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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 靳宗洛 | |
dc.contributor.author | Hui-Chuan Lai | en |
dc.contributor.author | 賴慧娟 | zh_TW |
dc.date.accessioned | 2021-06-08T06:57:36Z | - |
dc.date.copyright | 2009-07-22 | |
dc.date.issued | 2009 | |
dc.date.submitted | 2009-07-15 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25959 | - |
dc.description.abstract | 熱休克反應,為生物體內普遍存在的機制;藉由誘導熱休克蛋白質的生成,進行細胞保護,而熱休克蛋白質的生成是經由熱休克轉錄因子所調控。人類熱休克轉錄因子結合蛋白(HsHSBP1)可以和熱休克轉錄因子進行交互作用,終止熱休克反應,扮演負向調控者的角色。關於植物HSBP參與熱休克反應的機制目前並不清楚。藉由序列分析,我們在阿拉伯芥中篩選到一個HSBP基因(AtHSBP),並分離出兩個基因缺陷突變株。經由測試其熱耐受性,顯示突變株具有較高的「誘導耐熱性」,但是對於「先天耐熱性」並沒有顯著的影響。在阿拉伯芥原生質體中表現GFP-AtHSBP,得知在正常生長情況以及熱處理的初期,AtHSBP表現於細胞質中;但長時間的熱處理及當其回復到正常生長情況時,AtHSBP會自細胞質轉移至細胞核。雙分子螢光互補(Bimolecular fluorescence complementation)分析的結果顯示,AtHSBP會和AtHSFA1a, AtHSFA1b以及AtHSFA2進行交互作用。利用即時定量聚合酶鍊鎖反應(quantitative real-time PCR)分析,得知AtHSBP缺陷突變株在遭受熱逆境時,熱休克蛋白基因(HSP101, HSP70, HSP17.4, HSP18.2, and HSP25.3)的表現量及累積量比野生型多;而AtHSBP的過度表現株,其熱休克蛋白基因的表現量及累積量下降,並導致其「誘導耐熱性」能力降低。經由以上結果,我們推測AtHSBP在熱休克反應對於熱休克蛋白的表現,扮演負向調控者的角色。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-08T06:57:36Z (GMT). No. of bitstreams: 1 ntu-98-R95b42003-1.pdf: 1068496 bytes, checksum: ac57e5764f03aa570031c89f8622c673 (MD5) Previous issue date: 2009 | en |
dc.description.tableofcontents | Abstract in Chinese---------------------------------------------------------------------------- 1
Abstract in English---------------------------------------------------------------------------- 2 Abbreviations----------------------------------------------------------------------------------- 3 Introduction------------------------------------------------------------------------------------- 4 Heat shock response and heat shock proteins----------------------------------------------- 4 Regulation of heat shock response ----------------------------------------------------------- 5 Heat shock factors in Arabidopsis------------------------------------------------------------ 6 Previous study of heat shock factor binding proteins-------------------------------------- 8 Goals and strategies of the dissertation------------------------------------------------------ 9 Materials and Methods---------------------------------------------------------------------- 11 Plant material, transformation and growth condition ------------------------------------- 11 RNA extraction and quantitative real-time PCR ------------------------------------------ 11 Thermotolerance phenotypes assay --------------------------------------------------------- 12 Transient expression in Arabidopsis protoplast-------------------------------------------- 12 Subcellular localization and bimolecular fluorescence complementation assay ------13 Results------------------------------------------------------------------------------------------ 14 Sequence alignment and phylogenetic analysis of the HSBP homologs across plants and animals------------------------------------------------------------------------------------- 14 Expression profiles of AtHSBP in various tissues and with HS treatment ------------ 15 Thermotolerance analysis in AtHSBP mutants-------------------------------------------- 15 AtHSBP is a cytosolic-localized protein capable of entering nucleus in response to thermal stress----------------------------------------------------------------------------------- 16 AtHSBP interacts with AtHSFA1a, AtHSFA1b and AtHSFA2 in vivo----------------- 17 The HSP expression patterns in AtHSBP mutants during HSR------------------------- 18 Discussion-------------------------------------------------------------------------------------- 19 AtHSBP is a conserved protein in HSBP family and may participate in embryogenesis and environmental stress---------------------------------------------------------------------- 19 AtHSBP acts as a negative regulator during HSR----------------------------------------- 20 AtHSBP participates in the temporal and spatial regulation of HSP genes expression under the influence of heat stress------------------------------------------------------------ 22 Figures and Table----------------------------------------------------------------------------- 23 References-------------------------------------------------------------------------------------- 35 Supplemental data---------------------------------------------------------------------------- 40 | |
dc.language.iso | en | |
dc.title | 阿拉伯芥熱休克轉錄因子結合蛋白之功能性研究 | zh_TW |
dc.title | Functional Study of Heat Shock Factor Binding Protein in Arabidopsis thaliana | en |
dc.type | Thesis | |
dc.date.schoolyear | 97-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林秋榮,葉國楨,葉開溫,張英? | |
dc.subject.keyword | 熱休克轉錄因子結合蛋白,熱逆境,熱休克轉錄因子,阿拉伯芥, | zh_TW |
dc.subject.keyword | HSBP,heat stress,HSF,Arabidopsis, | en |
dc.relation.page | 47 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2009-07-16 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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