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標題: | 荷葉甲醇萃出物對肥胖大鼠之影響 The effect of lotus leaf methanolic extract on obese rat |
作者: | Yung-Fang Chiu 邱永芳 |
指導教授: | 江文章,許順堯 |
關鍵字: | 肥胖,荷葉甲醇萃出物,SD大白鼠,脂肪細胞, obesity,lotus leaf methanolic extract,SD rat,adipose cell, |
出版年 : | 2007 |
學位: | 碩士 |
摘要: | 肥胖的問題近年來在全球已是備受注目的焦點,由於肥胖易導致疾病的發生。荷葉在『本草綱目』記載荷葉服之,令人瘦劣,單服可以消陽水浮腫之氣。因此,本研究利用常見的減肥用茶-荷葉,探討是否具有減少體脂肪之功效。以60隻10週齡雄性SD 大白鼠為實驗動物模式,分為對照組與實驗組,對照組餵予Purina 5001商業配方(3.4 Kcal/g),實驗組餵予高能量飲食(HE diet)(4.5 kcal/g)。實驗組根據荷葉甲醇萃出物(LLM)的額外添加分為0% (HE)、0.45% LLM、1.59% LLM、4.03% LLM、5.38% LLM,在預養階段,使HE組的大白鼠體重較對照組重20% (p<0.05),再開始餵予荷葉甲醇萃取物四週後犧牲。實驗結果發現,荷葉甲醇萃出物並不影響大白鼠攝食量,而荷葉甲醇萃出物的給予會使高劑量的組別(4.03%、5.38%)體重較HE組顯著降低(p<0.01),實驗組(1.59%、4.03%、5.38%)可顯著使大白鼠血清TG下降,但血清TC方面,1.59%、5.38%組可使增加,與HE相較具顯著上升。血清中HDL-C在添加荷葉甲醇萃出物後,可使血清HDL-C增加,在1.59%及5.38%組與HE組具有顯著上升。在血糖方面,隨著荷葉甲醇萃出物的濃度增加,可使血液中葡萄糖濃度下降。肝臟TG在4.03%、5.38%組與HE組具有顯著差異(p<0.05)。0.45%、1.59%、4.03%、5.38%組能使肝臟TC下降。糞便TG在4.03%、5.38%組會與HE組具有顯著差異,糞便TC在添加1.59%、4.03%、5.38%組會使總膽固醇的排除增加。觀察脂肪細胞直徑發現高劑量組別(4.03%、5.38%)直徑會顯著較HE組小(p<0.05)。
關鍵字:肥胖、荷葉甲醇萃出物、SD大白鼠、脂肪細胞 Obesity has been a universal concern because it can be the cause of a lot of disease. The aim of our research is to estimate the effect of lotus leaf which is often used as diet tea in Chinese herbs on reducing body fat. According to the Compendium of Materia Medica, a classic book of Chinese herbs, lotus leaf has a curing effect on obesity. In our experiment, we divided 60 SD rats into two groups, control and experiment group. In the control group we fed the rats with Purina 5001 commercial diet (3.4kcal/g). In the experiment group we fed the rats with high-energy (HE) diet (4.5kcal/g). The methanolic extract of lotus leaf(LLM)was added into high-energy diet to feed the experiment group. We used different dosages which were 0%, 0.45%, 1.59%, 4.03% and 5.38%. Before feeding the high-energy diet, we made the experiment group body weight 20% more than the control group (p<0.05). After that, we started to feed the experiment group with LLM for 4 weeks and then sacrificed the rats. We found that feeding diet with LLM did not influence the amount of ingestion in SD rats. However, we found a significant decrease of body weight (p<0.01) in rats that were fed with LLM at dosage of 4.03%, 5.38% compared with those fed with HE diet. There was a significant decrease of serum TG in rats fed with 1.59%, 4.03%, and 5.38% LLM compared with HE diet. We also found a significant increase of serum TC in 1.59% and 5.38% LLM groups compared with HE diet. In addition, in groups of feeding 1.59% and 5.38% LLM, HDL significantly increased compared with HE group. We also found that the concentration of LLM was negatively correlated with the decrease of blood sugar. In the results of liver TG, there were significant different (p<0.05) in groups of 4.03% and 5.38% LLM compared with HE group. The liver TC decreased in groups of 0.45%, 1.59%, 4.03%, and 5.38% LLM. In the results of fecal TG, there were significant different (p<0.05) in 4.03% and 5.38% LLM groups compared with HE group. We also found that in fecal TC, there was an increase of body cholesterol elimination in groups of 1.59%, 4.03% and 5.38% LLM. Finally, we observed the diameter of adipose cells and found smaller diameter in high dosage group (4.03% and 5.38%) than in HE group (p<0.05). Key Word: obesity、lotus leaf methanolic extract (LLM)、SD rat、adipose cell |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28166 |
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顯示於系所單位: | 食品科技研究所 |
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