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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊台鴻 | |
dc.contributor.author | Hui-Ru Tu | en |
dc.contributor.author | 塗惠如 | zh_TW |
dc.date.accessioned | 2021-06-08T07:15:23Z | - |
dc.date.copyright | 2008-08-05 | |
dc.date.issued | 2008 | |
dc.date.submitted | 2008-07-28 | |
dc.identifier.citation | Lin, S.J., et al., Formation of melanocyte spheroids on the chitosan-coated surface. Biomaterials, 2005. 26(12): p. 1413-1422.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/26564 | - |
dc.description.abstract | Biomaterials are a very important field in tissue engineering. It has been shown that biomaterials can affect the morphology of cells. For certain cells, specific cellular function is determined by its intercellular organization. To be specific, the hair follicle (HF) induction activity of dermal papilla (DP) cells can only be preserved when they are kept in an aggregated state or microtissues.
We have demonstrated that DP cells can spontaneously grow into spheroidal microtissues on poly(ethylene-co-vinyl-alcohol) (EVAL) and these microtissues are able to induce HF neogenesis. However, DP cells have a compromised attachment on EVAL, and this may lead to cell loss during culture. In this work, we try to improve the cell attachment on EVAL by coating various extracellular matrix proteins to enhance cell attachment with the aim at more efficient production of DP microtissues on EVAL. The purpose of this research is to investigate the attachment, migration and aggregation of DP cells into microtissue on EVAL which is coated with various extracellular matrix proteins including fibronectin, collagen I, collagen IV and laminin. We found that cell attachment is enhanced by the coating of all the proteins tested. However, DP microtissue formation is only observed on the fibronectin-coated surface. We compare the cell mobility on the extracellualr matrix protein-coated surface and found that cell migration rate is reduced by the coating of collagen I, collagen IV and laminin while cells are maintained in a highly motile state on fibronectin-coated surface. In the case of the fibronectin-coated surface, there is a 66% increase of DP microtissue formation compared with that of non-coated EVAL. The increase in cell attachment and DP microtissue formation on the fibronectin-coated surface can be abolished by the treatment of fibronectin antibody. The result indicates that, in addition to cell-substrate adhesivity, cell migration is also a major determinant of microtissue formation. Each extracellualr matrix protein has different effects on DP cells attachment and migration on biomaterials. Cells can only grow into DP microtissues when the extracellualr matrix proteins can keep them in a more motile state. Overall, fibronectin-coating can enhance DP microtissue formation on EVAL by increasing cell-substrate adhesivity without compromising cell motility. Fibronectin-coating can improve the efficiency of DP microtissue production and can be applied to tissue engineering for HF regeneration. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T07:15:23Z (GMT). No. of bitstreams: 1 ntu-97-R95548051-1.pdf: 8107766 bytes, checksum: a2e30b060615f2bc75b0db13e4cc975a (MD5) Previous issue date: 2008 | en |
dc.description.tableofcontents | 致謝 I
Abstract II 中文摘要 V 目錄 VII 圖表目錄 X 第一章 序論 1 1.1. 研究背景跟動機 1 1.2. 禿髮 3 1.3. 禿髮的治療 4 1.3.1. Minoxidil 4 1.3.2. Finasteride 4 1.3.3. 植髮 4 1.4. 毛囊簡介 5 1.4.1. 毛囊解剖 5 1.4.2. 毛囊形態之形成 6 1.4.3. 毛髮週期 9 1.5. 毛囊的組織工程 11 1.6. 生醫材料簡介 13 1.6.1. 合成高分子 13 1.6.2. 天然高分子 14 1.7. 聚乙烯乙烯醇共聚物 poly (ethylene-co-vinyl-alcohol) (EVAL) 15 1.8. 細胞貼附、移行以及聚集 16 第二章 實驗目的及設計 18 2.1. 實驗目的 18 2.2. 實驗設計 18 第三章 材料與方法 19 3.1. 聚乙烯-乙烯醇製備 19 3.2. 聚乙烯-乙烯醇(EVAL)前處理 20 3.3. 毛囊的分離 20 3.4. 真皮乳頭的分離 20 3.5. 細胞培養 21 3.6. 細胞貼附及生長 22 3.7. MTT測試 23 3.8. 曠時攝影細胞移行以及聚集成球狀微組織動態實驗(Time-lapse microscope for cell migration assay and the aggregation of spheroidal microtissue) 24 3.9. 曠時攝影細胞移行距離影像分析 24 3.10. 測出使真皮乳頭微組織較快速增加之條件 25 3.11. 真皮乳頭微組織內細胞的存活率 27 第四章 結果 28 4.1. 細胞貼附 28 4.2. 細胞生長 33 4.3. 在EVAL上塗佈細胞外間質蛋白對真皮乳頭球狀微組織形成的影響 35 4.4. 細胞移行實驗(Cell migration) 41 4.5. 細胞聚集成球狀微組織動態過程 43 4.6. 真皮乳頭微組織內細胞的存活率 49 4.7. 功能性抑制fibronectin 51 4.7.1.測試fibronectin對於細胞貼附的影響 51 4.7.2. 測試fibronectin對於細胞聚集成球狀微組織個數的影響 53 4.7.3. 測試fibronectin對於細胞移行速率的影響 55 第五章 討論 57 第六章 結論 62 參考文獻 63 | |
dc.language.iso | zh-TW | |
dc.title | 塗佈細胞外間質蛋白質在乙烯-乙烯醇共聚物上對於誘導
真皮乳頭微組織形成的影響 | zh_TW |
dc.title | The effect of extracellular matrix proteins on poly (ethylene-co-vinyl-alcohol)-induced dermal papilla microtissue formation | en |
dc.type | Thesis | |
dc.date.schoolyear | 96-2 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 林頌然 | |
dc.contributor.oralexamcommittee | 張中興,黃敏銓,洪智煌 | |
dc.subject.keyword | 毛囊再生,真皮乳頭細胞,附著,乙烯-乙烯醇共聚物,細胞移行,組織工程,微組織, | zh_TW |
dc.subject.keyword | hair follicle regeneration,dermal papilla cells,adhesivity,poly (ethylene-co-vinyl alcohol),cell migration,tissue engineering,microtissue, | en |
dc.relation.page | 70 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2008-07-29 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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