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標題: | 阿拉伯芥ethylene response factor1(AtERF1)於非生物逆境下的功能性分析 Functional characterization of ethylene response factor1 (AtERF1) in response to abiotic stresses in Arabidopsis |
作者: | Po-Ming Liao 廖柏茗 |
指導教授: | 林讚標(Tsan-Piao Lin) |
關鍵字: | 阿拉伯芥,非生物逆境, ERF1,abiotic stress,Arabidopsis, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 非生物逆境,包含高鹽以及乾旱常會影響農作物產量,同時也會影響植株內大量的基因表現。而植物會於轉錄層面進行基因的調控,促使下游基因使之活化或抑制以因應環境逆境。本研究中透過乾旱試驗篩選帶有大量(Ethylene Response Factor)ERF轉錄因子的轉植T2阿拉伯芥種子,並且確認鑑定其中一個基因為ERF1,當該基因大量表現時,轉植株具有較佳的抗旱能力。於先前的報導中,ERF1(AT3G23240)被發現會受到Jasmonic acid (JA)與乙烯的誘導表現,並參與植株的抗病反應。然而ERF1在非生物逆境上的表現卻尚未被研究過。本篇研究發現,在乾旱以及高鹽逆境的測試中,ERF1的大量表現轉植株都較野生型有較高的存活率,同時,ERF1的RNA干擾轉植株存活率與野生型並無差異。ERF1大量表現的轉植株在高鹽環境下也較野生型有較高的萌芽率。透過GUS (β-glucuronidase)染色我們觀察到ERF1主要表現於葉尖及維管束中。同時,在氣孔孔徑的實驗中,ERF1大量表現轉植株的氣孔張開程度也較野生型來得小。而水分散失實驗中,ERF1大量表現轉殖株的水分散失速率相較野生型來得低。在乾旱以及高鹽逆境下,ERF1大量表現轉殖株累積較多的脯胺酸以維持細胞內膨壓。透過微陣列分析,我們挑選出在ERF1大量表現轉植株中正向調控的數個基因,這些基因皆被報導過具有增進阿拉伯芥對於乾旱,高鹽以及生物性逆境的耐受性。於此,我們的結果顯示ERF1增進乾旱和高鹽逆境的耐受力,同時在阿拉伯芥的生物性及非生物逆境兩條訊息傳遞中扮演交會點的功能。 Abiotic stresses, including salinity and drought, limit crop productivity and affect the expression of many genes in plants. Regulation of genes at transcriptional level has been described in response to environmental stresses. Particularly, many transcription factor (TF) genes are stress inducible, and function in regulating stress signal transduction pathways. Here, we screened a pool of ERF transcription factor transgenic T2 seeds of Arabidopsis thaliana by dehydration test, and identified a gene, Ethylene Response Factor 1 (ERF1,AT3G23240), which is resistant to drought stress. In previous report, ERF1, as a transcription factor, was activated by jasmonic acid (JA) and ethylene, and involved in pathogen resistance. The drought and salt test results showed that ERF1 overexpressing (OE) lines are more tolerant than wild-type (WT) plants. However, ERF1 RNAi transgenic lines showed no difference from WT in response to drought and salt. The ERF1 OE lines also had higher germination rate under salt stress compared with WT. The GUS (β-glucuronidase) staining showed ERF1 mainly expresses in vascular bundles. In stomatal aperture experiments, ERF1 OE lines plants showed a higher sensitivity to the application of ABA for its induction of stomatal closure. ERF1 OE lines also displayed less transpirational water loss. ERF1 OE lines accumulated more proline than WT under salt and drought stresses. By using microarray analysis, we found several genes up-regulated in the ERF1 OE lines, which have known functions in salt and drought stress tolerance. Our results suggested that ERF1 may play a positive role to enhance tolerance of salt and drought stresses, and a merging point of biotic and abiotic signaling. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27285 |
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顯示於系所單位: | 植物科學研究所 |
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