請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/2575
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 陳凱儀(Kai-Yi Chen) | |
dc.contributor.author | Pin-Yao Chen | en |
dc.contributor.author | 陳品堯 | zh_TW |
dc.date.accessioned | 2021-05-13T06:42:21Z | - |
dc.date.available | 2020-01-04 | |
dc.date.available | 2021-05-13T06:42:21Z | - |
dc.date.copyright | 2018-01-04 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-10-17 | |
dc.identifier.citation | Chen KY, Tanksley SD. (2004) High-Resolution Mapping and Functional Analysis of se2.1: A Major Stigma Exsertion Quantitative Trait Locus Associated With the Evolution From Allogamy to Autogamy in the Genus Lycopersicon. Genetics 168: 1563–1573.
Daminato M, Masiero S, Resentini F, Lovisetto A, Casadoro G. (2014) Characterization of TM8, a MADS-box gene expressed in tomato flowers. BMC Plant Biology 14:319 Dobritzsch S, Weyhe M, Schubert R, Dindas J, Hause G, Kopka J, Hause B (2015) Dissection of jasmonate functions in tomato stamen development by transcriptome and metabolome analyses. BMC Biology 13:28 Gates DJ, Strickler SR, Mueller LA, Olson BJSC, Smith SD. (2016) Diversification of R2R3-MYB Transcription Factors in the Tomato Family Solanaceae. Journal of Molecular Evolution 83:26–37 Kiferle C, Fantini E, Bassolino L, Povero G, Spelt C, Buti S, Giuliano G, Quattrocchio F, Koes R, Perata P, Gonzali S. (2015) Tomato R2R3-MYB Proteins SlANT1 and SlAN2: Same Protein Activity, Different Roles. PLoS ONE 10(8): e0136365. Lee SB, Suh MC. (2015) Cuticular wax biosynthesis is up-regulated by the MYB94 transcription factor in Arabidopsis. Plant Cell Physiol 56(1): 48–60 Mahjoub A, Hernould M, Joube` JR, Decendit A, Mars M, Barrieu F, Hamdi S, Delrot S. (2009) Overexpression of a grapevine R2R3-MYB factor in tomato affects vegetative development, flower morphology and flavonoid and terpenoid metabolism. Plant Physiology and Biochemistry 47 551–561 Meng X, Yang D, Li X, Zhao S, Sui N, Meng Q. (2015) Physiological changes in fruit ripening caused by overexpression of tomato SlAN2, an R2R3-MYB factor. Plant Physiology and Biochemistry 89 24-30 Müller F, Xu J, Kristensen L, Wolters-Arts M , de Groot PFM, Jansma SY, Mariani1 C, Park S, Rieu I. (2016) High-Temperature-Induced Defects in Tomato (Solanum lycopersicum) Anther and Pollen Development Are Associated with Reduced Expression of B-Class Floral Patterning Genes. PLoS ONE 11(12): e0167614. Quinet M, Bataille G, Dobrev PI, Capel C , Gómez P, Capel J, Lutts S, Motyka V, Angosto T, Lozano R. (2014) Transcriptional and hormonal regulation of petal and stamen development by STAMENLESS, the tomato (Solanum lycopersicum L.) orthologue to the B-class APETALA3 gene. Journal of Experimental Botany Vol. 65, No. 9, pp. 2243–2256 Zhao P, Li Q, Li J, Wang L, Ren Z. (2014) Genome wide identification and characterization of R2R3MYB family in Solanum lycopersicum. Molecular Genetics and Genomics 289:1183–1207 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/2575 | - |
dc.description.abstract | 番茄的se2.1數量性狀基因座為複合式數量性狀基因座,包含style2.1、stamen2.1、stamen2.2、stamen2.3、以及dehiscence2.1,其中的stamen2.2的候選基因定位在番茄第二對染色體上cLED19A24至CT9分子標記區間內。本研究選擇位於此染色體區間內的SlMYB94基因為stamen2.2的候選基因,並藉由基因轉殖的方式,觀察SlMYB94對偶基因數目的增加,是否造成番茄雄蕊與雌蕊長度的改變。轉殖植株的初步篩選是採用CaMV 35S分子標記和番茄背景分子標記對轉殖T_0世代植株進行篩選,T_1自交世代個別植株的基因型則藉由焦磷酸定序 (pyrosequencing) 所偵測轉殖對偶基因型與背景對偶基因型之單核苷多型性的比例推算而得。試驗結果顯示T_0世代轉殖株「中研院2-9」成功轉殖單一SlMYB94對偶基因,然而在其自交世代16TS357族群中,帶有不同數目轉殖對偶基因植株之間的雄蕊與雌蕊長度並沒有顯著的差異。由此結果推論SlMYB94並非stamen2.2基因。 | zh_TW |
dc.description.abstract | The tomato se2.1 is a composite quantitative trait locus, including style2.1, stamen2.1, stamen2.2, stamen2.3, and dehiscence2.1. The stamen2.2 was delimited between molecular markers cLED19A24 and CT9 on the chromosome 2. In the current study, the SlMYB94 gene is one of genes residing in the aforementioned chromosomal region and was chosen as the candidate gene of stamen2.2. The correlation between the SlMYB94 copy number and the changes of stamen and style length were observed via the gene transformation approach. CaMV 35S marker and genetic background marker were used to identify successful transgenic plants in the T_0 generation. In the T_1 generation, pyrosequencing was used to detect the proportion of single nucleotide polymorphism between the transformed allele and the background allele, and hence to infer genotypes of T_1 plants. The results showed that the “sinica2-9” T_0 plant successfully received a single copy of the SlMYB94 transgenic allele but its T_1 progenies, the 16TS357 population, did not show significant difference on style length and stamen length between individuals carrying different copy number of the SlMYB94 transgenic alleles. This conclusion infers that the SlMYB94 gene is not stamen2.2. | en |
dc.description.provenance | Made available in DSpace on 2021-05-13T06:42:21Z (GMT). No. of bitstreams: 1 ntu-106-R04621121-1.pdf: 2909872 bytes, checksum: 873db544fae6e4b5f0d1e7b303e9d4e5 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 誌謝 ....................................i
中文摘要 ....................................ii ABSTRACT ....................................iii 目錄 ....................................iv 圖目錄 ....................................vi 表目錄 ....................................vii 第一章 前言.................................... .1 第一節 Style 2.1與Stamen 2.2的候選基因MYB94.......1 第二節 影響番茄花器長度的基因及轉錄因子MYB...........3 第二章 研究目的 .................................4 第三章 材料與方法 .........................5 第一節 試驗材料 .................................5 第二節 抽取番茄基因體DNA .........................8 第三節 DNA純化 .................................10 第四節 清洗種子及乾燥保存 .........................11 第五節 初步確認轉殖是否成功及轉殖背景的分子標記 .......11 第六節 焦磷酸定序(pyrosequencing) .........14 第七節 雌蕊及雄蕊長度調查 .........................16 第八節 統計分析 .................................16 第四章 結果 .................................18 第一節 轉殖世代T0之轉殖與背景檢測及自交後代T1 .18 第二節 轉殖自交後代T1花器長度之測量 .........25 第三節 焦磷酸定序結果 .........................27 第四節 基因型分析 .........................32 第五章 討論 .................................38 第一節 35S分子標記與焦磷酸定序結果不完全相同 .........38 第二節 SlMYB94基因可能並非期望的stamen2.2基因 .39 引用文獻 .........................................40 附錄 .........................................42 附件1 .........................................42 附件2 .........................................45 附件3 .........................................48 附件4 .........................................49 附件5 .........................................54 附件6 .........................................61 附件7 .........................................62 附件8 .........................................64 | |
dc.language.iso | zh-TW | |
dc.title | SlMYB94轉殖基因對番茄花器長度的影響評估 | zh_TW |
dc.title | Assessing Effect of the Transgenic SlMYB94 Allele on Floral Organ Length in Tomato | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 董致韡(Chih-Wei Tung),蔡育彰(Yu-Chang Tsai),蔡欣甫(Shin-Fu Tsai) | |
dc.subject.keyword | 番茄,se2.1,stamen2.2,MYB,焦磷酸定序,雄蕊長, | zh_TW |
dc.subject.keyword | tomato,se2.1,stamen2.2,MYB,pyrosequencing,stamen length, | en |
dc.relation.page | 64 | |
dc.identifier.doi | 10.6342/NTU201704293 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2017-10-18 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-1.pdf | 2.84 MB | Adobe PDF | 檢視/開啟 |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。