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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 柯淳涵(Chun-Han Ko) | |
dc.contributor.author | Dong-Lin Sie | en |
dc.contributor.author | 謝東霖 | zh_TW |
dc.date.accessioned | 2021-06-13T00:32:22Z | - |
dc.date.available | 2012-07-30 | |
dc.date.copyright | 2007-07-30 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-26 | |
dc.identifier.citation | Bajpai, P. (1999) Application of Enzymes in the Pulp and Paper Industry. Biotechnology Progress. 15(2): 147-157.
Beg, Q. K., M. Kapoor, L. Mahajan, and G. S. Hoondal (2001) Microbial xylanases and their industrial applications: a review. Applied Microbiology and Biotechnology. 56: 326-338. Biely, P. (1985) Microbial xylanolytic system. Trends in Biotechnology 3: 286-301. Brown, J., M. C. Cheek, H. Jameel and T. W. Joyce (1994) Medium-consistency ozone bleaching with enzyme pretreatment. Tappi Journal. 77(11): 105-109. Buchert, J., E. Bergnor, G. Lindblad, L. Viikari and M. Ek (1997) Significance of xylan and glucomannan in the brightness reversion of kraft pulps. Tappi Journal. 80(6): 165-171. Chauvet, J. M., J. Comtat and P. Noe (1987) Proceedings of the 4th Intl. Symposium on Wood and Pulping Chemistry. Vol 2. EuCePa, Paris. p. 325. Daneault, C., C. Leduc and J. L. Valade (1994) The use of xylanases in kraft pulp bleaching: a review. Tappi Journal. 77(6):125-131. de Jong, E., K. K. Y. Wong and J. N. Saddler (1997) The mechanism of xylanase prebleaching of kraft pulp: An examination using model pulps prepared by depositing lignin and xylan on cellulos fibers. Holzforschung. 51:19-26. Iversen, T. and S. Wannstrom (1986) Lignin-carbohydrate bonds in a residual lignin isolated from pine kraft pulp. Holzforschung. 40(1): 19-22. Jurasek, L. and M. Paice (1992) Proceedings of the International Symposium on Pollution Prevention in the Manufacture of Pulp & Paper-Opportunities and Barriers.Washington, DC. Kantelinen, A., M. Ratto, J. Sundquist, M. Ranua, L. Viikari and M. Linko (1988) Proceedings of the Tappi International Pulp Bleaching Conference. Orlando, FL, June 5-9. p. 1-9. Kantelinen, A., B. Hortling, J. Sundquist, M. Linko and L. Viikari (1993) Proposed mechanism of the enzymatic bleaching of kraft pulp with xylanases. Holzforschung. 47(4): 318-324. Karlsson, O. and U. Westermark (1996) Evidence for chemical bonds between lignin and cellulose in kraft pulps. Journal of Pulp and Paper Science. 22(10): J397-J401. Kerr, A. J. and D. A. I. Goring (1975) Role of hemicellulose in delignification of wood. Canadian Journal of Chemistry-Revue Canadienne de Chimie. 53(6): 952-959. König, J., R. Grasser, H. Pikor and K. Vogel (2002) Determination of xylanase, β-glucanase, and cellulase activity. Analytical and bioanalytical chemistry 374:80-87. Kulkarni, N., A. Shendue, and M. Rao. (1999) Molecular and biotechnological aspects of xylanases. FEMS Microbiology Reviews. 23: 411-456. Miller, G. L (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31: 426-428. Paice, M. G. and L. Jurasek (1984) Removing hemicellulose from pulps by specific enzymic-hydrolysis. Journal of Wood Chemistry and Technology. 4(2): 187-198. Procter, A. R. (1972) Kraft delignification rate and hemicellulose content. Tappi Journal. 55(3): 424. Senior, D. J. and J. Hamilton (1992a) Use of xylanases to decrease the formation of AOX in kraft pulp bleaching. Journal of Pulp and Paper Science. 18(5): 165-169. Senior, D. J. and J. Hamilton (1992b) Biobleaching with xylanases brings biotechnology to reality. Pulp & Paper. 66(9): 111. Senior, D. J. and J. Hamilton (1993) Xylanase treatment for the bleaching of softwood kraft pulps: the effect of chlorine dioxide substitution. Tappi Journal. 76(8): 200-206. Senior, D. J., J. Hamilton, R. L. Bernier and J. R. du Manoir (1992) Reduction in chlorine use during bleaching of kraft pulp following xylanase treatment. Tappi Journal. 75(11): 125-130. Shah, A. K., A. Cooper, R. B. Adolphson, X.-L. LI, H. Chen, L. G. Ljungdahl and K.-E. L. Eriksson (2000) Use of an extremely high specific activity xylanase in ECF and TCF pulp bleaching. Tappi Journal Peer Reviewed Paper. p. 1-12. Skerker, P. S., N. Beerman and M. M. Labbauf (1992) TAPPI 1992 Pulping Conference Proceedings. Book 1. Tappi Press. Atlanta. p. 27. Teleman, A., V. Harjunpää, M. Tenkanen, J. Buchert, T. Hausalo, T. Drakenberg and T. Vuorinen (1995) Characterization of 4-deoxy-β-L-threo-hex-4- enopyranosyluronic acid attached to xylan in pine kraft pulp and pulping liquor by H1 and C13 NMR-spectroscopy. Carbohydrate Research. 272(1): 55-71. Tolan, J. and R. V. Canovas (1992) The use of enzymes to decrease the CL2 requirements in pulp bleaching. Pulp Paper-Canada. 93(5): 39-42. Tolan, J. S. (1992) TAPPI 1992 Pulping Conference Proceedings. Book 1. p. 13. Viikari, L., M. Ranua; A. Kantelinen, J. Sundquist and M. Linko (1986) Proceedings of the 3rd Internatinal Conference on Biotechnology in the Pulp and Paper Industry. Stockholm, Sweden, June 16-19. p. 67. Viikari, L., M. Ranua, A. Kantelinen, M. Linko and J. Sundquist (1987) Proceedings of the 4th International Symposium on Wood & Pulping Chemistry. Paris, April 27-30. 1: 151. Yamasaki, T., S. Hosoya, C. -L. Chen, J. S. Gratzl and H. M. Chang (1981) Characterization of residual lignin in kraft pulp. Proceedings of the Intl. Symposium on Wood and Pulping Chemistry. Vol. II, p. 34. Yang, J. L., G. Lou and K. E. L.Eriksson (1992) The impact of xylanase on bleaching of kraft pulps. Tappi Journal. 75(12): 95-101. Ziobro, G. C. (1990) Origin and nature of kraft color.1. Role of aromatics. Journal of Wood Chemistry and Technology. 10(2): 133-149. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28968 | - |
dc.description.abstract | Paenibacillus sp. isolate 2S-3 與2S-6是由製漿漂前黑液中所分離出的菌種。本研究的黑液是由中華紙漿公司收集得來,2S-3以及2S-6皆具有木聚醣酶活性,並利用16S rDNA定序鑑定出與其他菌種的親緣關係。
分析結果顯示2S-3產生的木聚醣酶大小為40kDa,2S-6會因SDS與sample buffer而變性,所以利用膠體層析分析2S-6產生的木聚醣酶大小為37.65kDa。菌株2S-3、2S-6所產生的粗酵素液最適合的溫度與pH為pH 7、溫度分別在70oC、60oC,利用碳源木聚醣(birchwood xylan)、米糠(rice bran)、米殼(rice husk)與氮源0.5%酵母抽出物(yeast extract)加0.5%蛋白棟(peptone)、大豆粉(soy bean)可誘導產生木聚醣酶,2S-3與2S-6分別以基質2%木聚醣、0.5%酵母抽出物、0.5%蛋白棟培養會有最高活性6.1 IU/ml和5.6 IU/ml。 將硫酸鹽尤加利紙漿以粗木聚醣酶酵素液每克絕乾漿5 IU作前處理後,再進行C70/D30-Eo-D漂白,菌株2S-3和2S-6所分泌之木聚醣酶前處理漂白後所測得的白度90.1,相較於對照組88.2皆有明顯的提升,由結果發現木聚醣酶前處理能節省漂白藥劑、增進漂白效果,足證此等木聚醣酶具有進一步研究應用的潛力。 | zh_TW |
dc.description.abstract | Paenibacillus sp. isolate 2S-3 and 2S-6 were isolated by screening bacterial strains from the thermoalkaline black liquor. The black liquor was collected from Chung Hwa Pulp Corporation. Strains 2S-3 and 2S-6 exhibited xylanase activities and its’ phylogenetic relationship with other bacteria were identified by 16S rDNA sequencing. The xylanase of strain 2S-3 and 2S-6 have been analyzed.
The xylanase produced from 2S-3 was analyzed in zymogram and showed a molecular mass of about 40 kDa. 2S-6 xylanase could not analyze in zymogram because xylanase would denature with SDS and sample buffer. Using gel chromatography column to determine molecular mass of crude xylanase was the suitable way and showed the molecular mass of about 37.65 kDa. The optimum temperature and pH for crude enzyme of 2S-3 and 2S-6 were 70oC and pH 7, 60oC and pH 7. The results showed Paenibacillus sp. isolate 2S-3 and 2S-6 used different carbon and nitrogen sources to induce xylanase. The carbon source included 2% xylan, rice bran, rice husk and nitrogen source contained 0.5% yeast extract plus 0.5% peptone, soy bean, corn steep liquor (C .S .L.), (NH4)2SO4. The highest xylanase activities of 2S-3 and 2S-6 were 6.1 IU/ml and 5.6 IU/ml with medium contained 2% xylan, 0.5% yeast extract and 0.5% peptone. After pretreated by 2S-3 and 2S-6 xylanases at 5 IU per gram oven dry pulp (o. d. p), eucalypt kraft pulp was bleached by C70/D30-Eo-D sequence. In 2S-3 and 2S-6, 90.1% brightness were achieved by both xylanase pretreatment, compared by 88.2% from controls. Result showed xylanase pretreatment can be integrated into different bleaching sequences. It can save bleaching chemical usages and enhance brightness of final products. The results validated the efficiency of xylanase and warranted studies for further application. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T00:32:22Z (GMT). No. of bitstreams: 1 ntu-96-R94625029-1.pdf: 938231 bytes, checksum: c39684b1b89bdbcba9e17023ed03d986 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | Index
Page 摘要................................................................................................. i Abstract........................................................................................... ii Index................................................................................................ iv Table index...................................................................................... vii Figure index..................................................................................... v Ⅰ Introduction...................................................................................... 1 Ⅱ Literature reviews............................................................................ 5 1. Characterization and structure of xylan.................................. 5 2. Xylanolytic enzymes................................................................... 6 3. Developing of bleaching technology.......................................... 7 4. Prebleaching with xylanase………………………………….……… 9 4.1 Origin of xylanase in bleaching........................................................ 9 4.2 Lignin-xylan complexes...................................................... 10 4.3 Physical entrapment of lignin.................................................. 11 4.4 Factors affecting treatment efficiency..................................... 11 4.5 Effect on chemical consumption............................................. 12 4.6 Effect on pulp properties......................................................... 13 4.7 Effect on bleaching Effluents.................................................. 14 Ⅲ Materials and methods.................................................................. 16 1. Screening, isolation and identification of bacterial strains.. 17 2. 16S rDNA and phylogenetic tree............................................ 17 3. Polysaccharide hydrolase assay.............................................. 18 3.1 Stain assay of polysaccharide hydrolases (Congo red stain).. 18 3.2 SDS-PAGE and zymogram.................................................... 18 3.3 DNSA assay............................................................................ 19 4. Characterization of strain 2S-3, 2S-6..................................... 22 4.1 Gram stain............................................................................... 22 4.2 Catalase, Oxidase, Indole and H2S test................................... 23 5. Growth curve and crude xylanase of strains 2S-3, 2S-6....... 24 6. Effect of carbon and nitrogen sources on xylanase activity.... 24 7. Pulp properties and conditions of bleaching......................... 25 8. Analytical methods.................................................................. 26 Ⅳ Results and discussions................................................................ 27 1. Screening, isolation and identification of bacterial strains....... 27 2. Polysaccharide hydrolase assay (Congo red stain assay of polysaccharide hydrolases)...................................................... 27 3. Gram stain of strain 2S-3 and 2S-6.......................................... 30 4. Physiological and biochemical characteristics (Catalase, Oxidase, Indole and H2S test)…………...……………………………. 30 5. 16S rDNA and phylogenetic tree……………………..….… 32 6. SDS-PAGE and zymogram of 2S-3 xylanase……….….….. 35 7. Xylanase assay…………………………………………...… 38 7.1 Effect of culture conditions on growth and crude xylanase of strains 2S-3, 2S-6…………………………………......…. 38 7.2 Effect of carbon and nitrogen sources on xylanase activity. 41 8. Pulp properties and conditions of bleaching…………….…. 43 Ⅴ Conclusion……………………………………………………….………… 46 Ⅵ References...................................................................................... 48 Table Index Page Table 1 Conditions in pretreatment and bleaching stages…………………….… 25 Table 2 Physiological and biochemical characteristics of Peanibacillus sp. 2S-3 and 2S-6………………………………………………….………..…... 31 Table 3 Chemical properties of oxygen-delignified pulp treated with 2S-3 xylanase prior to (C70/D30)EoD sequence………………………….. 44 Table 4 Chemical properties of oxygen-delignified pulp treated with 2S-6 xylanase prior to (C70/D30)EoD sequence…………………………… 44 Table 5 Chemical properties of oxygen-delignified pulp treated with xylanase (2S-3, 2S-6, and pulpzyme HC) prior to (C70/D30)EoD sequence.….. 45 Figure Index Page Figure 1 Chemical structure of xylan……………………..…………………....… 6 Figure 2 A hypothetical plant xylan structure showing different substituent groups with sites of attack by microbial xylanase…………………...……...…. 7 Figure 3 Flow chart of experimental……………………….……….………...…. 16 Figure 4 Polysaccharide hydrolases of strain 2S-3……………………………… 28 Figure 5 Polysaccharide hydrolases of strain 2S-6…………………………….... 29 Figure 6 Gram stain of Paenibacillus sp. 2S-3 and 2S-6………………………... 30 Figure 7 Phylogenetic relationship of 2S-3 to Paenibacillus based on 16S rDNA analysis…………………..………………………………..….......…… 33 Figure 8 Phylogenetic relationship of 2S-6 to Paenibacillus based on 16S rDNA analysis……………………………………………...….…….…….…. 34 Figure 9 SDS-PAGE and zymography of 2S-3 xylanase………………….…….. 35 Figure 10 Chromatography of protein (standard sample) and crude xylanase….. 37 Figure 11 Calibration curve of protein…………………………….…….….….. 38 Figure 12 Growth curve of strain 2S-3 and 2S-6 are separated at 30oC and 50oC... 39 Figure 13 Xylanase of strain 2S-3 and 2S-6 are produced in two medium……... 39 Figure 14 Crude xylanase activity of 2S-3 and 2S-6 are incubated at 37oC…...... 40 Figure 15 Crude xylanase of strain 2S-3 and 2S-6 are incubated at pH 7…….… 40 Figure 16 Effect of strain 2S-3 and 2S-6 which were cultivated by immobile carbon source (rice husk) and four nitrogen sources on xylanase activity….... 42 Figure 17 Effect of strain 2S-3 and 2S-6 which were cultivated by immobile carbon source (rice bran) and four nitrogen sources on xylanase activity…..... 42 Figure 18 Effect of strain 2S-3 and 2S-6 which were cultivated by immobile carbon source (xylan) and four nitrogen sources on xylanase activity……….. 43 | |
dc.language.iso | en | |
dc.title | Paenibacillus sp. Isolate 2S-3與2S-6菌種特性分析與紙漿漂白前處理應用 | zh_TW |
dc.title | Characteristics of Paenibacillus sp. Isolate 2S-3, 2S-6 and Application of Its Xylanase Pretreatment for Kraft Pulp Bleaching | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 劉佳振,張上鎮(Shang-Tzen Chang),杜鎮(Jenn Tu),蕭英倫(Ing-Lun Shiau) | |
dc.subject.keyword | 木聚醣酶,漂白,尤加利樹,硫酸鹽漿,Paenibacillus, | zh_TW |
dc.subject.keyword | Xylanase,Bleaching,Eucalypt,kraft pulp,Paenibacillus, | en |
dc.relation.page | 62 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-26 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 森林環境暨資源學研究所 | zh_TW |
顯示於系所單位: | 森林環境暨資源學系 |
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