adult for 42 days. Spatial memory performance was examined by Morris water maze at age of 195 days. Animals were scarified at the age of 223 days old. It was found that maternal dietary sunflower oil intake did increase the % of cumulative breast cancer incidence in their female offspring. While, the breast cancer incidence was reduced 50% in their female offspring via maternal fish oil supplementation during pregnancy and lactation. The fish oil supplementation during puberty did delay but not inhibit the breast cancer promotion. It is interesting to know that fish oil may enhance tumor growing, since the tumor size was larger in animals with fish oil supplementation. The spatial memory performance was improved in the animals with fish oil supplementation. It was concluded that maternal fish oil supplementation during pregnancy and lactation may be the critical period for the breast cancer prevention on their female offspring, while, fish oil supplementation during puberty may delay the breast cancer happening. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29505 |
全文授權: | 有償授權 |
顯示於系所單位: | 生理學科所 |
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