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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 陳美如 | |
dc.contributor.author | kun-Liang Chen | en |
dc.contributor.author | 陳坤良 | zh_TW |
dc.date.accessioned | 2021-06-08T06:28:00Z | - |
dc.date.copyright | 2006-08-04 | |
dc.date.issued | 2006 | |
dc.date.submitted | 2006-07-26 | |
dc.identifier.citation | Baer, R., Bankier, A. T., Biggin, M. D., Deininger, P. L., Farrell, P. J., Gibson, T. J., Hatfull, G., Hudson, G. S., Satchwell, S. C., Seguin, C. & et al. (1984). DNA sequence and expression of the B95-8 Epstein-Barr virus genome. Nature 310, 207-11.
Bieleski, L. & Talbot, S. J. (2001). Kaposi's sarcoma-associated herpesvirus vCyclin open reading frame contains an internal ribosome entry site. J Virol 75, 1864-9. Borman, A. M., Kirchweger, R., Ziegler, E., Rhoads, R. E., Skern, T. & Kean, K. M. (1997). elF4G and its proteolytic cleavage products: effect on initiation of protein synthesis from capped, uncapped, and IRES-containing mRNAs. Rna 3, 186-96. Borman, A. M., Le Mercier, P., Girard, M. & Kean, K. M.(1997). Comparison of picornaviral IRES-driven internal initiation of translation in cultured cells of different origins. Nucleic Acids Res 25, 925-32. Burkitt, D. (1958). A sarcoma involving the jaws in African children. Br J Surg 46, 218-23. Chang, P. J. & Liu, S. T. (2001). Function of the intercistronic region of BRLF1-BZLF1 bicistronic mRNA in translating the zta protein of Epstein-Barr virus. J Virol 75, 1142-51. Chang, Y., Tung, C. H., Huang, Y. T., Lu, J., Chen, J. Y. & Tsai, C. H. (1999).Requirement for cell-to-cell contact in Epstein-Barr virus infection of nasopharyngeal carcinoma cells and keratinocytes. J Virol 73, 8857-66. Chen,M. R., Hsu, T. Y., Chen, J. Y. & Yang, C. S. (1990). Molecular characterization of a cDNA clone encoding the Epstein-Barr virus (EBV) DNase. J Virol Methods 29, 127-41. Chen, M. R., Chang, S. J., Huang, H. & Chen, J. Y. (2000). A protein kinase activity associated with Epstein-Barr virus BGLF4 phosphorylates the viral early antigen EA-D in vitro. J Virol 74, 3093-104. Colem an, H. M., Brierley, I. & Stevenson, P. G. (2003). An internal ribosome entry site directs translation of the murine gammaherpesvirus 68 MK3 open reading frame. J Virol 77, 13093-105. de Jesus, O., Smith, P. R., Spender, L. C., Elgueta Karstegl, C., Niller, H. H., Huang, D. & Farrell, P. J. (2003). Updated Epstein-Barr virus (EBV) DNA sequence and analysis of a promoter for the BART (CST, BARF0) RNAs of EBV. J Gen Virol 84, 1443-50. Epstein, M. A., Achong, B. G. & Barr, Y. M. (1964). Virus Particles in Cultured Lymphoblasts from Burkitt's Lymphoma. Lancet 15, 702-3. Grundhoff, A. & Ganem, D. (2001). Mechanisms governing expression of the v-FLIP gene of Kaposi's sarcoma-associated herpesvirus. J Virol 75, 1857-63. Henle, G. & Henle, W. (1966). Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol 91, 1248-56. Henle, G., Henle, W. & Diehl, V. (1968). Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc Natl Acad Sci U S A 59, 94-101. Holcik, M., Sonenberg, N. & Korneluk, R. G. (2000). Internal ribosome initiation of translation and the control of cell death. Trends Genet 16, 469-73. Isaksson, A., Berggren, M. & Ricksten, A. (2003). Epstein-Barr virus U leader exon contains an internal ribosome entry site. Oncogene 22, 572-81. Isler, J. A., Skalet, A. H. & Alwine, J. C. (2005). Human cytomegalovirus infection activates and regulates the unfolded protein response. J V irol 79, 6890-9. Jang, S. K., Davies, M. V., Kaufman, R. J. & Wimmer, E. (1989). Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo. J Virol 63, 1651-60. Jang, S. K., Krausslich, H. G., Nicklin, M. J., Duke, G. M., Palmenberg, A. C. & Wimmer, E. (1988). A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. J Virol 62, 2636-43. Kato, K Yokoyama, A., Tohya, Y., Akashi, H., Nishiyama, Y. & Kawaguchi, Y. ., (2003). Identification of protein kinases responsible for phosphorylation of Epstein-Barr virus nuclear antigen leader protein at serine-35, which regulates its coactivator function. J Gen Virol 84, 3381-92. Kato, K., Yokoyama, A., Tohya, Y., Akashi, H., Nishiyama, Y. & Kawaguchi, Y. . (2003). Identification of protein kinases responsible for phosphorylation of Epstein-Barr virus nuclear antigen leader protein at serine-35, which regulates its coactivator function. J Gen Virol 84, 3381-92. Kawaguchi, Y., Kato, K., Tanaka, M., Kanamori, M., Nishiyama, Y. & Yamanashi, Y. (2003). Conserved protein kinases encoded by herpesviruses and cellular protein kinase cdc2 target the same phosphorylation site in eukaryotic elongation factor 1delta. J Virol 77, 2359-68. Kieft, J. S., Zhou, K., Grech, A., Jubin, R. & Doudna, J. A. (2002). Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation. Nat Struct Biol 9, 370-4. Kikuta, H., Taguchi, Y., Tomizawa, K., Kojima, K., Kawamura, N., Ishizaka, A., Sakiyama, Y., Matsumoto, S., Imai, S., Kinoshita, T. & et al. (1988). Epstein-Barr virus genome-positive T lymphocytes in a boy with chronic active EBV infection associated with Kawasaki-like disease. Nature 333, 455-7. Kim, Y. K., Lee, S. H., Kim, C. S., Seol, S. K. & Jang, S. K. (2003). Long-range RNA-RNA interaction between the 5' nontranslated region and the core-coding sequences of hepatitis C virus modulates the IRES-dependent translation. Rna 9, 599-606. Kolupaeva, V. G., Pestova, T. V., Hellen, C. U. & Shatsky, I. N. (1998). Translation eukaryotic initiation factor 4G recognizes a specific structural element within the internal ribosome entry site of encephalomyocarditis virus RNA. J Biol Chem 273, 18599-604. Le, S. Y., Sonenberg, N. & Maizel, J. V., Jr. (1995). Unusual folding regions . and ribosome landing pad within hepatitis C virus and pestivirus RNAs. Gene 154, 137-43. Lin, C. T., Chan, W. Y., Chen, W., Huang, H. M., Wu, H. C., Hsu, M. M., Chuang, S. M. & Wang, C. C. (1993). Characterization of seven newly established nasopharyngeal carcinoma cell lines. Lab Invest 68, 716-27. Lin, J. C., Smith, M. C. & Pagano, J. S. (1981). Induction of replication of Epstein-Barr virus DNA by 12-O-tetradecanoyl-phorbol-13-acetate. II.Inhibition by retinoic acid and 9-(2-hydroxyethoxymethyl) guanine. Virology 111, 294-8. Low, W., Harries, M., Ye, H., Du, M. Q., Boshoff, C. & Collins, M. (2001). Internal ribosome entry site regulates translation of Kaposi's sarcoma-associated herpesvirus FLICE inhibitory protein.J Virol 75, 2938-45. Luka, J., Kallin, B . & Klein, G. (1979). Induction of the Epstein-Barr virus (EBV) cycle in latently infected cells by n-butyrate. Virology 94, 228-31. Macejak, D. G. & Sarnow, P. (1991) . Internal initiation of translation mediated by the 5' leader of a cellular mRNA. Nature 353, 90-4. Ohlma nn, T., Pain, V. M., Wood, W., Rau, M. & Morley, S. J. (1997). The proteolytic cleavage of eukaryotic initiation factor (eIF) 4G is prevented by eIF4E binding protein (PHAS-I; 4E-BP1) in the reticulocyte lysate. Embo J 16, 844-55. Pelletier, J. & Sonenberg, N . (1988). Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature 334, 320-5. Reynolds, J. E., Kaminski, A., Kettinen, H. J., Grace, K., Clarke, B. E., Carroll, A. R., Rowlands, D. J. & Jackson, R. J. (1995). Unique features of internal initiation of hepatitis C virus RNA translation. Embo J 14, 6010-20. Schang, L. M., Rosenberg, A. & Schaffer, P. A. (1999). Transcription of herpes simplex virus immediate-early and early genes is inhibited by roscovitine, an inhibitor specific for cellular cyclin-dependent kinases. J Virol 73, 2161-72 . Sherrill, K. W., Byrd, M. P., Van Eden, M. E. & Lloyd, R. E. (2004). BCL-2 translation is mediated via internal ribosome entry during cell stress. J Biol Chem 279, 29066-74. Shimizu, N., Tanabe-Tochikura, A., Kuroiwa, Y. & Takada, K. (1994). Isolation of Epstein-Barr virus (EBV)-negative cell clones from the EBV-positive Burkitt's lymphoma (BL) line Akata: malignant phenotypes of BL cells are dependent on EBV. J Virol 68, 6069-73. Sixbey, J. W., Vesterinen, E. H., Nedrud, J. G., Raab-Traub, N., Walton, L. A. & Pagano, J. S. (1983). Replication of Epstein-Barr virus in human epithelial cells infected in vitro. Nature 306, 480-3. Sizova , D. V., Kolupaeva, V. G., Pestova, T. V., Shatsky, I. N. & Hellen, C. U. (1998). Specific interaction of eukaryotic translation initiation factor 3 with the 5' nontranslated regions of hepatitis C virus and classical swine fever virus RNAs. J Virol 72, 4775-82. Takada, K. (1984). Cross-linking of cell surface immunoglobulins induces Epstein-Barr virus in Burkitt lymphoma lines. Int J Cancer 33, 27-32,. Tovey M. G., Lenoir, G. & Begon-Lours, J. (1978). Activation of latent Epstein-Barr virus by antibody to human IgM. Nature 276, 270-2. Tsukiyama-Kohara, K., Iizuka, N., Kohara, M. & Nomoto, A. (1992).Internal ribosome entry site within hepatitis C virus RNA. J Virol 66,1476-83. Yue, W ., Gershburg, E. & Pagano, J. S. (2005). Hyperphosphorylation of EBNA2 by Epstein-Barr virus protein kinase suppresses transactivation of the LMP1 promoter. J Virol 79, 5880-5. . | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/25748 | - |
dc.description.abstract | BGLF4是EB病毒在進入溶裂期時表現的serine/threonine蛋白激酶,目前已知BGLF4的受質包含有:BMRF1、Zta、EBNA-LP、EF-1δ等。已知BGLF4存在於病毒DNA複製區以及成熟病毒顆粒中,推測BGLF4在病毒的複製過程以及殼體化(encapsidation)中扮演重要的角色。我們實驗室先前利用5’RACE在BGLF4開放譯讀架上游201 bp和255 bp的位置找到其轉錄起始位點。而在本篇論文中除了先前已知的轉錄起始點外,我們在BGLF4開放譯讀架上游472 bp位置上發現另一轉錄起始點。而對於BGLF4長片段的5’端未轉譯區,我們進一步的探討是否具有內部核糖體進入位點(Internal Ribosome Entry Site , IRES)的調控機制。藉由雙譯讀架(bicistronic reporter)DNA以及RNA短暫轉染至293T細胞中證明BGLF4的5’端未轉譯區含有IRES的活性。另外本篇論文中發現BGLF4基因的IRES活性會受到內質網壓力(ER Stress)反應刺激增加,同時我們在EBV positive Akata細胞中觀察到EB病毒的再活化(reactivation)會使細胞產生ER Stress。而在NA細胞中,當利用Rta刺激EB病毒進入溶裂期後BGLF4基因IRES活性會因為EB病毒進入溶裂期而增加。表示BGLF4 5’端未轉譯區上的IRES對於EB病毒在進入溶裂期時能夠幫助BGLF4蛋白激酶的表現。 | zh_TW |
dc.description.abstract | Epstein-Barr virus (EBV) BGLF4 encodes the serine/threonine protein kinase which can phosphorylate several viral substrates including BMRF1, Zta, EBNA-LP and cellular translation factor EF-1d. BGLF4 was detected in viral DNA replication compartment and mature virion, suggesting it may play roles in regulating virus DNA replication and encapsidation or egress process. In our previous study, two transcription start sites at -255 and -201 upstream of translation initiation site were identified in 5’-RACE analysis. In this study, we used another primer sets to further identify another transcription start sites of BGLF4 at -472 nt, which is 25 nt downstream of a putative TATA box. To examine whether the long 5’ untranslated region (UTR) of BGLF4 might function as the internal ribosome entry site (IRES), dual luciferase reporter assay was performed with DNA or RNA transfection to demonstrate the translation enhancement effect of BGLF4 5’-UTR. We further demonstrated the BGLF4 IRES activity was enhanced under tunicamycin treatment which would induce the ER stress. Additionally, induction of ER stress was observed upon EBV reactivation in anti-IgG induced EBV positive Akata cells as revealed by the detection of the spliced form of XBP-1. Simultaneously, BGLF4 IRES activity was enhanced upon EBV reaction induced by Rta in EBV-positive NA cells. Data here thus suggest the 5’-UTR of BGLF4 may ensure BGLF4 expression while the cap-dependent translation machinery is blocked in lytic virus replicating cells. | en |
dc.description.provenance | Made available in DSpace on 2021-06-08T06:28:00Z (GMT). No. of bitstreams: 1 ntu-95-R93445118-1.pdf: 731761 bytes, checksum: ddb761c271519db727c7ff39801d2b82 (MD5) Previous issue date: 2006 | en |
dc.description.tableofcontents | 目錄
中文摘要………………………………………………………………2 英文摘要………………………………………………………………3 前言……………………………………………………………………4 研究目標………………………………………………………………10 材料與方法……………………………………………………………11 實驗結果………………………………………………………………21 討論……………………………………………………………………28 圖表……………………………………………………………………32 參考文獻………………………………………………………………48 | |
dc.language.iso | zh-TW | |
dc.title | EB病毒蛋白質激酶BGLF4
轉譯調控機制之探討 | zh_TW |
dc.title | Translational Regulation of EBV BGLF4 Protein Kinase | en |
dc.type | Thesis | |
dc.date.schoolyear | 94-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 蔡錦華,張鑫,林素芳 | |
dc.subject.keyword | EB病毒, | zh_TW |
dc.subject.keyword | BGLF4,IRES, | en |
dc.relation.page | 54 | |
dc.rights.note | 未授權 | |
dc.date.accepted | 2006-07-27 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 微生物學研究所 | zh_TW |
顯示於系所單位: | 微生物學科所 |
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