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標題: | 晚熟鳥錦花雀與早熟鳥雞在初生絨毛發育上基因調控之差異 Regulatory Differences in Natal Down Development between Altricial Zebra Finch and Precocial Chicken |
作者: | Chih-Kuan Chen 陳志冠 |
指導教授: | 丁照棣(Chau-Ti Ting) |
關鍵字: | 早熟鳥,晚熟鳥,羽毛演化,絨羽,錦花雀, altricial bird,precocial bird,feather evolution,natal down,zebra finch, |
出版年 : | 2017 |
學位: | 博士 |
摘要: | 鳥類可以分成晚熟與早熟鳥兩個大群,晚熟鳥的幼雛通常絨毛稀疏而皮膚裸露,早熟鳥則通常全身覆蓋著絨毛。一般認為,這種形態上的差異是跟環境的適應有關,但是背後的分子機制仍然不明朗。在本研究中,我以晚熟的錦花雀以及早熟的雞作為研究的模式物種。錦花雀的幼雛具有皮膚裸露的前背部,以及部份長有絨毛的後背部。藉由高通量的轉錄組定序與分析,我比較了前背部與後背部的基因表現,結果發現促進羽毛生長相關的基因SHH (sonic hedgehog)在後背部有較高的表現量。此外,資料分析也顯示FGF/MAPK訊息路徑可能跟前背部絨毛的生長抑制相關,而FGF16 (fibroblast growth factor 16)可能是這個訊息路徑的上游調控因子。將FGF16以病毒表現系統異位表現於雞胚胎的腿部,結果該部位的絨毛生長被抑制,出現類似錦花雀胚胎前背部的樣式,而且該部位SHH的表現量減少,又一個已知的羽毛抑制因子FGF10的表現量則增加。因此,我認為FGF16相關的分子訊息抑制了絨毛的生長,進而造成了錦花雀幼雛裸露的前背部。本研究闡述了早晚熟鳥之間絨毛生成可能的基因調控差異。 Birds can be classified into altricial and precocial. The hatchlings of altricial birds are almost naked, whereas those of precocial birds are covered with natal down. This regulatory divergence is thought to reflect environmental adaptation, but the molecular basis of the divergence is unclear. To address this issue, I chose the altricial zebra finch and the precocial chicken as the model animals. Anatomical analysis revealed that zebra finch hatchlings showed suppressed natal down growth in anterior dorsal (AD) skin but partially down-covered posterior dorsal (PD) skin. A comparison of the transcriptomes of AD and PD skins revealed a higher expression level of the feather growth promoter SHH (sonic hedgehog) in PD skin than in AD skin. Moreover, the data suggested that the FGF/MAPK signaling pathway is involved in natal down growth suppression and that FGF16 (fibroblast growth factor 16) is an upstream signaling suppressor. Ectopic expression of FGF16 on chicken leg skin showed downregulation of SHH, upregulation of the feather growth suppressor FGF10, and suppression of feather bud elongation, similar to the phenotype found in zebra finch embryonic AD skin. Therefore, I propose that FGF16 related signals suppress natal down elongation and cause the naked AD skin in zebra finch. This study provides insights into the regulatory divergence in natal down formation between precocial and altricial birds. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/2690 |
DOI: | 10.6342/NTU201700010 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 生態學與演化生物學研究所 |
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檔案 | 大小 | 格式 | |
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ntu-106-1.pdf | 3.72 MB | Adobe PDF | 檢視/開啟 |
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