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標題: | 利用微粒子追蹤測速儀量測C反應蛋白之布朗運動及其反應檢測 Detection of C Reactive Protein Based on Measurement of Brownian Motion by Micro Particle Tracking Velocimetry |
作者: | Yu-Jui Fan 范育睿 |
指導教授: | 沈弘俊(Sheen, Horn-Jiunn) |
關鍵字: | 布朗運動,微粒子追蹤測速儀,生物感測技術,C反應蛋白, Brownian motion,micro Particle Tracking Velocimetry,bio-sensor technique,C-reactive protein, |
出版年 : | 2007 |
學位: | 碩士 |
摘要: | 在本篇研究中,藉由量測奈米粒子的布朗運動關係來開發一個新的CRP感測技術。將anti-CRP修飾在帶有螢光的奈米粒子上,anti-CRP會因與CRP有專一性而吸附上CRP,奈米粒子的布朗運動速度則會因為粒子的變大而減緩。此種感測技術在於疾病或是病毒上的感測非常有用,使用上也非常簡單且價格低廉,而且是非常容易與任何的微流系統做晶片上的整合。布朗運動的量測是以為粒子追蹤測速儀作為量測工具。實驗結果中發現,當粒子表面吸附上抗體,粒子會因為CRP的吸附,粒徑從300nm 增加到400nm,而粒子布朗運動速度下降。這意味著布朗運動的速度下降率與粒徑有關的。布朗運動速度下降率與CRP濃度有關;濃度越高下降的速率會越快。布朗運動最後平衡的速度也與濃度呈正相關。而感測的過程不會耗費太多時間,容易做到real-time感測以及整合在攜帶式生物晶片上。未來,可以使用全反射螢光顯微鏡配合微粒子追蹤測速儀用於量測布朗運動,對於較小的粒子可以獲得更好的結果。 In this research, a new CRP sensing technique has been successfully developed by detecting Brownian motions of nano polymer beads. The anti-CRP is coated on the surface of the fluorescent nano-beads to conjugate the CRP in the buffer solution. The Brownian motion velocity becomes slower when the bead size increases during the reaction process. This sensing technique is very useful for the detection of some diseases or virus. In addition, low cost and easy operation enable this technique to be easily integrated in some microfludic devices. In order to obtain the characteristics of particle Brownain motion in the static liquid, a measurement by using micro-particle-tracking-velocimetry (μ-PTV) is employed in our work. The results showed that the diameter of the bead increased from 300nm to 400nm when the CRP was conjugated on the bead surface. The reaction process indicated that the decreasing rates of Brownian velocities depended on the CRP concentration. The Brownian velocities decreased rapidly for high concentration of CRP like 100g/ml.The lower Brownian velocity was obtained for higher CRP like 100g/ml. the |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27792 |
全文授權: | 有償授權 |
顯示於系所單位: | 應用力學研究所 |
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