請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28902
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林璧鳳 | |
dc.contributor.author | Ming-Chih Hsu | en |
dc.contributor.author | 許銘志 | zh_TW |
dc.date.accessioned | 2021-06-13T00:28:42Z | - |
dc.date.available | 2009-07-27 | |
dc.date.copyright | 2007-07-27 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-26 | |
dc.identifier.citation | 李佩芸 ( 2005 ) 口服 Dp2 重組蛋白質對致敏小鼠過敏免疫調節之影響。台灣大學微生物與生化學研究所碩士論文。
謝貴雄 ( 1995 ) 小兒氣喘病之預防和處理。中華民國兒科醫學會雜誌,36 : S9-20。 National Institute of Health. National Heart, Lung, and Blood Institute. ( 1997 ) Guideline for the diagnosis and management of asthma. No.97-4051. Akbar, A. N., Vukmanovic-Stejic, M., Taams, L. S. and Macallan, D. C. ( 2007 ) The dynamic co-evolution of memory and regulatory CD4+ T cells in the periphery. Nat. Rev. Immunol. 7 : 231-237. Akdis, C. A., Blaser, K. and Akdis, M. ( 2006 ) Mechanism of allergen-specific immunotherapy. Chem. Immunol. Allergy. 91 : 195-203. Allan, S. E., Passerini, L., Bacchetta, R., Crellin, N., Dai, M., Orban, P. C., Ziegler, S. F., Roncarolo, M. G. and Levings, M. K. ( 2005 ) The role of 2 FOXP3 isoforms in the generation of human CD4+ Tregs. J. Clin. Invest. 115 : 3276-3284. Aramaki, Y., Fujii, Y., Suda, H., Suzuki, I., Yadomae, T. and Tsuchiya, S. ( 1994 ) Induction of oral tolerance after feeding of ragweed pollen extract in mice. Immunol. Lett. 40 : 21-25. Bach, I. ( 2000 ) The LIM domain: regulation by association. Mechanism of Development 91 : 5-17. Bachelet, I., Munitz, A., Mankutad, D. and Levi-Schaffer, F. ( 2006 ) Mast cell costimulation by CD226/CD112 (DNAM-1/Nectin-2): a novel interface in the allergic process. J. Bio. Chem. 281 : 27190-27196. Beasley, R. ( 2002 ) The burden of asthma with specific reference to the United States. J. Allergy Clin. Immunol. 109 ( 5 Suppl ) : S482-9. Berer, A., Stockl, J., Majdic, O., Wagner, T., Kollars, M., Lechner, K., Geissler, K. and Oehler, L. ( 2000 ) 1,25-Dihydroxyvitamin D(3) inhibits dendritic cell differentiation and maturation in vitro. Exp. Hematol. 28 : 575-583. Bettelli, E., Dastrange, M. and Oukka, M. ( 2005 ) Foxp3 interacts with nuclear factor of activated T cells and NF-kappa B to repress cytokine gene expression and effector functions of T helper cells. Proc. Natl. Acad. Sci. USA 102 : 5138-5143. Birkenmeier, E. H. and Gordon, J. I. ( 1986 ) Developmental regulation of a gene that encodes a cysteine-rich intestinal protein and maps near the murine immunoglobulin heavy chain locus. Proc. Natl. Acad. Sci. USA 83 : 2516-2520. Black, P. N. ( 2005 ) Does atopy protect against enteric infections? Allergy 60 : 30-34. Blank, M., George, J., Barak, V., Tincani, A., Koike, T. and Shoenfeld, Y. ( 1998 ) Oral tolerance to low dose beta 2-glycoprotein I: Immunomodulation of experimental antiphospholipid syndrome. J. Immunol. 161 : 5303-5312. Brownell, J. and Casale, T. B. ( 2004 ) Anti-IgE therapy. Immunol. Allergy Clin. North Am. 24 : 551-568. Brunkow, M. E., Jeffery, E. W., Hjerrild, K. A., Paeper, B., Clark, L. B., Yasayko, S. A., Wilkinson, J. E., Galas, D., Ziegler, S. F. and Ramsdell, F. ( 2001 ) Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat. Genet. 27 : 68-73. Brusasco, V. and Crimi, E. ( 2001 ) Methacholine provocation test for diagnosis of allergic respiratory diseases. Allergy 56 : 1114-1120. Carson, B. D., Lopes, J. E., Soper, D. M. and Ziegler, S. F. ( 2006 ) Insights into transcriptional regulation by FOXP3. Front. Biosci. 11 : 1607-1619. Chang, Y. C. and Hsieh, K. H. ( 1989 ) The study of house dust mite in Taiwan. Ann. Allergy 62 : 101-106. Chen, L., Xie, X., Zhang, X., Jia, W., Jian, J., Song, C. and Jin, B. ( 2003 ) The expression, regulation and adhesion function of a novel CD molecule, CD226, on human endothelial cells. Life Science 73 : 2373-2382. Chen, T. Q. and Shi, H. Z. ( 2006 ) CD28/CLAT-4-CD80/CD86 and ICOS-B7RP-1 costimulatory pathway in bronchial asthma. Allergy 61 : 15-26. Chen, Y., Inobe, J., Marks, R., Gonella, P., Kuchroo, V. K. and Weiner, H. L. ( 1995 ) Peripheral deletion of antigen-reactive T cells in oral tolerance. Nature 376 : 177-180. Cousins, R. J., Filter, L. J. and Ziegler, E. E. ( 1996 ) Present knowledge in nutrition. 7th ed. International Life Science Institute-Nutrition Foundation. 293-306 Daphne, C. T. and Yannis, T. ( 2006 ) Immunomodulation : the future cure for allergic disease. Ann. N. Y. Acad. Sci. 1088 : 100-115. Dardalhon, V., Schubart, A. S., Reddy, J., Meyers, J. H., Monney, L., Sabatos, C. A., Ahuja, R., Nguyen, K., Freeman, G. J., Greenfield, E. A., Sobel, R. A. and Kuchroo, V. K. ( 2005 ) CD226 is specifically expressed on the surface of Th1 cells and regulates their expansion and effector functions. J. Immunol. 175 : 1558-1565. Derewenda, U., Li, J., Derewenda, Z., Dauter, Z., Mueller, G. A., Rule, G. S. and Benjamin, D. C. ( 2002 ) The crystal structure of a major dust mite allergen Der p 2, and its biological implications. J. Mol. Biol. 318 : 189-197. Faria, A. M. C. and Weiner, H. L. ( 2005 ) Oral tolerance. Immunological Reviews 206 : 233-259. Faria, A. M. C. and Weiner, H. L. ( 2006 ) Oral tolerance: Therapeutic implication for autoimmune disease. Clin. Dev. Immunol. 13 : 143-157. Finamore, A., Roselli, M., Merendino, N., Nobili, F., Vignolini, F. and Mengheri, E. ( 2003 ) Zinc deficiency suppresses the development of oral tolerance in rats. J. Nutr. 133 : 191-198. Fontenot, J. D., Gavin, M. A. and Rudensky, A. Y. ( 2003 ) Foxp3 programs the development and function of CD4+CD25+ regulatory T cells. Nat. Immunol. 4 : 330-336. Francis, J. N. and Durham, S. R. (2004) Adjuvants for allergen immunotherapy: experimental results and clinical perspectives. Curr. Opin. Allergy Clin. Immunol. 4 : 543-548. Gajiwala, K. S. and Burley, S. K. ( 2000 ) Winged helix proteins. Curr. Opin. Struct. Biol. 10 : 110-116. Gregori, S., Giarratana, N., Smiroldo, S., Uskokovic, M. and Adorini, L. ( 2002 ) A 1a,25-dihydroxyvitamin D3 analog enhances regulatory T cells and arrests autoimmune diabetes in NOD mice. Diabetes 51 : 1367-1374. Hales, B. J., Shen, H. D. and Thomas, W. R. ( 2000 ) Cytokine responses to Der p 1 and Der p 7: House dust mite allergens with different IgE binding acivities. Clin. Exp. Allergy 30 : 934-943. Hales, B. J., Hazell, L. A., Smith, W. and Thomas, W. R. ( 2002 ) Genetic variations of Der p 2 allergens: effects on T-cell responses and IgE binding. Clin. Exp. Allergy 32 : 1461-1467. Hallquist, N. A., Khoo, C. and Cousins, R. J. ( 1996 ) Lipopolysaccharide regulates cysteinerich intestinal protein, a zinc-finger protein, in immune cells and plasma. J. Leukoc. Biol. 59 : 172-177. Hamelmann, E., Vella, A. T., Oshiba, A., Kappler, J. W., Marrack, P. and Gelfand, E. W. ( 1997 ) Allergic airway sensitization induces T cell activation but not airway hyperresponsiveness in B cell-deficient mice. Proc. Natl. Acad. Sci. USA 94 : 1350-1355. Hansen, G., Yeung, V. P., Berry, G., Umetsu, D. T. and DeKruyff, R. H. ( 2000 ) Vaccination with heat-killed Listeria as adjuvant reverses established allergen-induced airway hyperreactivity and inflammation: role of CD8+ T cells and IL-18. J. Immunol. 164 : 223-230. Herz, U., Renz, H. and Wiedermann, U. ( 2004 ) Animal models of type I allergy using recombinant allergens. Methods 32 : 271-280. Heymann, P. W., Chapman, M. D., Aalberse, R. C., Fox, J. W. and Platts-Mills, T. A. ( 1989 ). Antigenic and structural analysis of group II allergens ( Der f II and Der p II ) from house dust mites ( Dermatophagoides spp ). J. Allergy Clin. Immunol. 83 : 1055-1067. Higgins, P. J. and Weiner, H. L. ( 1988 ) Suppression of experimental autoimmune encephalomyelitis by oral administration of myelin basic protein and its fragments. J. Immunol. 140 : 440-445. Hoffjan, S., and Ober, C. ( 2002 ) Present status on genetic studies of asthma. Curr. Opin. Immunol. 14 : 709-717. Holt, P. G., Warner, L. A. and Mayrhofer, G. ( 1981 ) Macrophages as effector of T suppression: T-lymphocyte-dependent macrophage-mediated suppression of mitigen-induced blastogenesis in the rat. Cell Immunol. 63 : 57-70. Horn, B. R., Robin, E. D., Theodore, J. and Van Kessel, A. ( 1975 ) Total eosinophil counts in the management of bronchial asthma. N. Engl. J. Med. 292 : 1152-1155. Inada, S., Yoshino, S., Haque, M. A., Ogata, Y. and Kohashi, O. ( 1997 ) Clonal anergy is a potent mechanism of oral tolerance in the suppression of acute antigen-induced arthritis in rats by oral administration of the inducing antigen. Cell Immunol. 175 : 67-75. Infuhr, D., Crameri, R., Lamers, R. and Achatz, G. ( 2005 ) Molecular and cellular targets of anti-IgE antibodies. Allergy 60 : 977-985. Jin, B., Scott, J. L., Vadas, M. A. and Burns, G. F. ( 1989 ) TGF beta down-regulates TLiSA1 expression and inhibits the differentiation of precursor lymphocytes into CTL and LAK cells. Immunology 66 : 570-576. Kam, K. L. and Hsieh, K. H. ( 1994 ) Comparison of three in vitro assays for serum IgE with skin testing in asthmatic children. Ann. Allergy 73 : 329-336. Kanangat, S., Blair, P., Reddy, R., Daheshia, M., Godfrey, V., Rouse, B. T., Wilkinson, E. and Deheshia, M. (1996) Disease in the scurfy mouse is associated with overexpression of cytokine genes. Eur. J. Immunol. 26 : 161-165. Kay, A. B. and Klion, A. D. ( 2004 ) Anti-interleukin-5 therapy for asthma and hypereosinophilic syndrome. Immunol. Allergy Clin. North Am. 24 : 1320-1326. Khattri, R., Kasprowicz, D., Cox, T., Mortrud, M., Appleby, M. W., Brunkow, M. E., Ziegler, S. F. and Ramsdell, F. ( 2001 ) The amount of scurfin protein determines peripheral T cell number and responsiveness. J. Immunol. 167 : 6312-6320. Kim, H. J., Hwang, S. J., Kim, B. K., Jung, K. C. and Chung, D. H. ( 2006 ) NKT cells play critical roles in the induction of oral tolerance by inducing regulatory T cells producing IL-10 and transforming growth factor b, and by clonally deleting antigen-specific T cells. Immunology 118 : 101-111. Kon, O. M., Sihra, B. S., Loh, L. C., Barkans, J., Compton, C. H., Barnes, N. C., Larche, M. and Kay, A. B. The effect of an anti-CD4 monoclonal antibody, keliximab, on peripheral blood CD4+ T-cells in asthma. Eur. Respir. J. 18 : 45-52. Lanningham-Foster, L., Green, C. L., Langkamp-Henken, B., Davis, B. A., Nguyen, K. T., Bender, B. S. and Cousins, R. J. ( 2002 ) Overexpression CRIP in transgenic mice alters cytokine patterns and the immune response. Am. J. Physiol. Endocrinol. Metab. 282 : 1197-1203. Lee, G. G., Demarquoy, J., Jackson, M. J. and O’Brien W. E. ( 1994 ) Molecular cloning and characterization of a murine LPS-inducuble cDNA. J. Immunol. 152 : 5758-5767. Levenson, C. W., Shay, N.F. and Cousins, R. J. ( 1994 ) Cloning and initial characterization of the promoter region of the rat cysteine-rich intestinal protein gene. Biochem. J. 303 : 731-736. Levenson, C. W., Shay, N. F., Hempe, J. M. and Cousins, R. J. ( 1994 ) Expression of cysteine-rich intestinal protein in rat intestine and transfected cells is not zinc dependent. J. Nutr. 124 : 13-17. Mantyjavi, R., Rautiainen, J. and Virtanen, T. ( 2000 ) Lipocalins as allergens. Biochim. Biophys. Acta. 1482 : 308-317. Martin, J. G., Suzuki, M., Ramos-Barbon, D. and Isogai, S. ( 2004 ) T cell cytokines: animal models. Paediatr. Respir. Rev. 5 : S47-51. Melamed, D. and Friedman, A. ( 1993 ) Direct evidence for anergy in T lymphocytes tolerized by oral administration of ovalbumin. Eur. J. Immunol. 23 : 935-942. Meyer, C. H., Bond, J. F., Chen, M. S. and Kasaian, M. T. ( 1994 ) Comparison of the levels of the major allergens Der p I and Der p II in standardized extracts of the house dust mite, Dermatophagoides pteronyssinus. Clin. Exp. Allergy 24 : 1041-1048. Miller, A., Lider, O., Roberts, A. B., Sporn, M. B. and Weiner, H. L. ( 1992 ) Suppressor T cells generated by oral tolerization to myelin basic protein suppress both in vivo and in vitro immune response by the release of transforming growth factor beta after antigen-specific triggering. Proc. Natl. Acad. Sci. USA 89 : 421-425. Mu, H. H. and Sewell, W. A. ( 1994 ) Regulation of DTH and IgE responses by IL-4 and IFN-gamma in immunized mice given pertussis toxin. Immunology 83 : 639-645. Nagatani, K., Sagawa, K., Komagata, Y. and Yamamoto, K. ( 2004 ) Peyer’s patch dendritic cells capturing oral antigen interact with antigen-specific t cells and induce gut-homing CD4+CD25+ regulatory T cells in Peyer’s patches. Ann. N. Y. Acad. Sci. 1029 : 366-370. Nakamura, K., Kitani, A. and Strober, W. ( 2001 ) Cell contact-dependent immunosuppression by CD4+CD25+ regulatory T cells is mediated by cell surface-bound transforming growth factor beta. J. Exp. Med. 194 : 629-644. Nelson, H. S. ( 2001 ) The importance of allergens in the development of asthma and the persistence of symptoms. Dis. Mon. 47 : 5. Nelson, H. S. ( 2004 ) Advances in upper airway disease and allergen immunotherapy. J. Allergy Clin. Immunol. 113 : 635-642. Neurath, M. F., Fuss, I., Kelsall, B. L., Presky, D. H., Waegell, W. and Strober, W. ( 1996 ) Experimental granulomatous colitis in mice is abrogated by induction of TGF-beta-mediated oral tolerance. J. Exp. Med. 183 : 2605-2616. Nobili, F., Vignolini, F., Figus, E. and Mengheri, E. (1997) Treatment of rats with dexamethasone and thyroxine protects against zinc deficiency-induced intestinal damage. J. Nutr. 127: 1807-1813. Novak, N., Kraft, S. and Bieber, T. ( 2001 ) IgE receptor. Curr. Opin. Immunol. 13 : 721-726. Oida, T., Zhang, X., Goto, M., Hachimura, S., Totsuka, M., Kaminogawa, S. and Weiner, H. L. ( 2003 ) CD4+CD25- T cells that express latency-associated peptide on the surface suppress CD4+CD45RBhigh-induced colitis by a TGF-beta-dependent mechanism. J. Immunol. 170 : 2516-2522. Ovsyannikova, I. G., Vailes, L. D., Li, Y., Heymann, P. W. and Chapman, M. D. ( 1994 ) Monoclonal antibodies to group II Dermatophagoides spp. allergens: murine immune response, epitope analysis, and development of a two-site ELISA. J. Allergy Clin. Immunol. 94 : 537-546. Pende, D., Bottino, C., Castriconi, R., Cantoni, C., Marcenaro, S., Rivera, P., Spaggiari, G. M., Dondero, A., Carnemolla, B., Reymond, N., Mingari, M. C., Marc Lopez, M., Moretta, L. and Morettac, A. ( 2005 ) PVR (CD155) and Nectin-2 (CD112) as ligands of the human DNAM-1 (CD226) activating receptor: involvement in tumor cell lysis. Mol. Immunol. 42 : 463-469. Pe´rez-Alvarado, G. C., Kosa, J. L., Louis, H. A., Beckerle, M. C., Winge, D. R. and Summers, M. F. ( 1996 ) Structure of cysteine-rich intestinal protein, CRIP. J. Mol. Bio. 257 : 153-174. Platts-Mills, T. A., Vervloet, D., Thomas, W. R., Aalberse, R. C. and Chapman, M. D. ( 1997 ). Indoor allergens and asthma: report of the Third International Workshop. J. Allergy Clin. Immunol. 100 : S2-24. Reymond, N., Imbert, A., Devilard, E., Fabre, S., Chabannon, C., Xerri, L., Farnarier, C., Cantoni, C., Bottino, C., Moretta, A., Dubreuil, P. and Lopez, M. ( 2004 ) DNAM-1 and PVR regulate monocyte migration through endothelial junctions. J. Exp. Med. 199 : 1331-1341. Russo, M., Nahori, M. A., Lefort, J., Gomes, E., A. de Castro Keller, A. de C., Rodriguez, D., Ribeiro, O. G., Adriouch, S., Gallois, V., Faria, A. M. and Vargaftig, B. B. ( 2001 ) Suppression of asthma-like responses in different mouse strains by oral tolerance. Am. J. Respir. Cell Mol. Biol. 24: 518–526. Santos, L. M., al-Sabbagh, A., Londono, A. and Weiner, H. L. ( 1994 ) Oral tolerance to myelin basic protein induces regulatory TGF-beta secreting T cells in Peyer’s patches of SJL mice. Cell Immunol. 157 : 439-447. Sato, M. N., Carvalho, A. F., Silva, A. O., MacIel, M. Jr, Fusaro, A. E. and Duarte, A. J. ( 1998 ) Oral tolerance induced to house dust mite extract in naive and sensitized mice. evaluation of immunoglobulin G anti-immunoglobulin E autoantibodies and IgG-IgE complexes. Immunology 95 : 193-199. Shi, H. N., Scott, M. E., Stevenson, M. M. and Koski, K. G. ( 1998 ) Energy restriction and zinc deficiency impair the function of murine T cells and antigen-presenting cells during gastrointestinal nematode infection. J. Nutr. 128 : 20-27. Shibuya, A., Campbell, D., Hannum, C., Yssel, H., Franz-Bacon, K., McClanahan, T., Kitamura, T., Nicholl, J., Sutherland, G. R., Lanier, L. L. and Phillips, J. H. ( 1996 ) DNAM-1, a novel adhesion molecule involved in the cytolytic function of T lymphocytes. Immunity 4 : 583-581. Shibuya, A., Lanier, L. L. and Phillips, J. H. ( 1998 ) Protein kinase C is involved in the regulation of both signaling and adhesion mediated by DNAX accessory molecule-1 J. Immunol. 161 : 1671-1676. Shibuya, K., Shirakawa, J., Kameyama, T., Honda, S., Tahara-Hanaoka, S., Miyamoto, A., Onodera, M., Sumida, T., Nakauchi, H., Miyoshi, H. and Shibuya, A. ( 2003 ) CD226 (DNAM-1) is involved in lymphocyte function-associated antigen 1 costimulatory signal for naive T cell differentiation and proliferation. J. Exp. Med. 198 : 1829-1839. Shimizu, J. and Sakaguchi, S. ( 1998 ) Immunologic self-tolerance maintained by CD25+CD4+ naturally anergic and suppressive T cells: Induction of autoimmune disease by breaking their anergic/suppressive state. Int. Immunol. 10 : 1969-1980. Simioni, P. U., Fernandes, L. G., Gabriel, D. L. and Tamashiro, W. M. ( 2004 ) Induction of systemic tolerance in normal but not in transgenic mice through continuous feeding of ovalbumin. Scand. J. Immunol. 60 : 257-266. Steinke, J. W. ( 2004 ) Anti-interleukin-4 therapy. Immunol. Allergy Clin. North Am. 24 : 599-614. Strobel, S. and Mowat, A. M. ( 1998 ) Immune responses to dietary antigens: oral tolerance. Immunol. Today 19 : 173-181. Sun, C. M., Hall, J. A., Blank, R. B., Bouladoux, N., Oukka, M., Mora, J. R. and Belkaid, Y. ( 2007 ) Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid. J. Exp. Med. 204. Sunyer, J., Anto, J. M., Sabria, J., Roca, J., Morell, F., Rodriguez-Roisin, R. and Rodrigo, M. J. ( 1995 ) Relationship between serum IgE and airway responsiveness in adults with asthma. J. Allergy Clin. Immunol. 95 : 699-706. Takahashi, T., Kuniyasu, Y., Toda, M., Sakaguchi, N., Itoh, M., Iwata, M., Shimizu, J. and Sakaguchi, S. ( 1998 ) Immunologic self-tolerance maintained by CD25+CD4+ naturally anergic and suppressive T cells: Induction of autoimmune disease by breaking their anergic/suppressive state. Int. Immunol. 10 : 1969-1980. Tan, P. H., Sagoo, P., Chan, C., Yates, J. B., Campbell, J. Beutelspacher, S. C., Foxwell, B. M. J., Lombardi, G. and George, A. J. T. ( 2005 ) Inhibition of NF-kB and oxidative pathways in human dendritic cells by antioxidative vitamins generates regulatory T cells. J. Immunol. 174 : 7633-7644. Taylor, S. F., Cottey, R. J., Zander, D. S. and Bender, B. S. ( 1997 ) Influenza infection of b2- microglobulin-deficient (b2m2/2) mice reveals a loss of CD41 T cell functions with aging. J. Immunol. 159 : 3453-3459. Tao, D., Shangwu, L., Qun, W., Yan, L., Wei, J., Junyan, L., Feili, G. and Jinquan T. ( 2005 ) CD226 expression deficiency causes high sensitivity to apoptosis in NKT cells from patients with systemic lupus erythematosus. J. Immunol. 174 : 1281-1290. Till, S. J., Francis, J. N., Nouri-Aria, K. and Durham, S. R. ( 2004 ) Mechanism of immunotherapy. J. Allergy Clin. Immunol. 113 : 1025-1034. Thomas, W. R., Smith, W. A., Hales, B. J., Mills, K. L. and O'Brien, R. M. ( 2002 ) Characterization and immunobiology of house dust mite allergens. Int. Arch. Allergy Immunol. 129 : 1-18. Thompson, H. S. and Staines, N. A. ( 1986 ) Prevention of collagen-induced arthritis by oral administration of encapsulated type II collagen. Clin. Exp. Immunol. 64 : 581-586. Tsai, J. J., Shen, H. D. and Chua, K. Y. ( 2000 ) Purification of group 2 Dermatophagoides pteronyssinus allergen and prevalence of its specific IgE in asthmatics. Int. Arch. Allergy Immunol. 121 : 205-210. van Halteren, A. G., van der Cammen, M. J., Cooper, D., Savelkoul, H. F., Kraal, G. and Holt, P. G. (1997) Regulation of antigen-specific IgE, IgG1, and mast cell responses to ingested allergen by mucosal tolerance induction. J. Immunol. 159 : 3009-3015. Wachholz, P. A. and Durham, S. R. ( 2004 ) Mechanism of immunotherapy : IgG revisited. Curr. Opin. Allergy Clin. Immuunol. 4 : 313-318. Walker, M. R., Kasprowicz, D. J., Gersuk, V. H., Benard, A., Van-Landeghen, M., Buckner, J. H. and Ziegler, S. F. ( 2003 ) Induction of FoxP3 and acquisition of T regulatory activity by stimulated human CD4+CD25- T cells. J. Clin. Invest. 112 : 1437-1443. Weiss, K. B., Gergen, P. J. and Wagener, D. K. ( 1993 ) Breathing better or wheezing worse? The changing epidemiology of asthma morbidity and mortality. Annu. Rev. Public Health. 14 : 491-513. Wells, H. G. ( 1911 ) Studies on the chemistry of anaphylaxis. III. Experiments with isolated proteins, especially those of hen’s egg. J. Inf. Dis. 9 : 147-151. Wiedermann, U., Jahn-Schmid, B., Lindblad, M., Rask, C., Holmgren, J., Kraft, D. and Ebner, C. ( 1999 ) Suppressive versus stimulatory effects of allergen/cholera toxoid (CTB) conjugates depending on the nature of the allergen in a murine model of type I allergy. Int. Immunol. 11 : 1717-1724. Williams, C. M. and Galli, S. J. ( 2000 ) The diverse potential effector and immunoregulatory roles of mast cells in allergic disease. J Allergy Clin Immunol. 105 : 847-859. Woolcock, A. J. and Peat, J. K. ( 1997 ) Evidence for the increase in asthma worldwide. Ciba Found Symp. 206 : 122-134. Yang, S., Bi, Y., He, Y., Xie, L., He, L., Xiong, J., Deng, T., Zhou, G., Liu, J., Hu, C., Zhang, X., Jin, Y., Gong, F., Jin, B. and Tan, J. ( 2005 ) V alpha 24-invariant NKT cells from patients with allergic asthma express CCR9 at high frequency and induce Th2 bias of CD3+ T cells upon CD226 engagement. J. Immunol. 175 : 4914-4926. Yagi, H., Nomura, T., Nakamura, K., Yamazaki, S., Kitawaki, T., Hori, S., Maeda, M., Onodera, M., Uchiyama, T., Fujii, S. and Sakaguchi, S. ( 2004 ) Crucial role of FOXP3 in the development and function of human CD25+CD4+ regulatory T cells. Int.Immunol. 16 : 1643-1656. Zhang, J. Z., Lee, C. S. Y., Lider, O. and Weiner, H. L. ( 1991 ) Suppression of diabetes in NOD mice by oral administration of procine insulin. Proc. Natl. Acad. Sci. USA 88 : 10252-10256. Zhang, X., Izikson, L., Liu, L. and Weiner, H. L. ( 2001 ) Activation of CD25+CD4+ regulatory T cells by oral antigen administration. J. Immunol. 167 : 4245-4253. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28902 | - |
dc.description.abstract | 塵蹣 ( Dermatophagoides pteronyssinus ) 是引起國人過敏性氣喘的主要過敏原,約80% ~ 90% 塵蹣過敏患者對塵蹣蛋白第二類蛋白質 ( Dp2 ) 產生過敏反應。當經口服食多量過敏原而引起免疫不反應性稱為口服耐受性,由本研究室先前結果已知,連續七天餵食塵蹣蛋白有效誘發口服耐受性,減緩過敏性氣喘模式小鼠的過敏免疫反應,且微陣列基因分析顯示小腸細胞 CRIP3 和 CD226 基因表現顯著增加。因此,本實驗欲探討口服耐受性的影響機制,分析短期攝取塵蹣蛋白對後續的腹腔注射致敏和支氣管刺激時與免疫調節相關基因的影響。結果顯示,連續七天口服過敏原 Dp2 後,小腸免疫組織皮耶氏體淋巴結的 CRIP3、CD226、Foxp3之 mRNA 表現量顯著增加、脾臟細胞 IFN-g 分泌量顯著上升,顯示免疫調節傾向第一型免疫反應。在後續的致敏中 CD226 雖反而下降,但是調節型 T 細胞的轉錄因數 Foxp3 mRNA 的表現量在致敏過程中仍持續增高,因而脾臟與 Peyer’s patch 表現 CD4+CD25+ 的調節型 T 細胞也顯著上升,顯示餵食大量過敏原經由腸道免疫產生較多調節型免疫細胞,可影響脾臟免疫細胞的分化與調節。因此,後續的致敏過程中脾臟細胞 IL-4 和 IL-5 皆顯著下降,調節型細胞激素 TGF-b1 也隨致敏漸增,且比氣喘組顯著較高。口服耐受組在腹腔致敏時使脾臟細胞 Dp2 特異性增生反應顯著下降,而血清總 IgE 抗體也顯著降低,後續支氣管 Dp2 刺激下仍顯著低於過敏氣喘組,顯示口服耐受性顯著抑制過敏原特異性的增生和減少總過敏抗體的生成。當進行支氣管刺激時,口服耐受組肺部呼吸道阻力 ( airway hyperresponsiveness ) 也顯著下降,肺沖洗液的 IL-4 和 IL-13 皆有顯著下降,IL-5 與 eotaxin 也有下降趨勢,顯示過敏免疫反應受到抑制。同時,血清 Dp2 特異性 IgE 抗體也有下降趨勢。因此,本研究結果顯示,短期餵食塵蹣蛋白可改變 CRIP3、CD226 和 Foxp3 的基因表現,而口服耐受組在致敏過程中仍有較高的 Foxp3 表現,並誘發調節型 T 細胞產生,抑制過敏免疫反應,使短期的餵食可達到長期免疫調節的效果。 | zh_TW |
dc.description.abstract | The dust mite is a major allergen for allergic asthma, an airway chronic inflammatory disease. About 80 ~ 90% of patients who react to mite extract react specifically to dust mite protein group 2, Dp2. Oral tolerance is a state of immunological unresponsiveness to allergen induced by feeding. Our previous study demonstrated that oral tolerance was induced efficiently by continuous feeding of Dp2 for seven days. Therefore, to investigate mechanisms of oral tolerance used in asthma, the mRNA expression and immune response in the process of oral tolerance induction, three trials including a short-term oral administration of Dp2, an added three intra-peritoneal (IP) boosting and an extra intra-tracheal (IT) challenge, were conducted. In the short-term feeding trial, the tolerance group significantly enhanced mRNA expressions of CRIP3, CD226 and Foxp3 in Peyer’s patch (PP) and Dp2-stimulated IFN-g secretion from splenocytes, indicating a Th1 dominant immunomodulation by oral induction. Although CD226 became lower in oral tolerance group after IP boosting and IT challenge, Foxp3, a transcriptional factor in regulatory T cell, remained significantly higher during the sensitization process. The CD4+CD25+ level, a regulatory T cell marker, in splenocytes and PP were also significantly higher suggesting that regulatory immune cells were induced and differentiation of splenocytes might be affected by oral tolerance. Therefore, IL-4 and IL-5 secretion were suppressed, and TGF-b1 secretion was increased in oral tolerance group during the following sensitization. The Dp2-stimulated proliferation of splenocyte and serum total IgE level decreased significantly in oral tolerance group after IP boosting. Airway hypersensitivity, Th2 cytokine such as IL-4 and IL-13 in BALF, both serum total IgE and Dp2-specific IgE levels significantly decreased in the tolerance group after IT challenge. In summary, short-term oral administration of Dp2 altered long term intestinal gene expression such as Foxp3, and induced more regulatory T cells and suppressed allergic immune responses to achieve the long-term tolerance outcome. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T00:28:42Z (GMT). No. of bitstreams: 1 ntu-96-R94b47303-1.pdf: 1596840 bytes, checksum: c01351baa423b05cb4ec8d371a9f8b78 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 目 錄
口試委員會審定書………………………………………………………………….….i 中文摘要.…………………………………………………………….……..….……....ii 英文摘要……………………………………………………….…..…….……………iii 目錄………………………………………………….…………………………...….….I 圖目錄……………………………………….………………………………….……VII 表目錄……………………………….…………………………………...…………...IX 縮寫對照表…………………………………………………………………………...XI 第一章 序論 第一節 文獻回顧……………………………………………...………………...1 一、過敏性氣喘 (一) 簡介…………………………………………………………...…..1 (二) 過敏性氣喘的定義……………………………………………….1 (三) 過敏性氣喘的致病機轉………………………………………….2 (四) 過敏性氣喘的動物模式………………………………………….4 (五) 過敏性氣喘的預防與治療方法………………………………….5 二、口服耐受性 (一) 簡介……………………………………………………………….7 (二) 誘發口服耐受性的可能機制…………………………………….8 (三) 營養素對口服耐受性的影響……………………………….…....9 (四) 口服耐受性於治療方面的應用………………………………….9 三、塵蹣過敏原 (一) 簡介……………………………………………………………...10 (二) Dp………………………………………………………………...10 四、誘發口服耐受性之相關基因 (一) 前人實驗結果…………………………………………………...12 (二) CRIP3…………………………………………………………….13 (三) CD226………………………………………………………..…..15 (四) Foxp3……………………………………………………………..16 第二節 研究目的與動機………………………………………………….…...17 第二章 實驗材料與分析方法 第一節 前言………………………………………………………….………...18 第二節 實驗材料與分析方法……………………………………….………...19 一、Dp2 之生產與純化 (一) 來源…………………………………………………..………….19 (二) 菌種解凍、確認蛋白質表現…………………………………….19 (三) 菌種準備……………………………..………………………….20 (四) 發酵槽準備……………………………………………..……….20 (五) 發酵流程………………………………………………………...20 (六) 蛋白質純化……………………………………………………...22 (七) 蛋白質分析……………………………………………………...22 (八) 蛋白質電泳……………………………………………………...23 二、動物實驗設計 (一) 實驗流程………………………..……………………………….24 (二) 實驗分組與分析指標………………………………………..….24 (三) 動物飼養………………………………………………………...26 (四) 致敏方式………………………………………………………...26 (五) 氣管內刺激……………………………………………………...27 (六) 口服耐受性之建立…………………………………………..….27 三、動物犧牲及實驗材料收集與分析 (一) 血清樣品收集………………………………………………..….27 (二) 呼吸道過度反應測試…………………………………..……….28 (三) 動物犧牲………………………………………………………...28 (四) 肺沖洗液取得…………………………………………..……….29 (五) 皮耶氏體細胞取得與培養……………………………………...29 (六) 脾臟細胞取得與培養………………………………………..….30 (七) 脾臟細胞增生測定……………………………………………...31 (八) 脾臟細胞與皮耶氏體細胞表面標記分析……………………...31 (九) 皮耶氏體細胞總 mRNA 抽取與處理…………………………32 (十) 反轉錄………………………………………………..………….33 (十一) 聚合脢鏈鎖反應……………………………………………...33 (十二) DNA 電泳…………………………………………………….35 (十三) RNA 電泳……………………………………………………..35 (十四) Dp2 特異性抗體分析……………………………………..….36 (十五) 非特異性抗體分析……………………………………..…….38 (十六) 細胞激素分泌量測定………………………………………...39 四、統計分析……………………………………………………………..…41 第三章 實驗結果 第一節 短期口服過敏原對初次致敏小鼠免疫反應的影響 ( 試驗一 ) 一、小腸淋巴組織皮耶氏體之 mRNA 表現……………………………..42 二、血清 Dp2 特異性抗體……………………………………………..…42 三、血清總抗體………………………………………………………….…43 四、脾臟細胞增生反應………………………………………………….…43 五、脾臟細胞與皮耶氏體細胞表面標記……………………………….…43 六、脾臟細胞激素……………………………………………………….…44 七、本節結論……………………………………………………………….44 第二節 短期口服過敏原對致敏小鼠免疫反應的影響 ( 試驗二 ) 一、小腸淋巴組織皮耶氏體之 mRNA 表現……………………………..51 二、血清 Dp2 特異性抗體………………………………………………..51 三、血清總抗體…………………………………………………………….51 四、脾臟細胞增生反應…………………………………………………….52 五、脾臟細胞與皮耶氏體細胞表面標記………………………………….52 六、脾臟細胞激素………………………………………………………….52 七、本節結論……………………………………………………………….53 第三節 短期口服過敏原對致敏小鼠誘發氣喘後免疫反應的影響 ( 試驗三 ) 一、小腸淋巴組織皮耶氏體之 mRNA 表現……………………………..60 二、肺部呼吸道阻力……………………………………………………….60 三、肺沖洗液之細胞激素…………………………….……………………61 四、血清 Dp2 特異性抗體…………………………………………..……61 五、血清總抗體…………………………………………………………….61 六、脾臟細胞增生反應…………………………………………………….62 七、脾臟細胞與皮耶氏體細胞表面標記………………………………….62 八、脾臟細胞激素………………………………………………………….62 九、本節結論……………………………………………………………….63 第四章 討論與總結 第一節 綜合討論 一、對氣喘模式的致敏動物誘發口服耐受性………………………….…72 二、呼吸道刺激對過敏反應的影響…………………………………….…73 三、CRIP3 表現與過敏氣喘模式誘發口服耐受性的關係………………73 四、CD226 表現與過敏氣喘模式誘發口服耐受性的關係……………...74 五、調節型 T 細胞在口服耐受性中所扮演的角色……………………..76 第二節 總結……………………………………………………………………77 第五章 參考文獻………………………………………………………………..79 ※ 附表 ………………………………………………………………………………93 | |
dc.language.iso | zh-TW | |
dc.title | 塵蹣蛋白致敏小鼠短期攝取塵蹣蛋白產生口服耐受性的機制探討 | zh_TW |
dc.title | Study on oral Tolerance Induced by Short-term Feeding of Dp2 Protein to Mice sensitized and Challenged with Dp2 Protein | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 江伯倫,吳文勉,郭敏玲,李昆達 | |
dc.subject.keyword | 塵蹣,口服耐受性,調節型 T 細胞,過敏氣喘, | zh_TW |
dc.subject.keyword | Dp2,oral tolerance,regulatory T cell,allergic asthma, | en |
dc.relation.page | 95 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-26 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 微生物與生化學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-96-1.pdf 目前未授權公開取用 | 1.56 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。