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標題: | 阿拉伯芥中 Myo-Inositol Oxygenase 4 (MIOX4)基因對於長期熱處理下的影響 Functional Characterization of Myo-Inositol Oxygenase 4 in Arabidopsis thialana During Long-Term Heat Stress |
作者: | I-Chu Tsai 蔡亦筑 |
指導教授: | 鄭石通(Shih-Tong Jeng) |
關鍵字: | MIOX4,維他命 C,高溫,花型態, MIOX4,vitamin C,heat stress,flower phenotype, |
出版年 : | 2011 |
學位: | 碩士 |
摘要: | 全球溫室效應對植物造成傷害也降低了作物的產量。研究發現在高溫下,許多重要作物的雄蕊生成、花粉存活率,種子萌芽率、根的生長及花的發育等都受到影響。從微陣列的資料顯示出MIOX4 (Myo-inositol oxygenase 4) 的表現量在高溫時會下降。已知MIOX4 參與在核苷酸醣類的代謝,並且可以影響植物中維他命C的含量。而維他命C的含量與植物抗逆境及花和果實的發育相關。為了研究在長期高溫下MIOX4對植物的影響,我們構築了大量表現MIOX4的轉殖株及篩選出抑制MIOX4表現的T-DNA轉殖株。我們發現大量表現轉殖株在小苗時有較長的主根長度,在長時間高溫、鹽和滲透壓逆境下幫助植物的種子萌芽和根的生長。而大量表現的轉殖株中維他命C的含量較高,而H2O2的含量則比野生型明顯較低。在花型態的觀察,發現大量表現的轉殖株有較多的花及果莢,但是花藥變異使花粉不易散出,而花粉萌發率及種子數在常溫及長期高溫下都比野生型來的少,而表現抑制的轉殖株和野生型則沒有太大差異。目前推測ROS的含量可能在花型態發育也扮演一種要角色。這些結果顯示出,MIOX4在小苗時幫助植物在非生物逆境更具耐受性,但在長期高溫中,MIOX4的表現會使花的發育受損。 Global warming damages plants and decreases crop yield. In crops, high temperature influences the anther formation, pollen viability, seed germination, root growth, and flower development. From the microarray data, MIOX4 (Myo-inositol oxygenase 4) expression was reduced under high temperature. MIOX4 influences the nucleotide sugar metabolism and the level of ascorbic acid that both affect flower and fruit development. In order to study the effects of long-term heat stress, Arabidopsis over-expressing MIOX4 and plants with miox4 were obtained. The over-expression lines displayed the increase of ascorbic acid and the decrease of hydrogen peroxide, and their root lengths wew affected Mutant miox4 showed lower germination rate under temperature stress. The over-expression lines displayed the higher and miox4 exhibited the lower seed germination rate than wild type under osmotic stress. In the reproduction stage, the over-expression lines produced more flowers and siliques than wild type did, but showed the lower pollen germination rate and yield. Under long-term heat stress, the reduction in pollen germination and yield of the over-expression lines was more remarkable. We assumed the contents of ROS play important roles at the flower development. Conclusively, the seedlings of plants over-expressing MIOX4 can tolerate high osmotic stress, but impair flower development. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25818 |
全文授權: | 未授權 |
顯示於系所單位: | 植物科學研究所 |
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