飼料添加重組類胰島素成長因子與植物性原料取代魚粉對赤鰭笛鯛與海鱺成長的影響

Abstract

本研究探討添加不同的重組類胰島素成長因子(Insulin-like growth factors, IGFs），與植物性原料替代魚粉飼料後對赤鰭笛鯛與海鱺幼魚的成長影響。研究分為兩個部分，分別是赤鰭笛鯛與海鱺兩種不同試驗魚種。以每100g飼料中添加重組類胰島素成長因子第一型、第二型(IGF-I, IGF-II）的大腸桿菌(E.coli）粉末0.5g、0.018g，以及酵母菌(Candida utilitus）的重組類胰島素成長因子第二型(IGF-II）粉末1.5g。其濃度為每1g 大腸桿菌含有 0.4、6mg 的IGF-I與IGF-II；酵母菌每1g含有0.4mg的IGF-II。 實驗一、二，探討赤鰭笛鯛飼料在含有60%魚粉，以及無魚粉與25%低含量魚粉的飼料組別中，添加不同重組IGFs。實驗一的結果顯示以全魚粉組中添加E. coli生產的IGF-I 0.5g的增重率153%，飼料效率83%具有較佳效果。實驗二的結果顯示在低含量魚粉組中含有E.coli生產的IGFs，其增重率為144、141%，飼料效率為75、71%，成長效果相當於全魚粉組試驗各組。 實驗三、四，探討海鱺飼料中添加不同重組類胰島素成長因子在60%魚粉組，以及植物性原料飼料試驗各組的成長影響。實驗三的結果顯示60%魚粉組中添加E. coli生產的IGF-I粉末0.5g的增重率168 %，飼料效率86%，相較於對照組有較佳效果。實驗四的結果顯示無魚粉組中以E. coli粉末生產重組的IGFs之增重率分別是86%、78%，相較於對照組的增重率64%，具有顯著性的效果；飼料效率各組在38－47%，各組之間未達顯著水準。

The fact that insulin-like growth factors (IGFs) can enhance the growth of fish is clearly demonstrated. The experiments were conducted to study the effects of partially substituting fishmeal with vegetative ingredients and IGFs in diet of juvenile Red striped snapper, Lutjanus erythropterus and cobia, Racycentron canadum. Experiment I investigated fish meal (60%) supplement to insulin-like growth factors of tilapia procreate form recombinant E. coli IGF-I, IGF-II and yeast(Candida utilitus) IGF-II in diet of juvenile Red striped snapper. The result of this experiment is that to feed the diet to add IGF-I contained in E.coli 0.5g per 100g diet has the best weight gain 153% and feed efficiency 83%. Experiment II studied the effect of feed supplement with Insulin-like growth factors of tilapia procreate form recombinant E. coli IGF-I, IGF-II and yeast IGF-II and partially substituting fishmeal with vegetative ingredients in diet of juvenile Red striped snapper. The result of this experiment is that to feed the diet to add IGF-I contained in E.coli 0.5g per 100g diet has the best weight gain 144% and feed efficiency 75%. Experiment III investigated fish meal (60%) supplement to insulin-like growth factors of tilapia procreate form recombinant E. coli IGF-I, IGF-II and yeast IGF-II in diet of juvenile cobia. The result of this experiment is that to feed the diet to add IGF-I contained in E.coli 0.5g per 100g diet has the best weight gain 168% and feed efficiency 86%. Experiment IV studied the effect of feed supplement with Insulin-like growth factors of tilapia procreate form recombinant E. coli IGF-I, IGF-II and yeast IGF-II and partially substituting fishmeal with vegetative ingredients in diet of juvenile cobia. The result of this experiment is that to feed the diet to add IGF-I contained in E.coli 0.5g per 100g diet has the best weight gain 86% and feed efficiency 47%.