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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 羅竹芳(Chu-Fang Lo),郭光雄(Guang-Hsiung Kou) | |
dc.contributor.author | Tsan-Chi Chen | en |
dc.contributor.author | 陳贊吉 | zh_TW |
dc.date.accessioned | 2021-06-13T00:18:35Z | - |
dc.date.available | 2012-07-31 | |
dc.date.copyright | 2007-07-31 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-27 | |
dc.identifier.citation | Chang PS, Lo CF, Wang YC, Kou GH (1996) Identification of white spot syndrome associated baculovirus (WSSV) target organs in shrimp, Penaeus monodon, by in situ hybridization. Dis Aquat Org 27:131-139
Chen LL, Leu JH, Huang CJ, Chou CM, Chen SM, Wang CH, Lo CF, Kou GH (2002a) Identification of a nucleocapsid protein (VP35) gene of shrimp white spot syndrome virus and characterization of the motif important for targeting VP35 to the nuclei of transfected insect cells. Virology 293:44-53 Chen LL, Wang HC, Huang CJ, Peng SE, Chen YG, Lin SJ, Chen WY, Dai CF, Yu HT, Wang CH, Lo CF, Kou GH (2002b) Transcriptional analysis of the DNA polymerase gene of shrimp white spot syndrome virus. Virology 301:136-147 Chou HY, Huang CY, Wang CH, Kou GH, Lo CF (1995) Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimp in Taiwan. Dis Aquat Org 23:165-173 Flegel TW (1997) Special topic review: major viral diseases of the black tiger prawn (Penaeus monodon) in Thailand. World J Microbiol and Biotechnol 13:433-442 Flegel TW (2001) The New Wave, Proceedings of the Special Session on Sustainable Shrimp Culture, Aquaculture 2001. The World Aquaculture Society, Baton Rouge, LA, USA, pp. 190-214 Flegel TW and Pasharawipas T (1998). Viral accommodation: a new concept for crustaceans response to viral pathogens. In: Flegel TW (eds.),Advances in Shrimp Biotechonology. National Center for Genetic Engineering and Biotechnology, Bangkok, Thailand, pp. 245-250 Hasson KW, Lightner DV, Poulos BT, Redman RM, White BL, Brock JA (1995) Taura syndrome in Penaeus vannamei: demonstration of a viral etiology. Dis Aquat Org 23:115-126 Horwitz MS, Ilic A, Fine C, Rodriguez E, Sarvetnick N (2003) Coxsackievirus-mediated hyperglycemia is enhanced by reinfection and this occurs independent of T cells. Virology 314(2):510-20. Huang J, Song XL, Yu J, Yang CH (1995a) Baculoviral hypodermal and hematopoietic necrosis - study on the pathogen and pathology of the explosive epidemic disease of shrimp. Mar Fish Res 16:1-10 Huang J, Yu J, Song XL, Kong J, Yang CH (1995b) Studies on fine structure, nucleic acid, polypeptide and serology of hypodermal and hematopoietic necrosis baculovirus of penaeid shrimp. Mar Fish Res 16:11-23 Huber SC, MacKintosh C, Kaiser WM (2002) Metabolic enzymes as targets for 14-3-3 proteins. Plant Mol Biol 50: 1053–63. Inouye K, Miwa S, Oseko N, Nakano H, Kimura T, Momoyama K, Hiraoka M (1994) Mass mortalities of cultured kuruma shrimp Penaeus japonicus in Japan in 1993: Electron microscopic evidence of the causative virus. Fish Pathol 29:149-158 Ishitani R, Chuang DM (1996) Glyceraldehyde-3-phosphate dehydrogenase antisense oligodeoxynucleotides protect against cytosine arabinonucleoside-induced apoptosis in cultured cerebellar neurons. Neurobiology 93:9937–41. Karunasagar I, Otta SK, (1997) Histopathological and bacteriological study of white spot syndrome of Penaeus monodon along the west coast of India. Aquaculture 153:9-13 Kinne O (1963) The effect of temperature and salinity on marine and brackish water animals: I. Temperature. Oceanogr Marine Biol Annu Rev, 1:301-340 Kuo CM, Hsu CJ, Li CY (1996) Hyperglycemic effects of dopamine in tiger shrimp, Penaeus monodon, Aquaculture 135:161-172 Kurtz J, Franz K (2003) Evidence for memory in invertebrate immunity. Nature 425:37 Leu JH, Chang CC, Wu JL, Hsu CW, Hirono I, Aoki T, Juan HF, Lo CF, Kou GH, Huang HC (2007) Comparative analysis of differentially expressed genes in normal and white spot syndrome virus infected Penaeus monodon. BMC Genomics 16;8:120. Leu JH, Tsai JM, Wang HC, Wang AH, Wang CH, Kou GH, Lo CF (2005) The unique stacked rings in the uncleocapsid of the white spot syndrome virus virion are formed by the major structural protein VP664, the largest viral structural protein ever found. J Virol 79:140-149 Lightner DV (1996) A handbook of shrimp pathology and diagnostic procedures for diseases of penaeid shrimp World Aquaculture Society, Baton Rouge, Louisiana Lightner DV, Poulos BT, Redman RM, Mari J, Bonami JR (1992) New developmentsin penaeid virology: Application of biotechnology in research and disease diagnosisfor shrimp viruses of concern in the Americas. In: Fulks W, Main K (eds) Proceedings of the Asian Interchange Program Workshop on the Diseases of Cultured Penaeid Shrimp, Asian Interchange Program, The Oceanic Institute, Oahu, Hawaii, pp. 233-253 Lin SS, Chang SC, Wang YH, Sun CY, Chang MF (2000) Specific interaction between the hepatitis delta virus RNA and glyceraldehyde 3-phosphate dehydrogenase: an enhancement on ribozyme catalysis. Virology 271:46–57. Lo CF, Ho CH, Chen CH, Liu KF, Chiu YL, Yeh PY, Peng SE, Hsu HC, Liu HC, Chang CF, Su MS, Wang CH, Kou GH (1997) Detection and tissue tropism of white spot syndrome baculovirus (WSBV) in captured brooders of Penaeus monodon with a special emphasis on reproductive organs. Dis Aquat Org 30:53-72 Lo CF, Ho CH, Peng SE, Chen CH, Hsu HC, Chiu YL, Chen YT, Chang CF, Liu KF, Su MS, Wang CH, Kou GH (1996) Infection of white spot syndrome associated virus (WSBV) in cultured and wild-caught shrimps, crabs and other arthropods. Dis Aquat Org 27:215-225 Lo CF, Kou GH (1998) Virus-associated white spot syndrome of shrimp in Taiwan: a review. Fish Pathol 33:365-371 Lotz JM, Browdy CL, Carr WH, Frelier PF, Lightner DV (1995) USMSFP suggested procedures and guidelines for assuring the specific pathogen status of shrimp broodstock and seed. In: Browdy CL, Hopkins JS (eds) Swimming Through Troubled Water, Proceedings the Special Session on Shrimp Farming, Aquaculture '95. World Aquaculture Society, Baton Rouge, Louisina, USA, pp. 66-75 Marks H, Mennens M, Vlak JM, van Hulten MC (2003) Transcriptional analysis of the white spot syndrome virus major virion protein genes. J Gen Virol 84:1517-1523 Momoyama K, Hiraoka M, Nakano H, Koube H, Inouye AK, Oseko N (1994) Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: Histopathological study. Fish Pathol 29:141-148 Nacar A (2007) Could pathogens be nutrients for lymphoid cells? ( in press). Nadala ECB, Tappy LM, Loh PC (1997) Yellow-head virus: a rhabdovirus-like pathogen of penaeid shrimp. Dis Aquat Org 31:141-146 Nahlik KW, Mleczko AK, Gawlik MK, Rokita HB (2003) Modulation of GAPDH expression and cellular localization after vaccinia virus infection of human adherent monocytes. Acta Biochim Pol 50:667–76. Nakano H, Koube H, Umezawa K, Momoyama K, Hiraoka M, Inouye K, Oseko N (1994) Mass mortalities of cultured kuruma shrimp, Penaeus japonicus, in Japan in 1993: Epizootiological survey and infection trials. Fish Pathol 29:135-139 Singh P, Salih M, Leddy JJ, Tuana BS (2004) The muscle-specific calmodulin-dependent protein kinase assembles with the glycolytic enzyme complex at the sarcoplasmic reticulum and modulates the activity of glyceraldehyde-3-phosphate dehydrogenase in a Ca2+/calmodulin-dependent manner. J Biol Chem 279:35176–282. Snieszko SF, (1974) The effects of environmental stress on outbreaks of infectious diseases of fishes. J. Fish Biol. 6, 197-208. Takahashi Y, Itami T, Kondo M, Maeda M, Fujii R, Tomonaga S, Supamattaya K, Boonyaratpalin S, (1994) Electron microscopic evidence of bacilliform virus infection in kuruma shrimp (Penaeus japonicas). Fish Pathol 29:121-125. Tsai JM, Wang HC, Leu JH, Hsiao HH, Wang AH, Kou GH, Lo CF (2004) Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J Virol 78:11360-11370 Tsai MF, Lo CF, van Hulten MC, Tzeng HF, Chou CM, Huang CJ, Wang CH, Lin JY, Vlak JM, Kou GH (2000a) Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus. Virology 277:92-99 Tsai MF, Yu HT, Tzeng HF, Leu JH, Chou CM, Huang CJ, Wang CH, Lin JY, Kou GH, Lo CF (2000b) Identification and characterization of a shrimp white spot syndrome virus (WSSV) gene that encodes a novel chimeric polypeptide of cellular-type thymidine kinase and thymidylate kinase. Virology 277:100-110 Van de Braak CB, Botterblom MH, Huisman EA, Rombout JH and van der Knaap WP (2002) Preliminary study on haemocyte response to white spot syndrome virus infection in black tiger shrimp Penaeus monodon. Dis Aquat Organ 2002 Aug 29;51(2):149-55. Van Etten JL, Graves MV, Muller DG, Boland W, Delaroque N (2002) Phycodnaviridae--large DNA algal viruses. Arch Virol 147:1479-1516 Van Hulten MC, Reijns M, Vermeesch AM, Zandbergen F, Vlak JM (2002) Identification of VP19 and VP15 of white spot syndrome virus (WSSV) and glycosylation status of the WSSV major structural proteins. J Gen Virol 83:257-265 Wang CH, Lo CF, Leu JH, Chou CM, Yeh PY, Chou HY, Tung MC, Chang CF, Su MS, Kou GH (1995) Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon. Dis Aquat Org 23:239-242 Wang HC, Wang HC, Leu JH, Kou GH, Wang AH, Lo CF (2007) Protein expression profiling of the shrimp cellular response to white spot syndrome virus infection. Dev Comp Immunol. 31(7):672-86. Witteveldt J, Vlak JM and van Hulten MCW (2004) Protection of Penaeus monodon against white spot syndrome virus using a WSSV subunit vaccine. Fish Shellfish Immunol 16: 571-579 Wongteerasupaya C, J. E. Vickers, S. Sriurairatana, G. L. Nash, A. Akarajamorn, V. Boonsaeng, S. Panyim, A. Tassanakajon, B. Withyachumnarnkul and T. W. Flegel (1995). A non-occluded, systemic baculov irus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. Dis Aquat Org 21: 69-77. Yang F, He J, Lin X, Li Q, Pan D, Zhang X, Xu X (2001) Complete genome sequence of the shrimp white spot bacilliform virus. J Virol 75:11811-11820 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28706 | - |
dc.description.abstract | 白點症病毒是一具有高侵襲性的蝦類病原體,俟至目前,有關於此病毒與宿主之間交互作用的研究仍極為有限。本研究首先分析病毒感染對白蝦血淋巴液中血糖、乳酸和三酸甘油酯的影響。結果發現,血淋巴液中乳酸量及三酸甘油酯量,於感染後逐漸下降,但血糖量卻在感染後呈現上揚之趨勢,12小時上升約1.74倍,推論白點症病毒藉由促進乳酸分解以利糖質新生作用進行,使得宿主血糖含量上升。本實驗也進一步以二維膠體電泳分析白點病毒感染白蝦胃部組織的蛋白質表現變化,觀察到烯醇酶 (Enolase)、醛縮酶 (Aldolase)、3-磷酸甘油醛脫氫酶 (Glyceraldehyde 3-phosphate dehydrogenase, GAPDH) 蛋白質含量確有明顯著的上升,並用西方轉印法分析烯醇酶,亦證實烯醇酶的蛋白質時序表現量亦有增加。
藉由比對白點症病毒感染前後草蝦表現序列標誌 (Expressed sequence tag, EST) 資料庫後,發現烯醇酶、醛縮酶、3-磷酸甘油醛脫氫酶基因表現量於病毒感染後上升。利用反轉錄酶-聚合酶鏈反應 (reverse transcriptase polymerase chain reaction, RT-PCR) 進行這三個基因在受到病毒感染蝦胃部組織的表現分析,亦證明證明這些基因的轉錄表現於病毒感染後的呈現明顯上升的趨勢。 本研究証實,白點症病毒感染蝦體後,會影響蝦類的醣類代謝途徑,促使血淋巴液中血糖的上升、乳酸及三酸甘油酯的下降,胃部組織細胞烯醇酶、醛縮酶、3-磷酸甘油醛脫氫酶基因表現的基因表現亦有變化,而能量代謝產物與醣類代謝相關酵素之相互關連性和其生理重要性,仍後續研究加以釐清。 | zh_TW |
dc.description.abstract | White spot syndrome virus (WSSV) is known to be a virulent pathogen to penaeid shrimp. To date, information on the interaction between WSSV and hosts are still limited. The present study analyzed the temporal changes in the hemolymph glucose, lactate and triglyceride of the white shrimp under WSSV infection. The hemolymph lactate and triglycerides decreased after the challenge, while the hemolympyh glucose showed an increasing trend, peaked at 12 hr post-challenge, 1.74-folds increase over the control. The observations suggest that the hyperglycemic response of the shrimp is consequenced partly from the remetabolism of lactate in the process of glyconeogenesis. The protein expressions in the gastric tissue of the shrimp were further performed by 2-D gel electrophoresis, and the contents of enolase, aldolase and glyceraldehydes 3-phosphate dehydrogenase (GAPDH) were all markedly elevated. The increasing trend of the temporal expression of enolase also demonstrated by West-blotting method.
The gene expression of enolase, aldolase, and GAPDH were notably increased after the WSSV infection to tiger shrimp, indicated in the differential display of expressed sequence tag of the species. These changes are further confirmed in the present study using the method of reverse transcriptase polymerase chain reaction (RT-PCR). It is therefore concluded that the carbohydrate metabolism and the expressions of enolsse, aldolase, and GAPDH genes in the gastric tissues are all influenced by WSSV infection. However, inter-relationship of the energy metabolites and the activities of carbohydrate metabolism-related enzymes, and its physiological significance remain for further elucidation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T00:18:35Z (GMT). No. of bitstreams: 1 ntu-96-R94b41014-1.pdf: 897453 bytes, checksum: d28942f4d54866cb6ab2abac6f985c1f (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 口試委員會審定書……………………………………………………i
誌謝……………………………………………………………………ii 中文摘要………………………………………………………………iii Abstract………………………………………………………………iv 目錄……………………………………………………………………v 圖目錄 ………………………………………………………………viii 表目錄…………………………………………………………………ix 前言……………………………………………………………………1 1.1 白點症病毒 ……………………………………………………1 1.2 南美白蝦簡介 …………………………………………………4 1.3 研究目的 ………………………………………………………5 1.4 糖解作用 (Glycolysis)………………………………………6 材料與方法……………………………………………………………8 2.1 實驗動物 ………………………………………………………8 2.2 實驗用海水 ……………………………………………………8 2.3 病毒製備與人工感染試驗流程 ………………………………8 2.3.1 病毒製備 ……………………………………………………8 2.3.2 人工感染試驗流程 …………………………………………9 2.4 血淋巴液之抽取 ………………………………………………10 2.5 蝦檢體病毒帶原檢測 …………………………………………10 2.5.1 檢體DNA萃取 ………………………………………………10 2.5.2 蝦類白點症病毒核酸檢測法 ………………………………11 2.6 血糖、乳酸、三酸甘油酯之測定 ……………………………11 2.6.1 乳酸 (Lactate) 之測定……………………………………12 2.6.2 血糖 (Glucose) 之測定……………………………………12 2.6.3 三酸甘油酯 (Triacylglycide) 之測定 …………………13 2.7 檢體RNA萃取……………………………………………………14 2.8 反轉錄酶-聚合酶鏈反應 (reverse transcriptase polymerase chain reation, RT-PCR) ……………………………14 2.9 基因表現時序分析 ……………………………………………15 2.10 西方轉印法 (Western blot assay) ………………………16 2.10.1 蛋白質樣本製備……………………………………………16 2.10.2 十二烷基硫酸鈉-聚丙烯醯胺膠電泳 (sodium dodecyl sulfate polyacrylaminde Gel eletrophoresis, SDS-PAGE) 分析………………………………………………………………………16 2.10.3 西方轉印法…………………………………………………17 2.11 蛋白質二維電泳………………………………………………18 2.11.1 蛋白質樣本製備……………………………………………18 2.11.2 蛋白質樣本之定量…………………………………………18 2.11.3 二維電泳之分析……………………………………………19 2.11.3.1 等電焦集電泳……………………………………………19 2.11.3.2 SDS-PAGE 分析 …………………………………………20 結果……………………………………………………………………21 1. 白點症病毒檢測…………………………………………………21 2. 白點症病毒基因時序性轉錄表現分析…………………………21 3. 白點症病毒感染後對南美白蝦血淋巴液之血糖、乳酸、三酸甘油酯含量的影響………………………………………………………22 3.1 白點症病毒感染後對白蝦血淋巴液中血糖含量的影響 ……22 3.2 白點症病毒感染對白蝦血淋巴液中乳酸含量之影響 ………22 3.3 白點症病毒感染後對白蝦血淋巴液中三酸甘油酯含量之影響………………………………………………………………………23 4. 白蝦胃部組織感染白點症病毒後二維電泳分析烯醇酶、醛縮酶及3-磷酸甘油醛脫氫酶蛋白質表現之差異…………………………23 5. 感染白點症病毒後西方轉印法分析白蝦胃部組織烯醇酶之蛋白質時序性表現差異分析………………………………………………24 6. 感染白點症病毒之白蝦胃部烯醇酶、醛縮酶及3-磷酸甘油醛脫氫酶基因表現時序分析………………………………………………25 討論……………………………………………………………………26 結論……………………………………………………………………32 參考文獻………………………………………………………………33 附錄……………………………………………………………………52 | |
dc.language.iso | zh-TW | |
dc.title | 白點症病毒感染後白蝦血淋巴液中血糖、乳酸、三酸甘油酯分析與烯醇酶、醛縮酶、3-磷酸甘油醛脫氫酶及基因表現之分析 | zh_TW |
dc.title | Analysis on the changes of hemolymph glucose, lactate and triglycerides, and the gene expressions of enolase, aldolase and glyceraldehyde 3-phosphate dehydrogenase in white shrimp, Litopenaus vannamei under WSSV infection | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.advisor-orcid | ,郭光雄(ghkou@ntu.edu.tw) | |
dc.contributor.oralexamcommittee | 王重雄(Chung-Hsiung Wang),郭欽明(Ching-Ming Kuo) | |
dc.subject.keyword | 白點症病毒,白蝦,能量代謝產物,醣類代謝相關酵素,二維膠體電泳分析法,反轉錄酶,-聚合酶,鏈反應, | zh_TW |
dc.subject.keyword | white shrimp,WSSV,energy metabolites,metabolism-related enzymes, | en |
dc.relation.page | 52 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-27 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 動物學研究研究所 | zh_TW |
顯示於系所單位: | 動物學研究所 |
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