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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 李篤中(Duu-Jong Lee) | |
dc.contributor.author | Chin-Yu Lee | en |
dc.contributor.author | 李慶昱 | zh_TW |
dc.date.accessioned | 2021-06-12T18:04:44Z | - |
dc.date.available | 2012-09-01 | |
dc.date.copyright | 2011-09-21 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-08 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27430 | - |
dc.description.abstract | 微生物燃料電池(Microbial fuel cell,MFC),可藉由微生物將有機廢水中的化學能直接轉變為電能,為一新興的廢水處理單元。本論文分別使用Pseudomonas sp. C27 及硫酸鹽還原菌 (Sulfate-reducing bacteria , SRB) 為微生物燃料電池陽極生物膜之菌源,探討MFC產電特性及廢水處理效果。
結果顯示,以Pseudomonas sp. C27啟動之MFC系統在處理含硫化物廢水時有較高輸出電壓 (140 mV) 及電流密度值 (240 mA/m2),且當初始硫離子濃度為125.2 ppm時,硫離子去除率最高達98.4%,但長期運行下因受外來雜菌侵入導致產電效能下降。以SRB啟動之MFC在陽極培養液為乳酸和硫酸鹽時,具有較高輸出電壓 (308 mV) 和電流密度值 (513 mA/m2) ,最大功率密度為255 mW/m2,且在不同條件下,皆可維持80%以上硫酸鹽去除率。另外,SRB與C27複合電極型之生物燃料電池除了可增加總產電量,提升產電效率,亦可改善硫離子累積之缺點。 | zh_TW |
dc.description.abstract | Microbial Fuel Cell (MFC) is a promising new technology which combines wastewater treatment with energy production. MFC uses bacteria as catalysts to convert chemical energy from organic matters in wastewater into electrical power. In this thesis, two different types of bacteria were used for the inoculation of MFCs’ anodes: Pseudomonas sp. C27, and Sulfate-reducing bacteria, SRB. The influence of different bacteria used on the MFC anode on the electricity generation characteristics and wastewater treatment performance of MFC were evaluated.
The Pseudomonas sp. C27 MFC system produces its maximum voltage and current density at 140 mV and 240 mA/m2, when fed with sulfide only substrates; the sodium sulfide removal efficiency can reach as high as 98.4%. However, it is difficult to maintain a pure bacteria culture system under long operating time due to intrusion of other bacteria. The current generation of Pseudomonas sp. C27 MFC system drops over time. MFC systems inoculated with SRB and fed with lactate and sulfate has the maximum voltage at 308V, maximum current density at 513 mA/m2 and maximum power density at 254.84 mW/m2. Also, under different substrate conditions, sulfate removal efficiency can reach above 80%. Finally, the MFC system with both SRB and C27 can produce high voltage and electricity generation efficiency. | en |
dc.description.provenance | Made available in DSpace on 2021-06-12T18:04:44Z (GMT). No. of bitstreams: 1 ntu-100-R98524062-1.pdf: 13260912 bytes, checksum: e90d20b0619a2517ec1db4fd2a91f5de (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 誌謝 I
摘要 II Abstract III 目錄 IV 圖目錄 VIII 表目錄 XII 第一章 前言 1 第二章 文獻回顧 2 2-1 全球能源問題及廢水處理 2 2-2 微生物燃料電池原理 2 2-3 微生物燃料電池類型 3 2-3-1 依微生物燃料電池的外型分類 3 2-3-1-1 單室MFC (Single-chambered microbial fuel cell) 4 2-3-1-2 雙室MFC (Two-chambered microbial fuel cell) 4 2-3-1-3 沉積物 MFC (Sediment MFC,SMFC) 5 2-3-2 依微生物燃料電池電子傳遞方式分類 5 2-3-2-1 電子中介體MFC 5 2-3-2-2 無電子中介體MFC 5 2-3-3 依微生物燃料電池的微生物類型分類 6 2-4微生物燃料電池研究現況 7 第三章 實驗材料與方法 8 3-1實驗材料 8 3-1-1 微生物接種 8 3-1-2 接種培養液組成 8 3-1-3 MFC之建構 10 3-1-4 電極製作 11 3-1-5 微生物電極的培養 11 3-1-6 離子交換膜 11 3-1-7 微生物厭氧液體 12 3-1-8 陰極槽反應液 12 3-2實驗方法 12 3-2-1 化學需氧量 (COD) 測定 12 3-2-2 pH測定 12 3-2-3 硫化物測定 13 3-2-3-1 方法 13 3-2-3-2 試劑 13 3-2-3-3步驟 13 3-2-3-4計算結果處理 14 3-2-4 質量平衡計算單質硫產率 14 3-2-5 陰離子濃度分析 14 3-2-6 掃描式電子顯微鏡 (Scanning electron microscope,SEM) 18 3-2-7 雷射掃描共軛焦顯微鏡 (Confocal laser scanning microscope,CLSM) 18 3-2-8 變性梯度凝膠電泳 (Denaturing gradient gel electrophoresis , DGGE) 19 3-2-8-1 微生物DNA 萃取 19 3-2-8-2 聚合酶連鎖反應 (Polymerase chain reaction, PCR) 19 3-2-8-3 以變性梯度凝膠電泳法分析菌相 20 3-2-8-3-1 細菌之變性梯度膠體電泳 20 3-2-8-3-2 核酸染色及影像檢視 21 3-2-8-3-3 膠體中DNA之純化回收 21 3-2-8-3-4 核酸定序 21 3-2-9 電化學分析 21 3-2-9-1 電壓記錄 21 3-2-9-2 電流密度 22 3-2-9-3 線性掃描伏安法 (LSV) 22 3-2-9-4 極化曲線 (Polarization curve) 和功率密度曲線 (Power density curve) 22 3-2-9-5 電位分佈 23 3-2-9-6 庫倫效率 (Coulombic efficiency, CE) 23 3-3 實驗設計 24 3-3-1 以Pseudomonas sp. C27純菌啟動之雙槽式生物燃料電池 24 3-3-1-1 不同培養液下之產電特性 24 3-3-1-2 不同硫化鈉濃度下之產電特性 24 3-3-1-3 不同碳源下之產電特性 25 3-3-2以硫酸還原菌群啟動之雙槽式微生物燃料電池 25 3-3-2-1不同培養液下之生物產電特性 25 3-3-3 複合式之微生物燃料電池 26 第四章 結果與討論 28 4-1 以Pseudomonas sp. C27純菌啟動之雙槽式微生物燃料電池 28 4-1-1 不同培養液下之產電特性 28 4-1-1-1 產電特性 28 4-1-1-2 電化學分析 31 4-1-1-3 代謝產物 39 4-1-1-4 陽極生物膜穩定性 43 4-1-1-5 陽極生物膜型態 44 4-1-1-5-1 SEM分析 44 4-1-1-5-2 CLSM分析 49 4-1-2 長期操作下陽極生物膜之微生物群落 50 4-1-2-1不同硫化鈉濃度下之產電特性 52 4-1-2-1-1 空白實驗 52 4-1-2-1-2 產電特性 53 4-1-2-1-3 電化學特性 55 4-1-2-1-4 硫化物濃度之可逆性 56 4-1-2-1-5 不同陽極材料下之產電特性 57 4-1-2-2不同碳源下之產電特性 60 4-2 以硫酸還原菌群啟動之雙槽式微生物燃料電池 62 4-2-1 不同培養液下之產電特性 62 4-2-1-1 產電特性 62 4-2-1-2 電化學特性 65 4-2-1-3 代謝產物 68 4-2-2 陽極生物膜型態 73 4-2-2-1 SEM分析 73 4-2-2-2 CLSM分析 76 4-2-3 陽極生物膜之微生物群落 78 4-3 提升產電效率及改善硫離子濃度之累積 82 4-3-1 反應器設計 83 4-3-2 產電特性 83 4-3-3 電化學特性 86 4-3-4 代謝產物 90 第五章 結論 96 參考文獻 97 | |
dc.language.iso | zh-TW | |
dc.title | 微生物燃料電池處理含硫廢水及產電特性 | zh_TW |
dc.title | Use of Microbial Fuel Cell for Treating Sulfur-Containing Wastewater and Electricity Production | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃志彬,張嘉修,劉志成,朱曉萍 | |
dc.subject.keyword | 微生物燃料電池,含硫廢水,硫酸鹽還原菌, | zh_TW |
dc.subject.keyword | Microbial Fuel Cell,Sulfur-Containing Wastewater,Sulfate-reducing bacteria, | en |
dc.relation.page | 102 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2011-08-09 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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