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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 陳宏文 | |
dc.contributor.author | Liang-Fu Chen | en |
dc.contributor.author | 陳亮甫 | zh_TW |
dc.date.accessioned | 2021-06-08T07:19:35Z | - |
dc.date.copyright | 2008-07-30 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-24 | |
dc.identifier.citation | Antony JM, van Marle G, Opii W, Butterfield DA, Mallet F, Yong VW, Wallace JL, Deacon RM, Warren K, Power C. (2004) Human endogenous retrovirus glycoprotein-mediated induction of redox reactants causes oligodendrocyte death and demyelination. Nat Neurosci. 7:1088-1095.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26659 | - |
dc.description.abstract | 中文摘要
在胎盤發育過程中,細胞滋養層細胞會進行細胞與細胞間融合而形成多核的融合滋養層細胞,融合滋養層細胞是母體與胎兒物質交換的主要媒介,因此細胞滋養層細胞間的融合對於胎盤發育是必須的過程。在先前的研究中已知人類內生性反轉錄病毒HERV-W的外鞘蛋白syncytin 1可能參與及調控細胞滋養層細胞的融合,而近幾年所發現另外的人類內生性反轉錄病毒HERV-FRD的外鞘蛋白syncytin 2也被發現大量表現於胎盤,暗示著syncytin 2調控細胞滋養層細胞融合的可能。 在過去本實驗室對於syncytin 1研究中發現syncytin 1 C端胺基酸序列會抑制syncytin 1的融合功能,為了解syncytin 2在功能與結構上和syncytin 1的異同,本研究首先探究syncytin 2的C端序列是否也抑制syncytin 2 的融合功能,經融合檢驗發現syncytin 2的C端胺基酸序列並不會抑制其融合功能,反而對於syncytin 2調控細胞間融合是必須的。 在syncytin 1與syncytin 2的蛋白生合成過程中,本研究發現syncytin蛋白皆會被內生性蛋白酶Furin所剪裁修飾,而形成surface(SU)及transmembrane(TM)兩次單元,並且兩次單元藉由形成雙硫鍵連接,藉由突變SU次單元上CXXC的結構發現突變蛋白會喪失調控細胞與細胞間融合的功能,並且都具有顯性抑制效應,代表著syncytin 1與syncytin 2在和受器作用與調控細胞融合上有著相似的機制。 綜合以上結果可以了解syncytin 2與syncytin 1在蛋白生合成及融合機制上有許多相似,進一步的暗示syncytin 2與syncytin 1在胎盤發育上扮演相似角色的可能性,並有助於未來繼續研究和釐清syncytin蛋白在胎盤發育中所扮演的角色。 | zh_TW |
dc.description.abstract | Abstract
Fusion of cytotrophoblast into the multinucleated syncytiotrophoblast layer is essential for the development of a functional placenta. Previous studies of placental development have shown that the envelope protein of a human endogenous retrovirus W (HERV-W), syncytin 1, may mediate placental cell fusion. Recently, the envelope protein of HERV-FRD, syncytin 2, has been identified and shown to be highly expressed in placenta. This implies that syncytin 2 may be involved in placental cell fusion. Our previous studies have demonstrated that the cytoplasmic domain (CTM) of syncytin 1 inhibits its fusogenic activity. In this study, we first tested whether the CTM of syncytin 2 regulates its fusogenic activity. We found that the CTM of syncytin 2 is required for its fusogenic activity. We further studied the biosynthesis of syncytins and demonstrated that Furin is involved in the proteolytic cleavage of syncytin 1 and syncytin 2 by using furin inhibitor and in the Furin-defective human colon carcinoma LoVo cell line. Finally, we studied the role of the disulfide-forming CXXC and CX6CC motifs in syncytins and found that the disulfide linkage between SU and TM subunits is essential for the fusogenic activity of syncytins because mutation in the CXXC motif abolishes the fusogenic activity of syncytins and causes a dominant-negative effect. Overall, this study provides a better understanding of the biology of syncytin 1 and syncytin 2. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T07:19:35Z (GMT). No. of bitstreams: 1 ntu-97-R95b46027-1.pdf: 639983 bytes, checksum: b3203f7932297f25e8e54daf14a568f0 (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 目 錄
目錄………………………………………………………………………………………….I 圖表目錄……………………………………………………………………......................II中文摘要…………………………………………………………………………..………III 英文摘要…….................................................................................................................IV 第一章 序論 1.1 胎盤(Placenta)……………………………….………………………………...1 1.2 人類內生性反轉錄病毒(HERV)……………………………….……………4 1.3 Furin…………………………………………………..……………….…………7 1.4 研究動機及目標………………………………………………...…………….…7 第二章 材料與方法 2.1 重組質體構築………………….……………………………..…………………9 2.2 細胞實驗……………………………………….……..………………………..13 2.3 免疫沈澱法…………………………………………………………………….15 2.4 西方墨點法…………………………………………….…………………15 第三章 實驗結果 3.1 檢驗增加標籤對於Syncytin 1與Syncytin 2生物活性的影響………………17 3.2 Syncytin 2 C端胺基酸序列對於Syncytin 2融合功能的重要性…………….17 3.3 Furin對於Syncytin 1與Syncytin 2的剪裁修飾………………………………18 3.4 突變在CXXC結構對於syncytin 1與syncytin 2融合功能的影響…………20 3.5 Syncytin 1與Syncytin 2突變蛋白的顯性抑制效應………………………….21 第四章 總結與討論………………...…………………………………………………23 第五章 圖表………………………………...…………………………………………26 第六章 參考文獻……………………………………………...………………………33 | |
dc.language.iso | zh-TW | |
dc.title | 胎盤融合性蛋白syncytin 1和syncytin 2 的功能分析 | zh_TW |
dc.title | Functional analysis of the placental fusogenic proteins,syncytin 1 and syncytin 2 | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李明亭,黃銓珍,張震東,張功耀 | |
dc.subject.keyword | 胎盤,融合性, | zh_TW |
dc.subject.keyword | placenta,syncytin, | en |
dc.relation.page | 37 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2008-07-26 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科學研究所 | zh_TW |
顯示於系所單位: | 生化科學研究所 |
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