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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 徐源泰 | |
dc.contributor.author | Yi-Ru Hsieh | en |
dc.contributor.author | 謝易儒 | zh_TW |
dc.date.accessioned | 2021-06-08T07:20:40Z | - |
dc.date.copyright | 2008-07-30 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-23 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26682 | - |
dc.description.abstract | Cellulase plays an important role in the degradation and recycle of fiber wastes. Conversion of cellulose has great potential in biomass application such as energy. This study has investigated cellulase gene from a halophilic strain Vibrionaceae sp. NTU 05, isolated from saline area in southern Taiwan. Cellulase gene from the bacteria has been cloned and heterogeneous expressed for application in simultaneous hydrolysis and fermentation. This study has amplified cellulase gene from Vibrionaceae sp. NTU05 genomic DNA containing 1,232 bp by PCR using CELCF / CELCR primer, which was suitable in the amplification of celC in Bacillus licheniformis. The target gene contains a complete opening reading frame composes of 807 nucleotide that translates 269 amino acid residues that showed 96 % homology to Bacillus licheniformis celC (AM183792.1). The celC was ligated with pGEM®-T easy vector and further transformed in E. coli DH5α. The celC was subsequently subcloned into the pET30a(+)expression vector, and expressed it in Escherichia coli BL21 (DE3). The transformant T1 was cultivated on the LB-CMC plate and detected by Congo red test. Hydrolysis halo is clearly been observed around the colony. The activity was determined at 63.7 mU / mL in intracellular, and no activity was detected in extracellular. The enzyme is specific for the substrate of β-1,4 -linkages of CMC and β-1,3、β-1,4 –linkages of β-glucan. The optimal temperature and pH value for activity of enzyme are detected at 40℃ and pH 7.0, respectively. The activity remained at 80 % when 5 % salinity was given, and retained higher than 70% in 10 % salinity environment for more than 1 hour. The protein was analyzed by fast protein liquid chromatography (FPLC). The purified products were analyzed by native-PAGE, which showed a molecular weight of 29.5 kDa, as expected. This study has successfully established the cloning and heterogeneous expression system of the celC from Vibrionaceae sp. NTU 05. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T07:20:40Z (GMT). No. of bitstreams: 1 ntu-97-R95628205-1.pdf: 2141234 bytes, checksum: a147462519c24e3573f15ad368865bfe (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 中文摘要 I
英文摘要 II 目錄 III 圖目錄 VII 表目錄 IX 第一章 前言 1 第二章 文獻回顧 2 一、纖維素 2 1.纖維素之來源與功能 2 2.木質纖維生物質 2 3.纖維素之組成與結構 6 4.纖維素之資源化 9 二、纖維水解酶 10 1.纖維水解酶之定義 10 2.纖維水解酶之酵素分類 10 3.纖維素解酶之作用類型 10 4.自然界中纖維水解酶之來源 14 5.纖維水解酶之工業應用 16 三、Vibrionaceae sp. NTU 05纖維水解酶基因之選殖與表現 20 1.極端嗜鹽菌Vibrionaceae sp. NTU 05之簡介 20 2.基因選殖與表現系統之建構 23 (1)宿主細胞 23 (2)選殖與表現載體 27 (3)影響轉形株表現之因素 28 第三章 研究動機與預期目標 30 第四章 材料與方法 33 第一節 實驗材料 33 一、使用之菌株、載體 33 二、使用之試劑與藥品 33 三、藥品與培養基配方 37 四、使用之儀器與設備 39 第二節 實驗方法 40 一、菌種之保存與活化 40 二、嗜鹽菌Vibrionaceae sp. NTU 05纖維水解酶基因celC之選殖 41 1.引子設計 41 2.染色體DNA之製備 41 3. DNA之濃縮與純化 42 4.DNA濃度之測定 42 5.聚合酶鏈連鎖反應 42 6.DNA定序與序列分析比對. 43 7.DNA之電泳分析 44 8.洋菜膠體回收PCR產物DNA 44 9.選殖系統之構築 45 10.重組質體之檢視 45 三、嗜鹽菌Vibrionaceae sp. NTU 05纖維水解酶基因celC之表現 46 1.小量質體之製備 46 2.質體DNA之限制酶處理 47 3表現系統之建構 47 4轉形株之平板活性檢測 48 四、T1轉形株重組纖維水解酶CelC之分析與純化 49 1.重組纖維水解酶粗萃液之製備 49 2.蛋白質定量 49 3.蛋白質濃縮 49 4.酵素分離與純化 50 5.蛋白質聚丙烯醯胺膠體電泳分析 50 6.蛋白質活性電泳分析 52 六、T1轉形株纖維水解酶之活性分析 53 1.活性測定方法 53 2.基質特異性測定 54 3.酵素特性分析 54 第五章、結果與討論 56 第一節 引子設計與基因比對分析 56 一、 PCR選殖Vibrionaceae sp. NTU 05之纖維水解酶基因 56 二、基因分析與比對 56 三、基因之蛋白質功能性區域預測 57 第二節 Vibrionaceae sp. NTU 05纖維水解酶基因之選殖 59 一、選殖質體之構築 59 二、轉形株之檢測 59 第三節 Vibrionaceae sp. NTU 05.纖維水解酶基因之表現 60 一、表現質體之構築 60 二、轉形株之檢測 60 三、轉形株之活性篩選 60 四、轉形株之生長特性測定 61 第四節 T1轉形株纖維水解酶之純化 62 一、快速蛋白質液相層析 62 二、產物分子量大小與活性確認 62 第六節、T1轉形株纖維水解酶之特性分析 63 一、酵素活性分析 63 二、基質特異性分析 63 三、最適反應溫度測試 64 四、溫度穩定性測試 64 五、最適反應pH值測試 65 六、pH穩定性測試 65 七、最適反應鹽度 66 八、鹽度穩定性測試 66 第六章 結論 67 第七章 未來展望 69 參考文獻 70 附錄圖表 84 | |
dc.language.iso | zh-TW | |
dc.title | 嗜鹽菌Vibrionaceae sp. NTU 05纖維水解酶基因之選殖與表現 | zh_TW |
dc.title | Molecular cloning and expression of the cellulase gene from halophiles Vibrionaceae sp. NTU 05 | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陳昭瑩,曾文聖 | |
dc.subject.keyword | 纖維水解酶,嗜鹽性菌株,水解環,快速蛋白質液相層析系統, | zh_TW |
dc.subject.keyword | cellulase,halophilic strain,hydrolysis halo,fast protein liquid chromatography (FPLC), | en |
dc.relation.page | 82 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2008-07-25 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 園藝學研究所 | zh_TW |
顯示於系所單位: | 園藝暨景觀學系 |
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