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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 胡哲明(Jer-Ming Hu) | |
dc.contributor.author | Chiao-Yi Cheng | en |
dc.contributor.author | 鄭喬尹 | zh_TW |
dc.date.accessioned | 2021-06-13T00:01:23Z | - |
dc.date.available | 2007-08-01 | |
dc.date.copyright | 2007-08-01 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-07-30 | |
dc.identifier.citation | 參考文獻
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Lo, H.S., Ko, W.C., Chen, W.C., Hsue, H.H., and Wu, H. (eds.) (1999) Mussaenda Linn. Flora of China Vol. 71 No. 1. Science Press, Beijing, China. pp. 283-306. Maddison, D.R. and Maddison, W.P. (2000). MacClade 4: Analysis of phylogeny and character evolution. Sinauer Associates, Inc., Sunderland, MA, USA. Masamune, G. (1939). Miscellaneous notes on the flora of eastern asia X. Trans. Nat. Hist. Soc. Formosa 29, 179-180. Massinga, P.H., Johnson, S.D., and Harder, L.D. (2005). Heteromorphic incompatibility and efficiency of pollination in two distylous Pentanisia species (Rubiaceae). Annals of Botany 95, 389-399. Matsumura, J. (1900). Mussaenda parviflora and Mussaenda macrophylla. The Botanical Magazine, Tokyo 14, 147. Matsumura, J. and Hayata, B. (1906). Journal Coll. Sci. Univ. Tokyo (Enum. Pl. Form.). 22, 108. Miquel. (1867). Mussaenda Linn. Ann. Mus. Bot. Lugt-Bat. 3, 110. Naiki, A. and Kato, M. (1999). Pollination system and evolution of dioecy from distyly in Mussaenda parviflora (Rubiaceae). Plant Species Biology 14, 217-227. Neal, P.R. and Anderson, G.J. (2004). Does the ‘old bag’ make a good ‘wind bag’?: comparison of four fabrics commonly used as exclusion bags in studies of pollination and reproductive biology. Annals of Botany 93, 603-607. Pope, M. and Oxelman, B. (2001). Inferring the history of the polyploidy Siline aegaea (Caryophyllaceae) using plastid and homoeologous nuclear DNA sequences. Molecular Phylogenetics and Evolution 20, 474-481. Razafimandimbison, S.G. and Bremer, B. (2002). Phylogeny and classification of Naucleeae s.l. (Rubiaceae) inferred from molecular (ITS, rBCL and tRNT-F) and morphological data. American Journal of Botany 89, 1027-1041. Richard, A.J. (1997). Plant breeding systems. (2nd ed.) Chapman and Hall, London, UK. Rova, J.H.E., Delprete, P.G.., Anderson L., and Albert, V.A. (2002). A trnL-F cpDNA sequence study if the Condamineeae- Rondeletieae-Sipaneeae complex with implications on the phylogeny of the Rubiaceae. American Journal of Botany 89, 145-159. Saitou, N. and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4, 406-425. Taberlet, P., Gielly, L., Pautou, G., and Bouvet, J. (1991). Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Molecular Biology 17, 1105-1109. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F., and Higgins, D.G. (1997). The CLUSTAL-X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acid Research 25, 4876-4882. Waites, A.R. and Ågren, J. (2006). Stigma receptivity and effects of prior self-pollination on seed set in tristylous Lythrum salicaria (Lythraceae). American Journal of Botany 93, 142-147. Wang, X.L., Yu, W.G., Zhou, Y.P., Cheng, H.Z., and Tian, C.E. (2007). Rapid isolation of microsatellites from genome of Mussaenda pubescens. Journal of Guangzhou University (Natural Science Edition) 6, 50-52. Weber, H. (1955). Über die Blütenkeche tropischer Rubiaceen. Akademie der Wissenschaften und der Literatur in Mainz. p. 455. fig. 455. Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalshi, J.A., and Tingey, S.V. (1900). DNA polymorphisma amplified by arbitrary primers are useful as genetic markers. Nucl. Acids Res. 18, 6531-6535. Yang, T.Y.A. (1998). Mussaenda Burm. In: Huang, T.C. et al. (eds.) Flora of Taiwan. Vol. IV.(2nd ed.) Department of Botany, NTU, Taipei, Taiwan. pp.301-303. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28126 | - |
dc.description.abstract | 茜草科的玉葉金花屬植物,常利用膨大呈現鮮艷顏色的葉狀萼片來吸引授粉者。台灣產的玉葉金花植物,其葉狀萼片有時會有大小,以及顏色上的變異,因而在分類常造成困擾。台灣地區除了蘭嶼之外,在台灣植物志第二版中被認定的玉葉金花屬植物僅有毛玉葉金花 (Mussaenda pubescens W.T. Aiton) 一種;根據觀察發現,在台灣北部地區的玉葉金花植株,通常都缺乏吸引授粉者的膨大葉狀萼片構造或是僅有少數無法完整發育的葉狀萼片,但是仍然有果實的產生;而且其形態與Masamune (1939) 發表的台北玉葉金花 (M. taihokuensis Masam.) 的描述一致。從形態上的觀察,台灣北部與南部玉葉金花的花部構造有著明顯的差異,典型的北部短花冠類型植物之花冠顏色較為淺黃,而花冠筒的長度相較於南部長花冠類型短;兩個花冠類型的花萼與花萼筒長度比值都不及M. pubescens描述的標準。另外,我們將台灣北部與南部地區所採集到不同花冠長度的玉葉金花植株樣本,利用核內ITS序列與葉綠體內基因trnT至trnF間的序列,比對已發表的玉葉金花屬植物相關序列資料,發現到不論台灣北部短花冠型或是南部長花冠型的植株樣本,均與玉葉金花(M. parviflora Miq.) 以及四國玉葉金花 (M. shikokiana Makino) 較相近,而與已發表採自香港之M. pubescens序列不同。最後,我們針對長短花冠型的玉葉金花進行交叉授粉實驗,初步的結果發現兩個花冠型之間的交配無法產生果實,這個結果也顯示台灣南北不同花冠型的玉葉金花之間可能已存在初步的生殖隔離。綜合以上結果,台灣中南部常見具有膨大萼片的玉葉金花植株,應為玉葉金花 (M. parviflora),而台灣北部地區缺乏膨大萼片的短花冠型植株應為種之階級,或可暫名為台北玉葉金花 (M. taihokuensis)。 | zh_TW |
dc.description.abstract | The enlarged and showy calyx lobes of the species of Mussaenda (Rubiaceae), have been known to attract pollinators. Variations on the size and color of Mussaenda species in Taiwan were observed, but the significance was unknown. In current taxonomic treatment by the second edition of the Flora of Taiwan, only one species was recognized in the island of Taiwan - Mussaenda pubescens Aiton. Based on the morphological observation, two forms of Mussaenda species can be found, one is mostly distributed on the northern part of Taiwan, another towards the southern part of Taiwan. The northern populations are congruent in the description of a previously recognized taxon, M. taihokuensis Masam., in having small calycophylls, and they show no sign of reproductive failures since they still set fruits in the field. Further examination shows that this northern form of Mussaenda has lighter petal color and shorter corolla tube than the southern form; and both northern and southern populations of Mussaenda have equal length of calyx teeth and calyx tube, which is incongruent with the description of M. pubescens.
Phylogenetic analyses were conducted based on nrITS and chloroplast trnT-F spacer and the results show that all sequenced individuals in Taiwan are grouped with M. parviflora Miq. and M. shikokiana Makino, and distinct from M. pubescens collected in Hong-Kong. Moreover, our data from crossed-pollination experiments for the northern and southern forms show that the crossed flowers cannot set fruits, indicating that these two forms have developed reproductive isolation even they can co-exist in the field. Based on all the results, the commonly seen Mussaenda species in middle and southern part of Taiwan should be named M. parviflora and the ones in northern part of Taiwan should be better named M. taihokuensis. | en |
dc.description.provenance | Made available in DSpace on 2021-06-13T00:01:23Z (GMT). No. of bitstreams: 1 ntu-96-R93b44003-1.pdf: 2998596 bytes, checksum: 42af8c3264030b5bbae3339b8bd3a941 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 目錄
中文摘要……………………………………………………………… i 英文摘要……………………………………………………………… ii 1、前言…………………………………………………………………1 1.1台灣產玉葉金花屬植物之分類歷史………………………………1 1.2 台灣產玉葉金花族群…………………………………………… 4 1.3 玉葉金花屬內近緣種的分類研究……………………………… 6 1.4 玉葉金花屬植物的生殖策略…………………………………… 10 1.5 研究目的………………………………………………………… 13 2、材料與方法 ……………………………………………………… 14 2.1 研究地點與樣本採集…………………………………………… 14 2.2 花部形態特徵觀察……………………………………………… 21 2.3 植物總體DNA萃取 ……………………………………………… 23 2.4 聚合酶連鎖反應………………………………………………… 24 2.5 譜系分析………………………………………………………… 27 2.6 生殖生物學調查………………………………………………… 29 3、結果 ……………………………………………………………… 32 3.1 台灣產玉葉金花族群分布……………………………………… 32 3.2 花部形態特徵…………………………………………………… 34 3.3 譜系分析………………………………………………………… 44 3.4 生殖生物學調查………………………………………………… 58 4、討論 ……………………………………………………………… 64 4.1 台灣產玉葉金花屬植物的鑑定與族群差異…………………… 64 4.2 花部形態特徵與授粉作用……………………………………… 66 4.3 台灣短花冠型玉葉金花植株的分類定位……………………… 68 參考文獻……………………………………………………………… 70 附錄…………………………………………………………………… 74 附錄1 玉葉金花葉片與葉狀萼片橫切面手繪圖…………………… 74 附錄2 移除洋玉葉金花其葉狀萼片與花冠之實驗結果…………… 75 附錄3 台灣產玉葉金花族群分布詳細整理………………………… 76 附錄4 玉葉金花DNA片段的序列資料 ……………………………… 78 附錄5 玉葉金花DNA片段的變異位置 ……………………………… 99 表目次 表1.1台灣玉葉金花屬植物分類系統整理表…………………………5 表1.2台灣玉葉金花屬植物花部器官特徵整理統計表………………9 表2.1玉葉金花採集資料表……………………………………………15 表2.2其他玉葉金花屬物種採集資料表………………………………19 表2.3人工授粉實驗地點………………………………………………20 表2.4聚合酶連鎖反應所使用於放大特定基因片段之引子序列……25 表2.5短花冠型植株 (北部缺乏膨大萼片植株) 之人工授粉處理…31 表3.1玉葉金花花部形態特徵測量值…………………………………36 表3.2玉葉金花雄蕊與雌蕊形態測量值………………………………37 表3.3自GenBank下載玉葉金花屬植物相關基因序列序號與樣本採集來源 ……………………………………………………………………46 表3.4玉葉金花DNA經由聚合酶連鎖反應後所增幅之基因片段 ……48 表3.5台灣地區玉葉金花族群的性別調查結果………………………61 表3.6短花冠型植株 (北部缺乏膨大萼片植株) 之人工授粉測試結果 ………………………………………………………………………62 圖目次 圖1.1 台灣北部類型的玉葉金花植株形態 …………………………7 圖1.2 台灣南部類型的玉葉金花植株形態 …………………………8 圖2.1 玉葉金花花部器官測量部位手繪圖 …………………………22 圖3.1 台灣地區玉葉金花族群與一月 (最冷月) 月均溫分布圖 …33 圖3.2 台灣地區玉葉金花其花冠筒形態 ……………………………38 圖3.3 玉葉金花不同部位之花部形態測量統計圖 …………………39 圖3.4 利用掃瞄式電子顯微鏡 (SEM) 觀察玉葉金花花部器官表皮細胞形態 …………………………………………………………………43 圖3.5 以nr ITS依鄰近連接分析法 (NJ) 所建構的譜系樹 ………49 圖3.6 以nr ITS依高度簡約原理 (MP) 所建構的嚴格一致性譜系樹 ………………………………………………………………………… 50 圖3.7 以nr ITS依貝葉氏導出式分析 (BI) 所建構的譜系樹 ……51 圖3.8 以trnT-trnF依鄰近連接分析法 (NJ) 所建構的譜系樹……52 圖3.9 以trnT-trnF依高度簡約原理 (MP) 所建構的嚴格一致性譜系樹 ………………………………………………………………………53 圖3.10 以trnT-trnF依貝葉氏導出式分析 (BI) 所建構的譜系樹 ………………………………………………………………………… 54 圖3.11 以nr ITS與trnT-trnF依鄰近連接分析法 (NJ) 所建構的譜系樹 ……………………………………………………………………55 圖3.12 以nr ITS與trnT-trnF依高度簡約原理 (MP) 所建構的嚴格一致性譜系樹 …………………………………………………………56 圖3.13 以nr ITS與trnT-trnF依貝葉氏導出式分析 (BI) 所建構的譜系樹 …………………………………………………………………57 圖3.14 玉葉金花之花粉管萌發測試…………………………………60 | |
dc.language.iso | zh-TW | |
dc.title | 臺灣產玉葉金花屬植物之親緣關係與生殖生物學研究 | zh_TW |
dc.title | Phylogenetic relationships and reproductive biology of
Mussaenda species in Taiwan | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 謝長富(chang-Fu Hsieh),楊宗愈(Tsung-Yu Aleck Yang) | |
dc.subject.keyword | 玉葉金花,台北玉葉金花,生殖生物學, | zh_TW |
dc.subject.keyword | Mussaenda parviflora,M. taihokuensis,Reproductive biology, | en |
dc.relation.page | 101 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-07-31 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生態學與演化生物學研究所 | zh_TW |
顯示於系所單位: | 生態學與演化生物學研究所 |
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