請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28356完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 鍾美珠 | |
| dc.contributor.author | Shao-An Fang | en |
| dc.contributor.author | 方紹安 | zh_TW |
| dc.date.accessioned | 2021-06-13T00:05:58Z | - |
| dc.date.available | 2009-07-31 | |
| dc.date.copyright | 2007-07-31 | |
| dc.date.issued | 2007 | |
| dc.date.submitted | 2007-07-28 | |
| dc.identifier.citation | Adam-Blondon A-F, Sevignac M, Bannerot H, Dron M. (1994) SCAR, RAPD and RFLP markers linked to a dominant gene (Are) conferring resistance to anthracnose in common bean. Theor Appl Genet 88: 865-870.
Aggarwal R-K, Brar D-S, Khush G-S. (1997) Two new genomes in the Oryza complex identified on the basis of molecular divergence analysis using total genomic DNA hybridization. Mol Gen Genet 254:1-12. Alfenito M-R and Birchler J-A. (1993) Molecular characterization of a maize B chromosome centric sequence. Genetics 135:589-597. Appels R, Reddy P, MacIntyre C-L, Moran L-B. (1989) The molecular-cytogenetic analysis of grasses and its application to studying relationships among species of the Triticeae. Genome 31: 122-133. Ananiev E-V, Phillips R-L, Rines H-W. (1998) Chromosome-specific molecular organization of maize (Zea mays L.) centromeric regions. Proc Natl Acad Sci U.S.A. 95:13073-13078. Andersen J-R and Lubberstedt T. (2003) Functional markers in plants. Trends Plant Sci 8:554-560. Aswidinnoor H, Nelson R-J, Dallas J-F, Mcintyre C-L, Leung H, Gustafson J-P. (1991) Cloning and characterization of repetitive DNA sequences from genomes of Oryza minuta and Oryza australiensis. Genome 34:790-798. Barret P, Delourme R, Foisset N, Renard M. (1998) Development of a SCAR sequence characterized amplified region marker for molecular tagging of the dwarf BREIZH (Bzh) gene in Brassica napus L. Theor Appl Genet 97: 828-833. Beismann H, Barker H, Karp A, Speck T. (1997) AFLP analysis sheds light on distribution of two Salix species and their hybrid along a natural gradient. Mol Ecol 6:989-993. Bennett M-D, Smith J-B. (1991) Nuclear DNA amounts in angiosperms. Philosophical Transactions of the Royal Society of London Series B-Biological Sciences 334: 309-345. Brar D-S and Khush G-S. (1997) Alien introgression in rice. Plant Mol Biol. 35:35-47. Calderini O, Pupilli F, Paolocci F, Arcioni S. (1997) A repetitive and species-specific sequence as a tool for detecting the genome contribution in somatic hybrids of the genus Medicago. Theor Appl Genet 95 : 734-740. Cao Q, Lu B-R, Xia H, Rong J, Sala F, Spada A, Grassi F. (2006) Genetic diversity and origin of weedy rice (Oryza sativa f. spontanea) populations found in north-eastern china revealed by simple sequence repeat (SSR) markers. Ann Bot 98:1241-1252. Chang, T-T., Qu S-H, Pathak M-D, Ling K-C, Kauffman H-E. (1975) The search for disease and insect resistance in rice germplasm. In: Frankel OH, Hawkes JG (eds) Crop genetic resources for today and tomorrow. Cambridge University Press, Cambridge, England, pp 183-200. Cheng Z, Dong F, Langdon T, Ouyang S, Buell C-R, Gu M, Blattner F-R, Jiang J. (2002) Functional rice centromeres are marked by a satellite repeat and a centromere-specific retrotransposon. Plant Cell 14:1691-1704. Cheng Y-Y, Fang S-A, Lin Y-C, Chung M-C. (2007) A repetitive sequence specific to Oryza species with BB genome and abundant in Oryza punctata Kotschy ex Steud. Botanical Studies (in press). Chung M-C, Lee Y-I, Cheng Y-Y, Chou Y-J, Lu C-F. (2007) Chromosomal polymorphism of ribosomal 1 genes in the genus Oryza. Theor Appl Genet (in press). Cordesse F, Reddy A-S, De Kochko A, Kiefer M-C, Delseny, M. (1991) Characterization of repeated DNA sequences specific to different rice genomes In: Rice Genetics II, International Rice Research Institute, Manila, pp 401-407. Das M, Bhattacharya S, Pal A. (2005) Generation and characterization of SCARs by cloning and sequencing of RAPD products: a strategy for species-specific marker development in bamboo. Ann Bot 95: 835-841. Daud H-M, Gustafson J-P. (1996) Molecular evidence for Triticum speltoides as a B-genome progenitor of wheat (Triticum aestivum). Genome 39:543-548. Dawe R-K, Richardson E-A, Zhang X. (2005) The simple ultrastructure of the maize kinetichore fits a two domain model. Cytogenet Genome Res 109:128-133. Devos K-M. (2005) Updating the ‘crop circle’. Current Opinion in Plant Biology 8:155–162. Devos K-M and Gale M-D. (1992) The use of random amplified polymorphic DNA markers in wheat. Theor Appl Genet 84:567-572. Dong F, Miller J-T, Jackson S-A, Wang G-L, Ronald P-C, Jiang J. (1998) Rice (Oryza sativa) Centromeric regions consist of complex DNA. Proc Natl Acad Sci U.S.A. 95:8135-8140. Dvořák J and Zhang H-B. (1992a) Application of molecular tools for study of the phylogeny of diploid and polyploid taxa in Triticeae. Hereditas, 116: 37-42. Dvořák J and Zhang H-B. (1992b) Reconstruction of the phylogeny of the genus Triticum from variation in repeated nucleotide sequences. Theor Appl Genet 84: 419-429. Echt C-S, Erdahl L-A, McCoy T-J. (1992) Genetic segregation of random amplified polymorphic DNA in diploid cultivated alfalfa. Genome, 35: 84-87. Flavell R. (1980) The molecular characterization and organization of plant chromosomal DNA sequences. Annu Rev Plant Physiol 31: 569-596. Fransz P-F, Alonso-Blanco C, Liharska T-B, Peeters A-J-M, Zabel P, de Jong J-H. (1996) High-resolution physical mapping in Arabidopsis thaliana and tomato by fluorescence in situ hybridization to extended DNA fibres. The Plant Journal 9:421-430. Galasso I, Schmidt T, Pignone D, Heslop-Harrison J-S. (1995) The molecular cytogenetics of Vigna unguiculata (L.) Walp : the physical organization and characterization of 18S-5.8S-25 S rRNA genes, 5S rRNA, telomere-like sequences, and a family of centromeric repetitive DNA sequences. Theor Appl Genet 91:928-935. Gall J-G and Pardue M-L. (1969) Formation and detection of RNA-DNA hybrid molecules in cytological preparations. Proc Natl Acad Sci U.S.A. 63:378-383 Ge S, Sang T, Lu B-R, Hong D-Y. (1999) Phylogeny of rice genomes with emphasis on origins of allotetraploid species. Proc Natl Acad Sci U.S.A. 96: 14400-14405. Goff S_A, Ricke D, Lan T-H, Presting G, Wang R, Dunn M, Glazebrook J, Sessions A, Oeller P, Varma H, Hadley D, Hutchison D, Martin C, Katagiri F, Lange B-M, Moughamer T, Xia Y, Budworth P, Zhong J, Miguel T, Paszkowski U, Zhang S, Colbert M, Sun WL, Chen L, Cooper B, Park S, Wood TC, Mao L, Quail P, Wing R, Dean R, Yu Y, Zharkikh A, Shen R, Sahasrabudhe S, Thomas A, Cannings R, Gutin A, Pruss D, Reid J, Tavtigian S, Mitchell J, Eldredge G, Scholl T, Miller R-M, Bhatnagar S, Adey N, Rubano T, Tusneem N, Robinson R, Feldhaus J, Macalma T, Oliphant A, Briggs S. (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science 296:92-100. Hernandez P, Martin A, Dorado G. (1999) Development of SCARs by direct sequencing of RAPD products: A practical tool for the introgression and marker assisted selection of wheat. Mol Breed 5: 245-253. Heslop-Harrison J-S, Brandes A, Taketa S, Schmidt T, Vershinin A-V, Alkhimova E-G, Kamm A, Katsiotis A, Doudrick R-L, Schwarzacher T, Kubis S, Kumar A, Pearce S-R, Flavell A-J, Harrison G-E (1997) The chromosomal distribution of retrotransposon-like elements in higher plants and its implication for genome evolution. Genetica 100 : 197-204. International Rice Genome Sequencing Project et al. (2005) The map-based sequence of the rice genome. Nature 436:793-800. Iyengar G-A-S, Sen S-K. (1978) Nuclear DNA content of several wild and cultivated Oryza species. Environ Exp Bot 18: 219-224. Jiang J, Birchler J-A, Parrott W-A, Dawe R-K. (2003) A molecular view of plant centromeres. Trends Plant Sci 8:570-575. John H-A, Birnstiel M-L, Jones K-W. (1969) RNA-DNA hybrids at the cytological level. Nature 223:582-587. Kamm A, Galasso I, Schmidt T, Heslop-Harrison J-S. (1995) Analysis of a repetitive DNA family from Arabidopsis arenosa and relationships between Arabidopsis species. Plant Mol Biol 27:853-862. Khush G-S. (1997) Origin, dispersal, cultivation and variation of rice. Plant Mol Biol 35:25-34. Kiefer-Meyer M-C, Reddy A-S, Delseny M. (1995) Characterization of a dispersed repetitive DNA sequence associated with the CCDD genome of wild rice. Genome 38:681-688. Ko J-M, Do G-S, Suh D-Y, Seo B-B, Shin D-C, Moon H-P. (2002) Identification and chromosomal organization of two rye genome-specific RAPD products useful as introgression markers in wheat. Genome 45:157-164. Kulikova O, Geurts R, Lamine M, Kim D-J, Cook D-R, Leunissen J, de Jong H, Roe B-A, Bisseling T. (2004) Satellite repeats in the functional centromere and pericentromeric heterochromatin of Medicago truncatula. Chromosoma 113: 276-283. Kunze R, Saedler H, Lönnig W-E (1997) Plant transposable elements. In : Callow JA (ed) Adv Bot Res 27 : 331-470 Langer-Safer P-R, Levine M, Ward D-C. (1982) Immunological method for mapping genes on Drosophila polytene chromosomes. Proc Natl Acad Sci U.S.A. 79:4381-4385. Lapitan N-L-V., Sears R-G, Gill B-S. (1984) Translocations and other karyotypic structural changes in wheat × rye hybrids regenerated from tissue culture. Theor Appl Genet 68: 547-554. Lapitan N-L-V., Sears R-G, Gill B-S. (1988) Amplification of repeated DNA sequences in wheat × rye hybrids regenerated from tissue culture. Theor Appl Genet 75: 381-388. Li C-B, Zhang D-M, Ge S, Lu B-R, Hong D-Y. (2001) Differentiation and inter-genomic relationships among C, E and D genomes in the Oryza officinalis complex (Poaceae) as revealed by multicolor genomic in situ hybridization. Theor Appl Genet 103:197-203. Lim K-Y, Skalicka K, Koukalova B, Volkov R-A, Matyasek R, Hemleben V, Leitch A-R, Kovarik A. (2003) Dynamic changes in the distribution of a satellite homologous to intergenic 26-18S rDNA spacer in the evolution of Nicotiana. Genetics 166: 1935-1946. Liu Z-L, Dong Y-Z, Liu B. (2002) Characterization of two groups of low-copy and specific DNA sequences isolated from chromosome 7B of common wheat. J. Integr Plant Biol 8 (abstract) Ma J, Devos K-M, Bennetzen J-L. (2004) Analyses of LTR-retrotransposon structures reveal recent and rapid genomic DNA loss in rice. Genome Res 14: 860-869. Ma, J. and S. A. Jackson. (2006). Retrotransposon accumulation and satellite amplification mediated by segmental duplication facilitate centromere expansion in rice. Genome Res. 16: 251-259. Marillia E-F and Scoles G. (1996) The use of RAPD markers in Hordeum phylogeny. Genome 39: 646-654. Martinez C-P, Arumuganathan K, Kikuchi H, Earle E-D. (1994) Nuclear DNA content of ten rice species as determined by flow cytometry. Jpn J Genet 69: 513-523. Matyasek R, Gazdova B, Fajkus J, Bezdek M. (1997) NTRS, a new family of highly repetitive DNAs specific for the T1 chromosome of tobacco. Chromosoma 106:369-379. McCarthy E-M, Liu J-D, Lizhi G, McDonald J-F. (2002) Long terminal repeat retrotransposons of Oryza sativa. Genome Biol 3:research0053.1-0053.11. McCarthy B-J and Farquhar M-N. (1972) The rate of change of DNA in evolution. Brookhaven Symp Biol 23:1-43. Moringa T. (1964) Cytogenetical investigations on Oryza species. In: IRRI (ed) Rice Genetics and Cytogenetics, Elsevier, Amsterdam, pp. 91-110. Nakajima R, Noma K, Ohtsubo H, Ohtsubo E. (1996) Identification and characterization of two tandem repeat sequences (TrsB and TrsC) and a retrotransposon (RIRE1) as genome-general sequences in rice. Genes Genet Syst 71: 373-382. Nakagahra M, Okuno K, Vaughan D (1997) Rice genetic resources: history, conservation, investigative characterization and use in Japan. Plant Mol Biol 35: 69-77. Nagaki K, Neumann P, Zhang D, Ouyang S, Buell C-R, Cheng Z, Jiang J. (2005) Structure, divergence, and distribution of the CRR centromeric retrotransposon family in rice. Mol Biol Evol 22: 845-855. Naqvi N-I and Chattoo B-B. (1996) Development of a sequence-characterized amplified region (SCAR) based indirect selection method for a dominant blast resistance gene in rice. Genome 39: 26-30. Noma K, Nakajima R, Ohtsubo H, Ohtsubo E (1997) RIRE1, a retrotransposon from wild rice Oryza australiensis. Genes Genet Syst 72:131-40. Oakey H-J, Gibson L-F, George A-M. (1998) Co-migration of RAPD-PCR amplicons from Aeromonas hydrophila. FEMS Microbiol Lett 164:35-38. Ohtsubo H, Ohtsubo E (1994) Involvement of transposition in dispersion of tandem repeat sequences (TrsA) in rice genomes. Mol Gen Genet 245: 449-455. Oka H-I. (1988) Origin of Cultivated Rice. Elsevier, Amsterdam, 255 pp. Oka H-I, Morishima H, Sano Y, Koizumi T. (1977) Observation of rice species and accompanying savanna plants on the southern fringe of Sahara desert. Report of study-tour in West Africa. (http://www.shigen.nig.ac.jp/rice/oryzabase/top/ top.jsp) Olmstead R-G , Palmer J-D. (1994) Chloroplast DNA systematics: a review of methods and data analysis. Am J Bot 81:1205-1224. Paran I and Michelmore R-W. (1993) Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce. Theor Appl Genet 85: 985-993. Ried T, Baldini A, Rand T-C, Ward D-C. (1992) Simultaneous visualization of seven different DNA probes by in situ hybridization using combinatorial fluorescence and digital imaging microscopy. Proc Natl Acad Sci U.S.A. 89:1388-1392. Reddy A-S, Kiefer-Meyer M-C, Delseny M. (1993) Characterization of new variants of a satellite DNA from Oryza officinalis, specific for the CC genome of wild rice. Genome 36:750-761. Ren F, Lu B-R, Li S, Huang J, Zhu Y. (2003) A comparative study of genetic relationships among the AA-genome Oryza species using RAPD and SSR markers. Theor Appl Genet 108:113-120. Richards E-J and Ausubel F-M. (1988) Isolation of a higher eukaryotic telomere from Arabidopsis thaliana. Cell 53:127-136. Rokka V-M, Clark M-S, Knudson D-L, Pehu E, Lapitan N-L-V. (1998) Cytological and molecular characterization of repetitive DNA sequences of Solanum brevidens and Solanum tuberosum. Genome, 41:487-494. Schmidt T, Kubis S, Katsiotis A, Jung C, Heslop-Harrison J-S. (1998) Molecular and chromosomal organization of two repetitive DNA sequences with intercalary locations in sugar beet and other Beta species. Theor Appl Genet 97: 696-704. Schröck E, du Manoir S, Veldman T, Schoell B, Wienberg J, Ferguson-Smith M-A, Ning Y, Ledbetter D-H, Bar-Am I, Soenksen D, Garini Y, Ried T. (1996) Multicolor spectral karyotyping of human chromosomes. Science 273:494-497. Shcherban A-B and Vershinin A-V. (1992) The stretched BamHI fragment of the barley genome containing highly repetitive DNA sequences. Genetika (Moscow) 28:15-21. Sitch L-A, Dalmacio R-D, Romero G-O. (1989) Crossability of wild Oryza species and their potential use for improvement of cultivated rice. Rice Genet Newslett 6:58-60. Skinner D-Z. (1992) A PCR-bssed method of identifiying species-specific repeated DNAs. Biotechniques 13:210-214. Svitashev S, Bryngelsson T, Vershinin A, Pedersen C, Säll T, von Bothmer R. (1994) Phylogenetic analysis of the genus Hordeum using repetitive DNA sequences. Theor Appl Genet 89: 801-810. Svitashev S, Salomon B, Bryngelsson T, von Bothmer R. (1996) A study of 28 Elymus species using repetitive DNA sequences. Genome 39: 1093-1101. Svitashev S, Bryngelsson T, Li X, Wang R-R-C. (1998) Genome-specific repetitive DNA and RAPD markers for genome identification in Elymus and Hordelymus. Genome 41: 120-128. Thomas H-M, Harper J-A, Meredith M-R, Morgan W-G, King I-P. (1997) Physical mapping of ribosomal DNA sites in Festuca arundinacea and related species by in situ hybridization. Genome 40:406-410. Tsujimoto H, Gill B-S. (1991) Repetitive DNA sequences from polyploid Elymus trachycaulus and the diploid progenitor species: detection and genomic affinity of Elymus chromatin added to wheat. Genome 34:782-789. Uozu S, Ikehashi H, Ohmido N, Ohtsubo H, Ohtsubo E, Fukui K. (1997) Repetitive sequences: cause for variation in genome size and chromosome morphology in the genus Oryza. Plant Mol Biol 35:791-799. Vaughan D-A, Morishimay H, Kadowaki K. (2003) Diversity in the Oryza genus. Curr Opin Plant Biol 6:139-146. Vidal J-R, Delavault P, Coarer M, Defontaine A. (2000) Design of grapevine (Vitis vinifera L.) cultivar-specific SCAR primers for PCR fingerprinting. Theo Appl Genet 101:1194-1201. Visser R-G-F, Hoekstra H, van der Leij F-R, Pijnacker L-P, Witholt B, Feenstra W-J. (1998) In situ hybridization to somatic metaphase chromosomes of potato. Theo Appl Genet 76:420-424. Vos P, Hogers R, Bleeker M, Reijans M, Lee T-V-D, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M. (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407-4415. Wang Z-X., Kurata N, Saji S, Katayose Y, Minobe Y. (1995) A chromosome 5-specific repetitive DNA sequence in rice (Oryza sativa L). Theo Appl Genet 90:907-913. Weeden N-F, Timmerman G-M, Hemmat M, Kneen B-E, Lodhi M-A. (1992) Inheritance and reliability of RAPD markers. Applications of RAPD Technology to Plant Breeding. Joint Plant Breeding Symposia Series. Madison, WI: Crop Science Society of America, 12-17. Wiegant J, Kalle W, Mullenders L, Brookes S, Hoovers J-M-N, Dauwerse J-G, van Ommen G-J-B, Raap A-K. (1992) High-resolution in situ hybridization using DNA halo preparations. Hum Mol Genet 1:587-591. Welsh J and McClelland M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucl Acids Res 18: 7213-7218. Williams J-G-K, Hanafey M-K, Rafalski J-A, Tingey S-V. (1993) Genetic analysis using random amplified polymorphic DNA markers. Methods Enzymol 218: 704-740. Williams J-G-K, Kubelik A-R, Livak K-J, Rafalski J-A, Tingey V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531-6535. Wing R-A, Ammiraju J-S, Luo M, Kim H, Yu Y, Kudrna D, Goicoechea J-L, Wang W, Nelson W, Rao K, Brar D, Mackill D-J, Han B, Soderlund C, Stein L, SanMiguel P, Jackson S. (2005) The Oryza map alignment project: the golden path to unlocking the genetic potential of wild rice species. Plant Mol Biol 59:53-62. Wu H-K, Chung M-C, Wu T-Y, Ning C-N, Wu R. (1991) Localization of specific repetitive DNA sequences in individual rice chromosomes. Chromosoma 100:330-338. Wu J-Y, Wu H-K, Chung M-C. (2002) Co-dominant RAPD markers closely linked with two morphological genes in rice (Oryza sativa L.). Bot Bull Acad Sin 43:171-180. Wu T-Y and Wu R. (1992) A novel repetitive DNA sequence in the genus Oryza. Theor Appl Genet 84:136-144. Wu T-Y and Wu R. (1994a) Identification of a rice BB genome type specific repetitive DNA sequence. Rice Genet Newslett 11:161-164. Wu T-Y and Wu R. (1994b) Identification of a rice CCDD genome type-specific repetitive DNA sequence. Rice Genet Newslett 11:164-167. Xiao J, Li J, Grandillo S, Ahn S-N, Yuan L, Tanksley S-D, and McCouch S-R. (1998) Identification of trait-improving quantitative trait loci alleles from wild rice relative, Oryza rufipogon. Genetics 150:899-909. Xie Y and Wu R. (1989) Rice alcohol dehydrogenase genes: anaerobic induction, organ-specific expression and characterization of cDNA clones. Plant Mol Biol 13:53-68. Xie Y and Wu R. (1990) Molecular analysis of an alcohol dehydrogenase-encoding genomic clone (Adh2) from rice. Gene 87:185-191. Xu H, Wilson D-J, Arulsekar S, Bakalinsky A-T. (1995) Sequence specific polymerase chain reaction markers derived from randomly amplified polymorphic DNA markers for fingerprinting grape (Vitis) rootstocks. J Am Soc Hortic Sci 120:714-720. Xue Y, Li J, Xu Z. (2003) Recent highlights of the China Rice Functional Genomics Program. Trends Genet 19:390-394. Yan H-H, Liu G-Q, Cheng Z-K, Li X-B, Liu G-Z, Min S-K, Zhu L-H. (2002) A genome-specific repetitive DNA sequence from Oryza eichingeri : characterization, localization, and introgression to O. sativa. Theor Appl Genet 104:177-183. Yu H-G, Hiatt E-N, Chan A, Sweeney M, Dawe R-K. (1997) Neocentromere-mediated chromosome movement in mazie. J Cell Biol 139:831-840. Zhang Y, Huang Y, Zhang L, Li Y, Lu T, Lu Y, Feng Q, Zhao Q, Cheng Z, Xue Y, Wing R-A, Han B. (2004) Structural features of the rice chromosome 4 centromere. Nucl Acids Res 32: 2023-2030. Zhang Z-B and Dvorak J. (1990) Isolation of repeated DNA sequences from Lophopyrum elongatum for detection of Lophopyrum chromatin in wheat genomes. Genome 33: 283-293. Zhao X, Kochert G. (1992) Characterization and genetic mapping of a short, highly repeated, interspersed DNA sequence from rice (Oryza sativa L). Mol Gen Genet 231:353-359. Zhao X, Kochert G. (1993a) Clusters of interspersed repeated DNA sequences in the rice genome (Oryza). Genome 36:944-953. Zhao X, Kochert G. (1993b) Phylogenetic distribution and genetic mapping of a (GGC)n microsatellite from rice (Oryza sativa L.). Plant Mol Biol 21: 607-617. Zhao X, Wu T, Xie Y, Wu R. (1989) Genome-specific repetitive sequences in the genus Oryza. Theor Appl Genet 78:201-209. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28356 | - |
| dc.description.abstract | 利用分子標記可以分辨物種基因組之組成及檢測種間雜交後代基因組中異源物種基因組滲入程度等。本論文以12種水稻為參試材料，包括栽培種和野生種，分別屬於九個物種共涵蓋六個基因組型：AA，BB，BBCC，CC，CCDD，EE。栽培種水稻參試品種中，日本晴（O. sativa, Japonica, cv. Nipponbare）為稻屬研究中經常使用的稉稻品種之一，而台農67號（O. sativa, Japonica, cv. TNG 67）為目前在台灣地區普遍栽種，為常用之育種親本和研究材料稉稻品種。由O. punctata Kotschy ex steud（W1593）分離得到兩個逢機引子擴增DNA片段（randomly amplified polymorphic DNAs，RAPD），分別命名為Opun225和Opun246。Opun225片段長度為874 bp，在基因組內為低重複數的片段，對於稻屬BB基因組型二元體物種有高度的專一性，可作為RAPD分子標誌，有效的分辨不同基因組型物種之不同族群。Opun246片段長度約為2084 bp，在O. punctata (W1593)單套基因組內約有6,320個重複，屬於一種高重複數的片段。對於稻屬B基因組型物種有高度的基因組型專一性，能有效的分辨不同基因組型物種。將Opun246序列與生物核苷序列收集資料庫中的已知序列比對，可找到少數的同為稻屬的同源序列，且大多均在其5’端約有1200 bp的同源性較高，而在3’端核苷序列的同源性較低。此外，Opun246片段75~1266 bp之間的序列與一段被註解為類似retroelements的序列之間有75％一致性。南方墨漬雜交的結果顯示，Opun246為高度重複的序列且散布在BB基因型物種之全基因組內。以螢光原位雜交（FISH）的染色體定位結果則指出，Opun246散布在BB基因組物種的染色體之異染色質區域。此外，Opun246的訊號有集中在O. punctata（BB）染色體靠近末端或中節區域的現象。然而，在四元體O. punctata（BBCC）和O. minuta（BBCC）中，Opun246只會出現在BB基因組的染色體上，且有集中在染色體末端位置的趨勢。因此認為Opun246多數分佈在靠近中節或末端附近的異染色質區域，且為一個BB基因組專一性的分子標記。 | zh_TW |
| dc.description.abstract | Molecular markers are useful in discriminating specific constituents of genome and monitoring genomic introgression of interspecific hybrids. In this study, we use 12 rice materials, including nine Oryza species, both cultivated and wild species of rice, which belong to six genome types. We isolate and characterize two genome specific RAPDs, named Opun225 and Opun246, from Oryza punctata Kotschy ex Steud (W1593), an African native wild rice. The Opun225 sequence is 874 base pair in length and a low-copy sequence present in genome and is demonstrated as a repetitive sequence specific to a diploid Oryza species with B genome. On the other hand, the Opun246 is 2084 bp in length and estimated at 6320 copies in O. punctata (W1593) haploid genome. Sequence comparison analysis by BLAST found a few homologues of Opun246 in EMBL-GenBank database. Among those homologues, a relatively high similarity was found in the position ~1200 nucleotides regions at the 5’ ends, but a low similarity in the rest of the nucleotides at the 3’ends. In addition, the Opun246 sequence at position 75~1266 nucleotides with 75% identity to a putative retroelements. The results of Southern hybridization indicated that the repetitive Opun246 sequences dispersed throughout the entire genome of specues with B genome. The results of fluorescent in situ hybridization (FISH) indicated that the repetitive Opun246 sequence dispersed to the heterochromatin throughout chromosomes in the whole genome. Furthermore, obvious Opun246 sequences were detected near the centrimeric and terminal regions at chromosomes of O. punctata (W15777). The Opun246 sequence were detected near the terminal regions on part of chromosomes, which might be restricted to B genome originated chromosomes in O. punctata (W1564) and O. minuta (W0045) with BBCC. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-13T00:05:58Z (GMT). No. of bitstreams: 1 ntu-96-R94621119-1.pdf: 4983073 bytes, checksum: 5567841366186868335c0392d9d33f11 (MD5) Previous issue date: 2007 | en |
| dc.description.tableofcontents | 中文摘要： 5
ABSTRACT 6 壹、前言： 7 貳、前人研究： 9 (一) 水稻（Oryza sativa L.）基因體之探討 9 (二) 重複性DNA序列之探討 11 (三) RAPD分子標誌的應用 12 (四) 螢光原位雜交技術（fluorescent in situ hybridization, FISH）之應用 14 参、材料方法： 16 (一) 植物材料 16 (二) 植物基因組DNA的萃取 16 (三) RAPD擴增基因組專一性DNA片段 17 (四) TA Cloning 18 (五) Slot Blot Hybridization 20 (六) 基因組重複數的測量 21 (七) 南方氏轉漬雜交反應（Southern Blot Hybridization） 22 (八) 以Sequence Characterized Amplified Region（SCAR）引子確認選殖片段之專一性 23 (九) 染色體的製備 23 (十) 螢光原位雜交（Fluorescence in situ hybridization，FISH） 24 肆、結果： 26 (一) 擴增水稻基因組專一性的RAPD： 26 (二) 專一性RAPD序列的讀序與比對分析： 26 (三) 以slot blot雜交方法分析所選殖的擴增片段在基因組內的重複數： 27 (四) 以南方氏轉漬雜交分析所選殖的RAPD片段在基因組的專一性： 28 (五) 以南方氏轉漬雜交分析選殖擴增片段在基因組內的分布情形： 30 (六) 設計SCAR引子對及對不同基因組的擴增反應： 31 (七) 利用螢光原位雜交技術標記RAPDs在染色體上的分布： 32 伍、討論： 34 (一) RAPD序列的同源性 34 (二) SCAR引子對的非專一性擴增 35 (三) 以南方氏墨漬分析分析重複序列專一性 36 (四) 重複序列於基因組內的差異 38 (五) RAPDs偏好分布於染色體上異染色質的位置 39 (六) 螢光原位雜交之解析力 40 陸、結論： 40 參考文獻： 41 表目次 表1、參試水稻種源及其基因組類型 54 表2、稻屬各物種中Opun246序列的重複數 55 表3、稻屬RAPD擴增片段衍生的基因組專一性SCAR引子對序列及PCR擴增反應的黏合溫度 56 圖目次 圖1、以逢機引子UBC225由稻屬物種基因組PCR擴增產物之膠體電泳圖 57 圖2、以逢機引子UBC246由稻屬物種基因組PCR擴增產物之膠體電泳圖 58 圖3、Opun246序列經以BLAST與生物核苷酸序列資料庫比對結果 59 圖4、以Slot blot hybridization定量分析法估算Opun225序列在不同水稻基因組中的重複數 60 圖5、以Slot blot hybridization定量分析法估算Opun246序列在不同水稻基因組中的重複數 61 圖6、以Opun225為探針和逢機引子UBC 225之RAPD擴增產物之南方氏轉漬膜雜交的結果 62 圖7、以Ogla225為探針和逢機引子UBC 225之RAPD擴增產物之南方氏轉漬膜雜交的結果 63 圖8、以Opun246為探針和逢機引子UBC 246之RAPD擴增產物之南方氏轉漬膜雜交的結果 64 圖9、以Opun246為探針和基因組DNA酶切片段解析膠體之南方氏轉漬膜雜交的結果 65 圖10、四個帶有B基因組型稻屬物種之基因組DNA經以BamHI、HindIII、PstI和XbaI共四種酵素酶切後的電泳解析膠體圖 66 圖11、以Opun225為探針和基因組DNA酶切片段解析膠體之南方氏轉漬膜雜交的結果 67 圖12、以Opun246為探針和基因組DNA酶切片段解析膠體之南方氏轉漬膜雜交的結果 68 圖13、以Opun246為探針和基因組DNA酶切片段解析膠體之南方氏轉漬膜雜交的結果 69 圖14、以RCS2為探針和基因組DNA酶切片段解析膠體之南方氏轉漬膜雜交的結果 70 圖15、以SCAR-Opun246為引子PCR擴增產物膠體電泳解析結果。箭頭標示處為相對應於Opun246的條帶 71 圖16、以螢光原位雜交（FISH）標記Opun246在O. punctata（BB，W1577）染色體上的位置 72 圖17、以螢光原位雜交（FISH）標記Opun246在O. punctata（BBCC，W1564）染色體上的位置 73 圖18、以螢光原位雜交（FISH）標記Opun246在O. minuta（BBCC，W0045）染色體上的位置 74 | |
| dc.language.iso | zh-TW | |
| dc.subject | 重複序列 | zh_TW |
| dc.subject | 螢光原位雜交技術 | zh_TW |
| dc.subject | 野生稻Oryza punctata | zh_TW |
| dc.subject | 物種專一性逢機引子多型性標誌 | zh_TW |
| dc.subject | repetitive sequence | en |
| dc.subject | fluorescent in situ hybridization (FISH) | en |
| dc.subject | species-specific RAPD | en |
| dc.subject | Oryza punctata | en |
| dc.title | 野生水稻物種B型基因組特有的RAPD之研究 | zh_TW |
| dc.title | A RAPD Specific to Rice Wild Species with B Genome | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 95-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 謝兆樞 | |
| dc.contributor.oralexamcommittee | 高燕玉,胡凱康 | |
| dc.subject.keyword | 螢光原位雜交技術,野生稻Oryza punctata,重複序列,物種專一性逢機引子多型性標誌, | zh_TW |
| dc.subject.keyword | Oryza punctata,repetitive sequence,species-specific RAPD,fluorescent in situ hybridization (FISH), | en |
| dc.relation.page | 74 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2007-07-30 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| 顯示於系所單位： | 農藝學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-96-1.pdf 未授權公開取用 | 4.87 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。