結合脂肪前驅細胞和膠原蛋白/玻尿酸凝膠之脂肪組織工程

Abstract

外傷、腫瘤切除或先天性畸形而造成的軟組織缺陷主要是起因於皮下脂肪組織的喪失。由於目前仍然沒有最為理想的植入物，所以皮下脂肪組織的修復仍是一個問題。而組織工程構造物是可以發展的選擇之ㄧ。本研究我們著重在脂肪前驅細胞結合可注射式的膠原蛋白/玻尿酸膠體在軟組織缺陷重建上的應用。我們將分離自Wistar公鼠脂肪組織的脂肪前驅細胞培養在添加不同比例玻尿酸的膠原蛋白中，來進行活體外、活體內的實驗。其中特別的是，我們使用磁振造影(MRI)來觀察皮下注射的細胞膠體構造物。研究結果我們發現將玻尿酸添加在膠原蛋白膠體中能促進脂肪前驅細胞的增生，也能抑制膠體的收縮。而且在組織學分析中，我們也觀察到皮下注射的細胞膠體中有成熟的脂肪細胞生成。研究結果證實了脂肪前驅細胞結合膠原蛋白/玻尿酸膠體做為活體內軟組織生成的材料是相當具有潛力的。

Soft tissue defects following trauma, tumor resections, or congenital deformities are mostly caused by the loss of subcutaneous adipose tissue. The restoration of subcutaneous adipose tissue remains an unresolved problem since satisfactory implant materials are still not available. Engineered tissues are being developed as alternatives. We here focus on the application of preadipocytes and injectable collagen/ hyaluronic acid gels for soft-tissue defect reconstruction. Preadipocytes isolated from the adipose tissue of male Wistar rats were cultured in collagen gels with various ratios of hyaluronic acid for in vitro and in vivo experiments. Particularly, the cell-gel constructs were evaluated with magnetic resonance imaging (MRI) after subcutaneous injection. We found the addition of hyaluronic acid in collagen gels increased proliferation of the preadipocytes, and limited contraction of the gels. Mature adipocytes were observed in the cell-gel constructs after subcutaneous injection by histological analysis. These results support the promise of preadipocytes combined with collagen/ hyaluronic acid gels as a material for use in producing soft tissues in vivo.