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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林萬寅 | |
| dc.contributor.author | Chiou-Ling Huang | en |
| dc.contributor.author | 黃秋鈴 | zh_TW |
| dc.date.accessioned | 2021-06-13T00:19:12Z | - |
| dc.date.available | 2012-08-09 | |
| dc.date.copyright | 2011-08-09 | |
| dc.date.issued | 2011 | |
| dc.date.submitted | 2011-08-05 | |
| dc.identifier.citation | 1. Skoog, D.; Holler, F.; Nieman, T. In principle of instrumental analysis, 5th ed.; Harcourt Brace college publishers, 1998; pp 355-376.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28718 | - |
| dc.description.abstract | We have studied the enhancement in CL for luminol-KIO4 system caused by chromium(III) ion using flow injection analysis. For the KIO4/ luminol CL systm﹐about 100-fold increase in CL intensity was observed upon addition of 150 μM Cr(III) at pH 13. The effect of concentration of reagents (chromium(III)﹐luminol﹐potassium periodate)﹐pH﹐flow rate﹐and modes of reagent mixing on CL emission were also investigated and optimized.
In emission spectrum, the CL maximum occurred at 429 nm. So the CL is caused by luminol in Cr(III)/ KIO4/ luminol system. When the dissolved oxygen was removed from the solution by purging with nitrogen﹐the CL decreased by 20 %. This might indicate that superoxide ion played a minor role in this CL reaction. In order to know the mechanism of the CL of Cr(III)/ KIO4/ luminol system, we tested a series of free radical scanvengers for their effect on CL emission. The CL intensity decreased greatly in the presence of these scavengers. These results showed that in addition to •O2－, 1O2 and •OH also participated in CL reaction. The CL-enhancement may result from the reaction of KIO4 and dissolved oxygen in strong alkaline solution to produce reactive oxygen species (ROS) in the prescence of catalytic Cr3+. However, the major reason for CL-enhancement would be the formation of LuH-Cr-IO4 complex that increases the oxidation of luminol. By using the CL system, we tested 44 compounds for their effect on the CL emission, including amino acids, polyphenol, catecholamine, nitrogenous bases, vitamins, etc. The results showed that the system has high selectivity for tryptophan. Furthermore, we found that common antioxidants such as benzenediols and catecholamines showed inhibiting effect on our CL system because these compounds might eliminate •O2－. On the contrary, the CL emission increased at low concentration of benzenetriol because of the generation of more •O2－ upon oxidation of benzenetriol .Nevertheless, the CL emission decreased at high concentration. Finally we did a series of optimization for the best inhibitory effect of hydroquinone and catechol, to achieve low detection limits of 1.02 nM and 0.96 nM respectively. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T00:19:12Z (GMT). No. of bitstreams: 1 ntu-100-R98223114-1.pdf: 1434960 bytes, checksum: 429d8eeadcc1e28b228efbcfd34a0a84 (MD5) Previous issue date: 2011 | en |
| dc.description.tableofcontents | 謝誌............................................................................................................. I
摘要........................................................................................................... III Abstract........................................................................................................V 目錄...........................................................................................................VII 圖目錄.........................................................................................................X 表目錄......................................................................................................XIV 第一章 緒論 1-1化學發光簡介.........................................................................................1 1-1.1化學發光的發展............................................................................1 1-1.2化學發光的原理............................................................................3 1-1.3化學發光在分析上的應用..............................................................4 1-1.4 Luminol化學發光系統介紹............................................................5 1-1.5 IO4-為氧化劑之化學發光系統介紹..................................................7 1-1.6金屬離子催化劑增強luminol之化學發光反應................................7 1-1.7活性含氧物質對luminol化學發光系統之影響................................8 1-2流動注入分析法.....................................................................................9 1-3研究動機...............................................................................................11 第二章 實驗部分 2-1實驗藥品...............................................................................................12 2-2 實驗儀器..............................................................................................15 2-3儀器架設...............................................................................................16 2-4溶液配製...............................................................................................18 第三章 結果與討論 3-1試劑選擇................................................................................................19 3-1.1金屬離子的選擇...........................................................................19 3-1.2氧化劑的選擇...............................................................................20 3-2 Cr(III)/KIO4/ luminol發光系統.................................................................21 3-2.1流速對訊號強度的影響.................................................................21 3-2.2各種試劑濃度對化學發光的影響...................................................22 3-2.2a Luminol濃度對化學發光強度的影響.....................................22 3-2.2b Cr(III)濃度對化學發光強度的影響.........................................23 3-2.2c酸鹼度對化學發光強度的影響...............................................24 3-2.2d過碘酸鉀對化學發光強度的影響............................................25 3-3試劑的組成對化學發光的影響.................................................................26 3-3.1單�雙管流路對化學發光強度的影響.............................................26 3-3.2試劑配置對化學發光強度的影響....................................................27 3-3.3各試劑對化學發光強度的影響........................................................29 3-4發光光譜的量測......................................................................................33 3-5溶氧對化學發光的影響...........................................................................34 3-6金屬螯合劑對化學發光的影響.................................................................35 3-7自由基消滅劑對化學發光的影響..............................................................39 3-7.1氫氧自由基消滅劑的測試...............................................................40 3-7.2單態氧與超氧離子消滅劑的測試....................................................44 3-8 Cr(III)/KIO4/ luminol發光系統機制之探討................................................49 3-9 Cr(III)/KIO4/ luminol化學發光的應用.......................................................51 3-9.1胺基酸的偵測................................................................................51 3-9.2生化分子的篩選............................................................................54 3-9.3 Trihydroxybenzene的偵測..............................................................57 3-9.3a沒食子酸 (gallic acid) ...........................................................57 3-9.3b焦性沒食子酸 (pyrogallol) ....................................................59 3-9.4維生素C的偵測—抗壞血酸(ascorbic acid) ......................................61 3-9.5 Dihydroxybenzene的偵測...............................................................63 3-9.5a對苯二酚(hydroquinone) ........................................................63 3-9.5b鄰苯二酚(catechol) ...............................................................65 3-9.6兒茶酚胺(catecholamine)的偵測......................................................67 3-9.6a多巴胺(dopamine) .................................................................70 3-9.6b左多巴 (L-DOPA).................................................................72 3-9.6c正腎上腺素 (Norepinephrine)、腎上腺素 (Epinephrine) ............74 3-9.7 Dihydroxybenzene的抑制最佳化.....................................................76 3-9.7a對苯二酚(hydroquinone)的抑制最佳化....................................78 3-9.7b鄰苯二酚(catechol)的抑制最佳化............................................84 第四章 結論與未來展望.........................................................................90 第五章 參考資料.....................................................................................92 | |
| dc.language.iso | zh-TW | |
| dc.subject | 色胺酸 | zh_TW |
| dc.subject | 流動注入分析法 | zh_TW |
| dc.subject | 活性含氧物質 | zh_TW |
| dc.subject | 苯二酚 | zh_TW |
| dc.subject | 兒茶酚胺 | zh_TW |
| dc.subject | 苯三酚 | zh_TW |
| dc.subject | 三價鉻離子 | zh_TW |
| dc.title | 三價鉻離子增強Luminol-KIO4之化學發光性質研究與應用 | zh_TW |
| dc.title | Enhancement of Chemiluminescence of the Luminol-KIO4
System by Cr(III) and Its Applications | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 99-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 劉春櫻,林金全 | |
| dc.subject.keyword | 流動注入分析法,三價鉻離子,活性含氧物質,苯二酚,兒茶酚胺,苯三酚,色胺酸, | zh_TW |
| dc.subject.keyword | flow injection analysis,chromium(III) ion,reactive oxygen species,benzenediol,catecholamines,benzenetriol,tryptophan, | en |
| dc.relation.page | 94 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2011-08-05 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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