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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 黃火鍊(Fore-Lien Huang) | |
| dc.contributor.author | Hsu-Ping Huang | en |
| dc.contributor.author | 黃旭平 | zh_TW |
| dc.date.accessioned | 2021-06-13T01:08:47Z | - |
| dc.date.available | 2007-07-30 | |
| dc.date.copyright | 2007-07-30 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-19 | |
| dc.identifier.citation | Autieri, M. V., and Carbone, C. J. (1999). 14-3-3Gamma interacts with and is phosphorylated by multiple protein kinase C isoforms in PDGF-stimulated human vascular smooth muscle cells. DNA Cell Biol 18, 555-564.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29503 | - |
| dc.description.abstract | 14-3-3蛋白家族在真核生物中具有高度保守性，為大小約30kDa的酸性蛋白質。目前研究已知有兩百種以上的磷酸化蛋白能與14-3-3家族蛋白結合。這些蛋白幾乎參與細胞內所有的活動，如細胞週期、訊號傳遞、細胞凋亡等。斑馬魚已知的14-3-3家族共有十一種，實驗室已成功選殖其中九種，論文中將探討斑馬魚14-3-3家族蛋白的生化特性，首先利用大腸桿菌系統大量表現並純化帶有GST的斑馬魚14-3-3家族融合蛋白和帶有六個His的斑馬魚14-3-3家族融合蛋白。將已純化帶有六個His的14-3-3-β2、ζ1、ζ2、ε1及η蛋白，注射至兔子體內，以產生多株抗體，並分析抗體特性。根據實驗室先前實驗結果，在斑馬魚胚胎時期，過量表現14-3-3-ζ1於心臟，可觀察到斑馬魚心臟出現心律不整或心跳減緩的現象。因此利用與斑馬魚相似性很高的鯉魚之心臟萃取物，與斑馬魚14-3-3-ζ1進行GST pull down，並使用液相層析與串聯質譜儀比對斑馬魚蛋白資料庫進行分析，辨認出共十四種蛋白，其中包含β-actin-1。進一步驗證β-actin-1與14-3-3-ζ1的結合作用，利用GST pull down實驗發現在試管中β-actin-1與14-3-3-ζ1確實有結合反應。而將β-actin-1與14-3-3-ζ1轉染至COS-1細胞中，進行共同免疫沉澱，兩蛋白的結合反應依然存在。證實了斑馬魚β-actin-1與14-3-3-ζ1在試管中與細胞中皆有交互作用。我們利用相同的方法，驗證九種14-3-3家族蛋白的成員與β-actin-1的交互作用，透過GST pull down可觀察到在試管中，九種14-3-3家族蛋白皆與β-actin-1產生結合作用；而在細胞中，僅能發現ζ1、ζ2以及η這三個14-3-3家族成員，與β-actin-1有較強的結合反應。 | zh_TW |
| dc.description.abstract | The 14-3-3 proteins form a family of highly conserved acidic proteins in all eukaryotic cells with a subunit mass of approximately 30 kDa. 14-3-3 proteins were capable of interacting with more than 200 different phosphorylated proteins. The binding partners are involved in almost every cellular process, like cell cycle control, apoptosis and signal transduction. The zebrafish 14-3-3 gene family consists of 11 distinct 14-3-3 genes. Previously, our laboratory had cloned 9 members of the zebrafish 14-3-3 gene family. In our study, we have expressed and purified GST fusion proteins and His-tagged fusion proteins of the 9 zebrafish 14-3-3 protein from E.coli. His-tagged 14-3-3 recombinant proteins were then injected into rabbit to obtain polyclonal antibodies against 14-3-3 β2, ζ1 ,ζ2 ,ε1 and η; these antibodies were also characterized. Proteins pull downed by GST fusion 14-3-3-ζ1 in the carp’s heart extracts were analyzed to identify potential interacting partners of 14-3-3-ζ1. 14 proteins, including β-actin-1, were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) of the 14-3-3-ζ1 GST pull downed proteins. The interaction between 14-3-3-ζ1 and β-actin-1 was further confirmed by GST pull down and co-immunoprecipitation in COS-1 cells. Our results indicate that β-actin-1 is a binding partner of 14-3-3-ζ1 both in vitro and COS-1 cells. Furthermore, the interaction between other 14-3-3 isoforms and β-actin-1 were also analyzed. We demonstrated that all 14-3-3 family proteins are able to bind β-actin-1in the GST pull down assay in vitro. However, only three 14-3-3 isoforms including ζ1, ζ2 and η have the ability to interact with β-actin-1 in COS-1 cells. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T01:08:47Z (GMT). No. of bitstreams: 1 ntu-96-R94b43021-1.pdf: 1755915 bytes, checksum: ea98245aad6c6c9cad6056990998bc2d (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 目錄
中文摘要…………………………………………………………...…....................... i 英文摘要 (Abstract)…………………………………………….…......................... ii 目錄…………………………………………………………...........…....................... iii 圖表目錄…………………………………………………………………………….. iv 壹、序言…………………………………………………………….......................... 1 一、14-3-3的基本介紹…………………………………………………………. 1 二、14-3-3的蛋白質結構………………………………………………………. 2 三、14-3-3與受質的結合………………………………………………………. 3 四、14-3-3的作用機制…………………………………………………………. 4 五、14-3-3與受質結合的調控……………………………………………….… 5 六、14-3-3自身的調控……………………………………………….…………. 9 貳、實驗目標………………….................................................................................. 11 参、實驗材料及方法……………………………………………………………….. 13 Ⅰ 實驗材料……….………………………………………………….....……… 13 Ⅱ 實驗方法…………………………………………………………………….. 13 一、表達及純化14-3-3家族融合蛋白……………………………………...… 14 二、利用西方點墨法方西14-3-3蛋白家族之抗體…………………………. 18 三、利用GST pull down研究14-3-3-zeta1與鯉魚的心臟萃取物的交互作用蛋白………………………………………………………………….….... 19 四、選殖斑馬魚β-actin-1基因……….……………………………….……… 20 五、GST pull down及共同免疫沉澱法驗證斑馬魚14-3-3家族蛋白與β-actin-1的結合.............................................................................................. 22 肆、實驗結果…………………………………………………….............................. 26 一、載體的構築……………………………………………..…………….……… 26 二、利用大腸桿菌系統表現及純化斑馬魚14-3-3 家族GST重組蛋白……….. 26 三、利用大腸桿菌系統表現帶有六個His的斑馬魚14-3-3 家族重組蛋白…. 27 四、以西方點墨法分析斑馬魚14-3-3多株抗體的特性………………………. 27 五、以GST pull down篩選鯉魚心臟的萃取物中與斑馬魚14-3-3-zeta1的GST 融合蛋白有交互作用的蛋白質並利用液相層析與串聯質譜儀分析…………………………………………………………………………….. 28 六、利用GST pull down分析斑馬魚14-3-3-zeta1與β-actin-1的交互作用.… 28 七、利用共同免疫沉澱技術分析斑馬魚14-3-3-zeta1與β-actin-1在COS-1細胞中的交互作用………………………………………………………….. 29 伍、討論…………………………………………………………………………….. 31 陸、參考文獻……………………………………………………………………….. 36 圖表目錄 表一、利用西方點墨法分析斑馬魚14-3-3多株抗體的特性…………………. 46 表二、液相層析與串聯質譜儀比對斑馬魚蛋白質資料庫分析結果…………. 47 圖一、針對實驗所構築之載體……………………………………………........ 48 圖二、純化大腸桿菌系統表現之斑馬魚14-3-3家族 GST重組蛋白……….. 49 圖三、純化大腸桿菌系統表現之帶有六個His的斑馬魚14-3-3 family重組蛋白…………………………………………………………….………... 51 圖四、以西方點墨法分析斑馬魚14-3-3多株抗體的特性............................... 52 圖五、篩選鯉魚心臟的萃取物中與斑馬魚14-3-3-zeta1有交互作用的蛋白質................................................................................................................ 53 圖六、斑馬魚14-3-3在in vitro與β-actin-1的交互作用………….………… 54 圖七、斑馬魚14-3-3與β-actin-1在COS-1細胞中的交互作用…………….. 55 圖八、將斑馬魚14-3-3家族轉染至COS-1細胞….......................................... 57 圖九、分析人類14-3-3-zeta與本實驗室選殖的九種14-3-3家族蛋白質序列………………………………………………………………………… 58 圖十、比對斑馬魚14-3-3-zeta1、zeta2之蛋白序列差異………………......... 59 附錄一、14-3-3蛋白質結構……………………………………………............ 60 附錄二、14-3-3蛋白的作用機制………………………………………............. 61 附錄三、選殖九種斑馬魚14-3-3蛋白家族……………………………………. 62 附錄四、於斑馬魚胚胎心臟過量表現14-3-3造成心跳減緩………………… 63 附錄五、14-3-3家族演化樹…………………………………………….............. 64 附錄六、斑馬魚14-3-3家族共十一種家族成員的基因結構…………............ 65 | |
| dc.language.iso | zh-TW | |
| dc.title | 斑馬魚14-3-3蛋白家族的純化與性質鑑定 | zh_TW |
| dc.title | Expression, purification and characterization of 14-3-3 proteins from zebrafish | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃銓珍,李明亭 | |
| dc.subject.keyword | 14-3-3, | zh_TW |
| dc.relation.page | 45 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-23 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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