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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林中天 | |
dc.contributor.author | Chia-Yun Hsieh | en |
dc.contributor.author | 謝佳紜 | zh_TW |
dc.date.accessioned | 2021-06-13T01:04:39Z | - |
dc.date.available | 2007-07-27 | |
dc.date.copyright | 2007-07-27 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-24 | |
dc.identifier.citation | References
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/29328 | - |
dc.description.abstract | 實驗目的: 比較纖維蛋白組織膠(fibrin glue)與兩種商品化之氰基丙烯酸酯衍生物(cyanoacrylate; CA derivatives),其對於角膜毒性、抗菌性及黏合角膜切口之能力。
實驗方法: 使用三種組織膠,分別為fibrin glue、N-butyl cyanoacrylate(N-butyl CA)與methoxypropyl cyanoacrylate(methoxypropyl CA)。於體外培養之牛類角膜上皮、內皮與基質細胞分別點上組織膠來觀察其對於角膜細胞之毒性。抗菌能力則是使用眼科常見感染之細菌來測試,分別為:Staphylococcus aureus、Streptococcus pneumoniae、Pseudomonas aeruginosa、Escherichia coli 與 Mycobacterium chelonae。於剛劃好菌之培養基點上組織膠,觀察其對於細菌之影響,並測量抑菌圈寬度做為比較。黏合角膜切口之能力,以角膜切割刀於兔眼角膜上做一切口,並且點上組織膠,之後逐漸增加兔眼之眼內壓,觀察組織膠最大可承受之眼內壓。 實驗結果: Methoxypropyl CA對於角膜之毒性為最強,纖維蛋白組織膠則是完全沒有角膜毒性(p<0.01)。Methoxypropyl CA與N-butyl CA對於Staphylococcus aureus、Streptococcus pneumoniae與 Mycobacterium chelonae有抑菌效果,但是對於Pseudomonas aeruginosa、Escherichia coli則是沒有任何影響。Fibrin glue則亦無任何抑菌效果(p<0.01)。在黏合角膜切口能力方面,methoxypropyl CA的效果最好,其次為N-butyl CA,至於fibrin glue的效果最弱,但結果也與對照組相近。 結論:對於角膜毒性、抑菌與黏合角膜切口之能力,三種組織膠都顯現不同之特性。在角膜手術選擇組織膠時,都需要考量這些不同之性質。 | zh_TW |
dc.description.abstract | Purpose: To compare the corneal cytotoxicity, bacteriostatic effect, duration of adherence and the ability to seal corneal incisions among fibrin glue and two commercially available cyanoacrylate derivatives.
Methods: Fibrin glue, N-butyl cyanoacrylate and methoxypropyl cyanoacrylate were compared. Corneal cytotoxicity was tested by direct contact method using bovine corneal epithelial cells, keratocytes and corneal endothelial cells challenged with droplets of adhesives. The bacteriostatic activity was verified by measuring the inhibition zones of bacterial growth surrounding adhesive droplets on agar plates inoculated with Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Mycobacterium chelonae. The duration of adherence was evaluated based on the morphological changes of adhesive drops when applied on cell culture system. The ability to seal corneal incisions was verified by the maximum resisted intraocular pressures before leakage of the rabbit corneal stab wounds sealed with tissue adhesives. Results: Methoxypropyl cyanoacrylate demonstrated the greatest corneal cytotoxocity followed by N-butyl cyanoacrylate. Fibrin glue showed no detectable cytotoxicity (p<0.01). Methoxypropyl cyanoacrylate and N-butyl cyanoacrylate showed bacteriostatic effects against Staphylococcus aureus, Streptococcus pneumoniae, and Mycobacterium chelonae but not Pseudomonas aeruginosa and Escherichia coli, while fibrin glue had no such effect against both Gram-positive and negative bacteria (p<0.01). Methoxypropyl cyanoacrylate and N-butyl cyanoacrylate demonstrated longer duration of adherence and greater ability to seal corneal incisions than fibrin glue (p<0.01). Conclusions: There is difference of corneal cytotoxicity, bacteriostatic effect, duration of adherence and the ability to seal corneal incisions among fibrin glue, N-butyl cyanoacrylate and methoxypropyl cyanoacrylate. These different properties should be considered when choosing tissue adhesives for corneal surgeries. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T01:04:39Z (GMT). No. of bitstreams: 1 ntu-96-R93629029-1.pdf: 1883161 bytes, checksum: 7c6c6ccb1470da6b53e13321031a033a (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 目錄
口試委員會審定書..........................................i 誌謝.....................................................ii 中文摘要................................................iii 英文摘要..................................................v 第一章 緒言.............................................1 第一節 角膜潰瘍之治療...................................1 第二節 組織膠於眼睛之應用...............................1 第三節 研究目的.........................................2 第二章 文獻探討.........................................3 第一節 人與犬貓角膜潰瘍、穿孔之治療.....................3 一、 人角膜潰瘍、穿孔之治療...........................3 二、 犬貓角膜潰瘍、穿孔之治療.........................6 第二節 組織膠之介紹.....................................7 一、 氰基丙烯酸酯.....................................7 二、 纖維蛋白組織膠..................................10 三、 其他研發中之組織膠..............................14 第三節 組織膠於眼睛疾病之應用..........................15 一、 組織膠於人類眼睛之應用..........................15 二、 組織膠於犬貓眼睛之應用..........................24 第三章 實驗材料與方法 ..............................26 第一節 組織膠..........................................26 第二節 細胞培養........................................28 一、 牛角膜上皮細胞之初代培養........................28 二、 牛角膜基質細胞之初代培養........................29 三、 牛角膜內皮細胞之初代培養........................29 第三節 評估組織膠對於角膜細胞之毒性....................31 一、 直接細胞接觸法..................................31 二、 TUNEL染色法.....................................31 第四節 組織膠於細胞培養之環境的存留時間................33 第五節 以抑菌圈評估組織膠之抑菌能力....................33 第六節 測量組織膠黏合角膜切口之能力....................34 一、 使用連通裝置與Tono-pen XL測試不同液面時之眼壓...34 二、 測試組織膠黏合角膜切口之能力....................34 第七節 統計與分析方法..................................35 第四章 實驗結果........................................36 第一節 組織膠對於角膜細胞之毒性........................36 一、 直接細胞接觸法..................................36 二、 TUNEL染色法結果.................................37 第二節 組織膠於細胞培養之環境的存留時間................38 第三節 組織膠對於細菌之抑菌能力........................38 第四節 組織膠對於角膜切口之黏合能力....................38 第五章 討論............................................40 第一節 組織膠對於角膜細胞之毒性測試模式之選擇..........40 第二節 組織膠的存留時間比較............................42 第三節 組織膠對於細菌之抑菌能力與文獻之比較............43 第四節 測得眼壓實驗結果與文獻之討論....................47 第六章 結論............................................51 參考文獻.................................................52 圖目錄...................................................62 表目錄...................................................76 | |
dc.language.iso | zh-TW | |
dc.title | 比較纖維蛋白組織膠、N-butyl與methoxypropyl氰基丙烯酸酯之角膜毒性,抗菌效果以及黏合角膜切口之能力 | zh_TW |
dc.title | Fibrin Glue Versus N-butyl and Methoxypropyl Cyanoacrylate in Corneal Cytotoxicity, Bacteriostatic Effects and the Ability to Seal Corneal Incisions | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 陳偉勵 | |
dc.contributor.oralexamcommittee | 劉振軒,葉力森 | |
dc.subject.keyword | 氰基丙烯酸酯,纖維蛋白組織膠,角膜細胞,細胞毒性,抑菌V黏合能力, | zh_TW |
dc.subject.keyword | cyanoacrylate,fibrin glue,corneal cells,cytotoxicity,bacteriostatic effect,seal corneal incisions, | en |
dc.relation.page | 83 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-24 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
顯示於系所單位: | 獸醫學系 |
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