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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 董成淵 | |
dc.contributor.author | Po-Shou Chang | en |
dc.contributor.author | 張栢壽 | zh_TW |
dc.date.accessioned | 2021-06-12T18:03:11Z | - |
dc.date.available | 2010-01-30 | |
dc.date.copyright | 2008-01-30 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-01-22 | |
dc.identifier.citation | 1. C. Y. Dong, K. K, P. S, “Characterizing point spread functions of two-photon fluorescence microscopy in turbid”, Journal of Biophysical Journal (82), 3300-3342 (2002)
2. Peter T. C. So, Chen Y. Dong, Barry R. Master, and Keith M. Berland, “Two-photon excitation fluorescence microscopy”, Annual Review of Biomedical Engineering 2, 399-429 (2000) 3. R. W. Boyd, “Nonlinear optics”, second edition, Academic Press, Boston, MA, USA (2003) 4. R. Shankar, “Principles of Quantum Mechanics”, (Plenum, 1994) 5. http://www.siumed.edu/~dking2/index.htm 6. http://www.hcvets.com/index.html 7. http://www.courseweb.uottawa.ca/medicine-histology/English/Gastrointestinal/Liver.htm 8. Christian R. H. Raetz and Chris Whitfield “Lipopolysaccharide endotoxins”, Annual Review of Biochemistry (71), 635-700 (2002) 9. Christian R. H. Raetz, C. Michael Reynolds, M. Stephen Trent, and Russel E. Bishop, “Lipid A Modification Systems in Gram-Negative Bacteria”, Annual Review of Biochemistry (76), 295-329 (2007) 10. Hiroyuki Katagiri, Yoshiya Ito, Ken-ichiro Ishii, Izumi Hayashi, Makoto Suematsu, Shohei Yamashina, Takahiko Murata, Shuh Narumiya, Akira Kakita, and Masataka Majima, “Role of thromboxane derived from COX-1 and-2 in hepatic microcirculatory dysfunction during endotoxemia in mice”, Hepatology, (39), 139-150 (2004) 11. http://www.sigmaaldrich.com/Area_of_Interest/The_Americas/United_States.html 12. Vito Racanelli and Barbara Rehermann, “The Liver as an Immunoiogical Organ” Hepatology, (43), S54-S62 (2006) 13. Yong-Han Paik, Robert F. Schwabe, Ramon Bataller, Maria P. Russo, Christian Jobin and David A. Brenner, “Toll-Like receptor 4 mediates inflammatory signaling by bacterial lipopolysaccharide in human hepatic stellate cells”, Hepatology, (37), 1043-1055 (2003) 14. Grace L. Su, Ke Qin Gong, ming Hui Fan, William M. Kelley, Jason Hsieh, Jian Min Sun, Mark R. Hemmila, Saman Arbabi, Daniel G. Remick, and Stewart C. Wang, “Lipopolysaccharide-Binding Protein Modulates Acetaminophen-Induced Liver Injury in MIce” Hepatology, (41), 187-195 (2005) 15. Marike van Oosten, Erika van de Bilt, Helga E. De Vries, Theo J. C. Van Berkel, and Johan Kuiper, “Vascular Adhesion Molecule-1 and Intercullar Adhesion Molecule-1 Expression on Rat Liver Cells after Lipopolysaccharide Administration In Vivo” Hepatology, (22), 1538-1546 (1995) 16. Steven M. Kerfoot and Paul Kubes, “Local coordination verses systemic disregulation: complexities in leukocyte recruitment revealed by local and systemic activation of TLR4 in vivo” Journal of Leukocyte Biology, (77), 862-867 (2005) 17. Grace L. Su, “Lipopolysaccharide in liver injury: molecular mechanisms of Kuppfer cell activation”, American Journal of Physiology-Gastrointestinal and Liver Physiology, (283), G256-G265, (2002) 18. Mark Lehnert, Tetsuya Uehara, Blair U. Bradford, Henrik Lind, Zhi Zhong, David A. Brenner, Ingo Marzi, and John J. Lemasters, “Lipopolysaccharide-binding protein modulates hepatic damage and the inflammatory response after hemorrhagic shock and resuscitation” American Journal of Physiology-Gastrointestinal and Liver Physiology, (291), G456-G463, (2006) 19. P. Sheth, N. Delos Santos, A. Seth, N. F. Larusso, and R. K. Rao, “Lipopolysaccharide disrupts tught junctions in cholangiocyte monolayers by a c-Src-, TLR4-, and LBP-dependent mechanism” American Journal of Physiology-Gastrointestinal and Liver Physiology, (293), G308-G318, (2007) 20. C. Eipel, R.Bordel, R. M. Nickels, M. D. Menger, and B. Vollmar, “Impact of leukocytes and platelets in mediaitng hepatocyte apoptosis in a rat model of systemic endotoxemia” American Journal of Physiology-Gastrointestinal and Liver Physiology, (286), G769-G776, (2004) | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27384 | - |
dc.description.abstract | 台灣有四百萬人為B型肝炎的帶原者,而慢性肝炎甚至肝癌都有可能導致致命的傷害,根據行政院衛生署統計,癌症為十大死因之首,而肝癌又排名在男性癌症死因的第一名,慢性B肝或慢性C肝亦是導致肝癌或其他肝臟病變的重要原因,遺憾的是肝臟的問題在早期階段患者往往感受不到任何的病痛而延誤就醫的時間,因此尋求一個快速有效且對患者產生最低疼痛的診斷方式,及一種觀察肝臟活體代謝的研究模式,多光子顯微術也許將成為最佳的選擇。另外,傳統上為獲得生物組織的生理學資料,往往選擇有高解析度影像的切片技術,但其過程須以化學方式處理固定卻導致無法取得活體內的即時影像,且僅能獲得二維的資料,而本實驗室發展之肝臟視窗平台成為上述諸多限制的一大突破,本研究即以雙光子顯微術結合肝臟視窗平台,即以非侵入式方式觀察內毒素(脂多醣類-LPS)誘發老鼠肝臟發炎的即時反應。在取得的影像中我們觀察到Rhodamine-6G染色之白血球在免疫系統反應中首先聚集於肝臟,並開始滾動及附著於血管壁,接著穿透血管及細胞壁進入發炎組織,我們也發現不僅發炎後不同的肝細胞6-CFDA代謝速率之不同,即使未發炎老鼠的不同肝細胞亦有不同代謝速率。我們更期望我們的發展技術對未來肝臟甚至其他活體器官的觀察研究有所啟發及貢獻。 | zh_TW |
dc.description.abstract | The parenchyma of liver has a highly organized and densely crowded sinusoidal system. They are tiny capillary-like vessels, therefore leukocytes and other blood cells must traverse through these vessels in contact with the endothelial cells flanking the sinusoids. By the use of intravital two-photon microscopy and hepatic imaging chamber we can study the response in vivo after administration of lipopolysaccharide (LPS), which is commonly used to model inflammatory mechanism. Whereas it is a critical and apparent process of the inflammatory response that the leukocytes recruitment at the inflamed tissues, these leukocytes roll along the vascular wall until they encounter stimulus produced by the endothelium cells and macrophages. The fluorescence micrographs of the hepatic microcirculation show that leukocytes accumulate within the sinusoids, then transmigrate across endothelial layer and into the inflammatory hepatocytes. We can also observe the metabolism of 6-CFDA within hepatocytes and the subsequent excretion in bile canaliculi. | en |
dc.description.provenance | Made available in DSpace on 2021-06-12T18:03:11Z (GMT). No. of bitstreams: 1 ntu-97-P94222005-1.pdf: 1419364 bytes, checksum: f0ed238014b742128a35aba6bb7265b8 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | Contents 1
Figure Contents 3 Abstract 5 摘要 6 Chapter 1 Introduction 7 Chapter 2 Basic Principles 10 2.1 Second Harmonic Generation 10 2.2 Two-Photon Fluorescence 13 2.3 Optical Properties of Two-Photon Microscopy 19 Chapter 3 Histology of Liver 21 3.1 Liver Function and Structure 21 3.2 Intrahepatic Vascular and Biliary System 27 3.3 Hepatic Elements 30 Chapter 4 Experimental Material, Setup and Method 35 4.1 Drug 35 4.2 Two-photon Microscope Setup 36 4.3 Experimental Materials 38 4.4 Installation of Hepatic Image Chamber 40 Chapter 5 Results and Discussion 42 5.1 Visualization of Hepatic Parenchyma 42 5.2 Visualization of Hepatobiliary Excretory Function 44 5.3 Leukocyte Recruitment in Response to Administration of LPS 48 Chapter 6 Conclusions 56 References 58 | |
dc.language.iso | en | |
dc.title | 利用非侵入式多光子顯微術觀察脂多醣類(LPS)誘發老鼠之肝臟發炎 | zh_TW |
dc.title | Using Intravital Multi-photon Fluorescence Microscopy in the Investigation of Mouse Liver Inflammation Induced by Lipopolysaccharide(LPS) | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李宣書,曹培熙,張顏輝 | |
dc.subject.keyword | 雙光子顯微術,脂多醣類,白血球, | zh_TW |
dc.subject.keyword | two-photon microscopy,lipopolysaccharide (LPS),inflammation,leukocyte, | en |
dc.relation.page | 61 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2008-01-23 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 物理研究所 | zh_TW |
顯示於系所單位: | 物理學系 |
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