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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 許圳塗(Chou-Tou Shii) | |
| dc.contributor.author | Chun-Bin Chen | en |
| dc.contributor.author | 陳春賓 | zh_TW |
| dc.date.accessioned | 2021-06-12T17:56:46Z | - |
| dc.date.available | 2008-02-01 | |
| dc.date.copyright | 2008-02-01 | |
| dc.date.issued | 2008 | |
| dc.date.submitted | 2008-01-30 | |
| dc.identifier.citation | 六、參考文獻
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/27160 | - |
| dc.description.abstract | 百合遠緣雜種選用己育成之LA〔L. longiflorum cv.Gelria (LL)× L. ‘Purple Sensation’ Asiatic Lily(AA)〕及LO〔L. longiflorum LD(LL) ×L. ‘Casa blanca’ Oriental Lily(OO)〕選系，花粉母細胞染色體減數分裂檢查，第一次減數分裂前期L和A或L和O基因組間近同源染色體配對頻率低，同時呈鬆弛狀配對，易早熟分離。LA及LO選系在第一次減數分裂中期出現16-24個單價體(univalents)為主其12對近同源染色體配對頻率分別為4.18± 2.69 (Ⅱ)與1.04 ± 1.25 (Ⅱ)。顯示L與O兩基因組間遺傳親緣性為較低，第一次減數分裂後期，因近同源單價染色體無法完全配對及無法對稱分離，致使雙隅子(dyad)遺傳不平衡。接續第二分裂異常，減數分裂產物大小不一，同時數目會超過四分子，小孢子呈不整數染色體組無法形成有機能花粉，經由化學染色、螢光檢查及發芽測試皆顯示沒有活力。參試LA及 LO選系微體繁殖，為利用試管內保存小植株之小鱗片為培殖體，接種於1/2 MS培養基，分別添加2,4-D 0.1及0.5 mg / L，可誘導多數不定芽再生。兩選系小鱗片縱切體外誘變預處理，為接種於修正1/2 MS配方，添加不同濃度Oryzalin 7~28 μΜ培養液，30~90分鐘，爾後移至再生培養基，誘導不定芽，長成小植株並經氣孔形態調查及流式細胞儀細胞核DNA含量分析。初代誘變系第一輪篩檢LA及LO百合氣孔保衛細胞形態，結果顯示，氣孔形態平均分別為長 × 寬為101.4 ± 6.68 μm × 21.2 ± 1.9 μm及長 × 寬為105.06 ± 5.21μm × 21.05 ± 3.37 μm；分別為兩倍種1.65 × 1.45倍及1.4 × 1.29倍，取得四倍體細胞鑲嵌株誘變系頻率分別為46.9 ％ 和 57.9 ％，總鑲嵌頻度變化11.1~71.9 ％，LA及LO誘變系DNA含量篩檢分別為4C=170.82及4C=167.07，均為2C之2倍。選取篩檢值為4C單peak DNA含量四倍體LA及LO百合誘變系為5株誘變系及16株誘變系。第二輪檢定第一輪篩選出21株具單peak 4C DNA檢測值之四倍體LA及LO百合誘變系，篩檢其新生長且成熟葉片交互檢測形態誌、核DNA含量及根尖細胞學檢查(4X=48)，確認取得實質四倍體誘變株分別為1株及4株，誘變率分別為1.23 %及 2.37 %，由第二輪篩檢結果顯示，以浸漬90分鐘、Oryzalin濃度為28 μΜ之試驗處理組合為佳。誘變系再生二代第一輪及第二輪篩檢，分別取得不同培殖體鑲嵌誘變系，並分別自15株及82株鑲嵌誘變系純化篩檢獲得1株及7株再生二代四倍體誘變系，鑲嵌誘變系分離純化效率分別為6.67 %及8.54 %。植株氣孔密度分別為417.5 ± 65.0 / cm2(個) 及401.7 ± 52.1 / cm2(個)，分別約為兩倍體種的0.56倍及0.53倍，且根尖細胞學檢查均為4X等於48條染色體。誘變株LA及LO核DNA質量比對百合L. davidii細胞，分別估算為4C=159.57 ± 9.14 pg及4C=141.04 ± 4.67 pg亦接近為原雜種兩倍。檢定為實質四倍體各誘變系已微體繁殖中，供瓶外栽培及生長勢評估。 | zh_TW |
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| dc.description.tableofcontents | 國立臺灣大學(碩)博士學位論文口試委員會審定書…………….…...….i
中文摘要………………………………………………………….....…….iii Abstract………………………………………………………………….....v 目錄………………………………………………………………………...1 一、 前言...............................................................................................11 二、 前人研究.......................................................................................13 （一）、多倍體化的重要性.........................................................................13 （二）、有性多倍體之未減數配子形成(sexual, meiotic polyploidization)及其遺傳特性.....................................................................................14 1.減數分裂復合...................................................................................15 2.巨型孢子(Jumbo pollen)之形成.......................................................15 3.減數分裂後複合(Post meiotic restitution)………………...………15 （三）、無性多倍體途徑(asexual, mitotic polyploidization)……...……..16 1.自然生成……………………………………………………...........16 2.人為操作…………………………………………….…..….……...16 （1.）Oryzalin誘變之調控機制………………………….….…….17 （2.）誘變劑對kinetochore於細胞分裂時之作用機制與倍數體化之關係………………………..……………………………..19 i. Kinetochore之組成………………………………..……….19 ii. Chenkpoint Mad2…………………………………..….......20 iii. Mad2之兩種機能模式………………………….………..21 （四）、百合遠緣雜種體外多倍體誘導…………………………..…….21 三、 材料與方法……………………………………………….……..24 (一) 遠緣雜種LA及LO百合花粉活力分析………….….……………24 (二) 遠緣雜種LA及LO百合微體繁殖……………………..…………25 (三) 遠緣雜種LA及LO百合體外多倍體誘導試驗…………………..25 (四) 遠緣雜種LA及LO百合多倍體檢定及相關植株性狀調查……26 1.多倍體植株檢定…………………………………………….……26 (1.) 初代擬純質多倍體第一輪篩檢循環…………….....…….26 i. 細胞形態檢定…………………………………….…….26 ii. DNA相對含量檢定……………………………..……..27 (2.) 初代實質多倍體第二輪篩檢循環………………………28 i. 細胞學根尖染色體數檢查……………………..………28 (3.) 鑲嵌誘變系分離純化再生二代擬純質多倍體第一輪篩檢循環………………………………………………………..29 (4.) 鑲嵌誘變系分離純化再生二代實質多倍體第二輪篩檢循環…………………………………………………………..29 2.植株性狀調查……………………………………………….……30 (1.)葉片壽命……………………………………………..……..30 (2.)葉片之厚度測定…………………………………......……..30 (3.)氣孔之密度測定…………………………………...….........30 四、 結果………………………………………………………...……31 (一)遠緣雜種LA及LO二倍體百合花粉稔實率分析………………..31 (二)遠緣雜種LA及LO百合微體繁殖……………………..................32 (三) LA及LO遠緣雜種百合多倍體篩檢及相關植株性狀調查……..33 1. 多倍體誘變系篩檢…………………………………….………33 (1.) 初代擬純質多倍體第一輪篩檢循環……………………34 i.多倍體百合誘變系氣孔保衛細胞形態篩檢……........34 ii.百合誘變多倍體DNA含量篩檢…………………….35 (2.) 初代擬純質多倍體第二輪篩檢循環……………………35 i. 細胞學根尖染色體數篩檢…………………………..35 (3.) 鑲嵌誘變系分離純化再生二代擬純質多倍體第一輪篩檢循環………………………………………………………36 (4.) 鑲嵌誘變系分離純化再生二代實質多倍體第二輪篩檢循環…………………………………………………………36 2.植株性狀調查…………………………………………...………38 (1.) 葉片厚度測定分析……………………………......……..38 (2.) 氣孔密度測定分析……………………………….....…...38 (3.) 葉片壽命記錄分析……………………………......……..38 五、 討論……………………………………………………...………40 (一) 二倍體遠緣雜種百合花粉近同源配對特性及花粉稔實率分析………………………………………………………………...40 (二) 強效生長調節劑對遠緣雜種百合微體繁殖增殖特性………………………………………………………………...41 (三) 遠緣雜種LA及LO百合多倍體誘變系篩檢及植株特性………………………………………………………………...42 1百合多倍體誘變系細胞形態氣孔保衛細胞篩檢……...……...42 2.百合多倍體誘變系DNA含量篩檢……..…………………….43 (1.) 非包覆型鑲嵌誘變系植株………………………………44 (2.) 包覆型鑲嵌誘變系植株…………………………………44 3.百合多倍體誘變系細胞學根尖染色體數篩檢……………….45 4.二倍體及四倍體百合植秼性狀….……………………………47 六、 參考文獻…………………………..…………………………….98 附表………………………………………………………………...107 附圖………………………………………………………………...109 | |
| dc.language.iso | zh-TW | |
| dc.subject | 流式細胞儀 | zh_TW |
| dc.subject | Oryzalin | zh_TW |
| dc.subject | 離體多倍體誘變 | zh_TW |
| dc.subject | 2n未減數配子 | zh_TW |
| dc.subject | 遠緣雜種百合 | zh_TW |
| dc.title | 百合遠緣雜種LA及LO之稔性特性及多倍體誘導 | zh_TW |
| dc.title | Meiotic behavior and polyploid induction of LA and LO hybrid lilies | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 96-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李金龍(Ching-Lung Lee),黃鵬林(Pung-Ling Huang),金石文(Shih-Wen Chin),張耀乾(Yao-Chien Alex Chang) | |
| dc.subject.keyword | 離體多倍體誘變,2n未減數配子,遠緣雜種百合,Oryzalin,流式細胞儀, | zh_TW |
| dc.subject.keyword | in vitro polyploidization,2n gamete,hybrid lilium,Oryzalin,Flowcytometry, | en |
| dc.relation.page | 109 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2008-01-31 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 園藝學研究所 | zh_TW |
| 顯示於系所單位： | 園藝暨景觀學系 | |
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