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???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
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dc.contributor.advisor | 李佳音 | |
dc.contributor.author | Yuh-Wen Shen | en |
dc.contributor.author | 沈昱彣 | zh_TW |
dc.date.accessioned | 2021-06-08T07:32:19Z | - |
dc.date.copyright | 2011-08-10 | |
dc.date.issued | 2011 | |
dc.date.submitted | 2011-08-09 | |
dc.identifier.citation | 王韻婷 (2000). 高分子生物塑膠生產菌之分離,特性分析及其高分子合成基因之選殖. 國立台灣大學農業化學系碩士論文
黃筵嘉 (2005). 篩選特殊單體組成之具羥基烷酸酯生產菌並分析其PHA合成酶基質專一性. 國立台灣大學農業化學系碩士論文 楊勝斌 (2005). 以發酵法生產聚官能基之聚酯材料. 國立台灣大學材料科學與工程學系碩士論文 大西正躬,史蒂芬W米勒,儲存超高分子量聚酯之微生物。中華民國發明專利第200720429號說明書 賴美津,徐善慧,以極端嗜鹽古生菌生產新型聚酯。中華民國發明專利第579390號 賴美津,徐善慧,一種古生菌型聚酯做為生醫材料的應用。中華民國發明專利第I270376號 Abraham, G.A., Gallardo, A., San Roman, J., Olivera, E.R., Jodra, R., Garcia, B., Minambres, B., Garcia, J.L., and Luengo, J.M. (2001). Microbial synthesis of poly(beta-hydroxyalkanoates) bearing phenyl groups from pseudomonas putida: chemical structure and characterization. Biomacromolecules 2, 562-567. Arkin, A.H., and Hazer, B. (2002). Chemical modification of chlorinated microbial polyesters. Biomacromolecules 3, 1327-1335. Brandl, H., Gross, R.A., Lenz, R.W., and Fuller, R.C. (1988). Pseudomonas oleovorans as a source of poly(beta-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl Environ Microb 54, 1977-1982. Chen, G.Q. (2009). A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry. Chem Soc Rev 38, 2434-2446. Chen, G.Q., Wang, H.H., Zhou, X.R., and Liu, Q.A. (2011). Biosynthesis of polyhydroxyalkanoate homopolymers by Pseudomonas putida. Appl Microbiol Biot 89, 1497-1507. Chung, D.M., Choi, M.H., Song, J.J., Yoon, S.C., Kang, I.K., and Huh, N.E. (2001). Intracellular degradation of two structurally different polyhydroxyalkanoic acids accumulated in Pseudomonas putida and Pseudomonas citronellolis from mixtures of octanoic acid and 5-phenylvaleric acid. Int J Biol Macromol 29, 243-250. Dewaard, P., Vanderwal, H., Huijberts, G.N.M., and Eggink, G. (1993). Heteronuclear Nmr Analysis of Unsaturated Fatty-Acids in Poly(3-Hydroxyalkanoates) - Study of Beta-Oxidation in Pseudomonas-Putida. J Biol Chem 268, 315-319. Dufresne, A., Reche, L., Marchessault, R.H., and Lacroix, M. (2001). Gamma-ray crosslinking of poly(3-hydroxyoctanoate-co-undecenoate). Int J Biol Macromol 29, 73-82. Elbahloul, Y., and Steinbuchel, A. (2009). Large-scale production of poly(3-hydroxyoctanoic acid) by Pseudomonas putida GPo1 and a simplified downstream process. Appl Environ Microbiol 75, 643-651. Hazer, B., Lenz, R.W., and Fuller, R.C. (1994). Biosynthesis of Methyl-branched poly(beta-hydroxyalkanoate)S by Pseudomonas oleovorans. Macromolecules 27, 45-49. Hazer, B., and Steinbuchel, A. (2007). Increased diversification of polyhydroxyalkanoates by modification reactions for industrial and medical applications. Appl Microbiol Biot 74, 1-12. Ho, I.C., Yang, S.P., Chiu, W.Y., and Huang, S.Y. (2007). Structure and polymer form of poly-3-hydroxyalkanoates produced by Pseudomonas oleovorans grown with mixture of sodium octanoate/undecylenic acid and sodium octanoate/5-phenylvaleric acid. Int J Biol Macromol 40, 112-118. Ishizaki, A., Tanaka, K., and Taga, N. (2001). Microbial production of poly-D-3-hydroxybutyrate from CO2. Appl Microbiol Biotechnol 57, 6-12. Kamachi, M., Zhang, S.M., Goodwin, S., and Lenz, R.W. (2001). 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Poly-3-Hydroxyalkanoates Containing Unsaturated Repeating Units Produced by Pseudomonas-Oleovorans. J Polym Sci Pol Chem 33, 1367-1374. Kim, Y.B., Rhee, Y.H., Han, S.H., Heo, G.S., and Kim, J.S. (1996). Poly-3-hydroxyalkanoates produced from Pseudomonas oleovorans grown with omega-phenoxyalkanoates. Macromolecules 29, 3432-3435. Kocer, H., Borcakli, M., Demirel, S., and Hazer, B. (2003). Production of bacterial polyesters from some various new substrates by Alcaligenes eutrophus and Pseudomonas oleovorans. Turk J Chem 27, 365-373. Lageveen, R.G., Huisman, G.W., Preusting, H., Ketelaar, P., Eggink, G., and Witholt, B. (1988). Formation of Polyesters by Pseudomonas oleovorans: Effect of Substrates on Formation and Composition of Poly-(R)-3-Hydroxyalkanoates and Poly-(R)-3-Hydroxyalkenoates. Appl Environ Microbiol 54, 2924-2932. Lutke-Eversloh, T., Fischer, A., Remminghorst, U., Kawada, J., Marchessault, R.H., Bogershausen, A., Kalwei, M., Eckert, H., Reichelt, R., Liu, S.J., et al. (2002). Biosynthesis of novel thermoplastic polythioesters by engineered Escherichia coli. Nat Mater 1, 236-240. Martin, D.P., and Williams, S.F. (2003). Medical applications of poly-4-hydroxybutyrate: a strong flexible absorbable biomaterial. Biochem Eng J 16, 97-105. Noda, I., Green, P.R., Satkowski, M.M., and Schechtman, L.A. (2005). Preparation and properties of a novel class of polyhydroxyalkanoate copolymers. Biomacromolecules 6, 580-586. Philip, S., Keshavarz, T., and Roy, I. (2007). Polyhydroxyalkanoates: biodegradable polymers with a range of applications. J Chem Technol Biot 82, 233-247. Preusting, H., Nijenhuis, A., and Witholt, B. (1990). Physical characteristics of poly(3-Hydroxyalkanoates) and poly(3-Hydroxyalkenoates) produced by Pseudomonas-oleovorans grown on aliphatic-hydrocarbons. Macromolecules 23, 4220-4224. Reddy, S.V., Thirumala, M., and Mahmood, S.K. (2009). A novel Bacillus sp. accumulating poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from a single carbon substrate. J Ind Microbiol Biotechnol 36, 837-843. Reis, M.A.M., Albuquerque, M.G.E., Martino, V., Pollet, E., and Averous, L. (2011). Mixed culture polyhydroxyalkanoate (PHA) production from volatile fatty acid (VFA)-rich streams: Effect of substrate composition and feeding regime on PHA productivity, composition and properties. Journal of Biotechnology 151, 66-76. Rhee, Y.H., Kim, D.Y., Kim, H.W., and Chung, M.G. (2007). Biosynthesis, modification, and biodegradation of bacterial medium-chain-length polyhydroxyalkanoates. Journal of Microbiology 45, 87-97. Roy, I., Akaraonye, E., and Keshavarz, T. (2010). Production of polyhydroxyalkanoates: the future green materials of choice. J Chem Technol Biot 85, 732-743. Shah, D.T., Tran, M., Berger, P.A., Aggarwal, P., Asrar, J., Madden, L.A., and Anderson, A.J. (2000). Synthesis and properties of hydroxy-terminated poly(hydroxyalkanoate)s. 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Biomedical applications of polyhydroxyalkanoates: an overview of animal testing and in vivo responses. Expert Rev Med Devices 3, 853-868. van der Walle, G.A., de Koning, G.J., Weusthuis, R.A., and Eggink, G. (2001). Properties, modifications and applications of biopolyesters. Adv Biochem Eng Biotechnol 71, 263-291. Wampfler, B., Ramsauer, T., Rezzonico, S., Hischier, R., Kohling, R., Thony-Meyer, L., and Zinn, M. (2010). Isolation and Purification of Medium Chain Length Poly(3-hydroxyalkanoates) (mcl-PHA) for Medical Applications Using Nonchlorinated Solvents. Biomacromolecules 11, 2716-2723. Wang, H.H., Li, X.T., and Chen, G.Q. (2009). Production and characterization of homopolymer polyhydroxyheptanoate (P3HHp) by a fadBA knockout mutant Pseudomonas putida KTOY06 derived from P. putida KT2442. Process Biochem 44, 106-111. Ward, P.G., de Roo, G., and O'Connor, K.E. (2005). Accumulation of polyhydroxyalkanoate from styrene and phenylacetic acid by Pseudomonas putida CA-3. 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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26918 | - |
dc.description.abstract | 聚羥基烷酸酯Polyhydroxyalkanoate(PHA),是微生物用來儲存碳源及能量之物質型式。本研究室分離出一株Pseudomonassp. TO7,已證實為中長鏈PHA生產菌。本篇論文研究目的探討當提供具特殊官能基例如苯環、不飽和單元之基質,當做碳源培養Pseudomonas sp. TO7,菌體能否累積生產支鏈帶有特殊官能基之PHA,並分析這些新穎PHA的特性。在鹽類培養基(mineral salt midium)中添加混合碳源基質辛酸鈉(sodium octanoate)與具雙鍵之十一烯酸(11-undecylenic acid),或壬酸(nonanoic acid)與具雙鍵之十一烯酸,在固定總碳源為0.5%情況下,調整兩種碳源莫耳比例為2:1、1:1、1:2,培養Pseudomonas sp. TO7 於28oC,pH7.3二至三天,將菌體產生之PHA萃取出,進一步進行分子量及物性分析。此外提供碳源基質辛酸鈉濃度為0.5%,混合不同濃度0.1- 0.4%的苯戊酸(5-phenylvaleric acid)於培養基中,將Pseudomonas sp. TO7培養在28oC下進行含苯環PHA累積後,同樣進行PHA萃取並以分子量及物性分析。各項菌體累積所產生的PHA經過萃取後進行1H及13C NMR分析,NMR結果證實各種PHA結構確實符合預期。以辛酸鈉或壬酸搭配十一烯酸混合碳源,生產出之PHA帶有雙鍵,分別為P(HO-co-HU)及P(HN-co-HU),分子量幾乎都為二十萬道耳頓左右(Da),且分子量分部指數(PDI)接近2-3。以辛酸鈉與苯戊酸為混合碳源培養後,菌體生產出含苯環的PHA為P(HO-co-HPV),PHA分子量為十萬(Da),分子量分布指數約2.4。所生產之P(HO-co-HU)、P(HN-co-HU) 熔點(Tm)值皆為50oC左右,玻璃轉換溫度(Tg)值則為-50oC;而P(HO-co-HPV)之玻璃轉換溫度為-30oC,熔點則為44- 71oC。 控溫式傅利葉紅外線光譜儀(FT-IR)分析結果亦證實,所生產之PHA確實具有不飽和單元與含苯環單元。結果顯示可藉由提供TO7混合碳源,生成具有相關特殊單體之PHA。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-08T07:32:19Z (GMT). No. of bitstreams: 1 ntu-100-R98623024-1.pdf: 3210704 bytes, checksum: f51439cf80682cf0887123fd8890075a (MD5) Previous issue date: 2011 | en |
dc.description.tableofcontents | 口試委員會審定書....................................................................................... i
中文摘要.......................................................................................................ii 英文摘要......................................................................................................iv 目錄..............................................................................................................vi 表次...............................................................................................................x 圖次.............................................................................................................xii 附錄表次....................................................................................................xiv 附錄圖次.....................................................................................................xv 縮寫表........................................................................................................xvi 壹、前言..........................................................................................................1 一、PHA介紹 1 1.1 PHA概述 1 1.2 PHA之化學結構 2 1.3 PHA代謝途徑 2 1.4 PHA特性介紹 3 二、新穎PHA 4 1.1傳統PHA與傳統塑膠比較 4 1.2生產新穎PHA目的 4 1.3新穎PHA 5 1.4新穎PHA相關專利研究 9 三、PHA目前應用範圍 11 1.1PHA農業應用 11 1.2PHA工業應用 12 1.3PHA生醫材料應用 13 1.4PHA相關其它應用 14 四、PHA未來展望 15 1.1尋找替代碳源 15 五、研究目的 15 貳、實驗材料與方法....................................................................................16 一、實驗材料 16 1.1實驗菌株 16 1.2培養基 16 1.3藥品與試劑 17 1.4儀器 18 二、實驗方法 19 1.1 PHA累積實驗 19 1.2各種碳源選擇及調整各種碳源比例生產PHA 19 1.3最適PHA累積時間 20 1.4菌體乾重、PHA濃度、PHA含量分析 20 1.5萃取PHA 20 1.6 Nile red染色法證明PHA累積 21 1.7超導核磁共振NMR(Nuclear magnetic resonance) 21 1.8凝膠滲透層析(Gel permeation chromatography) 22 1.9微差熱掃描分析儀(Differential scanning calorimetry) 22 1.10控溫式傅利葉轉換紅外線光譜儀(Fourier transform infrared spectroscopy) 22 參、實驗結果................................................................................................23 一、生成各種新穎PHA之碳源篩選 23 1.1 Pseudomonas sp. TO7為PHA累積菌 23 1.2碳源選擇 23 1.2.1單一碳源 23 1.2.2混合碳源 24 二、 大量培養PHA及各項物性分析 25 1.1細胞乾重與PHA含量觀察 25 1.2以NMR證實PHA結構及PHA組成 26 1.3以凝膠滲透層析儀GPC(Gel permeation chromatography)進行分子量分析 28 1.4以微差熱掃描分析儀DSC(Differential scanning calorimetry)進行熱分析 29 1.5以控溫式傅利葉轉換紅外線光譜儀FT-IR(Fourier transform infrared spectroscopy)分析結構 30 肆、討論........................................................................................................31 一、混合碳源中Pseudomonas sp. TO7的生長與PHA累積 31 二、生成之PHA單體含量及其特性 31 三、 PHA產率 33 四、物性分析代表之意義 34 五、依照物性分析選擇最適PHA材料 34 伍、結論.......................................................................................................36 陸、參考文獻...............................................................................................38 柒、表...........................................................................................................48 捌、圖...........................................................................................................64 玖、附錄表...................................................................................................83 拾、附錄圖...................................................................................................87 | |
dc.language.iso | zh-TW | |
dc.title | Pseudomonas sp. TO7由混合碳源生產具官能基之聚羥基烷酸酯 | zh_TW |
dc.title | The production of PHA bearing functional group by Pseudomonas sp. TO7 with mixture carbon source | en |
dc.type | Thesis | |
dc.date.schoolyear | 99-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊美桂,劉啟德 | |
dc.subject.keyword | 聚羥基烷酸酯,官能基, | zh_TW |
dc.subject.keyword | Pseudomonas,Polyhydroxyalkanoate,Functional group, | en |
dc.relation.page | 88 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2011-08-09 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
Appears in Collections: | 農業化學系 |
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