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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 沈弘俊(Horn-Jiunn Sheen) | |
dc.contributor.author | Jen-Hao Hsu | en |
dc.contributor.author | 許仁豪 | zh_TW |
dc.date.accessioned | 2021-06-12T18:27:41Z | - |
dc.date.available | 2008-08-28 | |
dc.date.copyright | 2007-08-28 | |
dc.date.issued | 2007 | |
dc.date.submitted | 2007-08-08 | |
dc.identifier.citation | Y Huang and R Pethlg, “Electrode design for negative Dielectrophoresis”, Measurement Science and Technology, Vol. 2, pp. 1142-1146, 1991.
Chunxiang Xu,Yan Wang, Ming Cao and Zuhong Lu, “Dielectrophoresis of human red cells in microchips”, Electrophoresis, Vol. 20, pp. 1829-1831, 1999. Hywel Morgan, Michael P. Hughes, and Nicolas G. Green, “Separation of Submicron Bioparticles by Dielectrophoresis”, Biophysical Journal, Vol. 77, pp.516–525, 1999. Peter Gascoyne, Chulabhorn Mahidol, Mathuros Ruchirawat, Jutamaad Satayavivad, Piyajit Watcharasit and Frederick F. Becker, “Microsample preparation by dielectrophoresis: isolation of Malaria”, Lab on a Chip, Vol. 2, pp. 70-75, 2002. Nikolai Markarian, Mike Yeksel, Boris Khusid, and Kenneth Farmer, “Limitations on the scale of an electrode array for trapping particles in microfluidics by positive dielectrophoresis”, Applied Physics Letters, Vol. 82, pp. 4839-4841, 2003. Manfried Dürr, Jorg Kentsch, Torsten Muller, Thomas Schnelle, Martin Stelzle, “Microdevices for manipulation and accumulation of micro- and nanoparticles by dielectrophoresis”, Electrophoresis, Vol. 24, pp. 722–731, 2003. Sreeja B. Asokan, L. Jawerth, R. Lloyd Carroll, R. E. Cheney, S. Washburn, and R. Superfine, “Two-Dimensional Manipulation and Orientation of Actin-Myosin Systems with Dielectrophoresis”, Nano Letters, Vol. 3, No. 4, pp. 431-437, 2003. Janko Auerswald, Helmut F. Knapp, “Quantitative assessment of dielectrophoresis as a micro fluidic retention and separation technique for beads and human blood erythrocytes”, Microelectronic Engineering, Vol. 68, pp. 879–886, 2003. Neftali Flores-Rodriguez and Gerard H Markx, “Improved levitation and trapping of particles by negative dielectrophoresis by the addition of amphoteric molecules, Journal of Physics D: Applied Physics, Vol. 37, pp. 353–361, 2004. Vasile I. Furdui and D. Jed Harrison, “Immunomagnetic T cell capture from blood for PCR analysis using microfluidic systems”, Lab on a Chip, Vol. 4, pp. 614–618, 2004. Il Doh, Young-Ho Cho, “A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process”, Sensors and Actuators A , Vol. 121, pp. 59–65, 2005. Hao Zhou, Lee R. White, Robert D. Tilton, “Lateral separation of colloids or cells by dielectrophoresis augmented by AC electroosmosis”, Journal of Colloid and Interface Science, Vol. 285, pp. 179–191, 2005. Jeroen H. Nieuwenhuis, Artur Jachimowicz, Peter Svasek, and Michiel J. Vellekoop, Senior Member, “Optimization of Microfluidic Particle Sorters Based on Dielectrophoresis”, IEEE Sensors Journal, Vol. 5, NO. 5, pp. 810-816, 2005. Philip J. Costanzo, Enzhu Liang, Timothy E. Patten, Scott D. Collins and Rosemary L. Smith, “Biomolecule detection via target mediated nanoparticle aggregation and dielectrophoretic impedance measurement”, Lab on a Chip, Vol. 5, pp. 606–610, 2005. Janko Auerswald, David Widmer, Nico F. de Rooij, Andre Sigrist, Thomas Staubli, Thomas Stockli, Helmut F. Knapp, “Fast immobilization of probe beads by dielectrophoresis-controlled adhesion in a versatile microfluidic platform for affinity assay”, Electrophoresis, Vol. 26, pp. 3697–3705, 2005. Kwang-Seok Yun, Dohoon Lee, Hak-Sung Kim and Euisik Yoon, “A microfluidic chip for measurement of biomolecules using a microbead-based quantum dot fluorescence assay”, Instittute of Physics Publishing, Measurement Science and Technology, Vol. 17, pp.3178–3183, 2006. Liju Yang, Padmapriya P. Banada, Mohammad R. Chatni, Kwan Seop Lim, Arun K. Bhunia, Michael Ladischde and Rashid Bashir, “A multifunctional micro-fluidic system for dielectrophoretic concentration coupled with immuno-capture of low numbers of Listeria monocytogenes”, Lab on a Chip, Vol. 6, pp. 896–905, 2006. Kwan Hyoung Kang, Yuejun Kang, Xiangchun Xuan ,Dongqing Li, “Continuous separation of microparticles by size with Direct current-dielectrophoresis”, Electrophoresis, Vol. 27, pp. 694–702, 2006. David Holmes, Hywel Morgan, Nicolas G. Green, “High throughput particle analysis: Combining dielectrophoretic particle focussing with confocal optical detection”, Biosensors and Bioelectronics, Vol. 21, pp. 1621–1630, 2006. 劉振邦, “粒子操控之微混合器晶片開發”, 國立台灣大學應用力學所碩士論文, 2002. 黃志宇, “粒子操控術於分離脆性紅血球之應用”, 國立台灣大學應用力學所碩士論文, 2006. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27915 | - |
dc.description.abstract | 本研究利用介電泳原理搭配微機電製程方式,成功製作出測試人體血液中分離出γ球蛋白的分離晶片,整個流程僅需兩道光罩(微電極陣列晶片與微流道母模各一)即可完成晶片製作。本實驗採用的γ球蛋白與 Anti-Human IgG皆由瑞博公司所購買,再與購買自MERCK公司的粒子,粒子上面有一層羧基群(carboxyl group),使Anti-Human IgG鍵結聚苯乙烯粒子表面,再讓Anti-Human IgG與γ球蛋白中的IgG作反應,成為一複合體粒子。吾人將紅血球與此複合體粒子作混合,在吾人所製作的生物晶片中,成功做出分離現象,操作電壓為10Vpp、頻率2MHz。由於此生物晶片可以於低電壓下迅速分離紅血球與人體血清蛋白質,所以此微分離晶片之開發對於人體上的應用,將有極大的幫助。此晶片在生物實驗方面,將可以幫助在使用免疫擔體沉澱法時,只需應用上少量的抗體與抗原,減少醫療耗費即可做出蛋白質的分析。 | zh_TW |
dc.description.abstract | This study presents a new bio-chip which can separate the gamma globulin from the human serum based on dielectrophoresis forces. MEMS fabrication process was used to produce the bio-chip and only two photomasks were required in the experiment. Anti-human IgG was bound on the polymer beads surface with carboxyl group to be complex beads. Gamma globulin was then mixed with the complex beads heterogeneously. The mixtures which contained both bio-particles and RBCs were injected into the bio-chip to test the separation efficiency. The driving source, 10Vpp and 2MHz, was used to separate RBCs and bio-particles. The current of this chip was less than human perception current so that it was safe for biologic tests. Furthermore, Immunoprecipitation showed that small amount of samples were needed for this chip to detect the antibody or antigen easily. | en |
dc.description.provenance | Made available in DSpace on 2021-06-12T18:27:41Z (GMT). No. of bitstreams: 1 ntu-96-R94543061-1.pdf: 3076201 bytes, checksum: b5c1671641a4666636d9179e21ed3c85 (MD5) Previous issue date: 2007 | en |
dc.description.tableofcontents | 目錄..................................................i
圖目錄................................................v 表目錄................................................viii 符號說明..............................................ix 目錄 第一章 研究背景 1-1研究背景.............................1 1-1-1人體血球與蛋白質.................................1 1-1-2微機電系統技術(MEMS).............................3 1-1-3介電泳力(dielectrophoresis)的應用................4 1-2 文獻回顧..........................................5 1-3 研究動機與目的....................................9 1-3-1研究動機.........................................9 1-3-2研究目的.........................................11 第二章 粒子操控原理 2-1 介電泳原理........................................13 2-1-1介電泳力的形成方式...............................13 2-1-2誘導偶極矩(Induce dipole moment).................13 2-1-3旅波介電泳(Traveling Wave Dielectrophoresis).....20 2-2其他影響力之探討...................................22 2-2-1布朗運動.........................................22 2-1-2電泳力...........................................23 第三章 實驗方法與設備 3-1 粒子操控晶片設計..................................24 3-1-1 電極金屬材料....................................25 3-1-2 電極晶片基板選擇................................26 3-1-3 電極排列設計....................................26 3-1-4 光罩選擇與製作..................................27 3-1-5 金屬薄膜沉積技術................................27 3-2 MEMS製程技術......................................28 3-2-1 基材清潔(Clean).................................29 3-2-2 光阻塗佈(Coating)...............................29 3-2-3 軟烤(Soft bake).................................30 3-2-4 曝光(Exposure)..................................30 3-2-5 顯影(Development)...............................30 3-2-6 硬烤(Hard bake).................................31 3-2-7 運用MEMS技術製造電極晶片........................31 3-2-7-1 基板清洗......................................31 3-2-7-2 電極蒸鍍......................................32 3-2-7-3 電極晶片微影與蝕刻製程........................33 3-2-8 運用MEMS技術製造微流道..........................35 3-2-8-1 SU-8微流道母模製作............................35 3-2-8-2 PDMS微流道製作................................37 3-3 元件接合與外部連結方式............................38 3-4 實驗設備..........................................39 3-5 生物粒子的選取....................................41 3-5-1 紅血球的製備....................................42 3-5-2 免疫球蛋白鍵結擔體的製備........................42 3-6 溶液與溶液導電度的選取............................43 3-7 分離晶片的設計....................................45 第四章 實驗結果與討論 4-1 紅血球介電特性測試................................46 4-2 奈米生物粒子之分離................................48 4-2-1 生物粒子種類....................................48 4-2-2 如何於同一畫面呈現兩種螢光粒子..................52 4-2-3 螢光粒子分離結果與討論..........................52 4-2-4 分離奈米粒子注意事項............................53 4-3 研究晶片分離結果與討論............................54 4-3-1 研究晶片注意事項................................56 4-4 SDS膠體電泳.......................................57 第五章 結論及未來展望 5-1 結論..............................................60 5-2 未來展望..........................................62 參考文獻..............................................64 圖目錄 圖1.1蛋白質於電泳膠片上之示意圖.......................67 圖1.2 黑色部份為polynomial電極,亮色部份為TMV病毒,呈現正 介電泳現象。Morgan et al. 1999........................67 圖1.3 黑色部份為polynomial電極,亮色部份為HSV-1病毒,呈現 負介電泳現象。Morgan et al. 1999......................68 圖1.4 黑色部份為castellated電極,紅色部份為鍵結上IgG的粒子, 綠色部分粒子表面無化學處理,成功分離開兩種粒子。 Morgan et al. 1999....................................68 圖1.5 分離不同大小粒子的晶片示意圖。Dürr et al. 2003 .69 圖1.6 單顆粒子在流道中鍵結Human IgG與Anti-Human IgG的示意圖。Yun et al. 2006...................................69 圖1.7 分析注入之螢光Anti-Human IgG的濃度之不同,所造成的單 顆粒子螢光強度之不同。Yun et al. 2006.................70 圖1.8 注入含有Listeria monocytogenes的流體,利用pDEP力使 Listeria monocytogenes與Anti-Listeria monocytogenes產生 鍵結的螢光分析。Yang et al. 2006......................70 圖1.9 免疫擔體沉澱法示意圖............................71 圖2.1 誘導偶極矩與電場關係圖..........................71 圖2.2 粒子受正負介電泳之移動情形......................72 圖2.3 雙層球殼結構....................................72 圖2.4 微流道中旅波式介電泳電極示意圖(立體圖)...........73 圖2.5 微流道中旅波式介電泳電極示意圖(側視圖)...........73 圖2.6 粒子在旅波式電場中之受力行為示意圖,Morgan (1997).74 圖3.1 測試電極晶片設計圖(平行電極)......................74 圖3.2 晶片製作電極部分流程圖............................75 圖3.3 研究晶片之電極部分................................75 圖3.4 微流道製作流程圖..................................76 圖3.5 SU-8微流道母模示意圖..............................76 圖3.6 PDMS微流道與電極晶片接合封裝流程圖................77 圖3.7 粒子分離晶片實體圖................................77 圖3.8 PDMS接合設備圖....................................78 圖3.9 實驗整套量測系統配置..............................78 圖4.1 紅血球滲透壓試驗圖................................79 圖4.2 晶片設計示意圖....................................79 圖4.3 紅血球負介電泳現象................................80 圖4.4 鍵結抗體之聚苯乙烯粒子示意圖.......................81 圖4.5 Type A~G正負介電泳現象示意圖.......................81 圖4.6 Type A粒子之正介電泳現象...........................82 圖4.7 Type A粒子之負介電泳現象...........................83 圖4.8 Type G粒子受電場後之移動現象.......................84 圖4.9 濾光鏡波長圖.......................................85 圖4.10 直徑300nm,螢光粒子波長圖.........................86 圖4.11 Type G與Type A之分離..............................87 圖4.12 Type G與Type B之分離..............................88 圖4.13 Type C與Type A之分離..............................89 圖4.14 紅血球於分離晶片之測試............................90 圖4.15 紅血球與Type G之分離..............................91 圖4.16 SDS膠體電泳實驗結果圖.............................92 表目錄 表1.1 人體血清中蛋白質的比例.............................93 表3.1 石英光罩、玻璃光罩及膠片光罩之特性比較.............93 表3.2 SU-8 1060製程參數..................................94 | |
dc.language.iso | zh-TW | |
dc.title | 微奈米生物粒子分離之介電泳晶片開發 | zh_TW |
dc.title | Development of a Microchip to Separate Micro/Nano
Bio-particles by Dielectrophoresis | en |
dc.type | Thesis | |
dc.date.schoolyear | 95-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 吳光鐘(Kuang-Chong Wu),林世明(Shi-Ming Lin),李雨(U Lei) | |
dc.subject.keyword | 介電泳,γ球蛋白,Anti-Human IgG, | zh_TW |
dc.subject.keyword | Dielectrophoresis,Gamma globulin,Anti-Human IgG, | en |
dc.relation.page | 94 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2007-08-09 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 應用力學研究所 | zh_TW |
顯示於系所單位: | 應用力學研究所 |
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