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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28003
標題: | 製備具微米構形之聚電解質多層膜及探討細胞貼附的影響 Fabrication of micropatterned polyelectrolyte multilayers for controlling cell adhesion |
作者: | Tsung-Yao Chang 張琮瑤 |
指導教授: | 蔡偉博(Wei-Bor Tsai) |
關鍵字: | 細胞貼附,聚電解質多層膜,層接層,微構形,聚丙烯酸,聚丙烯醯胺, cell adhesion,polyelectrolyte multilayers,layer by layer,micropattern,polyacrylic acid,polyacrylamide, |
出版年 : | 2007 |
學位: | 碩士 |
摘要: | 具微米構形之聚電解質多層膜能控制表面地形尺寸至分子級,非常適合體外培養細胞及生醫材料的研究上。
聚丙烯酸(polyacrylic acid,PAA)與聚丙烯醯胺(poly acrylamide,PAAm)在低酸鹼值時,可由氫鍵形成聚電解質多層膜,而且可被鹼性水洗去。配合上疊氮化物(azide)可在紫外光照射下與聚合物交聯的特性,來製備具微米構形之聚電解質多層膜。 首先將4-疊氮苯胺鹽酸鹽(4-azidoaniline hydrochloride)與聚丙烯酸接枝形成PAAz。接著,在載玻片上製作PAA/PAAm多層膜至4個雙層,再吸附PAAz/PAAm多層膜1個雙層後,繼續堆疊PAA/PAAm。將不同雙層數的此多層膜在等尺寸線寬、間距,週期分別為20 μm、50 μm之平行直線光罩下照射紫外光後,用pH 10.0的二次水洗去未交聯的區域,就形成具微米構形之聚電解質多層膜。 進行細胞實驗前,先在此表面上吸附一層聚離胺酸(poly-L-lysine,PLL),我們發現MG63、L929細胞會選擇在避光的區域貼附、排列,PC12細胞則是照光、避光區域皆會排列,但其突觸會沿著平行線的方向延伸。本研究結果觀察到細胞在週期50 μm的微米構形多層膜表面上排列情況較週期20 μm佳。 本研究成功地利用光刻法製備可調控細胞貼附的具微米構形之聚電解質多層膜。 The topography of micropatterned polyelectrolyte multilayers can be controlled in molecule scale and is extremely versatile and useful for in vitro studies of cell behavior or biomedical devices. Polyacrylamide (PAAm) and poly(acrylic acid) (PAA) can be assembled to form polyelectrolyte multilayers via hydrogen-bonding interactions at low pH. The PAA/PAAm films can be rinsed away with alkaline water. Azides, a photo-reactive molecule, can crosslink neighbor hydrocarbon chains under UV irradiation. First, 4-azidoaniline hydrochloride was grafted on PAA to form PAAz. Then, PAA/PAAm films were fabricated on glass slide for 4 bilayers, followed by deposition of one PAAz/PAAm bilayer before further build-up of PAA/PAAm films. Micropatterned polyelectrolyte multilayers with different numbers of bilayers were fabricated by UV exposure of the multilayers films through photomasks with 20 μm or 50 μm periods of parallel lanes. Poly-L-lysine was adsorbed on micropatterned films before in vitro cell experiments. We found MG63 and L929 cells adhered to ditch region and regularly arranged along the direction of ditch. On the other hand, PC12 cells extended neurites parallel to the striped pattern, but adhered on both ditch and ridge regions. Cell alignment on the micropatterned films with 50 μm periods was better than 20μm periods. These works successfully developed a technique utilizing polyelectrolyte multilayers and photolithography technology to prepare micropatterned substrata with controlled surface chemistry for modulation of cell adhesion. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/28003 |
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顯示於系所單位: | 化學工程學系 |
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